Compositions and methods for producing food products with recombinant animal protein

ABSTRACT

The invention relates to methods and compositions for the recombinant production of animal protein for use in animal food, particularly pet food.

1. CROSS REFERENCE TO RELATED APPLICATIONS

This application claims priority under 35 USC § 119(e) to U.S. Provisional Patent Application No. 62/798,447 filed on Jan. 29, 2019, which is incorporated by reference in its entirety.

2. SEQUENCE LISTING

Incorporated by reference in its entirety herein is a computer-readable nucleotide/amino acid sequence listing submitted herewith and identified as follows: 1,777,447 Byte ASCII (Text) file name “513482_ST25” created on Mar. 18, 2022.

3. BACKGROUND

Currently, the production of high-quality protein foods and feed includes animal meat. As the global human and companion animal populations increase, the demand for high-quality protein food is expected to increase. However, obtaining proteins from animal meat for food production is an environmentally demanding, and potentially destructive, process.

While plant sources, e.g. legumes, contain a significant amount of protein, they often lack one or more essential amino acids for many mammalian diets [4] or they not as bioavailable as animal protein, making plant protein insufficient or sub-optimal alternative for many food applications. In fact, tryptophan and lysine are scarce in corn, lysine in wheat and other cereals, and methionine in soybeans and other legumes [6]. In addition, plant sources also contain anti-nutritional factors like fiber, phytate, and protease inhibitors, that limit digestion and absorption [1],[2]. Soybean, a commonly used protein source, decreases the digestibility in canine foods when present in concentrations over 15% [3]. Moreover, humans and companion animals have different amino acid requirements.

Hence, there remains a need for a source of proteins that do not come from animal meat yet satisfy the growing demand for high-quality protein food products and deliver on a multitude of nutritional needs.

4. SUMMARY

Disclosed herein are food compositions made with recombinantly produced animal proteins and methods for producing the recombinant animal proteins. Nucleic acid molecules encoding the animal proteins, expression vectors, recombinant host cells, and methods for making the animal proteins are also provided.

The recombinantly-produced animal proteins of the disclosure can be incorporated into food or feed product as whole cells, protein concentrates from cell lysates and/or cell supernatants, or as protein isolates to make various food products (e.g., primary diet foods, secondary diet foods), intermediate food products, supplements, and pharmaceutical compositions. The recombinant animal protein compositions may be mixed with other ingredients, shaped into a suitable form factor, to generate food products with a taste and mouthfeel suitable for humans or companion animals (e.g., dogs, cats, ferrets and the like).

Disclosed herein are improved methods and compositions for manufacturing food for animals, particularly companion animals. In certain embodiments, the methods entail producing animal proteins recombinantly in a microbial host, as described herein. The recombinant proteins produced by the method can provide equivalent or better nutrition than conventionally harvested animal proteins or plant-derived proteins, without the associated deficiencies described above. In certain embodiments, the recombinant animal proteins described herein can also be incorporated into or serve as food for humans, wild animals, livestock, domestic pets, companion animals, and/or zoo animals. In preferred embodiments, the food composition is substantially free of antibiotics, animal growth hormones, and/or meat from farmed, caught or slaughtered animals.

One or a plurality of recombinant proteins can be produced in one organism, or one strain, thereby allowing the amino acid profile to be tailored to the particular nutritional needs of targeted companion and other animals, including humans. Alternatively, a single recombinant animal protein can be produced in one strain (or organism) and mixed with a protein or proteins produced in a different strain (or organism) to yield a final product with the desired proportions of amino acids and other nutrients. Thus, the amino acid profile (and/or the profile of other nutrients) can be customized for the targeted animal, including pets and humans.

5. BRIEF DESCRIPTION OF THE DRAWINGS

FIG. 1 is a photograph of an SDS-PAGE gel of proteins extracted from an S. cerevisiae host cell strain that expresses a chicken cofilin-2 protein, with the chicken cofilin-2 band identified.

FIG. 2 shows the growth curves of an S. cerevisiae host cell strain that expresses chicken cofilin-2, and a control S. cerevisiae strain that does not express chicken cofilin-2, each grown under two different media conditions, (i) raffinose alone or (ii) raffinose with galactose to induce protein expression.

FIG. 3 shows maximum specific growth rates (μmax [h⁻¹]) from the experiments shown in FIG. 2 . The error bars shown are standard deviation.

FIG. 4 is a picture of an SDS-PAGE gel of proteins extracted from an S. cerevisiae host cell strain that expresses chicken profilin protein, with the profilin band identified.

FIG. 5 is a picture of an SDS-PAGE gel of proteins extracted from an S. cerevisiae host cell strain that expresses chicken profilin protein that was used to make theoretical calculation shown in Table 4.

FIG. 6 shows the growth curve of an S. cerevisiae host cell strain expressing chicken profilin, and a control S. cerevisiae strain that does not express chicken profilin, each grown under two different media conditions, (i) raffinose alone or (ii) raffinose with galactose to induce protein expression.

FIG. 7 shows maximum specific growth rates (μmax [h⁻¹]) from the experiments shown in FIG. 6 . The error bars are standard deviation. The asterisk denotes P value <0.05

FIG. 8 shows a picture of dried pellet from whole-cells expressing a recombinant profilin protein from chicken.

FIG. 9 shows a picture of the mixed, dry ingredients with a recombinant chicken profilin protein, processed into a powder.

FIGS. 10A-10B show pictures of processing a dough containing a recombinant animal protein into a food product. 10A shows a picture of processing of the wet and dry ingredients into a dough. 10B shows a picture of the molding the dough into a form factor of a treat.

FIGS. 11A-C show a picture of the dried and packaged treat with different protein content.

FIG. 12 shows a picture of an SDS-PAGE gel of a chicken coronin protein expressed in an S. cerevisiae host cell strain.

FIG. 13 shows a picture of an SDS-PAGE gel of a turkey myozenin-1 protein expressed in an S. cerevisiae host cell strain.

FIG. 14 shows a picture of an SDS-PAGE gel of a pig troponin C protein expressed in an S. cerevisiae host cell strain.

FIG. 15 shows a picture of an SDS-PAGE gel of a chicken cofilin-2 protein expressed in a K. phaffii host cell strain.

FIG. 16 shows a picture of an SDS-PAGE gel of a chicken profilin protein expressed in a K. phaffi host cell strain.

FIG. 17 shows a picture of an SDS-PAGE gel of chicken profilin protein expressed in a K. lactis host cell strain.

FIG. 18 shows a picture of an SDS-PAGE gel of a chicken profilin protein expressed in an S. pombe host cell strain.

6. DETAILED DESCRIPTION OF THE INVENTION 6.1. Definitions

Unless otherwise defined herein, all technical and scientific terms used herein have the meaning commonly understood by a person skilled in the art to which this invention pertains.

The term “ameliorating” refers to any therapeutically beneficial result in the treatment of a disease state, e.g., a nutritional deficiency disease state, including prophylaxis, lessening in the severity or progression, remission, or cure thereof.

The term “mammal” as used herein includes both humans and non-humans and includes but is not limited to humans, non-human primates, canines, felines, murines, bovines, equines, birds, and porcines.

The term “percent identity”, in the context of two or more nucleic acid or polypeptide sequences, refers to a specified percentage of nucleotides or amino acid residues that are identical as between or as among the sequences when aligned for maximum correspondence. Optimal alignment of sequences for comparison can be conducted, e.g., by the local homology algorithm of Smith & Waterman, Adv. Appl. Math. 2:482 (1981), by the homology alignment algorithm of Needleman & Wunsch, J. Mol. Biol. 48:443 (1970), by the search for similarity method of Pearson & Lipman, Proc. Nat'l. Acad. Sci. USA 85:2444 (1988), by computerized implementations of these algorithms (GAP, BESTFIT, FASTA, and TFASTA in the Wisconsin Genetics Software Package, Genetics Computer Group, 575 Science Dr., Madison, Wis.), or by visual inspection (see generally Ausubel et al., infra) Unless otherwise specified, “percent identity” is assessed herein using the BLAST algorithm, which is described in Altschul et al., J. Mol. Biol. 215:403-410 (1990). Software for performing BLAST analyses is publicly available through the National Center for Biotechnology Information (www.ncbi.nlm.nih.gov/), and unless otherwise specified, “percent identity” is measured using BLASTP or BLASTN with default parameters at (www.ncbi.nlm.nih.gov). Depending on the application, the percent “identity” can exist over a region (e.g. a fragment) of the sequence being compared, e.g., over a functional domain, or, alternatively, exist over the full length of the two sequences to be compared.

The term “sufficient amount” means an amount sufficient to produce a desired effect, e.g., an amount sufficient to modulate protein aggregation in a cell.

The term “therapeutically effective amount” is an amount that is effective to ameliorate a symptom of a disease. A therapeutically effective amount can be a “prophylactically effective amount” as prophylaxis can be considered therapy.

The term “nutritional supplement,” as used herein, generally refers to a substance capable of supplementing a diet of a human, dog, cat, or other animal. A nutritional supplement may provide essential nutrients (e.g., vitamins, minerals, macronutrients, trace nutrients, and/or cofactors). A nutritional supplement may be a dietary supplement.

The term “flavoring agent,” as used herein, generally refers to a substance capable of altering a flavor of a food product. A flavoring agent may include a flavoring molecule(s) or precursor(s), such as, for example, carbohydrates (e.g., sugar), sweeteners, or salts.

The term “recombinant host cell” as used herein refers to a host cell(s) that have been genetically modified to express or overexpress endogenous polynucleotides, to express heterologous polynucleotides or polypeptides, such as those included in an expression vector, in an integration construct, or which have an alteration in expression of an endogenous gene. By “alteration” it is meant that the expression of the gene, or level of a RNA molecule or equivalent RNA molecules encoding one or more polypeptides or polypeptide subunits, or activity of one or more polypeptides or polypeptide subunits is up regulated or down regulated, such that expression, level, or activity is greater than or less than that observed in the absence of the alteration. For example, the term “alter” can mean “inhibit,” but the use of the word “alter” is not limited to this definition.

The term “heterologous” as used herein indicates molecules that are expressed in an organism other than the organism from which they originated or are found in nature. The molecule can have a coding region that is different from the host cell or a promoter region that is different from the host cell, or both.

On the other hand, the term “native” or “endogenous” as used herein indicates molecules that are expressed in the organism in which they originated or are found in nature, independently of the level of expression that can be lower, equal or higher than the level of expression of the molecule in the native host cell. It is understood that expression of wild-type enzymes or polynucleotides may be modified in recombinant host cells.

The term “transformation” refers to the transfer of a nucleic acid fragment into a host organism, resulting in genetic inheritance. Genetic inheritance can be stable or unstable. Host cells (e.g., eukaryotic cells) containing the transformed nucleic acid fragments are referred to as “transgenic” or “recombinant” or “transformed”.

The term “primary food product” or “primary diet food product” as used herein indicates a food product that is the core source of daily nutrition such as a complete meal or feed.

The term “secondary food product” or “secondary diet food product” as used herein indicates a food product that is generally not the core source of daily nutrition. By way of example, a secondary food product can be a snack, a treat, or an edible toy.

The term “intermediate food product” as used herein indicates a food product that is added to make the ultimate ingestible food composition. The intermediate food product is typically in a format that allows it to be mixed, coated, soaked, or injected to make the ultimate ingestible food composition.

The term “supplement” as used herein indicates a nutritional product that is intended to add or enhance the nutrient intake. The supplement can typically be in the form of a pill, a capsule, a tablet, a liquid, a soup, broth, or a dissolvable powder.

The term “substantially free” refers to a composition that comprises a desired compound, desired compounds, and inert compounds and is free of significant quantities of an undesired compound or undesired compounds. A typical substantially free composition comprises greater than about 80% by weight of the desired compound, desired compounds, and inert compounds and less than about 20% by weight of one or more other undesired compounds, more preferably greater than about 90% by weight of the desired compound, desired compounds, and inert compounds and less than about 10% by weight of one or more other undesired compounds, even more preferably greater than about 95% by weight of the desired compound, desired compounds, and inert compounds and less than about 5% by weight of one or more other undesired compounds, and most preferably greater than about 97% by weight of the desired compound, desired compounds, and inert compounds and less than about 3% by weight of one or more other undesired compounds.

The term “about” is used herein to mean approximately, in the region of, roughly, or around. When the term “about” is used in conjunction with a numerical range, it modifies that range by extending the boundaries above and below the numerical values set forth. In general, the term “about” is used herein to modify a numerical value above and below the stated value by a variance of 10%.

The term “robust protein expression” is used herein to mean an increase in protein yield. Robust protein expression can arise from modifications in the protein itself or the host cell it is expressed by (also called biological or genetic robustness), or a combination of both.

The term “non-recombinant protein” or “supplementary protein” is used herein to mean a protein that is not produced by a recombinant technology such as for example, inserting a heterologous gene in a host cell to have the host cell produce the heterologous amino acid sequence, peptide, protein or fragment thereof.

It must be noted that, as used in the specification and the appended claims, the singular forms “a,” “an” and “the” include plural referents unless the context clearly dictates otherwise.

6.2. Recombinant Protein Compositions

6.2.1. Animal Proteins

The disclosure provides various recombinant animal proteins for the inclusion into food for primarily humans and pets. It is contemplated that any recombinant animal protein can be used with the methods and compositions of the disclosure. Often the recombinant animal protein is a heterologous protein.

The recombinant animal protein used with the methods and compositions of the disclosure may be a full-length protein, a truncated protein, or a fragment of a protein. A fragment (or portion of a protein) is an amino acid sequence that has at least three amino acids of the full-length protein. In some embodiments, the full-length protein is produced by expressing fragments that cover the full-length protein.

In some embodiments, the amino acid sequence of the animal proteins may be modified by replacing one or more amino acids with a different amino acid (e.g., by changing the nucleotide sequence of the recombinant gene encoding the protein).

Such amino acid modifications may improve the yield of the animal protein (e.g., by more robust protein expression) produced by the host cell that has been engineered to express the protein. Any amino acid modification can be made that improves or enhances the production of the animal proteins. In some embodiments the modification is made in the protein encoding region of the animal protein. In other embodiments, the modification is made in a regulatory element that controls or modifies the expression of the animal protein. Non-limiting examples of such amino acid modifications are: improving the efficiency of transcription and/or translation of the animal protein, improving the stability of the animal protein, altering the rate at which the protein is secreted by the host cell or by changing the activity of the animal protein so any deleterious effects on the expression of the animal protein are minimized.

In some embodiments, the animal protein has a higher percentage of essential amino acids compared to other animal tissue proteins. In some embodiments, the animal protein comprises more than 1%, 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, 10%, 11%. 12%, 13%, 14%, 15%, 20%, 25%, 30%, 31%, 32%, 33%, 34%, 35%, 36%, 37%, 38%, 39% or 40% essential amino acids compared to other animal tissue proteins.

Depending on the host cell used, it may be helpful to select an animal protein that has a certain percentage of sequence identity to the proteins derived from the host cell. In some embodiments, the animal protein has 0%, 1%, 2%, 3%, 4%, 5%, 6%. 7%, 8%, 9%, 10%, 11%, 12%, 13%, 14%, 15%, 16%, 17%, 18%, 19%, 20%, 21%, 22%, 23%, 24%, 25%, 26%, 27%, 28%, 29%, 30%, 31%, 32%, 33%, 34%, 35%, 36%, 37%, 38%, 39%, 40%, 41%, 42%, 43%, 44%, 45%, 46%, 47%, 48%, 49%, or 50% sequence identity to a gene or a region of a gene derived from a host cell. In some embodiments the animal protein has 0%, 1%, 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, 10%, 11%, 12%, 13%, 14%, 15%, 16%, 17%, 18%, 19%, 20%, 21%, 22%, 23%, 24%, 25%, 26%, 27%, 28%, 29%, 30%, 31%, 32%, 33%, 34%, 35%, 36%, 37%, 38%, 39%, 40%, 41%, 42%, 43%, 44%, 45%, 46%, 47%, 48%, 49%, or 50% sequence identity to a protein or a fragment of a protein derived from a host cell.

Non-limiting examples of animal proteins that can be used with the disclosure are: troponin I, actin, myosin, alpha-actinin-2, alpha-actinin-3, titin, receptor tyrosine protein kinase skeletal muscle, myosin binding protein C, F-actin-capping protein, Myosin-binding protein H, troponin T, myotubularin 1, myozenin-1, beta-enolase, cofilin-2, PDZ and LIM domain protein 7, twinfilin-2, telethonin, M-protein striated muscle, coronin, nebulin-related-anchoring protein, myopalladin, tensin, gelsolin, dystroglycan, profilin, myozenin-2, calsarcin 1, myotilin, paxillin, integrin alpha-7, integrin beta-1, dystrophin, ankyrin, paranemin, myomesin (skelemin), alpha sarcoglycan, gamma sarcoglycan, or calponin.

Examples of animal muscle proteins (or relatives of those proteins) that can be used with the disclosure include but are not limited to: thymosin beta 4, metavinculin, parvalbumin beta, tripartite motif-containing protein 54, obscurin, muscle M-line assembly protein unc-89, muscle-type aldolase, SERCA1, calponin homology-associated smooth muscle protein, skeletal muscle ankyrin repeat protein, calpain-3, atrogin-1, striated muscle-specific serine/threonine-protein kinase, skeletal muscle LIM-protein 2, glycogen phosphorylase, serpin A3-1, cadherin, beta-taxilin, density-regulated protein, synaptopodin, ARP2/3, WASP, SCAR/WAVE, IQGAP, AbpI, cortactin, drebrin, ENA/VASP, annexin II, BPAG, ERM protein, Sla2, utrophin, Srv2/CAP, verprolin, formins, capZ, fragmin, villin, AIP1, adducin, MACF, MAP2, tau, fimbrin, scruin, espin, fascin, actinfilin, actinogelin, Arklp, Prklp, actobindin, actolinkin, alpha-parvin, actophorin, acumentin, scinderin, afadin, AFAP-110, affixin, aginactin, angiogenin, dystonin, anilin, archvillin, cortactin, caltropin, CARMIL, caerin-1.16, dematin, diaphanous, EF-1a, EF-1b, LIM domain and actin-binding protein, elongation factor 2, epsin, proheparin-binding EGF-like growth factor, Mitogen-activated protein kinase, frabin, four and a half LIM domains protein 3, FH1/FH2 domain-containing protein 3, GAS2-like protein 2, kettin, Kelch protein, limatin, PDZ and LIM domain protein 1, synaptopodin-2, prefoldin, presenilin I, receptor tyrosine-protein kinase erbB-2, protein kinase C, striated muscle-specific serine/threonine-protein kinase, rapsyn, shroom, smitin, smoothelin, or serine/threonine-protein phosphatase, laminin, sarcospan, dystrobrevin, syntrophin, dysbindin, dysferlin, or fukutin.

Preferred animal protein sequences are listed in Table 1. They are grouped according to the tissue in which they are highly expressed (known). If it is not known in what tissue a protein is expressed, the protein is grouped according to the tissue for which its expression is required (e.g., for normal development of the tissue). For example, it is known that myotubularin is required for normal skeletal muscle growth. Thus, it is grouped with the skeletal muscle proteins. Persons skilled in the art will appreciate that in some cases a protein can be expressed in one or more tissue types.

In certain embodiments, the food compositions described herein comprise one or more of the animal proteins set forth in Table 1. In related embodiments, the food compositions described herein additionally, or alternatively, comprise one more recombinantly expressed homologs of the animal proteins set forth in Table 1.

In other related embodiments, the food compositions described herein comprise one or more animal proteins that are at least 50%, 60%, 70%, 80%, 85%, 90%, or 95% identical, but less than 100% identical, to the proteins set forth in Table 1 (i.e., the protein sequences are modified to alter their amino acid content, e.g., to improve nutrition, to improve digestibility, to optimize expression or to optimize secretion).

In other related embodiments, the food compositions described herein comprise one or more animal skeletal muscle tissue proteins of Table 1, or one or more cardiac muscle tissue proteins of Table 1, or one or more smooth muscle tissue proteins of Table 1, or one or more of the skeletal/cardiac muscle tissue proteins of Table 1, or one or more of the skeletal/smooth muscle tissue proteins of Table 1, or one or more of the cardiac/smooth muscle tissue proteins of Table 1, or one or more of the skeletal/cardiac/smooth muscle tissue proteins of Table 1. In yet other related embodiments, the food compositions described herein comprise proteins from two or more of the above-mentioned categories of proteins described in Table 1.

In some embodiments, the animal protein is an actin cytoskeleton protein. In some embodiments, the actin cytoskeleton protein is a filament protein, a capping protein, an actin-binding protein, an actin-bundling protein, a monomer binding protein, a cytoskeletal linker protein, a membrane anchor protein, a stabilizing protein, a sidebinder protein, a signaling protein, a capping protein, a severing protein, or a myosin.

6.2.2. Nucleic Acids Encoding the Animal Proteins

Production of a recombinant animal protein of the disclosure can be achieved by the manipulation of a gene that encodes an animal protein, which is then inserted in a host cell expression system such that it expresses large amounts of a recombinant gene that is converted into an animal protein using the host cell expression system. This process can include the transcription of the recombinant DNA to messenger RNA (mRNA), the translation of mRNA into polypeptide chains, which are ultimately folded into functional proteins and may be targeted to specific subcellular or extracellular locations depending on the sequence. However, an animal protein need not be folded or targeted to add to the nutritional value of a food product. Where the animal protein is a fragment or portion of an animal protein it may not be folded.

Genes encoding recombinant animal proteins can be obtained by taking a sample from an animal and extracting nucleic acids, such as mRNA, from that sample and then amplifying the gene by reverse transcription followed by PCR. The sample could be a tissue sample (e.g., muscle), a blood sample, mucus, skin, saliva, or hair. Another option is to have the gene synthesized by a company that performs such work.

Alternatively, where the genome sequence of the animal has been determined, the gene sequences (DNA/nucleotide sequences) or protein sequences of an animal can be obtained by searching appropriate databases (e.g., UniProtKB and NCBI). A polynucleotide can be obtained using chemical synthesis, molecular cloning or recombinant methods, DNA or gene assembly methods, artificial gene synthesis, PCR, or any combination of those.

In the case that there are not sequences available for an animal protein of interest, conserved regions can be used to amplify segments of the genes and the flanking regions can be sequenced in order to obtain the full-length sequence. Multiple sequence alignments of a specific protein in several different organisms will show where the conserved regions lie, and which are the most suitable stretches to use for primer design. Primers with alternative nucleotides can be used when needed.

The present invention provides codon-optimized nucleic acid encoding an animal protein for expression in a host cell. Codon-optimization for expression in a particular host cell can be determined by codon usage tables or by using a program that is instructed by an algorithm that identifies a region of sequence that can be optimized for protein expression in the host cell. Any commercially available optimization algorithm or any publicly available algorithms can be used with the disclosure. Using such programs, various improvements can be achieved to enhance expression of a recombinant animal protein as discussed herein. Specific examples of codon-optimization of animal protein gene sequences for certain host cells are provided herein.

The gene sequences that can be used with the methods and compositions of the disclosure are those encoding the types of proteins described herein. In some embodiments, the gene sequence may include non-coding introns. In some embodiments, the gene sequences may not include non-coding introns.

Depending on what method is used to produce a recombinant animal protein, a gene encoding the animal protein may further comprises one or more regulatory elements. Non-limiting examples of regulatory elements include but are not limited to such as a restriction enzyme site, a promoter, an enhancer, a signal sequence, a terminator, or a combination thereof

6.2.2.1. Origin

The identification and cloning of an animal protein are discussed herein. The origin of the recombinantly expressed protein sequence (i.e., the species of animal from which the sequence to be recombinantly expressed is found in nature) can be any species within the biological kingdom of Animalia. Preferably, the origin of the recombinantly expressed protein sequence is a vertebrate animal, which can be a fish, a bird, a mammal, an amphibian, or a reptile. The origin may be a placental mammal, monotreme mammal, or marsupial mammal (metatheria). The origin may furthermore be a bird or another vertebrate from the reptile clade.

In some embodiments, the gene origin is a placental mammal, including but not limited to carnivores (including lion, bear, weasel, seal, wolf, coyote, fox), equidae (including horse and donkey), even-toed ungulates (including pig, camel, cattle, and deer), Afrotheria (including elephants, woolly mammoth, golden moles, and manatees), and Boreoeutheria (including primates, rabbits, hares, pikas, rodents, moles, whales, bats, dogs, cats, seals, and hoofed mammals).

In some embodiments, the origin is a monotreme mammal, including but not limited to platypus and echidna. In some embodiments, the origin is a marsupial mammal, including but not limited to koala, possums, tapirs, kangaroos, wallabies, and marsupial lions.

In some embodiments, the origin is a hoofed mammal, including but not limited to cattle, antelope, deer, reindeer, elk, sheep, goat, camels, carabao, yak, bison, buffalo, caribou, water buffalo, pig, horse, and donkey. In some embodiments, the origin is an endothermic vertebrate, classified as Ayes, including but not limited to chicken, turkey, duck, pigeon, penguin, ostrich, goose, pheasant, and quail.

In some embodiments, the gene origin is a reptile, including but not limited to alligators and crocodiles.

In some embodiments, the gene origin is an aquatic animal, including but not limited to shark, tuna, trout, salmon, herring, jacks, carp, catfish, cod, flounder, bass, tilapia, sturgeon, crab, lobster, shrimp, prawns, oysters, mussels, eels, shellfish, cuttlefish, starfish, crayfish, and jellyfish.

In some embodiments, the gene origin is an amphibian, including but not limited to frogs, salamanders, and toads. In some embodiments, the gene origin is an insect.

6.2.2.2. Tissue Source

The recombinant animal protein may be from any organ or tissue of an animal, including, but not limited to proteins expressed in the brain, skin, scales, feathers, eyes, shells, hair, horns, ears, liver, heart, kidney, stomach, intestines, and muscle tissue (e.g., skeletal, smooth or cardiac).

In preferred embodiments, the recombinant animal proteins are muscle proteins. In some embodiments, the recombinant animal protein is cytoskeletal. In some embodiments, the actin cytoskeleton protein is a filament protein, a capping protein, an actin-binding protein, an actin-bundling protein, a monomer binding protein, a cytoskeletal linker protein, a membrane anchor protein, a stabilizing protein, a sidebinder protein, a signaling protein, a capping protein, a severing protein, or a myosin. In some embodiments, the recombinant animal protein is a myosin. In some embodiments, the recombinant animal protein is an actin.

The animal muscle proteins include those proteins normally found in animal muscle tissue (or relatives of those proteins). In addition to myosin and actin, these proteins include but are not limited to troponin, tropomyosin, alpha-actinin, beta-actinin, titin, connectin, skeletal receptor, myosin-binding protein, desmin, leiomodin, tubulin, myotubularin, myozenin, telethonin, calsarcin, myotilin, nebulin, nebulin-related anchoring protein, myomesin, vinculin, paxillin, beta-enolase, myotubularin, calponin, caldesmon, transgelin, tropomodulin, supervillin, gelsolin, twinfilin, profilin, caveolin, catenin, cofilin, capping protein, leiomodin, tensin, M-protein, radixin, filamin, keratin, myopalladin, calsequestrin, caveolae-associated protein, nebulette, coronin, talin, dystrophin, dystroglycan, integrin, ankyrin, syncoilin, smoothelin-like-1, spectrin, synemin, paranemin, ponsin, plectin, skelemin, sarcoglycan, LIM protein, myoblast determination protein, myocyte-specific enhancer, and myocilin.

6.2.3. Expression Vectors

The disclosure also provides various expression vectors (e.g., constructs) comprising a genetic element (e.g., DNA, or cDNA) encoding for a protein derived from an animal. Depending on the host cell used for protein expression, a person skilled in the art of biotechnology will know the appropriate expression vector to use (e.g., plasmid, virus) with the regulatory elements (e.g., transcriptional start site, promoter, and the like) and genetic elements required for protein expression in a particular host cell. Specific examples of expression vectors that can be used with the disclosure are provided herein.

A genetic element is any coding or non-coding nucleic acid sequence. A genetic element can be a nucleic acid that codes for an amino acid, a peptide or a protein. Genetic elements may be operons, genes, gene fragments, promoters, exons, introns, regulatory sequences, or any combination of those. A genetic element includes an entire open reading frame of a protein, or the entire open reading frame and one or more (or all) regulatory sequences associated therewith. The genes may be codon-optimized for expression in a particular recombinant host cell (e.g., codon-optimized for yeast, insect, or mammalian host cell).

In some embodiments, an expression vector can comprise one genetic element. In some embodiments, an expression vector can comprise at least 2, 3, 4, 5, or 6 genetic elements. In some embodiments, an expression vector can comprise one regulatory element. In some embodiments, an expression vector can comprise at least 2, 3, 4, 5, or 6 regulatory elements. A person skilled in the art knows the payload limitations (e.g. kilobase pairs) for certain various expression vectors (e.g., cosmids, plasmids, etc).

The term “engineered” or “recombinant” refers to a cell into which a recombinant gene, such as, for example, a gene encoding an animal protein, or part of an animal protein, has been introduced. Therefore, engineered cells are distinguishable from naturally occurring cells that do not contain a recombinant gene that is introduced by transfection, transformation, cell fusion, mating or other techniques. Recombinantly introduced genes will either be in the form of a cDNA (i.e., they will not contain introns), a copy of a cDNA gene, genomic DNA (with or without introns; for expression in prokaryotic hosts, the DNA should be without introns), or will include DNA sequences positioned next to a promoter not naturally associated with the particularly introduced gene.

6.2.3.1. Promoters

Disclosed herein are expression vectors comprising a genetic element encoding an animal protein or part of an animal protein and the use thereof for the recombinant expression of the animal protein.

The expression vector may further comprise a promoter. The promoter may be a constitutive promoter, an inducible promoter, or a hybrid promoter. Where overexpression of a protein is toxic to a host cell (e.g., reduces growth of the cell, kills the cell, or reduces protein expression) it may be preferable to use an inducible promoter.

In the expression vector, the gene construct and the method, the promoter may be a viral promoter, a prokaryotic promoter or a eukaryotic promoter. The promoter may be a synthetic promoter from a promoter library. The promoter may be any scientifically known promoter or a novel promoter. The promoter may be an engineered form of a known promoter or a hybrid promoter.

The eukaryotic promoter may be a fungi promoter, a plant promoter, or an animal promoter. The fungi promoter may be the promoter of the genes phosphoglycerate kinase (PGK, PGK1, PGK3), enolase (ENO, ENOl), glyceraldehyde-3-phosphate dehydrogenase (gpdA, GAP, GAPDH), hexokinase, pyruvate decarboxylase, phosphofructokinase, glucose-6-phosphate isomerase, 3-phosphoglycerate mutase, pyruvate kinase, triosephosphate isomerase, phosphoglucose isomerase, glucokinase, alcohol dehydrogenase promoter (ADH1, ADH2, ADH4), isocytochrome C, acidic phosphatase, galactose metabolism enzymes, GAL (GAL1, GAL2, GAL3, GAL4, GAL5, GAL6, GAL7, GAL8, GAL9, GAL10), alternative oxidase (AOD), alcohol oxidase 1 (AOX1)), alcohol oxidase 2 (AOX2), CUP1, AHSB4m, adhl+, AINV, alcA, AXDH, cellobiohydrolase I (cbhl), ccg-1, cDNAl, cellular filament polypeptide (cfp), cpc-2, ctr4+, dihydroxyacetone synthase (DAS), FMD, formate dehydrogenase (FMDH), formaldehyde dehydrogenase (FLD1), GAA, GCW14, glucoamylase (glaA, gla-1), invl, isocitrate lyase (ICL1), glycerol kinase (GUT1), acetohydroxy acid isomeroreductase (ILV5), β-galactosidase (lac4), LEU2, melO, MET3, MET25, KAR2, KEX2, methanol oxidase (MOX), nmtl, peroxin 8 (PEX8), pcbC, PET9, PH05, PH089, PYK1, phosphatidylinositol synthase (PIS1), RPS7, TEF, translation elongation factor 1 alpha (TEF1), sorbitol dehydrogenase (SDH), SSA4, THI11, homoserine kinase, XRP2, TPI, and YPT1, PHOS, CYC1, HIS3, ADC1, TAP1, URA3, LEU2, TP1, TDH1, TDH3, FBA1, ADR1, TPI1, or any combination of those.

The plant promoter may be the promoter of the gene phol, TPI, TPS1, and any combination of these.

The animal promoter may be a heat-shock protein promoter, proactin promoter, immunoglobulin promoter, or the promoter of the gene B2, HSP82, Ser1, triose phosphate isomerase (TPI1), or any combination of those. However, any promoters can be used if they drive the expression of recombinant proteins in a particular host cell.

6.2.3.2. Selection Gene Marker

The expression vector may include a selection gene marker. For example, an expression vector may comprise an auxotrophic marker. Non-limiting examples of auxotrophic markers that can be used with the disclosure include trp1, leu2, his3, adel, arg4, his4, ura3, and/or met2. In some embodiments, more than one selection gene marker may be used.

In some embodiments, the expression vector may comprise a selectable marker, which may be an antibiotic resistance gene. The resistance gene may confer resistance to drugs including, but not limited to, zeocin, ampicillin, blasticidin, kanamycin, nurseothricin, chloroamphenicol, tetracycline, triclosan, or ganciclovir. In some embodiments, more than one resistance genes may be used. Yet, for some food compositions, it may be desirable not to use an antibiotic resistance gene for selection.

In applications when there are several animal proteins expressed, it may be useful to use one or more resistance genes in combination with one or more auxotrophic markers.

6.2.3.3. Integration and Transformation

The compositions of the invention include a recombinant host cell transformed with an expression vector to express one or more recombinant animal proteins.

One or more expression vectors with the required genetic elements (e.g., regulatory elements or protein-encoding, genetic elements) may be integrated into a genome. In some applications, it may be desirable to integrate multiple copies of the same expression vector.

Alternatively, or in addition, the host cell may comprise multiple copies of an expression vector where the expression vector is not integrated into a genome.

Any small DNA molecule within a cell that is capable of being physically separated from chromosomal DNA and can replicate can be used with the methods and compositions of the disclosure. The expression vectors that can be used with the disclosure are a plasmid, a conjugative plasmid, a non-conjugative plasmid, a cosmid, a hybrid plasmid, a virus, a phage, or the like.

Host cells may be transformed or transduced to introduce the expression vector by transfection, infection, endocytosis, F-mating, mating, PEG-mediated protoplast fusion, Agrobacterium tumefaciens-mediated transformation, chemical transformation, electroporation, heat-shock transformation, biolistic transformation or any other method known in the art.

6.2.3.4. Signal Peptide Sequence

The expression vector may further comprise a signal peptide sequence. A signal peptide, also known as a, signal sequence, targeting signal, localization signal, localization sequence, secretion signal, transit peptide, leader sequence, or leader peptide, may cause extracellular secretion of a protein.

Extracellular secretion of a recombinant animal protein from a host cell simplifies protein purification. Recovery of a recombinant animal protein from a cell culture supernatant may be preferable to lysing host cells to release a complex mixture of proteins including intracellular proteins of the host cell.

For some applications, secretion may reduce harmful effects that intracellular overexpression of a recombinant animal protein may have on a host cell such as toxicity or reduced growth rate.

Secretion may produce higher amounts of an animal protein compared to intracellular expression. Secretion of a protein may also enable post-translational modification (e.g., glycosylations) or aid in folding the protein correctly and allow for the formation of disulfide bonds.

6.2.4. Host Cells

The expression vectors provided by the disclosure are transformed into host cells. Typically, the host cell is a eukaryotic host cell.

Any eukaryotic host cell known in the art can be used with the expression vectors and animal proteins provided by the disclosure to make a recombinant host cell. Examples of a eukaryotic host cell that can be used with the disclosure are an insect cell, a fungal cell, a plant cell, and a mammalian cell.

Genetic modification of the host cell is accomplished in one or more steps via the design and construction of appropriate vectors and transformation of the host cell with those vectors. Electroporation and/or chemical (such as calcium chloride- or lithium acetate-based) transformation methods can be used. Methods for transforming yeast strains are described in WO 99/14335, WO 00/71738, WO 02/42471, WO 03/102201, WO 03/102152 and WO 03/049525; these methods are generally applicable for transforming host cells in accordance with this invention. The DNA used in the transformations can either be cut with particular restriction enzymes or used as circular DNA.

The recombinant host cells can be cultured in appropriate media to produce large quantities of the recombinant animal protein.

6.2.4.1. Yeast Host Cells

In some embodiments, the host cell used to express the protein is a yeast host cell. The yeast cell can be a budding yeast, fission yeast, or a filamentous yeast. In some applications, the yeast host cell is a wild-type yeast. However, often, the yeast host cell used with the method and compositions of the disclosure is a modified yeast host cell (e.g., through mutation, genome shuffling, protoplast fusion, cytoduction, etc.) to enhance the production or yield of protein, aid selection of, or any other modification that enhances production of the animal protein such that host cell gives more robust expression (i.e., strain robustness). The modification can result in a yeast host cell that is polyploid or aneuploid. In some applications, the host cell may be modified so that it grows faster, grows to a higher cell density, is less sensitive to environmental factors in the bioproduction process fluctuations such an unexpected change in temperature or reduced nutrients.

The yeast host cell may be obtained from a variety of sources known to people skilled in the art, including commercial sources. In some embodiments, the yeast host cell may be selected from the “Saccharomyces Yeast Clade”, as described in US Publication No. 2009/0226991.

In certain embodiments, the yeast host cell is a Saccharomyces sensu stricto yeast. The term “Saccharomyces sensu stricto” taxonomy group is a cluster of yeast species that are highly related to S. cerevisiae (Rainieri et al., 2003, J. Biosci Bioengin 96: 1-9). Saccharomyces sensu stricto yeast species include but are not limited to S. cerevisiae, S. kudriavzevii, S. mikatae, S. bayanus, S. uvarum, S. carocanis and hybrids derived from these species (Masneuf et al., 1998, Yeast 7: 61-72).

An ancient whole genome duplication (WGD) event occurred during the evolution of the hemiascomycete yeast and was discovered using comparative genomic tools (Kellis et al., 2004, Nature 428: 617-24; Dujon et al., 2004, Nature 430:35-44; Langkjaer et al., 2003, Nature 428: 848-52; Wolfe et al., 1997, Nature 387: 708-13). Using this major evolutionary event, yeast can be divided into species that diverged from a common ancestor following the WGD event (termed “post-WGD yeast” herein) and species that diverged from the yeast lineage prior to the WGD event (termed “pre-WGD yeast” herein).

In some embodiments, the yeast host cell may be selected from a post-WGD yeast genus, including but not limited to Saccharomyces and Candida. In some embodiments, post-WGD yeast species include: S. cerevisiae, S. uvarum, S. bayanus, S. paradoxus, S. castelli, and C. glabrata.

In some embodiments, the yeast host cell may be selected from a pre-whole genome duplication (pre-WGD) yeast genus including but not limited to Saccharomyces, Kluyveromyces, Candida, Pichia, Issatchenkia, Debaryomyces, Hansenula, Yarrowia and, Schizosaccharomyces. Representative pre-WGD yeast species include: S. kluyveri, K thermotolerans, K. marxianus, K. waltii, K. lactis, C. tropicalis, P. pastoris, P. anomala, P. stipitis, I. orientalis, I. occidentalis, I. scutulata, D. hansenii, H anomala, Y. lipolytica, and S. pombe.

A yeast host cell used with the disclosure may be either Crabtree-negative or Crabtree-positive, as described in US Publication No. 2009/0226991. A yeast microorganism may be either Crabtree-negative or Crabtree-positive. A yeast cell having a Crabtree-negative phenotype is any yeast cell that does not exhibit the Crabtree effect. The term “Crabtree-negative” refers to both naturally occurring and genetically modified organisms. Briefly, the Crabtree effect is defined as the inhibition of oxygen consumption by a microorganism when cultured under aerobic conditions due to the presence of a high concentration of glucose (e.g., 50 g glucose L⁻¹). In other words, a yeast cell having a Crabtree-positive phenotype continues to ferment irrespective of oxygen availability due to the presence of glucose, while a yeast cell having a Crabtree-negative phenotype does not exhibit glucose mediated inhibition of oxygen consumption.

In some embodiments, the yeast host cell may be selected from yeast with a Crabtree-negative phenotype including but not limited to the following genera: Saccharomyces, Lachancea, Kluyveromyces, Pichia, Issatchenkia, Komagataella, Yarrowia, Hansenula, Debaromyces, Ogataea, Zygosaccharomyces and Candida. Crabtree-negative species include but are not limited to: L. kluyveri (fka S. kluyveri), K. lactis, K. marxianus, P. anomala, S. stipitis (fka P. stipitis), I. orientalis, D. occidentalis, P. scutulata, P. anomala, Ogataea polymorpha, Arxula adeninivorans, Cyberlindnera jadinii, K. phaffii, Y. lipolytica, Kluyveromyces fragilis, D. hansenii, P. kudriavzevii and C. utilis.

In some other embodiments, the yeast host cell may be selected from yeast with a Crabtree-positive phenotype, including but not limited to the genera Saccharomyces, Kluyveromyces, Zygosaccharomyces, Naumovozyma, Lachancea, Dekkera, Candida, Pichia and Schizosaccharomyces. Crabtree-positive yeast species include but are not limited to: S. cerevisiae, S. uvarum, S. bayanus, S. paradoxus, N. castellii, L. thermotolerans, C. glabrata, Z. bailii, Z. rouxii, D. bruxellensis and S. pombe.

Another characteristic may include the property that the host cell is non-fermenting. In other words, it cannot metabolize a carbon source anaerobically while the yeast is able to metabolize a carbon source in the presence of oxygen. Nonfermenting yeast refers to both naturally occurring yeasts as well as genetically modified yeast.

In some embodiments, the recombinant host cells may be host cells that are non-fermenting yeast host cells, including, but not limited to those classified into a genus selected from the group consisting of Tricosporon, Rhodotorula, Myxozyma, or Candida. In a specific embodiment, the non-fermenting yeast is C. xestobii.

6.2.4.2. Mammalian Host Cells

Cultured mammalian cell lines may also be used to express the animal proteins provided by the disclosure. In some embodiments, Chinese hamster ovary (CHO) can be used. In some embodiments, human cell lines such as HEK or HeLa may be used to produce protein. In some embodiments, a commercially available mammalian expression system can be used such Expi293, ExpiCHO, ExpiCHO, T-REx Expression System, Flp-In T-REx system, GeneSwitch System from Thermofisher.

6.3. Methods for Bioproduction

6.3.1. Cell Culture Processes and Fermentation

The bioproduction of a recombinant animal protein may be conducted by cell culture processes or by fermentation. When fermentation is used, it may be conducted aerobically, microaerobically or anaerobically.

In some embodiments, the method for producing a recombinant animal protein for a food product consumption comprises (i) providing a reactor or flask comprising a fungal colony and (ii) a feedstock comprising a nitrogen-containing material and a carbon-containing material (e.g., sugar), and permitting the fungal colony to grow in presence of the feedstock to yield the fungus-containing product comprising a recombinant animal protein. In some embodiments, a selective media or reagent can be used to select for host cells harboring the recombinant animal gene.

In some embodiments, the method for producing a recombinant animal protein for a food product consumption comprises (i) providing a reactor comprising a fungal colony and (ii) a feedstock comprising a nitrogen-containing material and a sugar-containing material, and (iii) when the fungal colony reaches the exponential growth phase and an inducing agent is added to yield the fungus-containing product comprising a recombinant animal protein.

In some embodiments, the fungal colony comprises one or more budding fungi. Examples of preferred budding fungi are Saccharomyces cerevisiae, Schizosaccharomyces pombe, Komagataella phaffii, Kluyveromyces lactis, and a derivative thereof.

In some embodiments, the genome of a budding fungi can be genetically modified in at least one gene to yield more robust protein expression. Genetic modifications that can yield more robust protein expression are discussed herein. In some embodiments, the genome of a budding fungi can be genetically modified to be protease deficient.

In some embodiments, the fungal colony does comprise one or more filamentous fungi. Non-limiting examples of filamentous fungi that can be used are Aspergillus oryzae, Trichoderma reesei, Fusarium venenatum, Geotrichum candidum, Penicillium camemberti, Penicillium roqueforti, and a derivative thereof.

In some embodiments, the genome of a filamentous fungi can be genetically modified in at least one gene to yield more robust protein expression. Genetic modifications that can yield more robust protein expression are discussed herein. In some embodiments, the genome of a filamentous fungi can be genetically modified to be protease deficient.

In some embodiments, the recombinant animal protein is produced in a recombinant host cell and expressing the recombinant animal protein intracellularly. In some embodiments, the recombinant animal protein is produced in a recombinant host cell and expressing the recombinant animal protein such that it is secreted into the culture broth.

The recombinant animal protein may be obtained by a whole-cell preparation (i.e., host cell itself, and the recombinant protein expressed within or on its surface, can be added to the food composition), a protein concentrate preparation, or by isolating an animal protein. Depending on where the protein is expressed in the cell (e.g., extracellularly or intracellularly) protein concentrate can be from a cell lysate or a cell supernatant after centrifugation.

6.3.2. Harvesting of Intermediate Food Product

The disclosure also provides methods for making an intermediate food product.

In some embodiments, the method comprises culturing eukaryotic host cell that recombinantly expresses a heterologous animal protein and harvesting the recombinant host cell, thereby making an intermediate food product.

In some embodiments, the method comprises culturing eukaryotic host cell that recombinantly expresses an animal protein, concentrating the recombinant host cell from the culture, extracting proteins in a protein concentrate from the concentrated culture, thereby making an intermediate food product.

In some embodiments, the method comprises culturing eukaryotic host cell that recombinantly expresses an animal protein, concentrating the recombinant host cell from the culture, and isolating the animal protein, thereby making an intermediate food product.

Where the animal protein is expressed intracellularly in the host cell, a cell lysate can be obtained from the eukaryotic host cell to make the intermediate food product. Where the animal protein is expressed extracellularly, the cell supernatant can be obtained the intermediate food product.

The intermediate food product can also be made in a format such that it is used to another food product. In some embodiments, the intermediate food product is harvested and made in the format an ingredient, a coating, a palatability agent, or a flavoring agent as discussed in more detail below.

6.4. Intermediate Food Products

The disclosure also provides various intermediary food products comprising the recombinant animal protein. The intermediary food product can be substantially free of an antibiotic, an animal growth hormone, animal meat, or proteins derived from animal meat.

The recombinant animal protein can be harvested and provided to the intermediary food product as a whole-cell food composition, a protein concentrates food composition, or as a protein isolate food composition. An intermediate food product can be mixed, coated, soaked or injected into an ultimate ingestible food product. The ultimate ingestible food product can be a commercially available feed, food, supplement, or treat.

In some embodiments, the intermediary food is a wet or dry ingredient that is added to another food product. The intermediary food can also be a coating to be added to the exterior of a food product. The coating can be soaked, brushed, or sprayed on a food product. In some embodiment the intermediary food protein can be a palatability agent that enhances the acceptance of the food product, as a flavoring agent or agent that enhances mouth-feel (e.g., texture and the like).

In some embodiments, the harvested whole cell, protein concentrate, or protein isolate can be concentrated and dried, thereby making a dry intermediate food product. A dry intermediate food product comprising the recombinant animal protein can be in the form of a powder, a granule, a pellet, a slurry or paste, of varying moisture content.

6.5. Food Product Compositions

The disclosure provides various food product compositions (for humans and pets) comprising the recombinant animal protein as well as supplements. The food product can be substantially free of an antibiotic, an animal growth hormone, animal meat, or proteins derived from animal meat. In some embodiments, the food product is substantially free of any other ingredient. In other embodiments, the food product is combined with other ingredients.

The recombinant animal protein-containing food product can be formulated as a primary diet food product for an animal or individual (e.g. that is, it acts as the core source of daily nutrition). Examples of a primary food product include but are not limited to a meal, a kibble, a wet food, a dry food (e.g., freeze-dried or dehydrated).

The recombinant animal protein-containing food product can be formulated as secondary diet food product (that is, it does not provide nutrients in the amounts that are required for daily nutrition for an animal or individual). Examples of a secondary diet food products are a snack, a treat, or an edible toy.

The recombinant animal protein-containing food product can also be made from an intermediary diet food product (e.g., ingredient, a coating, a palatability agent, or a flavoring agent) that is added to make an ultimate ingestible food product.

6.5.1. Dry and Wet Food Products

The recombinant animal protein is introduced into a dry or wet food composition by addition of the intermediate food product, which can be a whole-cell food product, a protein concentrate food product, or as a protein isolate food product, thereby making a dry food product. In some embodiments, the dry food product can be further processed and shaped into a kibble, a treat, a snack, a chew, or an edible toy.

In some other embodiments, intermediate food product, which can be a whole cell, protein concentrate, or protein isolate can be concentrated, dried, and then rehydrated with one or more wet ingredients thereby making a wet food product. Wet products comprising the recombinant animal protein can be in the form of a slurry, a paste, a suspension, or a liquid. The wet food composition maybe semi-moist, intermediate moist, or moist. In some embodiments, the wet food composition can be further processed and shaped into a kibble, a treat, a snack, a chew, or a toy.

Depending on the percentage of essential amino acids desired for a food composition one can determine the amount of intermediate food product needed to achieve the desired amino acid content in the final food product (e.g. dry or wet food product). For example, the contribution of amino acids from profilin can be calculated for different expression levels (Table 4).

To carry out this example calculation, it was assumed that the total protein content per dry cell weight was constant. It was also assumed that profilin expression did not change the profile of endogenous cellular proteins, and that expression of endogenous proteins decreased (in percent) the same as profilin increased (on a mass basis). The current estimated expression level of profilin is 10% of the total protein. The level was calculated based on the intensity of the protein bands in FIG. 5 using the software Image J (Schneider, Rasband, & Eliceiri, 2012; NIH Image to ImageJ: 25 years of image analysis. Nature Methods, 9, 671-675).

TABLE 4 Increase in essential amino acids at different levels of profilin expression Profilin expression level (wt % of total protein) 2% 4% 6% 8% 10% 12% 15% 20% 30% 40% 50% Increase in amino acid content per dry weight Arginine 0.23% 0.47% 0.70% 0.93% 1.17% 1.40% 1.75% 2.34% 3.51% 4.67% 5.84% Histidine −0.66% −1.33% −1.99% −2.66% −3.32% −3.99% −4.98% −6.64% −9.96% −13.3% −16.6% Isoleucine 0.39% 0.79% 1.18% 1.57% 1.97% 2.36% 2.95% 3.93% 5.90% 7.86% 9.83% Leucine −0.17% −0.34% −0.52% −0.69% −0.86% −1.03% −1.29% −1.72% −2.58% −3.44% −4.30% Lysine 0.15% 0.30% 0.44% 0.59% 0.74% 0.89% 1.11% 1.48% 2.22% 2.96% 3.70% Methionine 3.22% 6.44% 9.66% 12.9% 16.1% 19.3% 24.1% 32.2% 48.3% 64.4% 80.5% Phenylalanine 0.42% 0.83% 1.25% 1.66% 2.08% 2.49% 3.12% 4.16% 6.23% 8.31% 10.4% Threonine 0.97% 1.94% 2.92% 3.89% 4.86% 5.83% 7.29% 9.72% 14.6% 19.4% 24.3% Tryptophan 1.49% 2.99% 4.48% 5.97% 7.46% 8.96% 11.2% 14.9% 22.4% 29.9% 37.3% Valine 0.75% 1.50% 2.26% 3.01% 3.76% 4.51% 5.64% 7.52% 11.3% 15.0% 18.8%

6.5.1.1. Whole-Cell Food Products

The disclosure also provides a whole-cell food product compositions. The whole-cell food product composition is made with the host cell expressing the recombinant animal protein.

Host cells expressing recombinant animal protein may be harvested by batch centrifugation, continuous flow centrifugation, filter press, flocculation, rotary drum vacuum filtration, tangential flow filtration, ultrafiltration or combination of these methods or any technique known in the art.

Cells may be lysed by raising temperature, autolysis, by high-pressure homogenization (e.g., French press), ultrasonic cavitation, bead beating, rotor-stator processors, freeze-thaw cycles, enzymatic lysis (e.g., lysozyme, lysostaphin, zymolase, cellulose, protease or glycanase), osmotic shock methods, chemical lysis (by alkaline, detergent or organic solvent) or a combination of these methods or any technique known in the art.

In some embodiments, food product comprising the recombinant animal protein is a whole-cell food product. In some embodiments, the whole-cell food composition comprises about 1%, 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, 10%, 15%, 20%, 25%, 30%, 35%, 40%, 45%, 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, or 90% of recombinant animal protein by dry weight, semi-moist weight, or wet weight.

6.5.1.2. Protein Concentrate Food Products

The disclosure also provides protein concentrate food product compositions. In some embodiments, the protein concentrate food product comprising the recombinant animal protein is made from a protein concentrate from a host cell expressing the recombinant animal protein.

Depending on whether the animal protein is expressed intracellularly or extracellularly in the host cell, the animal protein can be harvested from a cell lysate or cell supernatant of the host cell, respectively.

A protein concentrate can be purified from a host cell lysate or host cell supernatant by any technique known in the art.

In some embodiments, the protein isolate food composition comprises about 3%, 4%, 5%, 6%, 7%, 8%, 9%, 10%, 15%, 20%, 25%, 30%, 35%, 40%, 45%, 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, or 90% of recombinant animal protein by dry weight, semi-moist weight, or wet weight.

6.5.1.3. Protein Isolate Food Products

The disclosure also provides protein isolate food product compositions. In some embodiments, the protein isolate food product comprising the recombinant animal protein is made from a protein isolate from a host cell expressing the recombinant animal protein. Where a protein isolate is desired the gene encoding the animal protein will often further comprise a molecule tag or label that can facilitate the isolation of the animal protein. In some embodiments, one or more tags or labels can be used to isolate different animal proteins expressed in the same host cell.

Depending on if the animal protein is expressed intracellularly or extracellularly in the host cell, the animal protein can be harvested from a cell lysate or cell supernatant of the host cell, respectively. The animal proteins can be isolated using techniques known in the art.

In some embodiments, the protein isolate food composition comprises about 3%, 4%, 5%, 6%, 7%, 8%, 9%, 10%, 15%, 20%, 25%, 30%, 35%, 40%, 45%, 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, or 90% of recombinant animal protein by dry weight, semi-moist weight, or wet weight.

6.5.2. Other Ingredients

A recombinant animal protein of the disclosure may be combined with other ingredients such as fats, carbohydrates, supplemental non-recombinant proteins, fiber, nutritional supplements (e.g., minerals, and vitamins) to make a food composition.

In some embodiments, the recombinant animal protein of the disclosure may be combined with other ingredients to make a food product that meets the nutritional requirements of an animal (i.e., a nutritionally balanced food product). In some embodiments, the recombinant animal protein of the disclosure may be combined with other ingredients to make a food product more palatable to an animal or an individual.

In some embodiments, the recombinant animal protein of the disclosure may be combined with other ingredients to meet the nutritional requirements of an animal and to make it more palatable to an animal or an individual.

6.5.2.1. Amino Acids

For some food compositions, such as a primary diet food, it may be desirable to combine the recombinant animal protein with additional amino acids. Any amino acid that makes a food composition nutritionally balanced for an animal can be added to a food composition of the disclosure. Examples of amino acids that can be added to a food composition of the disclosure include but are not limited to Arginine, Histidine, Isoleucine, Leucine, Lysine, Methionine, Methionine/Cystine, Phenylalanine, Phenylalanine/Tyrosine, Threonine, Tryptophan, and Valine.

6.5.2.2. Fat and Carbohydrates

For some food compositions, it may be desirable to combine the recombinant animal protein with fat and/or carbohydrates.

Fat and carbohydrates are obtained from a variety of sources including but not limited to animal fat, fish oil, vegetable oil, meat, meat by-products, grains, other animal or plant sources, or any combination thereof.

In some embodiments, the food product can comprise omega-3 polyunsaturated fatty acids such as docosahexaenoic acid (“DHA”) or eicosapentaenoic acid (“EPA”) or a mixture of DHA and EPA.

Grains include but are not limited to rice, wheat, corn, barley, buckwheat, sorghum, oats, and quinoa. Other plant sources include but are not limited to pulses (chickpeas and different beans) and edible roots (e.g., potato, sweet potato, carrot, cassava, and turnips).

6.5.2.3. Non-Recombinant Proteins

For some food compositions, it may be desirable to combine the recombinant animal protein with additional proteins (i.e., also referred to as “supplementary proteins” or “non-recombinant proteins”).

Such, supplementary proteins or non-recombinant proteins, can be obtained from a variety of sources including plants, animals, or microbes (unicellular and multicellular).

Supplemental proteins may also be obtained from an animal, which includes meat, meat by-products, dairy, and eggs. Meats include the flesh from poultry, fish, and animals such as cattle, swine, sheep, goats, deer, and the like. Meat by-products include but are not limited to kidneys, lungs, livers, stomachs, and intestines.

In some embodiments, the supplementary proteins may be free amino acids and/or peptides.

6.5.2.4. Fiber

For some food compositions, it may be desirable to combine the recombinant animal protein with fiber. Fiber can be obtained from a variety of sources such as vegetable fiber sources, including but not limited to beans, cellulose, beet pulp, parsnips, broccoli, peanut hulls, carrots, spinach, and soy fiber.

6.5.2.5. Nutritional Supplements

For some food compositions, it may be desirable to combine the recombinant animal protein with nutritional supplements. The nutritional supplement can be an antioxidant, a vitamin, a mineral, or a nutrient.

The nutritional supplements may be obtained from a variety of sources known to people skilled in the art including commercial sources. Vitamins and minerals can be added to a food product in amounts required to avoid deficiency and maintain health.

Non-limiting examples of nutrients that can be used with the disclosure include but are not limited to choline, thiamine, egg powder, manganese, methionine, cysteine, L-carnitine, lysine, and mixtures thereof.

Non-limiting examples of antioxidants include but are not limited to vitamin E, vitamin C, taurine, beta-carotene, and mixtures thereof.

Vitamins generally useful as food additives include vitamin A, vitamin B1, vitamin B2, vitamin B6, vitamin B12, vitamin D, vitamin E, biotin, vitamin K, folic acid, inositol, niacin, pantothenic acid, niacin, pyridoxine, choline, and mixtures thereof.

Minerals and trace elements useful as food additives include calcium, phosphorus, sodium, chloride, potassium, magnesium, iron, copper, zinc, selenium, iodine, and mixtures thereof. In certain embodiments, the food compositions can further comprise taurine.

6.5.2.6. Palatability Agents

The food composition of the disclosure may comprise one or more palatability agents. The palatability agents are typically added to a food composition to enhance the overall palatability of the food to overcome any negative effects to flavor or smell.

The palatability agents may be added to enhance mouth feel or attractiveness of the food product, such as dyes or any other colorant that can change the color of the food composition.

A flavoring agent may be a flavoring molecule(s) and/or flavoring precursor(s). Flavoring agents may include carbohydrates, sugars, nucleic acids (e.g., nucleotides and/or nucleosides), free fatty acids, amino acids and/or derivatives, vitamins, minerals, antioxidants, or any combination thereof.

Carbohydrates and sugars may include but are not limited to, glucose, fructose, ribose, sucrose, arabinose, inositol, maltose, molasses, maltodextrin, glycogen, glycol, galactose, lactose, sorbitol, amylose, amylopectin, xylose, or any combination thereof.

Nucleic acids may include but are not limited to, inosine, inosine monophosphate, guanosine, guanosine monophosphate, adenosine, adenosine monophosphate, or any combination thereof. Free fatty acids may include but are not limited to, arachidic acid, behenic acid, caprylic acid, capric acid, cerotic acid, erucic acid, lauric acid, linoleic acid, myristic acid, palmitic acid, palmitoleic acid, stearic acid, lignoceric acid, or any combination thereof.

Amino acids and/or amino acid derivatives may include but are not limited to, cysteine, cystine, cysteine sulfoxide, allicin, selenocystein, methionine, isoleucine, leucine, lysine, phenylalanine, threonine, tryptophan, 5-hydroxy tryptophan, valine, arginine, histidine, alanine, asparagine, aspartate, glutamate, glutamine, glycine, proline, serine, tyrosine, taurine, or any combination thereof. Amino acids may be added to the food product as free amino acids or as amino acid derivatives. For example, any amino acid may be added to the food product as a free amino acid (e.g., pre-digested amino acids without other functional groups of chemical moieties).

Flavoring agents may include, but are not limited to retinol, retinal, beta-carotene, thiamine, riboflavin, niacin, niacinamide, nicotinamide, riboside, pantothenic acid, pyridoxine, pyridoxamine, pyridoxal, biotin, folates, cyanocobalamin, hydroxocobalamin, methylcobalamin, adenosylcobalamin, ascorbic acid, cholecalciferol, ergocalciferol, tocopherols (e.g., alpha-tocopherol), tocotrienols, phylloquinone, menaquinones, potassium, chlorine, sodium, calcium, phosphorus, magnesium, iron, zinc, manganese, copper, iodine, chromium, molybdenum, selenium, cobalt, or any combination thereof.

Antioxidants may include, but are not limited to, beta-carotene, alpha-tocopherol, quercetin, caffeic acid, propyl gallate, epigallocatechin gallate, or any combination thereof.

In some embodiments, zeolite is added to animal food compositions in amounts sufficient to enhance palatability. Preferably in amounts of zeolite that can be added to a food composition range from about 0.01% to about 4% by weight of the food composition.

6.5.3. Pet Food and Feed Compositions

Various pet foods (companion animals) and animal feed (livestock, zoo animal) compositions are also provided. A pet food or animal feed composition can be made by combining a recombinant animal protein provided herein with a variety of other ingredients (as provide in Section 6.5.2) and/or additives or preservatives to generate a pet food or feed product. The one or more ingredients may be a wet ingredient, a dry ingredient, or other ingredients as provided herein, or any combination thereof. The pet food can be in various formats such as a kibble, a freeze-dried food product, a dehydrated food product, a baked food product, or raw formats.

6.5.3.1. Food or Feed Formulations

The food or feed product can be made in various formulations. The amount of the other ingredients can be mixed with the recombinant animal protein to make the food or feed formulation will depend on the dietary requirements of a companion animal, livestock, zoo animal, which can depend on the species, age, size, weight, growth stage, health condition, and/or organ function (e.g., liver, heart, join, hip, or brain) of the animal.

In some embodiments, the pet food of feed comprising a recombinant animal protein is formulated to be nutritionally balanced. As used herein, the term “nutritionally balanced,” with reference to the pet food or feed composition, means that the composition has known required nutrients based on recommendations of recognized authorities in the field of pet nutrition.

For example, the recommended nutrients and their amounts have been established for various animals. See, National Research Council (NRC) provides recommended amounts of such nutrients for farm animals; nutrient Requirements of Swine (11th Rev. Ed., National Academy Press, Wash. D.C., 2012); Nutrient Requirements of Poultry (9th Rev. Ed., National Academy Press, Wash. D.C., 1994); Nutrient Requirements of Horses (6th Rev. Ed., National Academy Press, Wash. D.C., 2007), each of which are incorporated in their entirety.

The American Feed Control Officials (AAFCO) provides recommended amounts of such nutrients for dogs and cats. See American Feed Control Officials, Inc. (Official publication, 2018). In some embodiments, the food product comprises the AAFCO nutrient profile established for a dog. In some embodiments, the food product comprises the AAFCO nutrient profile established for a cat.

In some embodiments, the feed comprises at least the minimum or the maximum nutrient concentrations as established by NRC for various farm animals, pig, sheep, chicken, horse, goat, and the like.

Preferably, the food composition will include, by mass, 5-50% protein, 0.01-1.5% sodium, 0.01-1.5% potassium, 0-50% fat, 0-75% carbohydrate, 0-40% dietary fiber, and 0-15% of other nutrients.

The food product comprising a recombinant protein composition can be formulated into a breed-specific food formulation. In some embodiments, the proteins for breed-specific food formulations can be based on growth rate. See for example U.S. Pat. No. 5,851,573, which is hereby incorporated by reference in its entirety. In some embodiments, the proteins for breed-specific food formulations can be based on phenotypic characteristics of the animal. See for example U.S. Pat. No. 6,669,975, which is hereby incorporated by reference in its entirety. In some embodiments, the proteins for breed-specific food formulations can be based on genomic methods. See for example US Publication No. 20060045909, which is hereby incorporated by reference in its entirety.

In some embodiments, the food or feed product can be formulated into a product that improves health or wellness. In some embodiments, the food or feed further comprises a compound that improves joint function, skin health, coat or hair, brain development, or improves stool quality and/or stool frequency.

6.5.3.2. Form and Shape

The pet food or feed product (dry or wet) can be in any form useful for feeding the food composition to an animal. The food product may be a shaped and/or molded or non-shaped product. For example, the food product may comprise shaped treats, kibble, edible granules, or made into a toy-shaped food product.

The pet food or feed product may be formulated for mouthfeel. Mouthfeel of the pet food product may be formulated according to its structure, dryness, density, adhesiveness, bounce, chewiness, coarseness, cohesiveness, fracturability, graininess, gumminess, hardness, heaviness, moisture adsorption, moisture release, mouthcoating, roughness, slipperiness, smoothness, springiness, uniformity, and viscosity.

The pet food or feed product may be formulated to have a porous, fibrous, or amorphous structure. In an example, the pet food product has a fibrous structure. The pet food product may be formulated for fracturability such that the product crumbles, cracks, or shatters. Fracturability may encompass crumbliness, crispness, crunchiness, and brittleness.

6.5.3.3. Dry Pet Food and Feed

In some embodiments, the food product is a dry pet food or feed product for a companion animal, or dry feed for livestock, zoo animal or a pet.

The dry pet food or feed product can be made completely of the recombinant animal protein. In some other embodiments, the dry pet food can comprise about 1%, 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, 10%, 15%, 20%, 25%, 30%, 35%, 40%, 45%, 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, or 90% of the recombinant animal protein.

A dry pet food or feed product can be prepared by adding one or more dry ingredients. Other ingredients that can be added to a dry food product include but are not limited to the ingredients provided in Section 6.5.2.

The dry pet food or feed product can be freeze-dried, dehydrated, or air-dried. In some embodiments, the recombinant animal protein can be a coating on another dry food product. In some embodiments, the dry food product is a kibble.

The dry pet food or feed can have the nutrient profile required for a dog or cat as provided by the AAFCO guidelines. In some embodiments, the dry feed has the nutrient profile as established by NRC for various farm animals.

Kibbles are generally formed using an extrusion process in which the mixture of dry and wet ingredients is mechanically worked at high temperature and pressure and pushed through small openings and cut off into kibble by a rotating knife. Kibble also can be made using a baking process when the mix is placed into a mold before dry-heat treatment.

In some embodiments, the recombinant animal protein composition is coated onto the dry kibble, incorporated into the kibble, or both. Other processes such as spraying, soaking, or brushing may be used to either coat the composition on the exterior or inject the recombinant animal protein composition into an existing dry kibble.

6.5.3.4. Wet Pet Food and Feed

The disclosure also provides wet pet food products for a companion animal, or wet feed for livestock or a zoo animal. A wet pet food or feed can be prepared by adding one or more wet ingredients such as water containing host cells comprising recombinant animal protein, water, oils, fats, or vegetables or a combination thereof. Other non-limiting ingredients that can be added to a dry food product are provided in Section 6.5.2. In some embodiments, the wet food product is raw.

The wet pet food or feed can be made completely of the recombinant animal protein. In some other embodiments, the wet pet food or feed can comprise about 1%, 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, 10%, 15%, 20%, 25%, 30%, 35%, 40%, 45%, 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, or 90% of the recombinant animal protein.

The wet pet food or feed can have the nutrient profile required for a dog or cat as provided by the AAFCO guidelines. In some embodiments, the wet feed has the nutrient profile as established by NRC for various farm animals.

The wet kibble can be a dried kibble that is coated with one or more wet topical coatings supplied as intermediate food product of the disclosure. In some embodiments, wet kibble can be made by mixing the kibble into a gravy-like liquid supplied as an intermediate food product of the disclosure.

6.5.3.5. Pet Treats

The disclosure also provides treats for a companion animal, livestock, or a zoo animal. The treat can be a dry treat, an edible toy, or a chewable toy. The treat can be made completely of the recombinant animal protein. In some other embodiments, the treat can comprise about 1%, 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, 10%, 15%, 20%, 25%, 30%, 35%, 40%, 45%, 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, or 90% of the recombinant animal protein.

Treats of the present invention can be prepared by an extrusion or baking process similar to those used for dry food. Treats of the disclosure can be prepared by a molding process. Treats can also be in the form of a chew toy. Chewable toys can include but are not limited to, artificial bones and food compositions shaped to look like natural foods that are appealing to the animal.

Often, a pet treat will have nutritional value. Nutritional treats may contain one or more nutrients required for a primary food product. Non-nutritional treats can have minimal nutrition of a primary food product. Treat may also be mixed with other ingredients. Other non-limiting ingredients that can be added to a pet treat include provided in Section 6.5.2.

In some embodiments, the treat further comprises a compound that improves health or wellness. In some embodiments, the treat furthers comprise a compound that improves joint function, skin health, coat or hair, brain development, or improves stool quality and/or stool frequency.

In some embodiments, the recombinant animal protein composition is coated onto the treat, incorporated into the treat, or both. Other processes such as spraying, soaking, or brushing may be used to either coat the recombinant animal protein as an intermediate food product composition on the exterior of the treat or inject it into an existing treat form.

6.5.3.6. Packaging

The food compositions can be packaged in cans, trays, tubs, pouches, bags, or any other suitable container.

6.6. Supplements

The disclosure provides supplements for a human or animal. A dietary supplement is a product intended to supplement the diet. The recombinant animal protein can be harvested and provided to the supplement composition as a whole-cell food composition, a protein concentrate food composition, or as a protein isolate food composition.

In some embodiment the supplement is made solely from at least one animal protein provided by the disclosure. In other embodiments, the animal protein is combined with other ingredients or nutrients. Other ingredients include but are not limited to those in Section 6.5.2.

In some embodiments, a supplement can be taken by mouth. Where a supplement is formulated to be taken by mouth, it can be in the form of a pill, a capsule, a tablet, a liquid, soup, broth, or a dissolvable powder. In some embodiment, the supplement can a dry protein mixture of one or more recombinant animal proteins.

In other embodiments, a supplement can be incorporated into a commercially available food product. In some embodiments, the recombinant animal proteins is incorporated into a commercially available food product at a percentage (based on dry mass) of 0.1-95%, typically between 10% and 90%, more typically between 5% and 50%, including ranges of 5%-10%, 10-20%, 20-30%, 30-40%, 40-50%, but also including 60-70%, 70-80% and 80%-90% and combinations of these ranges (e.g., 30%-70%).

In some embodiments, the recombinant animal protein can be incorporated into commercially available food product to increase the percentage of an essential amino acid in the product. The percentage of one or more essential amino acids can be increased in a commercially available food product by about 0.1%, 0.2%, 0.3%, 0.4%, 0.5%, 0.6%. 0.7%, 0.8%, 0.9%, 1%, 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, or 90% (based on dry mass).

6.7. Pharmaceutical Compositions

The disclosure also provides various pharmaceutical compositions comprising a recombinant animal protein of the disclosure that improves the health or wellness of a human or an animal.

These compositions can comprise, in addition to the recombinant animal protein, a pharmaceutically acceptable excipient, carrier, buffer, stabilizer or other materials well known to those skilled in the art. Such materials should be non-toxic and should not interfere with the efficacy of the active ingredient.

The precise nature of the carrier or other material can depend on the route of administration, e.g., oral, intravenous, cutaneous or subcutaneous, nasal, intramuscular, or intraperitoneal routes

6.7.1. Improves Health or Wellness

A pharmaceutical composition can be made by combining a recombinant animal protein provided herein with a compound known or capable of improving the health or the wellness of an animal.

In some embodiments, the pharmaceutical composition comprises a recombinant animal protein of the disclosure and a compound that improves hip function.

In some embodiments, the pharmaceutical composition comprises a recombinant animal protein of the disclosure and a compound that improves joint function. In some embodiments, the pharmaceutical composition comprises a recombinant animal protein of the disclosure and a compound that improves skin health. In some embodiments, the pharmaceutical composition a recombinant animal protein of the disclosure and a compound that improves coat or hair. In some embodiments, the pharmaceutical composition comprises a recombinant animal protein of the disclosure and a compound that improves brain development. In some embodiments, the pharmaceutical composition comprises a recombinant animal protein of the disclosure and a compound that improves stool quality and/or stool frequency.

Wellness of an animal herein encompasses all aspects of the physical, mental, and social well-being of the animal, and is not restricted to the absence of infirmity. Wellness attributes include without limitation states of disease or physiological disorder, states of parasitic infestation, hair and skin condition, sensory acuteness, dispositional and behavioral attributes, and cognitive function. Conditions adverse to wellness encompass not only existing diseases and physiological including, mental, behavioral, and dispositional disorders, but predisposition or vulnerability to such diseases or disorders. Asymptomatic are likewise encompassed.

6.7.2. Formulations

Pharmaceutical compositions for oral administration can be in tablet, capsule, powder or liquid form. A tablet can include a solid carrier such as gelatin or an adjuvant. In some embodiments, the capsule can be made from a vegetarian material such as agar, vegetable cellulose, and the like. Liquid pharmaceutical compositions generally include a liquid carrier such as water, petroleum, animal or vegetable oils, mineral oil or synthetic oil. Physiological saline solution, dextrose or other saccharide solution or glycols such as ethylene glycol, propylene glycol or polyethylene glycol can be included.

For intravenous, cutaneous or subcutaneous injection or injection at the site of affliction, the active ingredient will be in the form of a parenterally acceptable aqueous solution which is pyrogen-free and has suitable pH, isotonicity and stability. Those of relevant skill in the art are well able to prepare suitable solutions using, for example, isotonic vehicles such as Sodium Chloride Injection, Ringer's Injection, Lactated Ringer's Injection. Preservatives, stabilizers, buffers, antioxidants and/or other additives can be included, as required.

In some embodiments, the pharmaceutical composition can be in the form of nutritional feed, a food product, or a treat.

6.7.3. Methods of Treating

The disclosure also provides methods of treatment for an animal diagnosed or suffering from a disease or disorder.

The method can comprise administering a therapeutic-effective amount of the pharmaceutical composition provided herein alone or in combination with another agent or treatment to promote health or wellness.

In some embodiments, the method includes administering a therapeutically effective amount of the pharmaceutical composition to an animal diagnosed or suffering from a disease or disorder. In yet some other embodiments, the method includes administering a prophylactically effective amount of the pharmaceutical composition to an animal genetically predisposed to a disease or a disorder.

A genetically predisposed animal can be based on the breed, age, size, or any other physical characteristic.

6.7.4. Administration

For treatment purposes, administration of the pharmaceutical composition is preferably administered to an animal in a “therapeutically effective amount.” In some embodiments, the pharmaceutical composition is preferably administered to an animal in a “prophylactically effective amount” to the animal or individual.

The actual amount administered, and rate and time-course of administration will depend on the nature and severity of disease or disorder being treated.

Prescription of treatment, e.g., decisions on dosage, etc., is within the responsibility of general practitioners and other medical doctors, and typically takes account of the disorder to be treated, the condition of the individual patient, the site of delivery, the method of administration and other factors known to practitioners. Examples of the techniques and protocols mentioned above can be found in Remington's Pharmaceutical Sciences, 16th edition, Osol, A. (ed.), 1980.

6.7.5. Combination Therapy

This disclosure also provides combination therapies where the pharmaceutical composition is administered in combination with another therapeutic agent or treatment.

In some embodiments, the pharmaceutical composition can be administered either simultaneously or sequentially, dependent upon the condition to be treated.

Non-limiting examples of a therapeutic treatment include physical therapy, surgery, radiation, and dietary restrictions for diseases such as diabetes.

6.8. Additional Aspects & Embodiments

In a first additional aspect, the disclosure provides various food compositions comprising at least one recombinant animal protein.

Embodiment 1. A food composition, wherein said food composition is formulated for a companion animal, and wherein the food composition comprises at least one recombinant animal protein.

Embodiment 2. The food composition of embodiment 1, wherein the food composition is substantially free of antibiotics, animal growth hormones, and processed animal meat.

Embodiment 3. The food composition of any of the above embodiments, wherein the at least one recombinant animal protein is a recombinant animal muscle protein.

Embodiment 4. The food composition of embodiment 3, wherein the at least one recombinant animal muscle protein is selected from the animal muscle proteins in Table 1.

Embodiment 5. The food composition of embodiment 4, wherein the food composition comprises at least two recombinant animal muscle proteins.

Embodiment 6. The food composition of any of the above embodiments, wherein at least one recombinant animal muscle protein comprises a modified amino acid sequence, wherein said modification is relative to the naturally occurring sequence of the animal muscle protein.

Embodiment 7. The food composition of embodiment 6, wherein said modified recombinant animal muscle protein comprises an amino acid sequence at least 80% identical to a sequence in Table 1.

Embodiment 8. The food composition of embodiment 6, wherein said modified recombinant animal muscle protein is a truncated form of a sequence in Table 1.

Embodiment 9. The food composition of embodiment 6, wherein said modified recombinant animal muscle protein comprises a heterologous signal peptide.

Embodiment 10. The food composition of any of the above claims, wherein the food composition consists of 5% to 95% recombinant animal protein, on a mass percentage basis.

Embodiment 11. The food composition of embodiment 10, wherein the food composition consists of 5% to 40% recombinant animal protein, on a mass percentage basis.

Embodiment 12. The food composition of embodiment any of the above claims, wherein the food composition includes 5-50% protein, 0.01-1.5% sodium, 0.01-1.5% potassium, 0-50% fat, 0-75% carbohydrate, 0-40% dietary fiber, and 0-15% of other nutrients.

Embodiment 13. The food composition of embodiment 10, wherein the food composition consists of 40% to 95% recombinant animal protein.

Embodiment 14. The food composition of embodiment 10, wherein the food composition consists of 1% to 30% recombinant animal protein.

Embodiment 15. The food composition of any of the above embodiments, wherein the food composition is formulated for a dog or a cat.

Embodiment 16. The food composition of any of the above embodiments, wherein the food composition is customized for a particular companion animal or a selected cohort of companion animals with particular dietary needs.

In a second additional aspect, the disclosure provides methods for preparing the food compositions described herein.

Embodiment 17. A method for preparing any of the food compositions described above, wherein the method comprises recombinantly expressing at least one recombinant animal protein in a eukaryotic host organism.

Embodiment 18. The method of embodiment 17, wherein the eukaryotic host organism is a yeast cell.

Embodiment 19. The method of embodiment 17 or 18, wherein the recombinantly expressed animal protein is secreted by the eukaryotic host organism.

Embodiment 20. The method of any one of embodiments 17-19, wherein the recombinantly expressed animal protein is isolated from the host organism and the growth medium before mixing with other components in the food composition.

Embodiment 21. The method of embodiment 20, further comprising mixing the at least one recombinantly expressed animal protein with one or more food components selected from the group consisting of sodium, potassium, fat, carbohydrate, and dietary fiber, and then forming the mixture into a food composition suitable for consumption by an animal.

Embodiment 22. The method of embodiment 21, wherein at least two animal proteins are recombinantly expressed in a eukaryotic host and isolated prior to mixing with the one or more food components.

Embodiment 23. The method of embodiment 17 or 18 wherein the recombinantly expressed protein is not isolated from the host organism prior to mixing with other components in the food composition.

In a third additional aspect, the disclosure provides additional methods for preparing the food compositions described herein.

Embodiment 24. A method for preparing any of the food compositions described above, wherein the method comprises mixing at least one recombinantly expressed animal muscle protein with one or more compositions selected from the group consisting of sodium, potassium, fat, carbohydrate, and dietary fiber, and then forming the mixture into a food composition suitable for consumption by an animal.

In a fourth additional aspect, the disclosure provides additional formulations of food compositions comprising at least one recombinant animal protein.

Embodiment 25. A food composition, wherein said food composition is formulated for a human, and wherein the food composition comprises at least one recombinant animal muscle protein.

6.9. Examples

Below are examples of specific embodiments for carrying out the present invention. The examples are offered for illustrative purposes only and are not intended to limit the scope of the present invention in any way. Efforts have been made to ensure accuracy with respect to numbers used (e.g., amounts, temperatures, etc.), but some experimental error and deviation should, of course, be allowed for.

The practice of the present invention will employ, unless otherwise indicated, conventional methods of protein chemistry, biochemistry, recombinant DNA techniques and pharmacology used in the art. Also referred to below are the following references: (1) M. R. Green and J. Sambrook, Molecular Cloning: A Laboratory Manual, 4th Edition, Cold Spring Harbor, N.Y.: Cold Spring Harbor Laboratory Press, 2012, pp. 1009-1011; (2) G. C. U. F. T. Tool, GenScript, [Online]. Available: https://www.genscript.com/tools/codon-frequency-table. [Accessed 18 12 2018]; (3) S. Wu and L. J. Geoffrey, “High efficiency transformation by electroporation of Pichia pastoris pretreated with lithium acetate and dithiothreitol,” Drug Discovery and genomic technologies, vol. 36, no. 1, pp. 152-154, 2004; (4) S. Kawai, W. Hashimoto and K. Murata, “Transformation of Saccharomyces cerevisiae and other fungi,” Bioengineered Bugs, vol. 1, no. 6, pp. 395-403, 2010; (5) P. Manivasakam and R. H. Schiestl, “High efficiency transformation of Saccharomyces cerevisiae by electroporation,” Nucleic Acids Research, vol. 21, no. 18, pp. 4414-4415, 1993.

6.9.1. Example 1: Expression of Recombinant Actin Protein in a Eukaryotic Host Cell

Actin is the major component of the cytoskeleton. It exists in two different forms, a monomeric form (G-actin) and a filamentous form (F-actin). G-actin polymerizes to form F-actin, and it is primarily these filaments that participate in processes such as cell motility, transport, and cytokinesis [20]. The actin-binding domain is highly conserved amongst species. Actin-binding proteins share a common binding area on the actin surface, consistent of the cleft between actin subdomains 1 and 3 [21]. There is also a nucleotide-binding site, which is a cleft between subdomains 2 and 4. The binding of adenosine 5′-triphosphate or ATP and subsequent hydrolysis into adenosine 5′-diphosphate or ADP is known to be a critical element in controlling the association of actin with itself and with other proteins. When ATP is bound to actin it polymerizes faster and dissociates slower than ADP-actin [22].

Single and double mutants of the ATP-binding site of actin will ablate its toxicity in eukaryotic expression hosts and thus increase expression levels. The residues targeted by mutagenesis are P-72, E-74, 1-77, and T-79 (numbering for pig (UniProtKB/Swiss-Prot: P68137), chicken (UniProtKB/Swiss-Prot:P68139), and cow (UniProtKB/Swiss-Prot:P68138)). Recombinant actin protein mutated at these sites will be over-expressed in a eukaryotic host organism, isolated, and incorporated into a companion animal food product.

In one embodiment, then, the invention provides a food composition comprising a recombinant actin protein, wherein said recombinant actin protein comprises one or more mutations from the group consisting of P-72, E-74, 1-77 and T-79. In certain related embodiments, the recombinant actin protein is a fragment of actin protein comprising the aforementioned residues.

6.9.2. Example 2: Identification of Recombinant Actin Sequences for Over-Expression in Eukaryotes

Actin is highly conserved between widely divergent eukaryotic species. For instance, there is 87% sequence identity (325 of 374 amino acids) between yeast and human actin. Comparing chicken, cow, pig, human, and Saccharomyces cerevisiae, there are 319 conserved residues. A library of point mutations is made at each of these conserved positions and those mutations that are permissive of high levels of expression of mutant actin are identified.

6.9.3. Example 3: Engineered Animal Proteins for Over-Expression in Eukaryotic Cells

Error-prone PCR with/without shuffling will be used across the DNA coding sequence (cDNA) to create mutated DNAs encoding animal protein sequences. Eukaryotic hosts recombinantly expressing the mutant sequences will be screened for high growth and high expression of the target protein.

The genes and the proteins encoded by the genes may also be truncated in order to yield a high expression and fast cell growth. Modifications of the gene sequence (e.g., the addition or removal of certain amino acids) will, in some cases, increase cell viability and increase the rate of cell division. Proteins that are too large to overexpress efficiently will be truncated in order to increase the expression level.

6.9.4. Example 4: Insertion of a Chicken Coronin Gene into an Saccharomyces cerevisiae Strain and Extracellular Expression of the Corresponding Protein

The expression vector pD1214-FAKS (ATUM) contains the 2-micron origin of replication, which encodes proteins that allow yeast cells to maintain 20-50 copies of recombinant plasmid per cell. Because 2-micron plasmids are maintained at such high copy numbers, they provide a convenient way to monitor the effects of overproduction of a particular gene product. The plasmid also contains a bacterial origin of replication (Ori_pUC) which allows production of greater than 500 copies of plasmid per cell in Escherichia coli. It is replicated in Escherichia coli TOP10 cells grown in Low Salt Luria-Bertani medium (5 g/L NaCl) including 100 μg/mL carbenicillin as selective pressure at 37° C. The vector also contains the alpha factor, which is a secretion signal derived from the yeast mating pheromone alpha-factor in Saccharomyces cerevisiae and facilitates secretion of heterologous proteins in yeast. The plasmid is purified from E. coli by methods well known in the art, using for instance a commercially available plasmid prep kit, such as the QIAGEN Plasmid Mini Kit. The vector is linearized using a SapI restriction enzyme followed by enzymatic dephosphorylation using established molecular cloning methods. The gene encoding the protein product of interest, chicken coronin, can also be ordered in the selected vector from contract cloning vendors such as ATUM (Newark, Calif.). This plasmid contains features such as the strong constitutive promoter TEF1, encoding translation-elongation factor 1 alpha and the gene coding for ampicillin resistance (beta lactamase). The vector also contains an auxotrophic marker URA3, which encodes orotidine-5′ phosphate decarboxylase, an enzyme that is required for the biosynthesis of uracil.

Linearized plasmid is separated using agarose gel electrophoresis. An agarose gel section containing linearized plasmid is collected and the linearized plasmid is purified from the agarose using a commercially available DNA purification kit, e.g. the QIAquick Gel Extraction Kit (Qiagen).

The gene sequence for chicken coronin can be obtained from UniProt.org under accession number F1NXA5. The double-stranded DNA is constructed through chemical gene synthesis from either ATUM (Newark, Calif.), Genscript (Piscataway, N.J.), or IDT (Coralville, Iowa). It is supplied in a vector of choice. The DNA sequence can also be obtained via amplification of cDNA generated directly from a biological sample, such as a tissue or a blood sample. The gene sequence is modified to aid in cloning, gene expression, or enhance production. It is “codon optimized”, i.e., triplet DNA sequences that are not commonly used in the expression host are changed to those that are commonly used. The specific species in this case is Saccharomyces cerevisiae and the codon usage table is obtained from GenScript.

The codon optimized coronin gene (CORO6), containing exons, but no introns, is ligated to the linearized and purified vector via enzymatic ligation to generate a vector capable of being inserted into a host organism. Electroporation and other methods of transformation are well known in the art. The vector containing the ORF is transformed into the host strain (S. cerevisiae, in this example) via electroporation using of 1.5 kV, 25 μF, and 200Ω. Chemical transformation or another method can also be used. Transformed cells are plated onto minimal media lacking uracil and incubate at 30° C. until heterotrophic colonies arise in 2-3 days. Colonies are picked and transferred into cultures of minimal media or YPD and grown for 24-90 hours at 28-30° C. The successful clone is confirmed by sequencing for insert identity and copy number using established methods such as PCR, q-PCR, or Southern Blot.

The supernatant is analyzed for secreted protein expression by SDS-PAGE. Isolated clones expressing the secreted protein will be cultured, and the recombinantly expressed protein is isolated from the engineered yeast cells or, if secreted, is isolated from the medium. The secreted, recombinantly expressed protein is then formulated into a food composition for animals, preferably companion animals. In one embodiment, then, the disclosure provides a food composition comprising a recombinantly expressed chicken coronin protein. In certain embodiments, the recombinantly expressed chicken coronin protein is harvested from yeast cultures, wherein the yeast has been engineered to express the protein.

6.9.5. Example 5: Insertion of a Pig Myozenin Gene into a Komagataella phaffii Strain and Intracellular Expression of the Corresponding Protein

The expression vector pD902 (ATUM, Newark, Calif.) contains a bacterial origin of replication (OripUC) which allows production of greater than 500 copies of plasmid per cell in Escherichia coli. It is replicated in Escherichia coli TOP10 cells grown in Low Salt Luria-Bertani medium (5 g/L NaCl) including 25 μg/mL zeocin as selective pressure at 37° C. The plasmid is purified by a method well known in the art, using for instance a commercially available plasmid prep kit, such as the QIAGEN Plasmid Mini Kit. The vector is linearized using a SapI restriction enzyme and performing dephosphorylation using established molecular cloning methods [1]. The gene can also be ordered in the selected vector. This plasmid contains features such as the AOX1 promoter used for recombinant gene expression and the resistance marker for zeocin. Linearized plasmid is separated using agarose gel electrophoresis. An agarose gel section containing linearized plasmid is collected and the linearized plasmid is purified from the agarose using a commercially available DNA purification kit, e.g. the QIAquick Gel Extraction Kit (Qiagen).

The gene sequence for pig myozenin can be obtained from UniProt.org under accession number Q4PS85. The double-stranded DNA is constructed through chemical gene synthesis from either ATUM (Newark, Calif.), Genscript (Piscataway, N.J.), or IDT (Coralville, Iowa). It is supplied in a vector of choice. The DNA sequence can also be obtained via amplification of cDNA generated directly from a biological sample, such as a tissue or a blood sample. The gene sequence is modified to aid in cloning, gene expression, or enhance production. It is “codon optimized”, i.e. triplet DNA sequences that are not commonly used in the expression host are changed to those that are commonly used. The specific species in this case is Komagataella phaffii (previously Pichia pastoris) and the codon usage table is obtained from GenScript [2]. The strain PPS-9016 is protease-deficient (ATUM, Newark, Calif.). Other variants of Komagataella phaffii can also be used.

The codon optimized myozenin gene (MYOZ1), containing exons, but no introns, is ligated to the linearized and purified vector via enzymatic ligation to generate a vector capable of being inserted into a host organism. The method used is known in the art and the protocol can be obtained from a molecular cloning manual [1]. The vector containing the gene, also called ORF open reading frame) is linearized using the PmeI restriction enzyme. Twenty micrograms of DNA are digested using the corresponding buffer of the restriction enzyme (from e.g. NEB) in a volume of 200 μL. Five μL of digested DNA is run on a 1% agarose gel and compared with an undigested control. The digested product is ethanol precipitated using 1/10 volume of 3M sodium acetate and 2.5 volumes of 100% ethanol. It is centrifuged to pellet the DNA and pellet is washed with 70% ethanol, air dried, and suspended in 20 μL of deionized sterile water or 10 mM Tris-Cl, pH 8.0. The linearized vector containing the ORF is transformed into the host strain.

Transformation is performed via electroporation using instrument settings of 1.5 kV, 25 μF, and 186-200Ω. Electrocompetent cells are obtained via methods known in the art [3]. Chemical transformation or another method can also be used. The vector containing the ORF is integrated into the chromosome of the host organism. The vector does not contain a yeast origin of replication and selected transformants, grown at 30° C. on YPD agar plates containing 100-1000 μg/mL zeocin and 1 M sorbitol, will contain the zeocin resistance gene integrated into the genome. Multiple insertions of the gene may be used. The successful clone is confirmed by sequencing for insert identity and copy number using established methods such as PCR, q-PCR, or Southern Blot [1].

Colonies are picked into BMGY broth with 250 μg/ml zeocin and are grown at 30° C. shaking at 250 rpm. After 2 days of incubation, 300 μL of BMMY broth is added to each well, and incubation is continued for an additional 2-4 days. The cells are pelleted by centrifugation and the cell pellets are lysed by methods known in the art, e.g. by sonication [1] and analyzed for protein expression by SDS-PAGE.

6.9.6. Example 6: Insertion of a Pig Myozenin Gene into a Komagataella phaffii Strain and Extracellular Expression of the Corresponding Protein

The expression vector pD912 (ATUM, Newark, Calif.) contains a bacterial origin of replication (Ori_pUC) which allows production of greater than 500 copies of plasmid per cell in Escherichia coli. It is replicated in Escherichia coli TOP10 cells grow in in Low Salt Luria-Bertani medium (5 g/L NaCl) including 25 μg/mL zeocin as selective pressure at 37° C. The vector also contains the alpha factor, which is a secretion signal derived from the yeast mating pheromone alpha-factor in Saccharomyces cerevisiae and facilitates secretion of heterologous proteins in yeast. The plasmid is purified by a well-known method, using for instance a commercially available plasmid prep kit, such as the QIAGEN Plasmid Mini Kit. The vector is linearized using a SapI restriction enzyme and performing dephosphorylation using established molecular cloning methods [1]. The gene can also be ordered in the selected vector. This plasmid contains features such as the AOX1 promoter used for recombinant gene expression and the resistance marker for zeocin. Linearized plasmid is separated using agarose gel electrophoresis. An agarose gel section containing linearized plasmid is collected and the linearized plasmid is purified from the agarose using a commercially available DNA purification kit, e.g. the QIAquick Gel Extraction Kit (Qiagen).

The gene sequence for pig myozenin can be obtained from UniProt.org under accession number Q4PS85. The double-stranded DNA is constructed through chemical gene synthesis from either ATUM (Newark, Calif.), Genscript (Piscataway, N.J.), or IDT (Coralville, Iowa). It is supplied in a vector of choice. The DNA sequence can also be obtained via amplification of cDNA generated directly from a biological sample, such as a tissue or a blood sample. The gene sequence is modified to aid in cloning, gene expression, or enhance production. It is “codon optimized”, i.e. triplet DNA sequences that are not commonly used in the expression host are changed to those that are commonly used. The specific species in this case is Komagataella phaffii (previously Pichia pastoris) and the codon usage table is obtained from GenScript [2]. The strain PPS-9016 is protease-deficient (ATUM, Newark, Calif.). Other variants of Komagataella phaffii can also be used.

The codon optimized myozenin gene (MYOZ1), containing exons, but no introns, is ligated to the linearized and purified vector via enzymatic ligation to generate a vector capable of being inserted into a host organism. The method used is known in the art and the protocol can be obtained from a molecular cloning manual [1]. The vector containing the gene, also called ORF open reading frame) is linearized using the PmeI restriction enzyme. Twenty micrograms of DNA are digested using the corresponding buffer of the restriction enzyme (from e.g. NEB) in a volume of 200 μL. Five μL of digested DNA is run on a 1% agarose gel and compared with an undigested control. The digested product is ethanol precipitated using 1/10 volume of 3M sodium acetate and 2.5 volumes of 100% ethanol. It is centrifuged to pellet the DNA and pellet is washed with 70% ethanol, air dried, and suspended in 20 μL of deionized sterile water or 10 mM Tris-Cl, pH 8.0. The linearized vector containing the ORF is transformed into the host strain via electroporation using instrument settings of 1.5 kV, 25 μF, and 186-200Ω. Electrocompetent cells are obtained via methods known in the art [3]. Chemical transformation or another method can also be used. The vector containing the ORF is integrated into the chromosome of the host organism. The vector does not contain a yeast origin of replication and selected transformants, grown at 30° C. on YPD agar plates containing 100-1000 μg/mL zeocin and 1 M sorbitol, will contain the zeocin resistance gene integrated into the genome. Multiple insertions of the gene may be used. The successful clone is confirmed by sequencing for insert identity and copy number using established methods such as PCR, q-PCR, or Southern Blot [1].

Colonies are picked into BMGY broth with 250 μg/ml zeocin and are grown at 30° C. shaking at 250 rpm. After 2 days of incubation, 300 μL of BMMY broth is added to each well, and incubation is continued for an additional 2-4 days. The supernatant is analyzed for secreted protein expression by SDS-PAGE.

6.9.7. Example 7: Insertion of a Chicken Coronin Gene into an Saccharomyces cerevisiae Strain and Intracellular Expression of the Corresponding Protein

The expression vector pD91248 (ATUM, Newark, Calif.) contains a bacterial origin of replication (OripUC) which allows production of greater than 500 copies of plasmid per cell in Escherichia coli. It is replicated in Escherichia coli TOP10 cells grown in Low Salt Luria-Bertani medium (5 g/L NaCl) including 100 μg/mL carbenicillin as selective pressure at 37° C. The plasmid is purified by a method well known in the art, using for instance a commercially available plasmid prep kit, such as the QIAGEN Plasmid Mini Kit. The vector is linearized using a SapI restriction enzyme and performing dephosphorylation using established molecular cloning methods [1]. The gene can also be ordered in the selected vector. This plasmid contains features such as the bidirectional galactose inducible promoter cassette pGAL1/pGAL10 and the gene coding for ampicillin resistance (beta lactamase). The vector also contains an auxotrophic marker URA3, which encodes orotidine-5′ phosphate decarboxylase, an enzyme that is required for the biosynthesis of uracil.

Linearized plasmid is separated using agarose gel electrophoresis. An agarose gel section containing linearized plasmid is collected and the linearized plasmid is purified from the agarose using a commercially available DNA purification kit, e.g. the QIAquick Gel Extraction Kit (Qiagen).

The gene sequence for chicken coronin can be obtained from UniProt.org under accession number F1NXA5. The double-stranded DNA is constructed through chemical gene synthesis from either ATUM (Newark, Calif.), Genscript (Piscataway, N.J.), or IDT (Coralville, Iowa). It is supplied in a vector of choice. The DNA sequence can also be obtained via amplification of cDNA generated directly from a biological sample, such as a tissue or a blood sample. The gene sequence is modified to aid in cloning, gene expression, or enhance production. It is “codon optimized”, i.e. triplet DNA sequences that are not commonly used in the expression host are changed to those that are commonly used. The specific species in this case is Saccharomyces cerevisiae and the codon usage table is obtained from GenScript [2].

The codon optimized coronin gene (CORO6), containing exons, but no introns, is ligated to the linearized and purified vector via enzymatic ligation to generate a vector capable of being inserted into a host organism. The method used is known in the art and the protocol can be obtained from a molecular cloning manual [1]. The vector containing the gene, also called ORF (open reading frame) is linearized using the NcoI restriction enzyme. Twenty micrograms of DNA are digested using the corresponding buffer of the restriction enzyme (from e.g. NEB) in a volume of 200 μL. Five μL of digested DNA is run on a 1% agarose gel and compared with an undigested control. The digested product is ethanol precipitated using 1/10 volume of 3M sodium acetate and 2.5 volumes of 100% ethanol. It is centrifuged to pellet the DNA and pellet is washed with 70% ethanol, air dried, and suspended in 20 μL of deionized sterile water or 10 mM Tris-Cl, pH 8.0. The linearized vector containing the ORF is transformed into the host strain.

Transformation is performed via electroporation using instrument settings of 1.5 kV, 25 μF, and 186-200Ω. Electrocompetent cells are obtained via methods known in the art [3]. Chemical transformation or another method can also be used. The vector containing the ORF is integrated into the chromosome of the host organism. The vector does not contain a yeast origin of replication and selected transformants, grown at 30° C. on CM agar minus uracil will contain the URA3 gene integrated into the genome. Incubate at 30° C. until colonies arise in 2-3 days. Multiple insertions of the gene may be used. The successful clone is confirmed by sequencing for insert identity and copy number using established methods such as PCR, q-PCR, or Southern Blot [1].

Colonies are picked into YPD broth and are grown at 28-30° C. shaking at 250 rpm for 24-90 hours. The cells are pelleted by centrifugation and the cell pellets are lysed by methods known in the art, e.g. by sonication [1] and analyzed for protein expression by SDS-PAGE.

6.9.8. Example 8: Production of Recombinant Cofilin-2 Protein from Chicken in an Saccharomyces cerevisiae Host Cell

This study was conducted to determine if an Saccharomyces cerevisiae host cell could produce a cofilin-2 protein from chicken.

Cofilin-2 reversibly controls actin polymerization and depolymerization in a pH-sensitive manner. The particular protein used here is muscle-specific.

Methods

Identification of the Cofilin-2 Gene Sequences from a Chicken Genome

The sequence for the cofilin-2 gene in the chicken genome was obtained by searching https://www.ncbi.nlm.nih.gov. The NCBI reference number was NP_001004406.1. The amino acid sequence for cofilin-2 was:

[SEQ ID NO: 1] MASGVTVNDEVIKVFNDMKVRKSSTPEEIKKRKKAVLFCLSDDKKQIIV EEATRILVGDIGDTVEDPYTAFVKLLPLNDCRYALYDATYETKESKKED LVFIFWAPESAPLKSKMIYASSKDAIKKKFTGIKHEWQVNGLDDIKDRS TLGEKLGGNVVVSLEGKPL

Codon Optimization of Cofilin-2

The amino acid sequence was codon optimized for expression in S. cerevisiae using ATUM's GeneGPS™ algorithm.

The codon optimized sequence for the chicken cofilin-2 gene was:

[SEQ ID NO: 2] ATGGCATCAGGCGTCACAGTGAACGATGAAGTTATCAAGGTTTTCAACG ATATGAAAGTTCGTAAGTCTAGCACCCCAGAGGAAATCAAAAAGAGAAA AAAAGCTGTCTTGTTTTGTTTATCCGATGACAAAAAGCAGATTATTGTA GAGGAAGCTACTAGAATCCTTGTGGGTGATATAGGTGACACTGTAGAGG ATCCTTACACTGCCTTCGTCAAGTTGTTACCATTAAATGATTGCAGATA TGCTCTCTACGACGCTACATACGAAACCAAGGAATCTAAAAAAGAGGAC TTGGTTTTCATCTTTTGGGCCCCTGAATCCGCGCCACTGAAGAGTAAGA TGATATACGCATCTTCAAAGGATGCAATTAAAAAAAAGTTCACAGGTAT TAAGCATGAATGGCAAGTTAACGGGCTTGATGATATTAAAGATAGATCT ACATTGGGTGAAAAGCTAGGCGGAAATGTTGTGGTTTCATTGGAAGGAA AGCCACTATAA

Cloning of Cofilin-2 Gene

The gene was synthesized by ATUM and cloned into the pD1248 (ATUM, Newark, Calif.) expression vector, which is a yeast integrating plasmid. The resulting plasmid was designated as (“pBOND4”). The gene was amplified using the cloning primers oBOND11 oBOND12 (see Table 11).

The resulting PCR fragment was digested with restriction enzymes XhoI and EcoRI, gel purified, and then ligated with T4 DNA ligase into the pRS424 (ATCC® 77105™) expression vector, which was linearized with the same restriction enzymes and dephosphorylated by Quick CIP (New England Biolabs). This generated the expression vector (“pBOND21”) which has a 2-micron origin of replication and can be selected by complementation of tryptophan auxotrophy.

The pBOND21 expression vector was introduced into an S. cerevisiae host cell ATCC®208288™ designated as (“sBOND1”) by transformation using Zymo Research™ Frozen-EZ Yeast Transformation II kit following the manufacturer's instructions. The empty vector (“pBOND8”) was transformed into the sBOND1 strain and ran in parallel as a control.

Cell Culture

Cells were grown in flasks on selective media (lacking tryptophan) containing 2% (w/v) raffinose until they reached an OD600 of 1 (i.e., exponential phase). During the exponential growth phase, galactose was added to the flask at a final concentration of 2% (w/v) to induce the expression of the cofilin-2 protein. After induction, the cultures were grown for another 24 hours with vigorous shaking.

Protein Analysis

Cells were collected by centrifugation. Cell pellets were weighed, and protein extracts were prepared using the Thermo Scientific™ YPER Yeast Protein Extraction Reagent according to manufacturer's instructions. The protein extracts were quantitated using the Pierce™ BCA Protein Assay Kit according to manufacturer's guidelines. Equal amounts of total protein were loaded for each lane and then analyzed by SDS-PAGE. Proteins were visualized by Coomassie staining.

Results

FIG. 1 is a photograph of the SDS-PAGE gel. The size of the cofilin-2 protein is 19 kDa. Lane 1 shows the molecular weight marker with the kDa sizes indicated. Lane 2 shows the host cell (sBOND1) with the empty vector (pBOND8), a control. Lane 3 shows a first clone (clone 1) of the host cell (sBOND1) with the pBOND21 vector. Lane 4 shows a second clone (clone 2) of the host cell (sBOND1) with pBOND21 vector.

We observed a 19 kDa protein strongly expressed in lane 3 and lane 4, indicating that two different clones of the S. cerevisiae host cell comprising the pBOND21 vector express the cofilin-2 protein. In contrast, no 19 kDa protein was observed in the control empty vector strain (lane 2). These results demonstrate that a chicken cofilin-2 gene can be robustly produced in an S. cerevisiae host cell.

6.9.9. Example 9: The Expression of a Recombinant Chicken Cofilin-2 Protein Did not Hinder the Growth of Saccharomyces cerevisiae

Overexpression of actin and actin binding-proteins (also referred to as the “actin cytoskeleton machinery”) has deleterious effects in eukaryotic cells, such as yeast. These deleterious effects can include lethality, slow growth rates (e.g., delayed progression through the cell cycle), and abnormal morphology (e.g., filamentous growth). See, Yoshikawa et al. (2011) Yeast 28: 349-361; Stevenson et al. (2001) PNAS 98(7): 3946-3951. Cofilins are actin binding proteins that drive depolymerization of actin filaments. See Winder and Ayscough, J. Cell Science (2005) 118 (4): 651-654.

This study was conducted to determine if overexpressing a chicken cofilin-2 gene in an S. cerevisiae host cell hinders the growth of the host cell.

Methods Cell Culture

The strains and cell culture were as described in Example 8.

Growth Study

The two flasks, for each strain, were grown in either raffinose only or raffinose plus galactose to induce induction of protein expression, yielding eight separate flasks (four of each strain). Samples were taken every hour for the first 10 hours and then at various subsequent time points. The OD600 was measured at each time point. The OD600 values were graphed as averaged values from two flasks.

The results of the growth curves are shown in FIG. 2 . The growth curves were established by plotting the average OD600 measurement over time. The maximum specific growth rate (μmax) was calculated by plotting ln(OD600) versus duration and then performing a linear regression analysis in Excel [version 16.31] from samples taken at the exponential phase. The value of μmax was determined by taking the maximum value of the slope between three time points. FIG. 3 shows the maximum specific growth rates (μmax [h-1]).

Results

We observed no significant difference in the final cell densities (e.g., growth curve at saturation) between the recombinant yeast strain expressing cofilin-2 and the empty vector control strain, using a 95% confidence statistical threshold (one-way ANOVA). See FIG. 2 . In addition, we observed no significant difference in the maximum specific growth rates between the recombinant yeast strains expressing cofilin-2 and the empty controls, using a 95% confidence statistical threshold (one-way ANOVA). See. FIG. 3 . Moreover, there was no significant difference in μmax for the cofilin-2 strain with or without galactose (with/or without induction of protein expression).

These results demonstrate that an S. cerevisiae host cell expressing cofilin-2 can effectively grow and therefore efficiently produce an animal muscle protein.

6.9.10. Example 10: Production of Recombinant Profilin Protein from Chicken in an Saccharomyces cerevisiae Host Cell

This study was conducted to determine if a chicken profilin gene can be recombinantly produced in an S. cerevisiae host cell.

Methods

Identification of Profilin Gene from Chicken

The sequence of chicken profilin was identified by searching Uniprot.org. The UniProt accession number was Q5ZL50. The amino acid sequence was:

[SEQ ID NO: 3] MAGWQSYVDNLMCDGCCQEAAIVGYCDAKYVWAATAGGIFQSITPVEID MIVGKDREGFFTNGLTLGAKKCSVIRDSLYVDGDCTMDIRTKSQGGEPT YNVAVGRAGRVLVFVMGKEGVHGGGLNKKAYSMAKYLRDSGF

Codon Optimization of Profilin

The amino acid sequence was codon optimized for expression in S. cerevisiae using ATUM's GeneGPS™ algorithm. The resulting gene sequence was:

[SEQ ID NO: 4] ATGGCTGGCTGGCAATCTTATGTGGATAACTTAATGTGTGATGGATGTT GTCAAGAGGCTGCAATCGTGGGTTACTGCGACGCAAAATACGTTTGGGC AGCAACAGCTGGGGGCATATTCCAATCAATTACACCAGTTGAAATTGAT ATGATCGTTGGTAAAGATAGAGAGGGATTTTTCACTAATGGTCTAACTT TAGGTGCCAAAAAGTGCAGTGTTATCAGAGACTCACTGTACGTAGACGG GGATTGCACCATGGATATTCGTACAAAGTCTCAGGGTGGAGAACCTACA TACAACGTCGCGGTCGGCAGAGCCGGGAGAGTTTTGGTTTTCGTAATGG GCAAGGAAGGTGTCCATGGTGGTGGACTTAACAAAAAGGCCTACTCTAT GGCTAAGTACTTGAGAGATTCCGGTTTTTAA

Cloning of Profilin Gene

The gene was synthesized by ATUM and cloned into the pD1205 (ATUM) expression vector, which is a 2-micron episomal vector that has GAL1-promoter and the TRP1 selection marker gene. The resulting expression vector was designated as (“pBOND3”).

The vector was transformed into chemically competent E. coli strain 5-alpha (New England Biolabs) by heat-shock transformation following the manufacturer's protocol and selection on Luria Bertani (LB) agar plates containing 25 μg/mL chloramphenicol.

Colonies were patched onto fresh LB plates with chloramphenicol and incubated at 37° C. Liquid cultures were inoculated and grown with shaking until saturation. The expression vector was purified using Zyppy plasmid miniprep kit (Zymo Research) by following the manufacturer's instructions.

The gene insert was verified by PCR and restriction digestion and transformed into host cell (“sBOND1”). The pBOND3 expression vector was transformed into the sBOND1 host cell by electroporation in a Bio-Rad Gene Pulser™.

The cell suspension was spread onto tryptophan dropout selection plates containing 6.8 g/L yeast nitrogen base without amino acids (Sigma Y0626), 1.9 g/L yeast synthetic dropout medium without tryptophan (Sigma Y1876), and 2% glucose (w/v).

Cell Culture

Cells were grown in medium containing 6.8 g/L yeast nitrogen base without amino acids (Sigma Y0626), 1.9 g/L yeast dropout supplements without tryptophan (Sigma Y1876) and 20 g/L raffinose. The strain was cultured to an OD600 of 1, at which time profilin expression was induced by adding 20 g/L galactose. After induction, the culture was grown for an additional 18 hours. The final OD600 was around 8.

Protein Analysis

Samples were taken at time of induction, after 5 hours, and at the end of the cultivation. Protein cell extracts were made as follows. The cells were lysed by a sodium hydroxide protocol (Kushnirov, 2000, Rapid and reliable protein extraction from yeast. Yeast, 16, 857-860). Cell pellets from 0.5 mL cell suspension were resuspended in 100 μL deionized water and 100 μL 0.2 N NaOH was added to each tube and then incubated at room temperature for 5 min. Subsequently, the cells were spun down for 1 min at 16000 g and resuspended in 40 μL sample buffer containing SDS (Laemmli, 1970, Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4. Nature, 227, 680-685).

Equal amounts of total protein were loaded to each lane and analyzed by SDS-PAGE. Proteins were visualized by Coomassie staining, see FIG. 4 .

Results

FIG. 4 shows the results from the SDS-PAGE separation. The size of the profilin protein is 15 kDa. Lane 1 shows the molecular weight marker. Lane 2 shows protein expression before induction. Lane 3 shows protein expression 5 hours after induction by galactose. Lane 4 shows protein expression 18 hours after induction.

We observed increasing intensity of a 15 kDa protein band in lanes 3-4, where the S. cerevisiae host cell was induced to express the chicken profilin protein at increasing time durations. In contrast, a 15 kDa protein was not detected where there was no induction, lane 2. These results demonstrate that a chicken profilin protein can be produced in an S. cerevisiae host cell.

6.9.11. Example 11: The Expression of a Recombinant Chicken Profilin Protein Did not Severely Hinder the Growth of an Saccharomyces cerevisiae Host Cell

This study was conducted to determine if a recombinant S. cerevisiae host cell overexpressing a chicken profilin gene hinders the growth of the host cell.

Methods Cell Culture

Cells were grown in flasks, two flasks for each strain, in either raffinose only or raffinose plus galactose, to induce induction of protein expression, yielding eight separate flasks (four for each strain). The medium also contained 6.8 g/L yeast nitrogen base without amino acids (Sigma Y0626) and 1.9 g/L yeast synthetic dropout medium without tryptophan (Sigma Y1876). The cultures were inoculated at an OD600 of about 0.2, and then grown for approximately 30 hours. Samples were taken every hour for the first 10 hours and then at various subsequent time points. The OD600 was measured at each time point. The OD600 values were graphed as averaged values from two flasks.

Growth Analysis

Samples were taken every hour for the first 10 hours and then at various subsequent time points. The growth analysis was conducted as described in the Example 9.

Results

The results from the growth study are shown in FIG. 6 and FIG. 7 . The growth curves are shown in FIG. 6 . The maximum specific growth rates are shown in FIG. 7 . We observed no significant differences between the final OD600 values or the maximum specific growth rates, between the strains expressing recombinant profilin and the empty control strains, using a 95% confidence statistical threshold (ANOVA). However, the maximum specific growth rate for the profilin strain was significantly higher for the uninduced culture compared to when induced with galactose (P value <0.05). The final OD600 values were also significantly higher for the uninduced culture. Still, the difference in final cell density was only around 20%. Thus, these results demonstrate that the expression of a chicken profilin-2 gene does not severely hinder the growth of a S. cerevisiae host cell.

6.9.12. Example 12: Recombinantly Expressed Profilin Protein can Increase the Amount of Essential Amino Acids

This study was conducted to determine if the recombinantly expressed profilin protein can increase the percentage of essential amino acids in a whole-cell extract.

Methods

Identification of Profilin Gene from Chicken

The identification, cloning, and codon-optimization of the profilin gene were conducted as described in Example 10.

Cell Culture

Profilin was expressed in several shake flask cultivations (about 10 L total) grown in 20 g/l raffinose medium (tryptophan dropout medium, as above) and induced by 20 g/l galactose at an OD600 around 1. After induction, the culture was grown for additional 22-24 hours. A control culture containing pBOND8 was prepared in the same host cell and ran in parallel.

Amino Acid Analysis

The cells were concentrated by filtration using a 0.45 μm cellulose acetate membrane. The cells were dried at 65° C. for a minimum of 2 hours. The dried cells were submitted to Midwest laboratories for analysis.

Results

Table 2 shows the amino acid analysis of S. cerevisiae expressing profilin compared to a control without profilin. Values reported as % (w/w).

TABLE 2 Crude protein and amino acid analysis of S. cerevisiae expressing profilin compared to a control without profilin Relative shift compared to Analyte Profilin Control control Protein (crude) 53.4% 54.8% Aspartic acid 5.16% 4.15% 24% Threonine * 1.63% 1.38% 18% Serine 2.55% 2.07% 23% Glutamic acid 5.85% 5.41%  8% Proline 1.87% 1.55% 21% Glycine 2.26% 1.90% 19% Alanine 3.04% 3.37% −10%  Cystine 0.57% 1.00% −43%  Valine * 3.00% 2.82%  6% Methionine * 0.88% 0.71% 24% Isoleucine * 2.49% 2.11% 18% Leucine * 3.73% 3.40% 10% Tyrosine 1.82% 1.72%  6% Phenylalanine * 2.25% 1.87% 20% Lysine * 3.92% 3.62%  8% Histidine * 1.16% 1.23% −6% Arginine * 2.76% 2.69%  3% Tryptophan * 0.63% 0.54% 17% * indicates that the amino acid is an essential amino acid for dogs.

These results show the recombinant host cell expressing profilin had an increase in all but one of the essential amino acids analyzed, as determined by % of dry weight. The only exception was histidine. See, the column labeled “Relative shift compared to control” in Table 2.

These results demonstrate that a recombinantly expressed profilin protein can increase the amount of essential amino acids in a whole-cell extract.

6.9.13. Example 13: Treat Composition Made with Recombinant Animal Protein

This study was conducted to determine if the recombinant animal protein powder can be used in a recipe with other ingredients to produce a food composition in the form of a dried pet treat.

Methods

Identification of Profilin Gene from Chicken

The identification, cloning, and codon-optimization of the profilin gene were conducted as described in Example 10.

Cell Culture

Cells were grown in flasks, in 13 L of 20 g/l raffinose medium, 6.8 g/L yeast nitrogen base without amino acids (Sigma Y0626), and 1.9 g/L yeast synthetic dropout medium without tryptophan (Sigma Y1876). When the culture reached an OD600 of 1 (i.e., exponential phase growth), expression of the profilin protein was induced by adding 20 g/l galactose. After induction, the culture was grown for another 22-24 hours, yielding a culture with an OD600 range of approximately 7-9.

The resulting cells were concentrated by filtration using a 0.45 μm cellulose acetate membrane. Next, the cells were dried at 70° C. for 1.5 hours. The dry weight yield was approximately 2.5 g/L.

Processing into a Treat

Whole-cells expressing the chicken profilin protein were pelleted and dried as described above. The dried pellets are shown in FIG. 8 . Next, the pellets were ground until they became a fine powder. The profilin protein powder was then mixed with other ingredients, as outlined in Table 3, to make three different formulations. Each formulation contained different amounts of the profilin protein.

TABLE 3 Ingredients used for dog treats with recombinant chicken profilin protein Ingredient (g) Ingredient (wt %) Ingredient Treat #1 Treat #2 Treat #3 Treat #1 Treat #2 Treat #3 Red lentil flour 5.5 5.5 5.5 9.2% 8.4% 9.9% Sweet potato puree 12.8 12.8 12.8 21.3% 19.4% 23.0% Chickpea flour 7.7 7.7 7.7 12.8% 11.7% 13.8% Coconut oil 5.1 5.1 5.1 8.5% 7.8% 9.2% Oat flour 5.1 5.1 5.1 8.5% 7.8% 9.2% Quick oats 2.6 2.6 2.6 4.3% 3.9% 4.6% Pea protein 5.1 5.1 5.1 8.5% 7.8% 9.2% Cinnamon 0.024 0.024 0.024 0.04% 0.04% 0.04% Molasses 4.4 4.4 4.4 7.3% 6.6% 7.8% Water 1.4 1.4 1.4 2.4% 2.2% 2.5% S. cerevisiae containing 10.3 (5.5) 16.25 (8.7) 6.0 (3.2) 17.1% (9.1%) 24.6% (13.2%) 10.8% (5.8%) chicken profilin (protein contribution from S. cerevisiae)

The treat was produced using the following method. The dry ingredients were mixed in a bowl. See FIG. 9 . Next, the dry and wet ingredients were added to an electric mixer and mixed until the ingredients formed a dough-like consistency. Then the dough was compacted into the mold using a rolling pin. See FIG. 10A. The mold made a perforated pattern into the dough so that individual pieces can be broken off. See FIG. 10B. Finally, the treat was baked for 30 min at 250° F., and then dehydrated for 12 hours at 90° F.

Results

Three dog treats containing different amounts of the recombinant chicken profilin protein (3 grams, 5 grams, and 8 grams) were made. See FIGS. 11A-C. Taken together, the examples above demonstrate that the recombinant chicken profilin protein can be used to make treat composition that has a higher amount of essential amino acids.

6.9.14. Example 14: Production of Recombinant Coronin Protein from Chicken in an Saccharomyces cerevisiae Host Cell

This study was conducted to determine if an S. cerevisiae host cell could express a coronin protein from chicken.

Coronin has been classified as a side-binder and signaling protein. See Winder and Ayscough, J. Cell Science (2005) 118(4): 651-654. The particular coronin used here (coronin 6) is muscle-specific.

Methods

Identification of the Coronin Gene Sequence from a Chicken Genome

The sequence of chicken coronin was obtained by searching Uniprot.org. The UniProt accession number was F1NXA5. The amino acid sequence was:

[SEQ ID NO: 5] MSRRVVRQSKFRHVFGQPVKADQMYEDIRVSKVTWDSSFCAVNPKFVAI IVEAGGGGAFMVLPLAKTGRVDKNHPLVTGHTAPVLDIDWCPHNDNVIA SASEDTTVMVWQIPDYVPVRSITEPVVTLEGHSKRVGIICWHPTARNVL LSAGCDNLVILWNVGTGEMLLALEDMHTDLIYNVGWNRNGSLLVTTCKD KKVRVIDPRKQTVVAEITKPHDGARPIRAIFMADGKIFTTGFSKMSERQ LGLWDLKNFEEPIALQEMDTSNGVLLPFYDPDTNIVYLCGKGDSSIRYF EITDEAPYVHYLNTYSSKEPQRGMGFMPKRGLDVSKCEIARFFKLHERK CEPIVMTVPRKSDLFQDDLYPDTPGPEPALEADEWLSGKDAEPILISLR DGYVPVKNRELKVVKKNILDSKPPPGPRRSHSTSNTDISTPALDEVLEE IRVLKETVQAQEKRISALEHKLCQFTNGTD

Codon Optimization of Coronin

The amino acid sequence was codon optimized for expression in S. cerevisiae using ATUM's GeneGPS™ algorithm. The resulting gene sequence was:

[SEQ ID NO: 6] ATGTCTCGTAGAGTTGTTAGACAATCCAAGTTCCGTCACGTGTTCGGCC AACCAGTTAAGGCAGATCAGATGTACGAAGATATCAGAGTTTCAAAGGT TACCTGGGACTCATCTTTTTGCGCTGTTAACCCAAAGTTCGTAGCAATA ATTGTGGAAGCTGGCGGTGGGGGAGCATTTATGGTTTTACCACTAGCCA AGACTGGTAGAGTCGACAAAAATCACCCTTTAGTCACTGGACATACAGC ACCTGTATTAGATATTGACTGGTGTCCACATAACGACAATGTTATTGCA AGTGCATCTGAGGATACAACTGTCATGGTATGGCAAATCCCAGACTACG TTCCAGTAAGATCAATCACAGAACCAGTTGTCACGCTCGAGGGTCACTC TAAGAGAGTTGGCATTATCTGTTGGCATCCTACAGCCAGAAATGTGTTG TTGTCTGCCGGTTGCGATAACTTGGTAATTCTTTGGAACGTCGGTACAG GCGAAATGTTGCTGGCGCTTGAAGATATGCACACTGACCTCATTTACAA CGTCGGATGGAACAGAAACGGGTCGTTATTAGTCACCACATGTAAAGAT AAAAAGGTAAGGGTTATCGACCCTAGAAAGCAAACAGTTGTTGCGGAAA TCACAAAGCCACATGATGGTGCTAGACCAATTAGAGCTATATTCATGGC CGATGGTAAGATTTTCACAACCGGATTCTCAAAAATGTCCGAGAGACAA CTTGGGTTGTGGGATCTTAAAAACTTCGAGGAACCAATTGCTCTGCAGG AAATGGATACTAGTAATGGTGTTTTGTTACCATTTTACGACCCAGACAC AAACATCGTTTACCTCTGCGGCAAGGGTGATAGTAGCATCAGATATTTT GAGATAACAGATGAAGCTCCTTACGTCCATTACTTGAATACTTACTCCT CAAAGGAACCACAGAGAGGTATGGGATTCATGCCAAAGCGAGGACTAGA TGTTTCTAAGTGTGAAATCGCTAGATTTTTCAAGTTACATGAGAGAAAA TGCGAACCTATTGTGATGACAGTGCCTAGAAAATCTGATTTGTTCCAAG ATGATCTATATCCAGATACTCCTGGCCCAGAACCAGCCCTTGAAGCTGA TGAATGGTTATCTGGTAAAGATGCAGAGCCAATACTAATTTCTCTTAGA GATGGGTACGTCCCAGTGAAAAACAGAGAGTTGAAAGTTGTTAAAAAAA ATATTTTGGATAGCAAGCCTCCTCCAGGTCCTCGTAGATCTCACTCCAC ATCAAACACCGATATATCAACACCAGCTTTGGATGAAGTTTTAGAGGAA ATCCGGGTGTTGAAGGAAACTGTACAAGCACAAGAGAAGAGAATCTCAG CACTGGAACATAAGCTATGTCAATTTACTAATGGTACCGACTAA

Cloning of Coronin Gene

The gene was synthesized by ATUM and cloned into the pD1205 vector (ATUM), which is a 2-micron episomal vector that has a GAL1-promoter and the TRP1 selection marker gene. This expression vector was designated as (“pBOND2”).

The pBOND2 expression vector was transformed into chemically competent E. coli strain 5-alpha (New England Biolabs) by heat-shock transformation following the manufacturer's protocol. Selection for transformation was conducted on LB agar plates containing 25 μg/mL chloramphenicol. Colonies were patched on fresh LB agar plates with chloramphenicol and grown in liquid LB with 25 μg/mL chloramphenicol at 37° C. until saturation. The expression vector was purified using the Zyppy plasmid miniprep kit (Zymo Research) following the manufacturer's instructions. The gene insert was verified by PCR and restriction digestion. The expression vector was transformed into S. cerevisiae strain sBOND1 strain by electroporation using a Bio-Rad Gene Pulser™.

The cell suspension was spread onto selection plates comprising dropout tryptophan plates containing 6.8 g/L yeast nitrogen base without amino acids (Sigma Y0626), and 1.9 g/L yeast synthetic dropout medium without tryptophan (Sigma Y1876), and 2% glucose).

Cell Culture

Cells were grown in a medium containing 6.8 g/L yeast nitrogen base without amino acids (Sigma Y0626), 1.9 g/L yeast dropout supplements without tryptophan (Sigma Y1876) and 20 g/L raffinose, until the culture reached an OD600 of 1, at which time coronin expression was induced by adding 20 g/L galactose (from a sterile filtered 40% (w/v) solution). After induction, cells were grown for an additional 18 hours. At the end of induction, the OD600 was around 8.

Protein Analysis

Samples were taken at the time of induction, after 5 hours after induction, and at the end of the cultivation. The cells were lysed as described in Example 10. Equal amounts of total protein were loaded onto each lane and analyzed by SDS-PAGE using a precast SDS-PAGE gel (MiniProtean TGX, 4-20% gradient, Bio-Rad). Proteins were visualized by Coomassie staining, see FIG. 12 .

Results

FIG. 12 shows the results of the SDS-PAGE analysis. The size of coronin is 53 kDa. Lane 1 shows the molecular weight marker. Lane 2 shows protein expression before induction. Lane 3 shows protein expression 5 hours after induction. Lane 4 shows protein expression 18 hours after induction.

Lanes 2-4 show an increasing amount of a 53 kDa protein, the expected size of codon optimized coronin protein. These results demonstrate that a chicken coronin protein can be produced in a S. cerevisiae host cell.

6.9.15. Example 15: Production of Recombinant Myozenin-1 Protein from Turkey in an Saccharomyces cerevisiae Host Cell

This study was conducted to determine if an S. cerevisiae host cell could express a myozenin-1 protein from turkey.

Myozenins function as calcineurin-interacting proteins that help tether calcineurin to the sarcomere of cardiac and skeletal muscle. They play an important role in modulation of calcineurin signaling. Myozenin 1 is predominantly expressed in fast-twitch skeletal muscle.

Methods:

Identification of the Myozenin-1 Gene Sequence from a Turkey Genome

The sequence of turkey myozenin-1 was obtained by searching https://www.ncbi.nlm.nih.gov. The NCBI Reference number was XP_010712691.1. The amino acid sequence was:

[SEQ ID NO: 7] MPLAGTPAPLKRKKPTKLIGKLTHEVMPQEVTKLNLGKKISIPRDVMLE ELSLLTNKGSKMFKLRQLRVEKFIYENNPDAFSDNSVDHFQRFIPSGGH YGEDAHGYGHGRMVGGVTAGQHGSSKQHYSTVPPRPGSKGGPGNSEGEH EAEKSAGSAGGGHGTEKDGKSGGKKPLLKTYISPWERAMGISPEDKSQL TIDLLSYSPKADFPHYKSFNRTAMPYGGYEKAAKRMTFKVPQFDICPLL PESIVLYNQNFRNRPSFNRTPIPWMPSGESSEYHTDINVPRSGETEEL

Codon Optimization of Myozenin-1

The amino acid sequence was codon optimized for expression in S. cerevisiae using ATUM's GeneGPS™ algorithm. The resulting gene sequence was:

[SEQ ID NO: 8] ATGCCTTTAGCCGGAACCCCAGCACCATTGAAGAGAAAAAAGCCAACAA AACTTATTGGTAAGCTGACACACGAAGTTATGCCACAGGAAGTTACCAA GTTGAATCTAGGTAAAAAGATTTCTATCCCTAGAGATGTCATGTTGGAA GAGTTATCGTTATTGACGAACAAAGGTTCCAAAATGTTCAAGTTGAGAC AATTAAGAGTCGAGAAATTCATTTACGAAAACAATCCAGACGCATTCTC CGATAACAGTGTTGATCATTTTCAACGTTTTATCCCATCTGGTGGACAT TATGGTGAAGATGCCCATGGGTACGGTCATGGTCGTATGGTTGGGGGCG TTACAGCCGGGCAACATGGTTCATCAAAGCAACATTACAGTACCGTGCC TCCTCGACCTGGTTCTAAGGGTGGTCCAGGTAACTCTGAGGGTGAACAT GCTGAAAAGTCAGCTGGGTCTGCTGGAGAGGGCGGCCACGGTACAGAAA AGGATGGTAAGAGTGGTGGCAAAAAGCCTCTACTTAAGACTTACATCAG CCCATGGGAGAGAGCGATGGGAATCTCACCAGAGGATAAGAGCCAGTTA ACTATTGATCTTCTATCATATTCACCAAAGGCAGACTTCCCACACTACA AATCTTTTAACAGAACAGCAATGCCATACGGCGGATACGAAAAAGCTGC TAAGAGAATGACATTTAAGGTACCTCAATTCGATATCTGTCCACTGTTG CCAGAATCCATAGTACTCTACAACCAAAATTTCAGAAACAGACCATCAT TCAATAGAACTCCTATACCTTGGATGCCATCTGGCGAATCTTCCGAATA CCACACTGACATTAACGTGCCAAGATCTGGAGAAACAGAGGAATTGTAA

Cloning of Myozenin-1

The gene was synthesized by ATUM and cloned into the pD1211 (ATUM) vector, which is a yeast episomal plasmid, containing a 2-micron origin of replication, and the LEU2 selection marker. Expression of turkey myozenin-1 was driven by a yeast TEF1 promoter. This expression vector was designated as (“pBOND11”).

The pBOND11 expression vector was introduced into the host cell, S. cerevisiae ATCC® MYA-1108™ designated as (“sBOND28”) by transformation using Zymo Research™ Frozen-EZ Yeast Transformation II kit following the manufacturer's instructions. Transformants were selected using synthetic complete medium lacking leucine.

Cell Culture

Cells were grown in flasks with selective media lacking leucine, containing 20 g/l glucose until the culture reached saturation, at which point the cells were collected by centrifugation.

Protein Analysis

Cell pellets were weighed, and protein extracts were prepared using the Thermo Scientific™ YPER Yeast Protein Extraction Reagent according to manufacturer's instructions. Yeast extracts were quantitated using the Pierce™ BCA Protein Assay Kit according to manufacturer's guidelines. Equal amounts of total protein were loaded on each lane analyzed by SDS-PAGE. Proteins were visualized by Coomassie staining, see FIG. 13 .

Results

FIG. 13 shows a photograph of the SDS-PAGE gel after staining. The molecular size of myozenin-1 is 32 kDa. Lane 1 shows the molecular weight marker. Lane 2 shows the host cell (sBOND28). Lane 3 shows the host cell (sBOND28) with pBOND11 expressing myozenin-1 protein (clone 1). Lane 4 shows the host cell (sBOND28) with pBOND11 expressing myozenin-1 protein (clone 2).

We observed an increased expression of a 32 kDa protein with the clones expressing myozenin-1 protein (lanes 3-4), compared to the empty vector control strain (lane 2). These results demonstrate that an S. cerevisiae host cell could robustly produce a turkey myozenin-1 protein.

6.9.16. Example 16: Production of Recombinant Troponin C Protein from Pig in an Saccharomyces cerevisiae Host Cell

This study was conducted to determine if an S. cerevisiae host cell could express a troponin C protein from pig.

Troponin C is a protein that resides in the troponin complex on actin thin filaments of striated muscle and is responsible for binding calcium to activate muscle contraction.

Methods:

Identification of the Troponin C Gene Sequence from a Pig Genome

The sequence of pig troponin C, skeletal muscle, was obtained by searching https://www.ncbi.nlm.nih.gov. The NCBI reference number NP_001001862.1. The amino acid sequence was:

[SEQ ID NO: 9] MTDQQAEARSYLSEEMIAEFKAAFDMFDADGGGDISVKELGTVMRMLGQ TPTKEELDAIIEEVDEDGSGTIDFEEFLVMMVRQMKEDAKGKSEEELAE CFRIFDRNADGYIDAEELAEIFRASGEHVTDEELESLMKDGDKNNEGRI DFDEFLKMMEGVQ

Codon-Optimization of Troponin C

The amino acid sequence was codon-optimized for expression in S. cerevisiae using ATUM's GeneGPS™ algorithm. The resulting gene sequence was:

[SEQ ID NO: 10] ATGACTGATCAACAAGCTGAAGCAAGATCTTACCTTAGTGAAGAGATGA TAGCAGAGTTTAAGGCAGCGTTCGATATGTTCGATGCCGACGGTGGTGG CGATATCTCTGTGAAGGAACTCGGTACAGTTATGAGAATGCTGGGGCAA ACACCAACCAAGGAAGAGTTGGATGCAATCATCGAAGAGGTCGACGAAG ATGGGTCAGGTACAATTGATTTTGAAGAGTTTTTGGTTATGATGGTAAG ACAGATGAAAGAGGATGCTAAGGGTAAGTCAGAAGAGGAATTAGCTGAA TGTTTTAGAATTTTCGATAGAAATGCTGATGGATACATTGACGCTGAGG AACTAGCCGAAATTTTCCGTGCCTCTGGAGAACATGTCACTGATGAGGA ATTGGAATCCTTAATGAAAGATGGCGACAAAAACAACGAGGGTAGAATC GACTTCGACGAATTCCTTAAGATGATGGAAGGCGTTCAATAA

The gene was synthesized by ATUM and cloned into the pD1205 (ATUM) vector, which is a 2-micron episomal vector that has GAL1-promoter and the TRP1 gene to allow selection when transformed into a strain with tryptophan auxotrophy. The resulting expression vector was designated (“pBOND19”).

Transformation of pBOND19 into the S. cerevisiae host cell (“sBOND1”) was carried out using Zymo Research™ Frozen-EZ Yeast Transformation II kit following the manufacturer's instructions and selecting on synthetic complete media lacking tryptophan. An empty vector control (pBOND8) strain was prepared using the same host cell.

Cell Culture

Cells were grown in flasks on selective media (lacking tryptophan) containing 2% (w/v) raffinose until the culture reached an OD600 of 1. Galactose was added to a final concentration of 2% (w/v) to induce expression of the protein. After induction, cultures were grown for another 24 hours with vigorous shaking.

Protein Analysis

Cell pellets were weighed, and protein extracts were prepared using the Thermo Scientific™ YPER Yeast Protein Extraction Reagent according to manufacturer's instructions. Samples were quantitated using the Pierce™ BCA Protein Assay Kit according to the manufacturer's guidelines. Equal amounts of total protein were loaded onto each lane and analyzed by SDS-PAGE. Proteins were visualized by Coomassie staining, see FIG. 14 .

Results

FIG. 14 shows the stained SDS-PAGE gel. The molecular size of troponin C is 18 kDa. Lane 1 shows a molecular weight marker. Lane 2 shows the host cell (sBOND1) with an empty vector (pBOND8). Lane 3 shows the host cell (sBOND1) with pBOND19 expressing the troponin C protein (clone 1). Lane 4 shows the host cell (sBOND1) with pBOND19 expressing the troponin C protein (clone 2).

We observed an 18 kDa protein in lanes 3-4. Notably, there was no such 18 kDa protein observed in the empty control strain (lane 2). These results demonstrate that a S. cerevisiae host cell could produce a pig troponin C protein.

6.9.17. Example 17: Production of Recombinant Cofilin-2 from Chicken in a Komagataella phaffii Host Cell

This study was conducted to determine if a Komagataella phaffii (formerly known as Pichia pastoris) host cell could express a cofilin-2 protein from chicken.

Methods:

Identification of the Cofilin-2 Gene Sequence from a Chicken Genome

The sequence of chicken cofilin-2 was obtained by searching https://www.ncbi.nlm.nih.gov. The NCBI reference number was NP_001004406.1. The amino acid sequence was [SEQ ID NO: 1].

Codon Optimization of Cofilin-2

The amino acid sequence was codon optimized for expression in K. phaffii using ATUM's GeneGPS™ algorithm. The resulting gene sequence was:

[SEQ ID NO: 11] ATGGCTTCTGGTGTGACTGTTAACGACGAAGTCATCAAGGTATTCAATG ATATGAAAGTTAGAAAATCATCCACTCCAGAGGAAATCAAAAAGAGAAA AAAAGCCGTTCTATTTTGCCTGTCGGACGACAAAAAGCAGATCATCGTT GAGGAAGCCACACGTATTTTGGTCGGTGACATTGGTGACACAGTCGAAG ATCCTTATACTGCTTTTGTTAAGCTGTTGCCCTTAAATGATTGTAGGTA CGCTCTGTACGACGCAACTTACGAAACCAAAGAGTCCAAAAAAGAGGAT TTGGTGTTCATCTTCTGGGCACCTGAAAGTGCTCCACTTAAGAGCAAGA TGATTTATGCATCCTCTAAAGATGCTATTAAAAAAAAGTTTACAGGTAT AAAGCATGAGTGGCAAGTGAACGGATTGGATGACATTAAAGATAGATCT ACGTTGGGCGAAAAGCTTGGTGGAAATGTTGTAGTGTCATTAGAGGGAA AGCCACTCTAA

Cloning of Cofilin-2

The gene was synthesized by ATUM and cloned into the pD902 vector (ATUM), which is a yeast integrating plasmid that has a zeocin resistance gene for selection, and an AOX1 promoter. The resulting expression vector was designated as (“pBOND24”).

Komagataella phaffii (formerly Pichia pastoris) PPS-9016 was obtained from ATUM and designated as (“sBOND2”). The pBOND24 expression vector was linearized using the restriction enzyme PmeI and was introduced into the sBOND2 host cell by transformation using Zymo Research™ Frozen-EZ Yeast Transformation II kit following the manufacturer's instructions. Cells were allowed to recover overnight in non-selective media, and the following day they were plated on selective plates containing YPD (10 g/l yeast extract, 20 g/l peptone, 20 g/l glucose) with either 250 μg/ml or 1000 μg/ml zeocin.

Cell Culture

The cells were grown in baffled flasks in BMGY plus zeocin media (10 g/l yeast extract, 20 g/l peptone, 13.4 g/L yeast nitrogen base (without amino acids), 100 mM potassium phosphate pH 6, 0.004 mg/L biotin, 1% (v/v) glycerol, and 500 μg/ml zeocin) until the culture reached an OD600 of 1. Methanol was added to a final concentration of 0.5% (v/v) to induce expression of the protein. After induction, cultures were grown for another 60 hours with vigorous shaking, and methanol was added every 24 hours to maintain and/or boost induction of protein expression.

Protein Analysis

Cell cultures were collected by centrifugation and cell pellets were weighed. Protein extracts were prepared using the Thermo Scientific™ YPER Yeast Protein Extraction Reagent according to the manufacturer's instructions. Yeast extracts were quantitated using the Pierce™ BCA Protein Assay Kit according to the manufacturer's guidelines. Equal amounts of total protein were loaded to each lane and analyzed by SDS-PAGE. Proteins were visualized by Coomassie staining, see FIG. 15 .

Results

FIG. 15 shows the results of the SDS-PAGE gel. The molecular size of cofilin-2 is 19 kDa. Lane 1 shows the molecular weight marker. Lane 2 shows the host cell (sBOND2). Lane 3 shows the host cell (BOND2) with pBOND24 expressing the cofilin-2 protein (clone #2). Lane 4 shows the host cell (sBOND2) with pBOND24 expressing the cofilin-2 protein (clone #3). Lane 5 shows the host cell (sBOND2) with pBOND24 expressing the cofilin-2 protein (clone #5).

We observed a strong band at 19 kDa in the cofilin-2 expressing clones (lanes 3-5), while no such band was observed in the control lane (lane 2). These results demonstrate that a Komagataella phaffii host cell could robustly produce a chicken cofilin-2 protein.

6.9.18. Example 18: Production of Recombinant Profilin Protein from Chicken in a Komagataella phaffii Host Cell

This study was conducted to determine if a Komagataella phaffii host cell could express a profilin protein from chicken.

Methods

Identification of Profilin Gene Sequence from Chicken

The sequence of chicken profilin was obtained by searching Uniprot.org. The UniProt accession number is Q5ZL50. The amino acid sequence was: [SEQ ID NO:34]

Condon Optimization of Profilin

The amino acid sequence was codon optimized for K. phaffii using ATUM's GeneGPS™ algorithm. The resulting gene sequence was:

[SEQ ID NO: 12] ATGGCCGGTTGGCAATCCTATGTAGACAATCTAATGTGTGACGGGTGTT GCCAAGAGGCAGCAATTGTGGGTTACTGCGATGCTAAATACGTTTGGGC AGCAACAGCCGGCGGAATCTTCCAATCAATAACCCCAGTGGAAATTGAT ATGATTGTTGGAAAAGACCGTGAGGGATTTTTCACTAATGGTTTGACTC TGGGTGCTAAAAAGTGTTCCGTTATCCGTGATAGCTTGTATGTCGATGG TGACTGTACTATGGATATCAGGACAAAGTCGCAGGGTGGTGAACCTACG TATAACGTAGCTGTCGGTAGAGCTGGAAGAGTGTTAGTCTTTGTTATGG GTAAAGAGGGTGTTCATGGCGGTGGACTTAACAAAAAGGCTTACAGTAT GGCTAAGTACTTGAGAGACTCTGGATTCTAA

Cloning of Profilin

The gene was synthesized by ATUM and cloned into the pD902 vector, which is a yeast integrating plasmid that has a zeocin resistance gene to allow for the selection of transformants, and an AOX1 promoter. The resulting expression vector was designated (“pBOND25”).

Komagataella phaffii (formerly Pichia pastoris) PPS-9016 was obtained from ATUM and designated as (“sBOND2”). pBOND25 was linearized using the restriction enzyme, PmeI and was transformed into sBOND2 using the Zymo Research™ Frozen-EZ Yeast Transformation II kit following the manufacturer's instructions. Cells were allowed to recover overnight in non-selective media, and the following day they were plated on YPD agar plates containing 1000 μg/ml zeocin for selection.

Cell Culture

Cells were grown in flasks in BMGY plus zeocin media (10 g/l yeast extract, 20 g/l (w/v) peptone, 13.4 g/l yeast nitrogen base (without amino acids), 100 mM potassium phosphate pH 6.0, 0.004 mg/l biotin, 1% (v/v) glycerol, and 500 μg/ml zeocin) until the culture reached a OD600 of 1. Methanol was added to a final concentration of 0.5% (v/v) to induce expression of the protein. After induction, cultures were grown for an additional 60 hours with vigorous shaking. Methanol was added every 24 hours after the first time to maintain and/or boost induction of protein expression.

Protein Analysis

Cell cultures were collected by centrifugation and cell pellets were weighed. Protein extracts were prepared using the Thermo Scientific™ YPER Yeast Protein Extraction Reagent according to manufacturer's instructions. Yeast extracts were quantitated using the Pierce™ BCA Protein Assay Kit the according to manufacturer's guidelines. Equal amounts of total protein were loaded on each lane and analyzed by SDS-PAGE. Proteins were visualized by Coomassie staining, see FIG. 16 .

Results

FIG. 16 shows the results of the SDS-PAGE gel. The molecular size of profilin is 15 kDa. Lane 1 shows the molecular weight marker. Lane 2 shows the host cell (sBOND2). Lane 3 shows the host cell (sBOND2) with pBOND25 expressing the profilin protein (clone 1). Lane 4 shows the host cell (sBOND2) with pBOND25 expressing the profilin protein (clone 2). Lane 5 shows the host cell (sBOND2) with pBOND25 expressing the profilin protein (clone 3).

We observed a protein band at 15 kDa, in the profilin expressing clones (lanes 3-5), while no such band was observed in the empty control strain (lane 2). These results demonstrate that a Komagataella phaffii host cell could produce a chicken profilin protein.

6.9.19. Example 19: Production of Recombinant Profilin Protein from Chicken in a Kluyveromyces lactis Host Cell

This study was conducted to determine if a Kluyveromyces lactis host cell could express a profilin protein from chicken.

Methods

Identification and Cloning of Profilin Gene from Chicken

The identification and cloning of the profilin gene were carried out as described in Example 10.

The profilin gene [SEQ ID NO: 4] was amplified from pBOND3 (origin and cloning described in Example 10) using the cloning primers oBOND20 and oBOND21 (Table 11). The resulting PCR fragment was digested with restriction enzymes HindIII and NdeI, gel purified, and then ligated with T4 DNA ligase into the integrating vector pKLAC2 (New England Biolabs). The vector was then linearized with the same restriction enzymes and dephosphorylated by Quick CIP (New England Biolabs). This generated the expression vector designated as (“pBOND22”).

The pBOND22 expression vector was transformed into 10-beta competent cells (New England Biolabs) by heat-shock transformation and the transformants were selected on LB agar plates containing 100 μg/mL carbenicillin. A few colonies were cultured in LB with 100 μg/mL carbenicillin and the expression vector was purified using the Zyppy plasmid miniprep kit (Zymo Research) by following the manufacturer's instructions. The gene insert was verified by restriction digestion.

The pBOND22 expression vector was linearized using the restriction enzyme SacII and desalted using the PCR and Cleanup kit (Monarch) and transformed into an K. lactis host cell GG799 (New England Biolabs) designated as (“sBOND68”) by chemical transformation. Two negative controls were prepared, an empty vector designated as (“pBOND27”), and a control gene expressing a maltose-binding protein designated as (“pBOND28”) transformed into the same host cell. Cells were grown on YCB agar plates containing 5 mM acetamide, for 4 days at 30° C. Several colonies were patched on new YCB agar plates containing 5 mM acetamide.

Cell Culture and Protein Analysis

A few colonies were inoculated into YPGal medium (10 g/L yeast extract, 20 g/L peptone, and 20 g/l galactose) and allowed to grow at 30° C.

Samples were taken and analyzed after approximately 24 hours, when the OD600 range was about 25-30. Cell cultures were collected by centrifugation and cell pellets were weighed. The cells were lysed as described in Example 10. Equal amounts of total protein estimated by OD600 values loaded on each lane and analyzed by SDS-PAGE. Proteins were visualized by Coomassie staining, see FIG. 17 .

Results

FIG. 17 shows the SDS-PAGE gel. The molecular size of profilin is 15 kDa. Lane 1 shows the host cell (sBOND68) with pBOND22 expressing the profilin protein (clone 2). Lane 2 shows the host cell (sBOND68) with pBOND22 expressing the profilin protein (clone 3). Lane 3 shows the host cell (sBOND68) with pBOND27 empty vector. Lane 4 shows host cell (sBOND68) with pBOND28 expressing the control gene, maltose-binding protein. Lane 5 shows the molecular weight marker.

We observed a 15 kDa protein in the profilin expressing clones (lanes 1-2), while no such band was observed in the empty vector control or the control gene (lanes 3-4). These results demonstrate that a K. lactis host cell could produce a chicken profilin protein.

6.9.20. Example 20: Production of Recombinant Profilin Protein from Chicken in a Schizosaccharomyces pombe Host Cell

This study was conducted to determine if a Schizosaccharomyces pombe host cell could express a profilin protein from chicken.

Identification and Cloning of Profilin Gene from Chicken

The identification and cloning of the profilin gene were carried out as described in Example 10.

The profilin gene [SEQ ID NO: 4] was amplified from pBOND3 expression vector using primers oBOND5 and oBOND6 (see Table 11). The PCR fragment was digested with restriction enzymes XhoI and SmaI, gel purified, and then ligated into pBOND10 (REP4X [ATCC 87604]) vector which was cut with the same restriction enzymes. This placed the profilin gene in front of the S. pombe nmtl promoter and generated the expression vector designated as (“pBOND29”), which is a high copy plasmid.

The pBOND29 expression vector was introduced into an S. pombe strain, designated as (“sBOND3”) using the Zymo Research™ Frozen-EZ Yeast Transformation II kit following the manufacturer's instructions. An empty vector “pBOND10” strain was also generated using the same host cell.

Cell Culture

Cell were grown in flasks in glucose selective media, lacking uracil and containing thiamine, to repress the expression of profilin, until the culture reached an OD600 of 1. Next, cells were transferred to media lacking thiamine, to induce expression of profilin. Subsequently, the cells were grown at 37° C. for an additionally 30 hours with vigorous shaking. After, cells were collected by centrifugation.

Protein Analysis

Protein extracts were prepared by treating cells with 0.3N NaOH for 15 minutes, and then boiling the cell pellet in SDS sample buffer for 5 minutes (Matsuo, Asakawa, Toda, & Katayama, 2006, A Rapid Method for Protein Extraction from Fission Yeast. Bioscience, Biotechnology, and Biochemistry, 70(8), 1992-1994). Equal amounts of total protein were loaded on each lane and analyzed by SDS-PAGE. Proteins were visualized by Coomassie staining, see FIG. 18 .

Results

FIG. 18 shows the results of the SDS-PAGE gel. Lane 1 shows the molecular weight marker. Lane 2 shows the host cell (sBOND3) with the vector pBOND10 empty vector. Lane 3 shows the host cell (sBOND3) with pBOND29 expressing the profilin protein (clone 2). Lane 4 shows the host cell (sBOND3) with pBOND29 expressing the profilin protein (clone 3). Lane 5 shows the host cell (sBOND3) with pBOND29 expressing the profilin protein (clone 5).

We observed a protein at 15 kDa, in the sBOND3 profilin expressing clones (lanes 3-5), while no such band was observed in the pBOND10 empty vector strain (lane 2). These results demonstrate that a S. pombe host cell could express a chicken profilin protein.

6.9.21. List of Strains, Expression Vectors, and Oligonucleotides

TABLE 9 Host Cell Strains Other Name Species designation Genotype sBOND1 Saccharomyces ATCC 208288 MATalpha ura3-52 trp1 leu2- cerevisiae delta1 his 3-delta200 pep4::HIS3 prb1-delta1.6R can1 GAL sBOND2 Komagataella Pichia pastoris pep4Δ prb1Δ phaffii PPS-9016 sBOND3 Schizosaccharomyces FY118/ATCC h90 ura4-D18 pombe 201401 leu1-32 ade6-M216 sBOND28 Saccharomyces ATCC MYA-1108 MATa trp1-289 leu2-3 leu2-112 cerevisiae CEN.PK2-1Ca ura3-52 his3-delta1MATa trp1-289 leu2-3 leu2-112 ura3-52 his3-delta1 sBOND68 Kluyveromyces GG799 MATα lactis [pGKI1⁺] *MBP = Maltose binding protein

TABLE 10 Expression Vectors Bacterial Yeast Name Other designation Gene marker marker Promoter pBOND2 pD1205-Chck_Coronin Chicken CmR TRP1 GAL1 coronin pBOND3 pD1205-Chck_Profilin Chicken CmR TRP1 GAL1 profilin pBOND4 pD1248-Chck_Cofilin-2 Chicken AmpR URA3 GAL1 cofilin-2 pBOND8 pRS424 Empty AmpR TRP1 GAL1 Control pBOND10 pREP4x Empty AmpR ura4⁺ NMT1 Control pBOND11 pD1211_Myozenin-1 Turkey KanR LEU2 TEF1 myozenin1 pBOND19 pD1205_Pig_TroponinC Pig CmR TRP1 GAL1 troponinC pBOND21 pRS424 P-GAP_Cofilin2 Chicken AmpR TRP1 GAL1 cofilin-2 pBOND22 pKLAC2_P-LAC4_Profilin Chicken AmpR amdS LAC4 profilin pBOND24 pD902_Chck_Cofilin-2 Chicken ZeocinR ZeocinR AOX1 cofilin-2 pBOND25 pD902_Chck_Profilin Chicken ZeocinR ZeocinR AOX1 profilin pBOND27 pKLAC2 Empty AmpR amdS LAC4 Control pBOND28 pKLAC1-malE MBP* AmpR amdS LAC4 pBOND29 pREP4x-Chck_Profilin-5 Chicken AmpR ura4⁺ NMT1 profilin

TABLE 11 Oligonucleotide primers for cloning SEQ ID NO: Name Sequence of Oligonucleotide SEQ ID NO: 13 oBOND5 5′-ATAACTCGAGATGGCTGGCTGGCAATCTTATG-3′ SEQ ID NO: 14 oBOND6 5′-TCCCGGGTTAAAAACCGGAATCTCTCAAG-3′ SEQ ID NO: 15 oBOND11 5′-TATTCTCGAGACGGATTAGAAGCCGCCGAGC-3′ SEQ ID NO: 16 oBOND12 5′-TACAGAATTCTTTTGCGTGCAGGTGAGG-3′ SEQ ID NO: 17 oBOND20 5′-TATAAGCTTATGGCTGGCTGGCAATCTTATG SEQ ID NO: 18 oBOND21 5′-ATACCATATGTTAAAAACCGGAATCTCTCAAG

7. SEQUENCES

TABLE 1 Sequences of Animal Proteins Skeletal muscle tissue Accession SEQ ID number Common NO: (database) name/animal Amino acid sequence SEQ ID A4IFM8 Actin, alpha 1, MCDEDETTALVCDNGSGLVKAGFA NO: 19 (UniProtKB) skeletal GDDAPRAVFPSIVGRPRHQGVMVG muscle/Bos MGQKDSYVGDEAQSKKGILTLKYPI taurus EHGIITNWDDMEKIWHHTFYNELRV APEEHPTLLTEAPLNPKANREKMTQI MFETFNVPAMYVAIQAVLSLYASGR TTGIVLDSGDGVTHNVPIYEGYALPH AIMRLDLAGRDLTDYLMKILTERGY SFVTTAEREIVRDIKEKLCYVALDFE NEMATAASSSSLEKSYELPDGQVITI GNERFRCPETLFQPSFIGMESAGIHET TYNSIMKCDIDIRKDLYANNVMSGG TTMYPGIADRMQKEITALAPSTMKIK IIAPPERKYSVWIGGSILASLSTFQQM WITKQEYDEAGPSIVHRKCF SEQ ID P68137 Actin, alpha MCDEDETTALVCDNGSGLVKAGFA NO: 20 (UniProtKB) skeletal GDDAPRAVFPSIVGRPRHQGVMVG muscle/Sus MGQKDSYVGDEAQSKRGILTLKYPI scrofa EHGIITNWDDMEKIWHHTFYNELRV APEEHPTLLTEAPLNPKANREKMTQI MFETFNVPAMYVAIQAVLSLYASGR TTGIVLDSGDGVTHNVPIYEGYALPH AIMRLDLAGRDLTDYLMKILTERGY SFVTTAEREIVRDIKEKLCYVALDFE NEMATAASSSSLEKSYELPDGQVITI GNERFRCPETLFQPSFIGMESAGIHET TYNSIMKCDIDIRKDLYANNVMSGG TTMYPGIADRMQKEITALAPSTMKIK IIAPPERKYSVWIGGSILASLSTFQQM WITKQEYDEAGPSIVHRKCF SEQ ID P68139 Actin, alpha MCDEDETTALVCDNGSGLVKAGFA NO: 21 (UniProtKB) skeletal GDDAPRAVFPSIVGRPRHQGVMVG muscle/Gallus MGQKDSYVGDEAQSKRGILTLKYPI gallus EHGIITNWDDMEKIWHHTFYNELRV APEEHPTLLTEAPLNPKANREKMTQI MFETFNVPAMYVAIQAVLSLYASGR TTGIVLDSGDGVTHNVPIYEGYALPH AIMRLDLAGRDLTDYLMKILTERGY SFVTTAEREIVRDIKEKLCYVALDFE NEMATAASSSSLEKSYELPDGQVITI GNERFRCPETLFQPSFIGMESAGIHET TYNSIMKCDIDIRKDLYANNVMSGG TTMYPGIADRMQKEITALAPSTMKIK IIAPPERKYSVWIGGSILASLSTFQQM WITKQEYDEAGPSIVHRKCF SEQ ID P68138 Actin, alpha MCDEDETTALVCDNGSGLVKAGFA NO: 22 (UniProtKB) skeletal GDDAPRAVFPSIVGRPRHQGVMVG muscle/Bos MGQKDSYVGDEAQSKRGILTLKYPI taurus EHGIITNWDDMEKIWHHTFYNELRV APEEHPTLLTEAPLNPKANREKMTQI MFETFNVPAMYVAIQAVLSLYASGR TTGIVLDSGDGVTHNVPIYEGYALPH AIMRLDLAGRDLTDYLMKILTERGY SFVTTAEREIVRDIKEKLCYVALDFE NEMATAASSSSLEKSYELPDGQVITI GNERFRCPETLFQPSFIGMESAGIHET TYNSIMKCDIDIRKDLYANNVMSGG TTMYPGIADRMQKEITALAPSTMKIK IIAPPERKYSVWIGGSILASLSTFQQM WITKQEYDEAGPSIVHRKCF SEQ ID P02609 Myosin MAPKKAKRRAAEGSSNVFSMFDQT NO: 23 (UniProtKB) regulatory light QIQEFKEAFTVIDQNRDGIIDKDDLRE chain 2, skeletal TFAAMGRLNVKNEELDAMIKEASGP muscle INFTVFLTMFGEKLKGADPEDVIMG isoform/Gallu AFKVLDPDGKGSIKKSFLEELLTTQC gallus DRFTPEEIKNMWAAFPPDVAGNVDY KNICYVITHGEDKEGE SEQ ID Q9PTY2 Skeletal myosin MSSDAEMAIFGEAAPYLRKSEKERIE NO: 24 (UniProtKB) heavy AQNKPFDAKTSVFVVHAKESYVKST chain/Gallus IQSKESGKVTVKTESGETLTVKEDQI gallus FSMNPPKYDKIEDMAMMTHLHEPA VLYNLKERYAAWMIYTYSGLFCVTV NPYKWLPVYNPEVVLAYRGKKRQE APPHIFSISDNAYQFMLTDRENQSILI TGESGAGKTVNTKRVIQYFATIAASG DKKKEEQPAGKMQGTLEDQIISANP LLEAFGNAKTVRNDNSSRFGKFIRIH FGATGKLASADIETYLLEKSRVTFQL KAERSYHIFYQIMSNKKPELIEMLLIT TNPYDYHYVSQGEITVPSINDQEELM ATDSAIDILGFTPDEKTAIYKLTGAV MHYGNLKFKQKQREEQAEPDGTEV ADKAAYLMGLNSADLLKALCYPRV KVGNEFVTKGQTVQQVYNSVGALA KAVFEKMFLWMVVRINQQLDTKQP RQYFIGVLDIAGFEIFDFNSLEQLCIN FTNEKLQQFFNHHMFVLEQEEYKKE GIEWEFIDFGMDLAACIELIEKPMGIF SILEEECMFPKATDTSFKNKLYDQHL GKSNNFQKPKPGKGKAEAHFSLVHY AGTVDYNISGWLDKNKDPLYETVV GLYQKSSLKTLALLFASAGGEAESG GGGKKGGKKKGSSFQTVSALFRENL NKLMTNLRSTHPHFVRCIIPNETKTP GAMEHELVLHQLRCNGVLEGIRICR KGFPSRILYADFKQRYKVLNASAIPE GQFIDSKKASEKLLGSIDVDHTQYKF GHTKVFFKAGLLGLLEEMRDEKLAQ LITRTQARCRGFLMRVEYRRMVERR ESIFCIQYNIRSFMNVKHWPWMKLFF KIKPLLKSAESEKEMANMKGEFEKT KEELAKSGAKRKDLEGKMVSLLQEK NDLQLQVQAEADALADAEERCDQLI KTKIQLEAKIKEVTERAEDEEEINAEL TAKKRKLEDECSELKKDIDDLELTLA KVEKEKHATENKVKNLTEEMAALD ENIAKLTKEKKAPQEAHQQTLDDLQ AEEDKVNTLTKAKTKLEQQVDDLEG SLEQEKKLRMDLERAKRKLEGDLKL AHDSIMDLENDKQQLDEKLKKKDFE ISQIQSKIEDEQALGMQLQKKIKELQ ARTEELEEEIEAERTSRAKAEKHRAD LSRELEEISERLEEAGGATAAQIDMN KKREAEFQKMRRDLEEATLQHEATA AALRKKHADSTAELGEQIDNLQRVK QKLEKEKSELKMEIDDLASNMESVS KAKASLEKTCRALEDQMSEIKTKEE EHQRMINDVNAQRARLQTESGEYSR QVEEKDALISQLSRGKQAFTQQIEEL KRHLEEEIKAKNALAHGLQSARHDC DLLREQYEEEQEAKGELQRALSKAN SEVAQWRTKYETDAIQRTEELEEAK KKLAQRLQDAEEHVEAVNSKCASLE KTKQRLQNEVEDLMIDVERANSACA ALDKKQKNFDKILSEWKQKYEETQA ELEASQKESRSLSTELFKMKNAYEES LDHLETLKRENKNLQQEISDLTEQIA EGGKAIHELEKVKKQIEQEKSELQAS LEEAEASLEHEEGKILRLQLELNQVK SEIDRKIAEKDEEIDQLKRNHLRIVES MQRTLDAEVRSRNEALRLKKKMEG DLNEMEIQLNHANRMAAEAQKNLR NTQGVLKDTQIHLDDALRSQEDLKE QVAMVERRANLLQAETEELRAALEQ TERSRKVAEQELLDASERVQLLHTQ NTSLINTKKKLESDISQIQSEMEDTIQ EARNAEEKAKKAITDAAMMAEELK KEQDTSAHLERMKKNLDQTVKDLQ HRLDEAEQLALKGGKKQIQKLEARV RELEGEVDAEQKRSAEAVKGVRKYE RRVKELTYQSEEDRKNVLRLQDLVD KLQMKVKSYKRQAEEAEELSNVNLS KFRKIQHELEEAEERADIAESQVNKL RVKSREFHKKIEEEEI SEQ ID A0A1S3L3X1 Myosin heavy MSTDAEMQVYGKAAIYLRKSEKER NO: 25 (UniProtKB) chain, fast MEAQAMPFDSKNSCYVTDKVELYL skeletal muscle- KGLVTARADGKCTVTVTKPDGTKEE like GKEFKDADIYEMNPPKYDKIEDMAM isoform/Salmo MTYLNEASVLYNLKERYAAWMIYT salar YSGLFCATVNPYKWLPVYDEEVVN AYRGKKRVEAPPHIFSVSDNAFQFM MIDKENQSVLITGESGAGKTVNTKR VIQYFATIAVSGGKKEADPNKMQGS LEDQIIAANPLLESYGNAKTVRNDNS SRFGKFIRIHFQAGKLAKADIETYLLE KSRVSFQLPDERGYHIFFQMMTGHK PELVELALLTTNPYDFPMCSQGQIAV ASINDNEELDATDEAITILGFTNEEKL GIYKLTGAVVHHGNLKFKQKQREEQ AEPDGTEVADKIAYLLGLNSAEMLK ALCYPRVKVGNEYVTKGQTVAQVN NSVSALAKSIYERMFLWMVIRINEM LDTKNPRQFYIGVLDIAGFEIFDYNS MEQLCINFTNEKLQQFFNHTMFVLE QEEYKKEGIVWAFIDFGMDLAACIEL IEKPLGIFSILEEECMFPKSSDTTFKDK LYAQHLGKTKAFEKPKPAKGKAEA HFSLVHYAGTVDYNITGWLEKNKDP LNDSVCQLYGKSGVKILAALYPPPPP EDKAKKGGKKKGGSMQTVSSQFRE NLHKLMTNLRSTHPHFVRCLIPNESK TPGLMENFLVIHQLRCNGVLEGIRIC RKGFPSRIIYADFKQRYKVLNASVIPE GQFMDNKKASEKLLGSIDVNHEDYK FGHTKVFFKAGLLGVLEEMRDEKLA TLVGMVQALSRGFLMRREFSKMME RRESIYAIQYNIRSFMNVKTWPWMK LYFKIKPLLQSAETEKELANMKENYE KMKTDLAKALSTKKQMEEKLVSLT QEKNDLALQVASEGESLNDAEERCE GLIKSKIQQEAKLKETTERLEDEEEIN AELTAKKRKLEDECSELKKDIDDLEL TLAKVEKEKHATENKVKNLTEEMAS MDESVAKLTKEKKALQEAHQQTLD DLQAEEDKVNTLTKAKTKLEQQVD DLEGSLEQEKKLRMDLERAKRKLEG DLKLAQESIMDLENDKQQADEKIKK KEFETTQLLSKIEDEQSLGAQLQKKI KELQARIEELEEEIEAERAARAKVEK QRADLSRELEEISERLEEAGGATAAQ IEMNKKREAEFQKLRRDLEESTLQHE ATAAALRKKQADSVAELGEQIDNLQ RVKQKLEKEKSEYKMEIDDLSSNME AVAKAKGNLEKMCRTLEDQLSELKT KNDENVRQVNDISGQRARLLTENGE FGRQLEEKEALVSQLTRGKQAFTQQ VEELKRLIEEEVKAKNALAHGVQSA RHDCDLLREQFEEEQEAKAELQRGM SKANSEVAQWRTKYETDAIQRTEEL EEAKKKLAQRLQEAEETIEATNSKC ASLEKTKQRLQGEVEDLMIDVERAN ALAANLDKKQRNFDKVLAEWKQKY EEGQAELEGAQKEARSMSTELFKMK NSYEEALDHLETLKRENKNLQQEISD LTEQIGETGKSIHELEKAKKTVETEK SEIQTALEEAEGTLEHEESKILRVQLE LNQIKGEVDRKIAEKDEEMEQIKRNS QRVVDSMQSTLDSEVRSRNDALRVK KKMEGDLNEMEIQLSHSNRQAAEAQ KQLRNVQGQLKDAQLHLDDAVRAA EDMKEQAAMVERRNGLMVAEIEEL RVALEQTERGRKVAETELVDASERV GLLHSQNTSLLNTKKKLETDLVQVQ GEVDDIVQEARNAEEKAKKAITDAA MMAEELKKEQDTSSHLERMKKNLE VTVKDLQHRLDEAENLAMKGGKKQ LQKLESRVRELETEVEAEQRRGVDA VKGVRKYERRVKELTYQTEEDKKN VNRLQDLVDKLQMKVKAYKRQAEE AEEAANQHMSKFRKVQHELEEAEER ADIAETQVNKLRAKTRDSGKGKEAA E SEQ ID A0A1S3QIZ8 Myosin heavy MSTDAEMQIYGKAAIYLRKSEKERM NO: 26 (UniProtKB) chain, fast EAQAAPFDSKNSCYVADKVELYLKG skeletal muscle- LITARADGKCTVTVTKPDGTKEEGK like/Salmo salar EFKDADIYEMNPPKYDKIEDMAMM TYLNEASVLYNLKERYAAWMIYTYS GLFCATVNPYKWLPVYDAEVVNAY RGKKRMEAPPHIFSVSDNAFQFMLID KENQSVLITGESGAGKTVNTKRVIQY FATIAVSGGEKKKEVDPSKMQGSLE DQIIAANPLLEAYGNAKTVRNDNSSR FGKFIRIHFQGGKLAKADIETYLLEKS RVSFQLPDERGYHIFFQMMTGHKPEI VEMALITTNPYDFPMCSQGQIAVASI DDKEELDATDDAITILGFTNDEKIGIY KLTGAVVHHGNLKFKQKQREEQAE PDGTEVADKIGYLLGLNSAEMLKAL CYPRVKVGNEYVTKGQTVPQVNNS VMALAKSIYERMFLWMVIRINEMLD TKNPRQFYIGVLDIAGFEIFDYNSME QLCINFTNEKLQQFFNHTMFVLEQEE YKKEGIVWAFIDFGMDLAACIELIEK PLGIFSILEEECMFPKSSDTTFKDKLY SQHLGKTQAFEKPKPAKGKAEAHFS LVHYAGTVDYNITGWLEKNKDPLN DSVCQLYGKSGVKILAALYPAAPPE DTTKKGGKKKGGSMQTVSSQFRENL HKLMTNLRSTHPHFVRCLIPNESKTP GLMENFLVIHQLRCNGVLEGIRICRK GFPSRIIYADFKQRYKVLNASVIPEG QFMDNKKASEKLLGSIDVNHEDYKF GHTKVSQILYFKIKPLLQSAETEKEL ANMKENYEKMTADLAKALSTKKQ MEEKLVALMQEKNDLALQVAS A0JNJ5 Myosin light MAPKKDVKKPAAAAAPAPAPAPAP (UniProtKB) chain 1/3, APAPAPPKEEKIDLSAIKIEFSKQQQD skeletal muscle EFKEAFLLFDRTGECKITLSQVGDVL isoform/Bos RALGTNPTNAEVKKVLGNPSNEEMN taurus AKKIEFEQFLPMLQAISNNKDQGTYE DFVEGLRVFDKEGNGTVMGAELRH VLATLGEKMKEEEVEALMAGQEDS NGCINYEAFVKHIMSN SEQ ID P02604 Myosin light MAPKKDVKKPAAAAAPAPAPAPAP NO: 27 (UniProtKB) chain 1, skeletal APAPAKPKEPAIDLKSIKIEFSKEQQD muscle DFKEAFLLFDRTGDAKITLSQVGDIV isoform/Gallus RALGQNPTNAEINKILGNPSKEEMNA gallus KKITFEEFLPMLQAAANNKDQGTFE DFVEGLRVFDKEGNGTVMGAELRH VLATLGEKMTEEEVEELMKGQEDSN GCINYEAFVKHIMSV SEQ ID P02605 Myosin light MSFSPDEINDFKEAFLLFDRTGDAKI NO: 28 (UniProtKB) chain 3, skeletal TLSQVGDIVRALGQNPTNAEINKILG muscle/Gallus NPSKEEMNAKKITFEEFLPMLQAAA gallus NNKDQGTFEDFVEGLRVFDKEGNGT VMGAELRHVLATLGEKMTEEEVEEL MKGQEDSNGCINYEAFVKHIMSV SEQ ID B5DGT2 Myosin light MADAAPAEASGASAFTADQIEDFKE NO: 29 (UniProtKB) chain 3, skeletal AFGLFDRVGDSMIGYNQVADVMRA muscle/Salmo LGQNPQNKEVAAILGKPSADDMAN salar KRLAFADFMPMMEKVDKIVKGTLD DYVEGLRVFDKEGNGTVSGAELRIV LGTLGEKMSEAEIDSLLIGQEDENGSI NYEAFVKHVLSV SEQ ID P13538 Myosin heavy MASPDAEMAAFGEAAPYLRKSEKER NO: 30 (UniProtKB) chain, skeletal IEAQNKPFDAKSSVFVVHPKESFVKG muscle, adult/ TIQSKEGGKVTVKTEGGETLTVKED Gallus gallus QVFSMNPPKYDKIEDMAMMTHLHE PAVLYNLKERYAAWMIYTYSGLFCV TVNPYKWLPVYNPEVVLAYRGKKR QEAPPHIFSISDNAYQFMLTDRENQSI LITGESGAGKTVNTKRVIQYFATIAA SGEKKKEEQSGKMQGTLEDQIISANP LLEAFGNAKTVRNDNSSRFGKFIRIH FGATGKLASADIETYLLEKSRVTFQL PAERSYHIFYQIMSNKKPELIDMLLIT TNPYDYHYVSQGEITVPSIDDQEELM ATDSAIDILGFSADEKTAIYKLTGAV MHYGNLKFKQKQREEQAEPDGTEV ADKAAYLMGLNSAELLKALCYPRV KVGNEFVTKGQTVSQVHNSVGALA KAVYEKMFLWMVIRINQQLDTKQPR QYFIGVLDIAGFEIFDFNSFEQLCINFT NEKLQQFFNHHMFVLEQEEYKKEGI EWEFIDFGMDLAACIELIEKPMGIFSI LEEECMFPKATDTSFKNKLYDQHLG KSNNFQKPKPAKGKAEAHFSLVHYA GTVDYNISGWLEKNKDPLNETVIGL YQKSSVKTLALLFATYGGEAEGGGG KKGGKKKGSSFQTVSALFRENLNKL MANLRSTHPHFVRCIIPNETKTPGAM EHELVLHQLRCNGVLEGIRICRKGFP SRVLYADFKQRYRVLNASAIPEGQF MDSKKASEKLLGSIDVDHTQYRFGH TKVFFKAGLLGLLEEMRDDKLAEIIT RTQARCRGFLMRVEYRRMVERRESI FCIQYNVRSFMNVKHWPWMKLFFKI KPLLKSAESEKEMANMKEEFEKTKE ELAKSEAKRKELEEKMVVLLQEKND LQLQVQAEADSLADAEERCDQLIKT KIQLEAKIKEVTERAEDEEEINAELTA KKRKLEDECSELKKDIDDLELTLAK VEKEKHATENKVKNLTEEMAVLDE TIAKLTKEKKALQEAHQQTLDDLQV EEDKVNTLTKAKTKLEQQVDDLEGS LEQEKKLRMDLERAKRKLEGDLKLA HDSIMDLENDKQQLDEKLKKKDFEI SQIQSKIEDEQALGMQLQKKIKELQA RIEELEEEIEAERTSRAKAEKHRADLS RELEEISERLEEAGGATAAQIEMNKK REAEFQKMRRDLEEATLQHEATAAA LRKKHADSTAELGEQIDNLQRVKQK LEKEKSELKMEIDDLASNMESVSKA KANLEKMCRTLEDQLSEIKTKEEQN QRMINDLNTQRARLQTETGEYSRQA EEKDALISQLSRGKQGFTQQIEELKR HLEEEIKAKNALAHALQSARHDCEL LREQYEEEQEAKGELQRALSKANSE VAQWRTKYETDAIQRTEELEEAKKK LAQRLQDAEEHVEAVNAKCASLEKT KQRLQNEVEDLMVDVERSNAACAA LDKKQKNFDKILAEWKQKYEETQTE LEASQKESRSLSTELFKMKNAYEESL DHLETLKRENKNLQQEIADLTEQIAE GGKAVHELEKVKKHVEQEKSELQAS LEEAEASLEHEEGKILRLQLELNQIKS EIDRKIAEKDEEIDQLKRNHLRIVES MQSTLDAEIRSRNEALRLKKKMEGD LNEMEIQLSHANRMAAEAQKNLRNT QGTLKDTQIHLDDALRTQEDLKEQV AMVERRANLLQAEVEELRGALEQTE RSRKVAEQELLDATERVQLLHTQNT SLINTKKKLETDIVQIQSEMEDTIQEA RNAEEKAKKAITDAAMMAEELKKE QDTSAHLERMKKNMDQTVKDLHVR LDEAEQLALKGGKKQLQKLEARVRE LEGEVDSEQKRSAEAVKGVRKYERR VKELTYQCEEDRKNILRLQDLVDKL QMKVKSYKRQAEEAEELSNVNLSKF RKIQHELEEAEERADIAESQVNKLRV KSREIHGKKIEEEE SEQ ID Q8AXY6 Muscle, skeletal MRDLLVVPLGHVLTLAALSLAETLQ NO: 31 (UniProtKB) receptor tyrosine KAPFISTPLETVDALVEDVPKFVCVV protein ESYPEPEITWTRNSIPIRLFDTRYSIQR kinase/Gallus NGQLLTILSVEDSDDGVYCCTADNG gallus VGAAAQSCGALQVKMRPKITRPPVN VEIIEGLKAVLPCTTMGNPKPSVSWI KGETVVKENARIAVLDSGNLRIHNV QREDAGQYRCVAKNSLGSAYSKPAT VVVEVFARILKAPESQNITFGSMVTL RCTAAGAPVPTVTWLENGKAVSAGS IAESVKDRVVDSRLQVYVTRPGLFTC LATNKHSKTFGAAKAAATISVSEWS KLYKGDAGYCSTYRGEVCSAILSRN ALVFFNSSYADPEETQELLVHTAWT ELKTVSSFCQPAAESLLCNYIFQECK PSGVGPAPKPICRENCLAVKDLYCFK EWLSMEENSQRGIYKPGLMLLALPE CNRLPSLHQDPSACTHIPFFDFKKENI TRTCYSGNGQFYQGWANVTASGIPC QKWSDQAPHLHRRTPQVFPELSDAE NYCRNPGGENERPWCYTKDPSVTW EYCSVSPCGDASLSLGTRKPNGETQN LPPPPSYSPTYSMNVIILIISSFALIVIL GIITLVCCRRRKQWKNKKRESETPTL TTLPSELLLDRLHPNPMYQRMPLLLN PKLLSLEYPRNNIEYVRDIGEGAFGR VFQARAPGLLPYEPFTMVAVKMLKE EASADMQADFQREAALMAEFDNPNI VKLLGVCAVGKPMCLLFEYMAYGD LNEYLRDRSPRNLCSLVQGGLEARA CLLNPLALCCTSQLCIAKQVAAGMA YLSERKFVHRDLATRNCLVGENMV VKIADFGLSRNMYSADYYKANEND AIPIRWMPPESIFYNRYTTESDVWAY GVVLWEIFSYGMQPYYGMAHEEVIY YVRDGNILSCPDNCPLELYNLMRLC WSKLPADRPSFASIHRILERMYERAV ASPQV SEQ ID P02588 Troponin C, MASMTDQQAEARAFLSEEMIAEFKA NO: 32 (UniProtKB) skeletal AFDMFDADGGGDISTKELGTVMRM muscle/Gallus LGQNPTKEELDAIIEEVDEDGSGTIDF gallus EEFLVMMVRQMKEDAKGKSEEELA NCFRIFDKNADGFIDIEELGEILRATG EHVTEEDIEDLMKDSDKNNDGRIDF DEFLKMMEGVQ SEQ ID P02587 Troponin C, TDQQAEARSYLSEEMIAEFKAAFDM NO: 33 (UniProtKB) skeletal FDADGGGDISVKELGTVMRMLGQTP muscle/Sus TKEELDAIIEEVDEDGSGTIDFEEFLV scrofa MMVRQMKEDAKGKSEEELAECFRIF DRNMDGYIDAEELAEIFRASGEHVT DEEIESIMKDGDKNNDGRIDFDEFLK MMEGVQ SEQ ID P68246 Troponin 1, fast MSDEEKKRRAATARRQHLKSAMLQ NO: 34 (UniProtKB) skeletal LAVTEIEKEAAAKEVEKQNYLAEHC muscle/Gallus PPLSLPGSMQELQELCKKLHAKIDSV gallus DEERYDTEVKLQKTNKELEDLSQKL FDLRGKFKRPPLRRVRMSADAMLRA LLGSKHKVNMDLRANLKQVKKEDT EKEKDLRDVGDWRKNIEEKSGMEG RKKMFEAGES SEQ ID P68247 Troponin 1, fast MSDEEKKRRAATARRQHLKSAMLQ NO: 35 (UniProtKB) skeletal LAVTEIEKEAAAKEVEKQNYLAEHC muscle/Cotumix PPLSLPGSMQE japonica LQELCKKLHAKIDSVDEERYDTEVK LQKTNKELEDLSQKLFDLRGKFKRPP LRRVRMSAD AMLRALLGSKHKVNMDLRANLKQV KKEDTEKEKDLRDVGDWRKNIEEKS GMEGRKKMFEA GES SEQ ID Q8MKI3 Troponin T, fast MSDEEVEHVEEEYEEEEEAQEEAPPP NO: 36 (UniProtKB) skeletal PAEVPEVHEEVHEVHEPEEVQEEEKP muscle/Bos RPRLTAPKIPEGEKVDFDDIQKKRQN taurus KDLMELQALIDSHFEARKKEEEELV ALKERIEKRRAERAEQQRIRAEKERE RQNRLAEEKARREEEDAKRRAEDDL KKKKALSSMGANYSSYLAKADQKR GKKQTAREMKKKVLAERRKPLNIDH LSEDKLRDKAKELWDTLYQLETDKF EYGEKLKRQKYDITNLRSRIDQAQK HSKKAGTAPKGKVGGRWK SEQ ID Q75ZZ6 Troponin T, MSDAEEQEYEEEQPEEEEAAEEEEAP NO: 37 (UniProtKB) slow skeletal EEPEPVAEREEERPKPSRPVVPPLIPP muscle/Sus KIPEGERVDFDDIHRKRMEKDLLELQ scrofa TLIDVHFEQRKKEEEELVALKERIER RRAERAEQQRFRTEKERERQAKLAE EKMRKEEEEAKKRAEDDAKKKKVL SNMGAHFGGYLVKAEQKRGKRQTG REMKQRILSERKKPLNIDHMGEDQL REKAQELSDWIHQLESEKFDLMAKL KQQKYEINVLYNRISHAQKFRKGAG KGRVGGRWK SEQ ID NP_990105.1 Tropomodulin- MTSYRQELEKYRDIDEDKILQELSAE NO: 38 (NCBI) 4/Gallus gallus ELEQLDTELLEMDPENVLLPAGLRQ RDQTQKSPTGPLDREALLQHLEKQA LEAKEREDLVPFTGEKKGKPFVPKNP TREIPREEQITLEPELEEALANATEAE MCDIAAILGMYTLMSNKQYYDAICS GTITNTEGINSVVKPDKYKPVPDEPP NPTNVEETLRQIQANDSALEDVNLN NIKDIPISTLKAICEAMKTNTHVKKLS LVATRSNDPVATAVAEMLAENKTLQ SLNIESNFITSAGMMSVIKAMYQNST LSELKVDNQCQRLGNTVEMEMATM LEQCPSVVRFGYHFTQQGPRARAAI AITRNNELRRKQKKT SEQ ID P68139 Alpha-actin- MCDEDETTALVCDNGSGLVKAGFA NO: 39 (UniProtKB) 1/Gallus gallus GDDAPRAVFPSIVGRPRHQGVMVG MGQKDSYVGDEAQSKRGILTLKYPI EHGIITNWDDMEKIWHHTFYNELRV APEEHPTLLTEAPLNPKANREKMTQI MFETFNVPAMYVAIQAVLSLYASGR TTGIVLDSGDGVTHNVPIYEGYALPH AIMRLDLAGRDLTDYLMKILTERGY SFVTTAEREIVRDIKEKLCYVALDFE NEMATAASSSSEEKSYELPDGQVITI GNERFRCPETLFQPSFIGMESAGIHET TYNSIMKCDIDIRKDLYANNVMSGG TTMYPGIADRMQKEITALAPSTMKIK IIAPPERKYSVWIGGSILASLSTFQQM WITKQEYDEAGPSIVHRKCF SEQ ID P20111 Alpha-actinin- MNSMNQIETNMQYTYNYEEDEYMT NO: 40 (UniProtKB) 2/Gallus gallus QEEEWDRDLLLDPAWEKQQRKTFT AWCNSHLRKAGTQIENIEEDFRNGL KLMLLLEVISGERLPKPDRGKMRFH KIANVNKALDYIASKGVKLVSIGAEE IVDGNVKMTLGMIWTIILRFAIQDISV EETSAKEGLLLWCQRKTAPYRNVNI QNFHLSWKDGLGLCALIHRHRPDLI DYSKLNKDDPIGNINLAMEIAEKHLD IPKMLDAEDIVNTPKPDERAIMTYVS CFYHAFAGAEQAETAANRICKVLAV NQENERLMEEYERLASELLEWIRRTI PWLENRTPEKTMQAMQKKLEDFRD YRRKHKPPKVQEKCQLEINFNTLQT KLRISNRPAFMPSEGKMVSDIAGAW QRLEQAEKGYEEWLLNEIRRLERLE HLAEKFRQKASTHEQWAYGKEQILL QKDYESASLTEVRAMLRKHEAFESD LAAHQDRVEQIAAIAQELNELDYHD AASVNDRCQKICDQWDSLGTLTQKR REALERTEKLLETIDQLHLEFAKRAA PFNNWMEGAMEDLQDMFIVHSIEEI QSLISAHDQFKATLPEADGERQAILSI QNEVEKVIQSYSMRISASNPYSTVTV EEIRTKWEKVKQLVPQRDQSLQEEL ARQHANERLRRQFAAQANVIGPWIQ TKMEEIARSSIEMTGPLEDQMNQLK QYEQNIINYKHNIDKLEGDHQLIQEA LVFDNKHTNYTMEHIRVGWELLLTT IARTINEVETQILTRDAKGITQEQMN DFRASFNHFDRRKNGLMDHDDFRA CLISMGYDLGEAEFARIMSLVDPNG QGTVTFQSFIDFMTRETADTDTAEQV IASFRILASDKPYILADELRRELPPEQ AQYCIKRMPQYTGPGSVPGALDYTS FSSALYGESDL SEQ ID P20111-2 Alpha-actinin- MNSMNQIETNMQYTYNYEEDEYMT NO: 41 (UniProtKB) 2/Gallus gallus QEEEWDRDLLLDPAWEKQQRKTFT AWCNSHLRKAGTQIENIEEDFRNGL KLMLLLEVISGERLPKPDRGKMRFH KIANVNKALDYIASKGVKLVSIGAEE IVDGNVKMTLGMIWTIILRFAIQDISV EETSAKEGLLLWCQRKTAPYRNVNI QNFHLSWKDGLGLCALIHRHRPDLI DYSKLNKDDPIGNINLAMEIAEKHLD IPKMLDAEDIVNTPKPDERAIMTYVS CFYHAFAGAEQAETAANRICKVLAV NQENERLMEEYERLASELLEWIRRTI PWLENRTPEKTMQAMQKKLEDFRD YRRKHKPPKVQEKCQLEINFNTLQT KLRISNRPAFMPSEGKMVSDIAGAW QRLEQAEKGYEEWLLNEIRRLERLE HLAEKFRQKASTHEQWAYGKEQILL QKDYESASLTEVRAMLRKHEAFESD LAAHQDRVEQIAAIAQELNELDYHD AASVNDRCQKICDQWDSLGTLTQKR REALERTEKLLETIDQLHLEFAKRAA PFNNWMEGAMEDLQDMFIVHSIEEI QSLISAHDQFKATLPEADGERQAILSI QNEVEKVIQSYSMRISASNPYSTVTV EEIRTKWEKVKQLVPQRDQSLQEEL ARQHANERLRRQFAAQANVIGPWIQ TKMEEIARSSIEMTGPLEDQMNQLK QYEQNIINYKHNIDKLEGDHQLIQEA LVFDNKHTNYTMEHIRVGWELLLTT IARTINEVETQILTRDAKGITQEQMN DFRASFNHFDRRKNGLMDHDDFRA CLISMGYDLDESDNLHSDEFKACLIS LGEVGNDLQGEAEFARIMSLVDPNG QGTVTFQSFIDFMTRETADTDTAEQV IASFRILASDKPYILADELRRELPPEQ AQYCIKRMPQYTGPGSVPGALDYTS FSSALYGESDL SEQ ID P13127 F-actin-capping MADFEDRVSDEEKVRIAAKFITHAPP NO: 42 (UniProtKB) protein subunit GEFNEVFNDVRLLLNNDNLLREGAA alpha-1/Gallus HAFAQYNMDQFTPVKIEGYDDQVLI gallus TEHGDLGNGRFLDPRNKISFKFDHLR KEASDPQPEDTESALKQWRDACDSA LRAYVKDHYPNGFCTVYGKSIDGQQ TIIACIESHQFQPKNFWNGRWRSEWK FTITPPTAQVAAVLKIQVHYYEDGNV QLVSHKDIQDSVQVSSDVQTAKEFIK IIENAENEYQTAISENYQTMSDTTFK ALRRQLPVTRTKIDWNKILSYKIGKE MQNA SEQ ID P28497 F-actin-capping MADLEEQLSDEEKVRIAAKFIIHAPP NO: 43 (UniProtKB) protein subunit GEFNEVFNDVRLLLNNDNLLREGAA alpha-2/Gallus HAFAQYNLDQFTPVKIDGYDEQVLIT gallus EHGDLGNGKFLDPKNKISFKFDHLR KEATDPRPHEVENAIESWRNSVETA MKAYVKEHYPNGVCTVYGKTIDGQ QTIIACIESHQFQAKNFWNGRWRSE WKFTISPSTTQVAGILKIQVHYYEDG NVQLVSHKDIQDSLTVSNEAQTAKE FIKIVEAAENEYQTAISENYQTMSDT TFKALRRQLPVTRTKIDWNKILSYKI GKEMQNA SEQ ID A0M8U0 Capping protein MADLEEQLSDEEKVRIAAKFIIHAPP NO: 44 (UniProtKB) (Actin filament) GEFNEVFNDVRLLLNNDNLLREGAA muscle Z-line, HAFAQYNLDQFTPVKIDGYDEQVLIT alpha 2/Gallus EHGDLGNGKFLDPKNKISFKFDHLR gallus KEATDPRPHEVENAIESWRNSVETA MKAYVKEHYPNGVCTVYGKTIDGQ QTIIACIESHQFQAKNFWNGRWRSE WKFTISPSTTQVAGILKIQVHYYEDG NVQLVSHKDIQDSLTVSNEAQTAKE FIKIVEAAENEYQTAISENYQTMSDT TFKALRRQLPVTRTKIDWNKILSYKI GKEMQNA SEQ ID NP_001265047.1 F-actin-capping MSVGQGLCESEKVSLICGLMRQSPP NO: 45 (NCBI) protein subunit GEFRQVVQDLCDLLQDDELVKQQA alpha-3/Gallus ARAGARHNKNNFTPVLVNGNTVLLT gallus QYNDLGGNRFFYPQDKFSFEFDHLS GVTSKTHLHRVMLDEGELWRGALH KGLNAYVNYHFPVGNCCVFKKSLG KRQMLVACIEAHQYQPSKHWNSLW KSDWTFSLTPVMTRVTGIFLLQLHYF RNANLHVTISKSVSESLHVIDRNQFV TDFVKFVKTEDNKIHNAILENIQALS EHTWRKNLRRRLPITRTFMNWNELL NNQHLKTGVSRKEVPP SEQ ID P02565 Myosin-1B/ MATDADMAIFGEAAPYLRKSEKERI NO: 46 (UniProtKB) Gallus gallus EAQNKPFDAKSSVFWHAKESYVKS TIQSKESGKVTVKTEGGETLTVKEDQ IFSMNPPKYDKIEDMAMMTHLHEPA VLYNLKERYAAWMIYTYSGLFCVTV NPYKWLPVYNPEVVLAYRGKKRQE APPHIFSISDNAYQFMLTDRENQSILI TGESGAGKTVNTKRVIQYFATIAASG DKKKEEQPAGKMQGTLEDQIISANP LLEAFGNAKTVRNDNSSRFGKFIRIH FGATGKLASADIETYLLEKSRVTFQL KAERSYHIFYQIMSNKKPELIEMLLIT TNPYDYQYVSQGEITVPSINDQEELM ATDSAIDILGFTPDEKTAIYKLTGAV MHYGNLKFKQKQREEQAEPGGTEV ADKAAYLMGLNSADLLKALCYPRV KVGNEYVTKGQTVQQVYNSVGALA KSVFEKMFLWMVVRINQQLDTKQP RQYFIGVLDIAGFEIFDFNSLEQLCIN FTNEKLQQFFNHHMFVLEQEEYKKE GIEWEFIDFGMDLAACIELIEKPMGIF SILEEECMFPKATDTSFKNKLYDQHL GKSNNFQKPKPGKGKAEAHFSLVHY AGTVDYNITGWLEKNKDPLNETVVG LYQKSSLKTLALLFASVGGAEAESG AGGKKGGKKKGSSFQTVSALFRENL NKLMSNLRSTHPHFVRCLIPNETKTP GAMEHELVLHQLRCNGVLEGIRICR KGFPIRILYADFKQRYKVLNASAIPE GQFIDSKKASEKLLGSIDVDHTQYKF GHTKVFFKAGLLGLLEEMRDEKLAQ LITRTQARCRGFLMRVEFKKMMERR ESIFCIQYNVRAFMNVKHWPWMKLF FKIKPLLKSAESEKEMANMKEEFEKT KEELAKSEAKRKELEEKMVSLLQEK NDLQLQVQAEADGLADAEERCDQLI KTKIQLEAKIKELTERAEDEEEMNAE LTAKKRKLEDECSELKKDIDDLELTL AKVEKEKHATENKVKNLTEEMAAL DETIAKLTKEKKALQEAHQQTLDDL QAEEDKVNTLTKAKTKLEQQVDDLE GSLEQEKKLRMDLERAKRKLEGDLK MTQESTMDLENDKQQLDEKLKKKD FEISQIQSKIEDEQALGMQLQKKIKEL QARIEELEEEIEAERTSRAKAEKHRA DLSRELEEISERLEEAGGATAAQIDM NKKREAEFQKMRRDLEEATLQHEAT AAALRKKHADSTADVGEQIDNLQRV KQKLEKEKSELKMEIDDLASNMESV SKAKANLEKMCRSLEDQLSEIKTKEE EQQRTINDISAQKARLQTESGEYSRQ VEEKDALISQLSRGKQAFTQQIEELK RHLEEEIKAKKCPAHALQSARHDCD LLREQYEEEQEAKGELQRALSKANS EVAQWRTKYETDAIQRTEELEEAKK KLAQRLQDAEEHVEAVNSKCASLEK TKQRLQNEVEDLMIDVERSNAACAA LDKKQKNFDKILSEWKQKYEETQAE LEASQKESRSLSTELFKMKNAYEESL DHLETLKRENKNLQQEISDLTEQIAE GGKAIHELEKVKKQIEQEKSELQTAL EEAEASLEHEEGKILRVQLELNQVKS DIDRKIAEKDEEIDQLKRNHLRVVDS MQSTLDAEIRSRNEALRLKKKMEGD LNEIEIQLSHANRQAAEAQKNLRNTQ GVLKDTQIHLDDALRSQEDLKEQVA MVERRANLLQAEIEELRAALEQTERS RKVAEQELLDASERVQLLHTQNTSLI NTKKKLESDISQIQSEMEDTIQEARN AEEKAKKAITDAAMMAEELKKEQD TSAHLERMKKNLDQTVKDLQHRLD EAEQLALKGGKKQIQKLEARVRELE GEVDAEQKRSAEAVKGVRKYERRV KELTYQSEEDRKNVLRLQDLVDKLQ MKVKSYKRQAEEAEELSNVNLSKFR KIQHELEEAEERADIAESQVNKLRAK SREIGKKAESEE SEQ ID Q9TV63 Myosin-2/Sus MSSDQEMAIFGEAAPYLRKSEKERIE NO: 47 (UniProtKB) scrofa AQNRPFDAKTSVFVAEPKESFVKGTI QSREGGKVTVKTEAGATLTVKEDQV FPMNPPKFDKIEDMAMMTHLHEPGV LYNLKERYAAWMIYTYSGLFCVTVN PYKWLPVYNPEVVTAYRGKKRQEA PPHIFSISDNAYQFMLTDRENQSILIT GESGAGKTVNTKRVIQYFATIAVTGE KKKEEPTSGKMQGTLEDQIISANPLL EAFGNAKTVRNDNSSRFGKFIRIHFG TTGKLASADIETYLLEKSRVTFQLKA ERSYHIFYQITSNRKPELIEMLLITTNP YDYPFISQGEISVASIDDQEELIATDS AIDILGFTNEEKVSIYKLTGAVMHYG NLKFKQKQREEQAEPDGTEVADKA AYLQSLNSADLLKALCYPRVKVGNE YVTKGQTVEQVTNAVGALAKAVYE KMFLWMVTRINQQLDTKQPRQYFIG VLDIAGFEIFDFNSLEQLCINFTNEKL QQFFNHHMFVLEQEEYKREGIEWTFI DFGMDLAACIELIEKPMGIFSILEEEC MFPKATDTSFKNKLYEQHLGKSANF QKPKPAKGKVEAHFSLIHYAGTVDY NITGWLDKNKDPLNDTVVGLYQKS ALKTLAFLFSGAQTGEAEAGGTKKG GKKKGSSFQTVSALFRENLNKLMTN LRSTHPHFVRCIIPNETKTPGAMEHE LVLHQLRCNGVLEGIRICRKGFPSRIL YADFKQRYKVLNASAIPEGQYIDSK KASEKLLASIDIDHTQYKFGHTKVFF KAGLLGLLEEMRDDKLAQLITRTQA RCRGFLARVEYQKMVERRESIFCIQY NIRAFMNVKHWPWMKLFFKIKPLLK SAESEKEMATMKEEFQKTKDELAKS EAKRKELEEKMVTLLKEKNDLQLQV QAEAEGLADAEERCDQLIKTKIQLEA KIKEVTERAEDEEEINAELTAKKRKL EDECSELKKDIDDLELTLAKVEKEKH ATENKVKNLTEEMAGLDETIAKLTK EKKALQEAHQQTLDDLQAEEDKVN TLTKAKTKLEQQVDDLEGSLEQEKK LRMDLERAKRKLEGDLKLAQESIMD IENEKQQLDEKLKKKEFEISNLQSKIE DEQALAIQLQKKIKELQARIEELEEEI EAERASRAKAEKQRSDLSRELEEISE RLEEAGGATSAQIEMNKKREAEFQK MRRDLEEATLQHEATAAALRKKHA DSVAELGEQIDNLQRVKQKLEKEKS EMKMEIDDLASNMETVSKAKGNLE KMCRTLEDQLSELKSKEEEQQRLIND LTAQRGRLQTESGEFSRQLDEKEAL VSQLSRGKQAYTQQIEELKRQLEEEI KAKNALAHALQSSRHDCDLLREQYE EEQESKAELQRALSKANTEVAQWRT KYETDAIQRTEELEEAKKKLAQRLQ AAEEHVEAVNAKCASLEKTKQRLQ NEVEDLMLDVERTNAACAALDKKQ RNFDKILAEWKQKYEETHAELEASQ KEARSLGTELFKMKNAYEESLDQLE TLKRENKNLQQEISDLTEQIAEGGKR IHELEKIKKQVEQEKSEIQAALEEAE ASLEHEEGKILRIQLELNQVKSEVDR KIAEKDEEIDQLKRNHVRVVESMQS MLDAEIRSRNDAIRLKKKMEGDLNE MEIQLNHANRMAAEALRNYRNTQGI LKDTQIHLDDALRGQEDLKEQLAMV ERRANLLQAEIEELRATLEQTERSRK VAEQELLDASERVQLLHTQNTSLINT KKKLETDISQMQGEMEDILQEARNA EEKAKKAITDAAMMAEELKKEQDTS AHLERMKKNMEQTVKDLQHRLDEA EQLALKGGKKQIQKLEARVRELEGE VESEQKRNAEAVKGLRKHERRVKEL TYQTEEDRKNILRLQDLVDKLQAKV KSYKRQAEEAEEQSNTNLSKFRKLQ HELEEAEERADIAESQVNKLRVKSRE VHTKVISEE SEQ ID Q9TV62 Myosin-4/Sus MSSDQEMAIFGEAAPYLRKSEKERIE NO: 48 (UniProtKB) scrofa AQNKPFDAKTSVFVAEPKESFVKGT VQSREGGKVTVKTEAGATLTVKEDQ VFPMNPPKFDKIEDMAMMTHLHEPA VLYNLKERYAAWMIYTYSGLFCVTV NPYKWLPVYNAEVVTAYRGKKRQE APPHIFSISDNAYQFMLTDRENQSILI TGESGAGKTVNTKRVIQYFATIAVTG EKKKEEPTPGKMQGTLEDQIISANPL LEAFGNAKTVRNDNSSRFGKFIRIHF GTTGKLASADIETYLLEKSRVTFQLK AERSYHIFYQIMSNKKPELIEMLLITT NPYDYAFVSQGEITVPSIDDQEELMA TDSAIEILGFTSDERVSIYKLTGAVM HYGNLKFKQKQREEQAEPDGTEVA DKAAYLQGLNSADLLKALCYPRVK VGNEFVTKGQTVQQVYNAVGALAK AVYDKMFLWMVTRINQQLDTKQPR QYFIGVLDIAGFEIFDFNSLEQLCINFT NEKLQQFFNHHMFVLEQEEYKKEGI EWEFIDFGMDLAACIELIEKPMGIFSI LEEECMFPKATDTSFKNKLYEQHLG KSNNFQKPKPAKGKAEAHFSLIHYA GTVDYNITGWLDKNKDPLNETVVGL YQKSSVKTLAFLFAERQSSEEGGTKK GGKKKGSSFQTVSALFRENLNKLMT NLRSTHPHFVRCIIPNETKTPGAMEH ELVLHQLRCNGVLEGIRICRKGFPSRI LYADFKQRYKVLNASAIPEGQFIDSK KASEKLLGSIDIDHTQYKFGHTKVFF KAGLLGTLEEMRDEKLAQLITRTQA MCRGFLMRVEFRKMMERRESIFCIQ YNIRAFMNVKHWPWMKLYFKIKPL LKSAETEKEMANMKEEFEKTKEDLA KSEAKRKELEEKMVALMQEKNDLQ LQVQAEADGLADAEERCDQLIKTKI QLEAKIKEVTERAEDEEEINAELTAK KRKLEDECSELKKDIDDLELTLAKVE KEKHATENKVKNLTEEMAGLDENIA KLTKEKKALQEAHQQTLDDLQAEED KVNTLTKAKTKLEQQVDDLEGSLEQ EKKLRMDLERAKRKLEGDLKLAQES TMDIENDKQQLDEKLKKKEFEMSNL QSKIEDEQALAMQLQKKIKELQART EELEEEIEAERASRAKAEKQRSDLSR ELEEISERLEEAGGATSAQIEMNKKR EAEFQKMRRDLEEATLQHEATAAAL RKKHADSVAELGEQIDNLQRVKQKL EKEKSELKMEIDDLASNMETVSKAK GNLEKMCRTLEDQLSEVKTKEEEHQ RLINELSAQKARLQTESGEFSRQLDE KEALVSQLSRGKQAFTQQIEELKRQL EEETKAKSALAHAVQSSRHDCDLLR EQYEEEQEAKAELQRAMSKANSEVA QWRTKYETDAIQRTEELEEAKKKLA QRLQDAEEHVEAVNAKCASLEKTK QRLQNEVEDLMLDVERSNAACAAL DKKQRNFDKILAEWKHKYEETQAEL EASQKESRSLSTELFKVKNAYEESLD QLETLKRENKNLQQEISDLTEQIAEG GKHIHELEKVKKQIEQEKSELQAALE EAEASLEHEEGKILRIQLELNQVKSEI DRKIAEKDEEIDQMKRNHIRVVESM QSTLDAEIRSRNDALRIKKKMEGDL NEMEIQLNHANRQATEAIRNLRNTQ GVLKDTQLHLDDAIRGQDDLKEQLA MVERRANLMQAEIEELRASLEQTER SRRVAEQELLDASERVQLLHTQNTS LINTKKKLETDISQIQGEMEDIVQEA RNAEEKAKKAITDAAMMAEELKKE QDTSAHLERMKKNMEQTVKDLQHR LDEAEQLALKGGKKQIQKLEARVRE LENEVENEQKRNVEAVKGLRKHERR VKELTYQTEEDRKNVLRLQDLVDKL QSKVKAYKRQAEEAEEQSNVNLSKF RKLQHELEEAEERADIAESQVNKLR VKSREVHTKVISEE SEQ ID P02565 Myosin-1B/ MATDADMAIFGEAAPYLRKSEKERI NO: 49 (UniProtKB) Gallus gallus EAQNKPFDAKSSVFWHAKESYVKS TIQSKESGhKVTVKTEGGETLTVKED QIFSMNPPKYDKIEDMAMMTHLHEP AVLYNLKERYAAWMIYTYSGLFCVT VNPYKWLPVYNPEVVLAYRGKKRQ EAPPHIFSISDNAYQFMLTDRENQSIL ITGESGAGKTVNTKRVIQYFATIAAS GDKKKEEQPAGKMQGTLEDQIISAN PLLEAFGNAKTVRNDNSSRFGKFIRI HFGATGKLASADIETYLLEKSRVTFQ LKAERSYHIFYQIMSNKKPELIEMLLI TTNPYDYQYVSQGEITVPSINDQEEL MATDSAIDILGFTPDEKTAIYKLTGA VMHYGNLKFKQKQREEQAEPGGTE VADKAAYLMGLNSADLLKALCYPR VKVGNEYVTKGQTVQQVYNSVGAL AKSVFEKMFLWMVVRINQQLDTKQ PRQYFIGVLDIAGFEIFDFNSLEQLCI NFTNEKLQQFFNHHMFVLEQEEYKK EGIEWEFIDFGMDLAACIELIEKPMGI FSILEEECMFPKATDTSFKNKLYDQH LGKSNNFQKPKPGKGKAEAHFSLVH YAGTVDYNITGWLEKNKDPLNETVV GLYQKSSLKTLALLFASVGGAEAES GAGGKKGGKKKGSSFQTVSALFREN LNKLMSNLRSTHPHFVRCLIPNETKT PGAMEHELVLHQLRCNGVLEGIRICR KGFPIRILYADFKQRYKVLNASAIPE GQFIDSKKASEKLLGSIDVDHTQYKF GHTKVFFKAGLLGLLEEMRDEKLAQ LITRTQARCRGFLMRVEFKKMMERR ESIFCIQYNVRAFMNVKHWPWMKLF FKIKPLLKSAESEKEMANMKEEFEKT KEELAKSEAKRKELEEKMVSLLQEK NDLQLQVQAEADGLADAEERCDQLI KTKIQLEAKIKELTERAEDEEEMNAE LTAKKRKLEDECSELKKDIDDLELTL AKVEKEKHATENKVKNLTEEMAAL DETIAKLTKEKKALQEAHQQTLDDL QAEEDKVNTLTKAKTKLEQQVDDLE GSLEQEKKLRMDLERAKRKLEGDLK MTQESTMDLENDKQQLDEKLKKKD FEISQIQSKIEDEQALGMQLQKKIKEL QARIEELEEEIEAERTSRAKAEKHRA DLSRELEEISERLEEAGGATAAQIDM NKKREAEFQKMRRDLEEATLQHEAT AAALRKKHADSTADVGEQIDNLQRV KQKLEKEKSELKMEIDDLASNMESV SKAKANLEKMCRSLEDQLSEIKTKEE EQQRTINDISAQKARLQTESGEYSRQ VEEKDALISQLSRGKQAFTQQIEELK RHLEEEIKAKKCPAHALQSARHDCD LLREQYEEEQEAKGELQRALSKANS EVAQWRTKYETDAIQRTEELEEAKK KLAQRLQDAEEHVEAVNSKCASLEK TKQRLQNEVEDLMIDVERSNAACAA LDKKQKNFDKILSEWKQKYEETQAE LEASQKESRSLSTELFKMKNAYEESL DHLETLKRENKNLQQEISDLTEQIAE GGKAIHELEKVKKQIEQEKSELQTAL EEAEASLEHEEGKILRVQLELNQVKS DIDRKIAEKDEEIDQLKRNHLRVVDS MQSTLDAEIRSRNEALRLKKKMEGD LNEIEIQLSHANRQAAEAQKNLRNTQ GVLKDTQIHLDDALRSQEDLKEQVA MVERRANLLQAEIEELRAALEQTERS RKVAEQELLDASERVQLLHTQNTSLI NTKKKLESDISQIQSEMEDTIQEARN AEEKAKKAITDAAMMAEELKKEQD TSAHLERMKKNLDQTVKDLQHRLD EAEQLALKGGKKQIQKLEARVRELE GEVDAEQKRSAEAVKGVRKYERRV KELTYQSEEDRKNVLRLQDLVDKLQ MKVKSYKRQAEEAEELSNVNLSKFR KIQHELEEAEERADIAESQVNKLRAK SREIGKKAESEE SEQ ID Q9TV61 Myosin-1/Sus MSSDQEMAIFGEAAPYLRKSEKERIE NO: 50 (UniProtKB) scrofa AQNKPFDAKTSVFVAEPKESFVKGT VQSREGGKVTVKTEAGATLTVKEDQ VFPMNPPKFDKIEDMAMMTHLHEPA VLYNLKERYAAWMIYTYSGLFCVTV NPYKWLPVYNAEVVTAYRGKKRQE APPHIFSISDNAYQFMLTDRENQSILI TGESGAGKTVNTKRVIQYFATIAVTG EKKKEEPTSGKMQGTLEDQIISANPL LEAFGNAKTVRNDNSSRFGKFIRIHF GTTGKLASADIETYLLEKSRVTFQLK AERSYHIFYQIMSNKKPELIEMLLITT NPYDYAFVSQGEITVPSIDDQEELMA TDSAIEILGFTSDERVSIYKLTGAVM HYGNLKFKQKQREEQAEPDGTEVA DKAAYLQGLNSADLLKALCYPRVK VGNEFVTKGQTVQQVYNAVGALAK AVYDKMFLWMVTRINQQLDTKQPR QYFIGVLDIAGFEIFDFNSLEQLCINFT NEKLQQFFNHHMFVLEQEEYKKEGI EWEFIDFGMDLAACIELIEKPMGIFSI LEEECMFPKATDTSFKNKLYEQHLG KSNNFQKPKPAKGKVEAHFSLIHYA GTVDYNITGWLDKNKDPLNETVVGL YQKSSVKTLAFLFTGAAGADAEAGG GKKGGKKKGSSFQTVSALFRENLNK LMTNLRSTHPHFVRCIIPNETKTPGA MEHELVLHQLRCNGVLEGIRICRKGF PSRILYADFKQRYKVLNASAIPEGQFI DSKKASEKLLGSIDIDHTQYKFGHTK VFFKAGLLGLLEEMRDEKLAQLITRT QARCRGFLARVEYQKMVERRESIFCI QYNIRAFMNVKHWPWMKLYFKIKP LLKSAETEKEMANMKEEFEKTKESL AKAEAKRKELEEKMVALMQEKNDL QLQVQAEADSLADAEERCDQLIKTKI QLEAKIKEVTERAEDEEEINAELTAK KRKLEDECSELKKDIDDLELTLAKVE KEKHATENKVKNLTEEMAGLDETIA KLTKEKKALQEAHQQTLDDLQAEED KVNTLTKAKTKLEQQVDDLEGSLEQ EKKLRMDLERAKRKLEGDLKLAQES TMDIENDKQQLDEKLKKKEFEMSNL QSKIEDEQALAMQLQKKIKELQARIE ELEEEIEAERASRAKAEKQRSDLSRE LEEISERLEEAGGATSAQIEMNKKRE AEFQKMRRDLEEATLQHEATAATLR KKHADSVAELGEQIDNLQRVKQKLE KEKSEMKMEIDDLASNMETVSKAK GNLEKMCRTLEDQLSELKTKEEEQQ RLINDLTAQRARLQTESGEYSRQLDE KDTLVSQLSRGKQAFTQQIEELKRQL EEEIKAKSALAHAVQSSRHDCDLLRE QYEEEQEAKAELQRAMSKANSEVA QWRTKYETDAIQRTEELEEAKKKLA QRLQDAEEHVEAVNAKCASLEKTK QRLQNEVEDLMIDVERSNAACAALD KKQRNFDKILAEWKQKYEETHAELE ASQKESRSLSTELFKVKNAYEESLDQ LETLKRENKNLQQEISDLTEQIAEGG KRIHELEKIKKQVEQEKSEIQAALEE AEASLEHEEGKILRIQLELNQVKSEV DRKIAEKDEEIDQLKRNHVRVVESM QSMLDAEIRSRNDAIRLKKKMEGDL NEMEIQLNHANRMAAEALRNYRNT QGILKDTQIHLDDALRSQEDLKEQLA MVERRANLLQAEIEELRATLEQTERS RKVAEQELLDASERVQLLHTQNTSLI NTKKKLETDISQIQGEMEDIIQEARN AEEKAKKAITDAAMMAEELKKEQD TSAHLERMKKNLEQTVKDLQHRLDE AEQLALKGGKKQIQKLEARVRELEG EVESEQKRNVETVKGLRKHERRVKE LTYQTEEDRKNILRLQDLVDKLQAK VKSYKRQAEEAEEQSNVNLSKFRKL QHELEEAEERADIAESQVNKLRVKS REVHTKIISEE SEQ ID Q9DGM4 Fast myosin MASSDAEMAAFGEAAPYHRKSEKE NO: 51 (UniProtKB) heavy chain RIEAQNKPFDAKSSVFVAHPKESFVK isoform 3/Gallus GTIQSRETGKVTVKTEGGETLTVKED gallus QVFSMNPPKYDKIEDMAMMTHLHE PAVLYNLKERYAAWMIYTYSGLFCV TVNPYKWLPVYNPEVVLAYRGKKR QEAPPHIFSISDNAYQFMLTDRENQSI LITGESGAGKTVNTKRVIQYFATIAA SGEKKKEEQSGKMQGTLEDQIISANP LLEAFGNAETVRNDNSSRFGKFIRIH FGATGKLASADIETYLLEKSRVTFQL KAERSYHIFYQIMSNKKPELIDMLLIT TNPYDYHFVSQGEITVPSIDDQEELM ATDSAIDILGFTADEKTAISKLTGAV MHYGNLKFKQKQREEQAEPDGTEV ADKAAYLMGLNSADLLKALCYPRV KVGNEYVTKGQTVQQVHNAVGALA KAVYEKMFLWMVVRINQQLDTKQP RQYFIGVLDIAGFEIFDFNSFEQLCINF TNEKLQQFFNHHMFVLEQEEYKKEG IEWTFIDFGMDLAACIELIEKPMGIFSI LEEECMFPKATDTSFKNKLYDQHLG KSSNFQKPKPAKGKAEAHFSLVHYA GTVDYNITGWLEKNKDPLNETVIGL YQKSSVKTLALLFATYGGADAEAGG GGKKGGKKKGSSFQTVSALFRENLN KLMTNLRSTHPHFVRCIIPNETKTPG AMEHELVLHQLRCNGVLEGIRICRK GFPSRVLYADFKQRYKVLNASAIPEG QFIDSKKASEKLLSSIDVDHTQYKFG HTKVFFKAGLLGLLEEMRDEKLAQL ITRTQARSRGFLMRVEYQRMVERRE SIFCIQYNVRSFMNVKHWPWMKLFF KIKPLLKSAESEKEMANMKEEFEKT KEELAKSEAKRKELEEKMVKLVQEK NDLQLQVQAEADSLADAEERCDQLI KTKIQLEAKIKEVTERAEDEEEINAEL TAKKRKLEDECSELKKDMDDLELTL AKVEKEKHATENKVKNLTEEMAAL DETIVKLTKEKKALQEAHQQTLDDL QAEEDKVNTLTKAKTKLEQQVDDLE GSLEQEKKLRMDLERAKRKLEGDLK LAHDSIMDLENDKQQLDEKLKKKDF EISQIQSKIEDEQALGMQLQKKIKEL QARIEELEEEIEAERTSRAKAEKHRA DLSRELEEISERLEEAGGATAAQIDM NKKREAEFQKMRRDLEEATLQHEAT AAALRKKHADSTAELGEQIDNLQRV KQKLEKEKSELKMEIDDLASNMESV SKAKANLEKMCRTLEDQLSEIKTKE EEHQRMINDLNTQRARLQTEAGEYS RQVEEKDALISQLSRGKQAFTQQIEE LKRHLEEEIKAKNALAHALQSARHD CDLLREQYEEEQEAKGELQRALSKA NSEVAQWRTKYETDAIQRTEELEEA KKKLAQRLQDAEEHVEAVNAKCAS LEKTKQRLQNEVEDLMIDVERANAA CAALDKKQKNFDKILAEWKQKYEE TQAELEASQKESRSLSTELFKMKNA YEESLDHLQTLKRENKNLQQEISDLT EQIAEGGKAIHELEKVKKQIEQEKSEI QAALEEAEASLEHEEGKILRLQLELN QVKSEIDRKIAEKDEEIDQLKRNHLRI VESLQSSLDAEIRSRNEALRLKKKME GDLNEMEIQLSHANRVAAEAQKNLR NTQAVLKDTQIHLDDALRTQEVLKE QVAMVERRANLLQAEIEELRAALEQ TERSRKVAEQELMDASERVQLLHTQ NTSLINTKKKLETDIAQIQSEMEDTIQ EARNTEEKAKKAITDAAMMAEELK KEQDTSAHLERMKKNLDQTVKDLQ HRLDEAEQLALKGGKKQIQKLEARV RELEGEVDAEQKRSAEAVKGVRKYE RRVKELTYQSEEDLKNILRLQDLVD KLQMKVKSYKRQAEEAEELSNVNLS KFRKIQHELEEAEERADIAESQVNKL RVKSREFHSKKIEEEE SEQ ID P13538 Myosin heavy MASPDAEMAAFGEAAPYLRKSEKER NO: 52 (UniProtKB) chain, skeletal IEAQNKPFDAKSSVFVVHPKESFVKG muscle, TIQSKEGGKVTVKTEGGETLTVKED adult/Gallus QVFSMNPPKYDKIEDMAMMTHLHE gallus PAVLYNLKERYAAWMIYTYSGLFCV TVNPYKWLPVYNPEVVLAYRGKKR QEAPPHIFSISDNAYQFMLTDRENQSI LITGESGAGKTVNTKRVIQYFATIAA SGEKKKEEQSGKMQGTLEDQIISANP LLEAFGNAKTVRNDNSSRFGKFIRIH FGATGKLASADIETYLLEKSRVTFQL PAERSYHIFYQIMSNKKPELIDMLLIT TNPYDYHYVSQGEITVPSIDDQEELM ATDSAIDILGFSADEKTAIYKLTGAV MHYGNLKFKQKQREEQAEPDGTEV ADKAAYLMGLNSAELLKALCYPRV KVGNEFVTKGQTVSQVHNSVGALA KAVYEKMFLWMVIRINQQLDTKQPR QYFIGVLDIAGFEIFDFNSFEQLCINFT NEKLQQFFNHHMFVLEQEEYKKEGI EWEFIDFGMDLAACIELIEKPMGIFSI LEEECMFPKATDTSFKNKLYDQHLG KSNNFQKPKPAKGKAEAHFSLVHYA GTVDYNISGWLEKNKDPLNETVIGL YQKSSVKTLALLFATYGGEAEGGGG KKGGKKKGSSFQTVSALFRENLNKL MANLRSTHPHFVRCIIPNETKTPGAM EHELVLHQLRCNGVLEGIRICRKGFP SRVLYADFKQRYRVLNASAIPEGQF MDSKKASEKLLGSIDVDHTQYRFGH TKVFFKAGLLGLLEEMRDDKLAEIIT RTQARCRGFLMRVEYRRMVERRESI FCIQYNVRSFMNVKHWPWMKLFFKI KPLLKSAESEKEMANMKEEFEKTKE ELAKSEAKRKELEEKMVVLLQEKND LQLQVQAEADSLADAEERCDQLIKT KIQLEAKIKEVTERAEDEEEINAELTA KKRKLEDECSELKKDIDDLELTLAK VEKEKHATENKVKNLTEEMAVLDE TIAKLTKEKKALQEAHQQTLDDLQV EEDKVNTLTKAKTKLEQQVDDLEGS LEQEKKLRMDLERAKRKLEGDLKLA HDSIMDLENDKQQLDEKLKKKDFEI SQIQSKIEDEQALGMQLQKKIKELQA RIEELEEEIEAERTSRAKAEKHRADLS RELEEISERLEEAGGATAAQIEMNKK REAEFQKMRRDLEEATLQHEATAAA LRKKHADSTAELGEQIDNLQRVKQK LEKEKSELKMEIDDLASNMESVSKA KANLEKMCRTLEDQLSEIKTKEEQN QRMINDLNTQRARLQTETGEYSRQA EEKDALISQLSRGKQGFTQQIEELKR HLEEEIKAKNALAHALQSARHDCEL LREQYEEEQEAKGELQRALSKANSE VAQWRTKYETDAIQRTEELEEAKKK LAQRLQDAEEHVEAVNAKCASLEKT KQRLQNEVEDLMVDVERSNAACAA LDKKQKNFDKILAEWKQKYEETQTE LEASQKESRSLSTELFKMKNAYEESL DHLETLKRENKNLQQEIADLTEQIAE GGKAVHELEKVKKHVEQEKSELQAS LEEAEASLEHEEGKILRLQLELNQIKS EIDRKIAEKDEEIDQLKRNHLRIVES MQSTLDAEIRSRNEALRLKKKMEGD LNEMEIQLSHANRMAAEAQKNLRNT QGTLKDTQIHLDDALRTQEDLKEQV AMVERRANLLQAEVEELRGALEQTE RSRKVAEQELLDATERVQLLHTQNT SLINTKKKLETDIVQIQSEMEDTIQEA RNAEEKAKKAITDAAMMAEELKKE QDTSAHLERMKKNMDQTVKDLHVR LDEAEQLALKGGKKQLQKLEARVRE LEGEVDSEQKRSAEAVKGVRKYERR VKELTYQCEEDRKNILRLQDLVDKL QMKVKSYKRQAEEAEELSNVNLSKF RKIQHELEEAEERADIAESQVNKLRV KSREIHGKKIEEEE SEQ ID P16419 Myosin-binding MPEPSKAAPKKEAKKKEEKKEEKKE NO: 53 (UniProtKB) protein C, fast- APPPQEHKDEAPDDVHPPETPDPEGL type/Gallus FLSKPQNVMVESGRDVTVSARVAGA gallus ALPCAPAVKWFKGKWAELGDKSAR CRLRHSVDDDKVHTFELTITKVAMG DRGDYRCEVTAKEQKDSCSFSIDVE APRSSEGNVLQAFKRTGEGKDDTAG ELDFSGLLKKREVQVEEKKKKKDED DQFPPEIWELLKGVTKKSEYERIAFQ YGITDLRGMLKRLKKVHVEPKKSEA FIRKLDPAYQVDKGNKIKLVVELSDP DLPLKWYKNGQLLKPSTKYVFENVG LKRILTIHKCSLADDAAYECRVNDEK CFTEVFVKEPPVTVVRGLEDQQVVV GDRVVLEAEVSEEGAQVMWLKDGV DVTRDDAFKYRFKKDGKKHFLIINE AELSDSAHYKIMTNGGESEAELSVEE KQLEVLQDMADLTVKASEQAVFKC EVSDEKVTGRWFRNGVEVKPSKRIHI SHNGRFHKLVIDDVRPEDEGDYTFIP DGYALSLSAKLNFLEIKVEYVPKQEP PKIHLDCSGKAAENTIVVVAGNKVR LDVPISGEPAPTVTWKRGDQLFTATE GRVHIDSQADLSSFVIESAERSDEGR YCITVTNPVGEDSATLHVRVVDVPD PPQSVRVTSVGEDWAVLSWEAPPFD GGMPITGYLMERKKKGSMRWMKLN FEVFPDTTYESTKMIEGVFYEMRVFA VNAIGVSQPSLNTQPFMPIAPTSEPTH VVLEDVTDTTATIKWRPPERIGAGG VDGYLVEWCREGSNEWVAANTELV ERCGLTARGLPTGERLLFRVISVNMA GKSPPATMAQPVTIREIVERPKIRLPR HLRQTYIRRVGEQVNLVIPFQGKPRP QVTWSREGGALPAEVQTRTSDVDSV FFIRSAARPLSGNYEMRVRIDNMEDC ATLRLRVVERPGPPQAVRVMEVWG SNALLQWEPPKDDGNAEISGYTVQK ADTRTMEWFTVLEHSRPTRCTVSEL VMGNEYRFRVYSENVCGTSQEPATS HNTARIAKEGLTLKMVPYKERDLRA APQFLTPLVDRSVVAGYTVTLNCAV RGHPKPKVTWLKNSVEIGADPKFLS RHGLGVLSLLIRRPGPFDGGTYGCRA VNEMGEATTECRLDVRVPQ SEQ ID E1BNV1 Myosin binding MPEAKPAAKKAPAGKDAAAKPAPK NO: 54 (UniProtKB) protein C, fast EATPQEAPAAPPTEAPPEDQSPTAEE type/Bos taurus PTGVFLKKPDSVSVETGKDTVIVAKL NGKELPAKPAVKWFKGKWLELGSK SGARFSFKESHDAASNVYTIELHITK VVLGDRGDYRIEVKAKDFCDSCAFN IDVEAPRQDSAGQSLESFKRSGEAKS DTAGELDFSGLLKKRQVVEEEKKKK KDDDDLGIPPEIWELLKGAKKSEYER IAFQYGITDLRGMLKRLKKAKVEVK KSAAFTKKLDPAYQVDRGNKIKLVV EISDPDLPLKWFKNGQEIKPSSKYVF ENVGKKRILTINKCTLADDAAYEVV VKDEKCFTELFVKVEPPVLIVTPLED QQVFVGDRVEMAVEVSEEGAQVM WLKDGVELTREDSFKARYRLKKDG KRHILIYSEVTMEDKGHYQVMTNGG QCEAELIVEEKQLEVLQDIADLTVKA SEQAVFKCEVSDEKVTGKWYKNGV EVRPSKRITISHTGRFHKLVIDDVRPE DEGDYTFVPDGYALSLSAKLKFLEIK VEYVPKQEPPKIHLDCSGQTSENAIV VVAGNKLRMDVSITGEPRPVATWM KEDEVFTGTEGRVRIEQRGDISSFVIE SAERGDEGRYTIKVTNPVGEDVASIL LKVVDVPDPPEAVRVTSVGEDWAV LVWEPPKYDGGRPVTGYLLERKKK GSQRWMKLNFEVFTETTYESTNMIE GILYEMRVFAAPLIQQLKTLTPQPHA PFSPTSEPQHLTVEDVTDTTTTLKWR PPDKIGAGGIDGYLIEYCVEGSDEWI PANTEPVERCGFTVKNLPTGAKITFR VVGVNIAGRSQPATLAQPVTIREIVE QPKIRLPRHLRQTYVRKVGEHLNLVI PFQVRRAKGGAPIDTSHVHVRTSDF DTVFFVRQAARSDSGEYELTVQIEN MKDTATVCIRVVEKAGPPQNVMVK EVWGTNALVEWQPPKDDGNSEITGY FVQKADKKTMEWFTVYERNRHTSC TVSDLIMGNEYYFRVYSENVCGLSD LPGVSKNTARIVKTGMTLKLPEYKE HDFRTPPKFLTPLPDRVVVAGYAAA LNCAVRGHPKPKVVWMKNKMEIRE DPKFLMTNQQGVLTLNIRRPSPFDSG TYSCRAVNELGEALAECKLEVRAPR SEQ ID Q05623 Myosin-binding MTGKTAPAAAKKAPAAKKAPAPAS NO: 55 (UniProtKB) protein H/Gallus KKAPEPAPKEKPAPTPKEGHAPTPKE gallus EHAPPPKEEHAPPPKEEHAPAPAAET PPAPEHPPDAEQPAAPAAEHAPTPTH EAAPAHEEGPPPAAPAEAPAPEPEPE KPKEEPPSVPLSLAVEEVTENSVTLT WKAPEHTGKSSLDGYVVEICKDGST DWTAVNKEPFLSTRYKIHDLASGEK VHVRVKAISASGTSDPATLEQPVLIR EITDLPRIRLPRQLRQVYVRHVGEAV NLLIPFQGKPQPQVTWTKDNQPLDTS RVNIRNTDKDTIFFIRTAQRSDSGKY QLSVRINGAEDKAILDIRVIERPGPPQ NLKLVDVWGFNVALEWSPPADNGN SEIKGYTVQKSDKKSGKWFTVLERC TRTSCTISDLIIGNTYSFRVFSENACG MSETAAVAAGVAHIKKTVYQPQKIP ERDMMEPPKFTQPLTDRATTRGYST HLFCSVRGFPQPKIIWMKNKMEIRED PKYIAMIEQGVCSLEIRKPSPFDAGV YTCKAVNPLGEASVDCKLDVKMPK SEQ ID G3X6W9 Myosin binding MTEKATSEAPACGLEETTSESAHVPL NO: 56 (UniProtKB) protein H/Bos TEPSGDTAAPQAPGGEQAPRGQQAS taurus DPQESARQPPDPAASAAPAGPAATD PALPREDVPSAPLLLAVEDVSDSSVT VSWEPPERLGRLGLQGYVLELRREG ALDWVPVNARPMMVTQQTLRNLAV GDKFFVRVAAVSSAGAGPPAVLERLI HIQETIEAPKIRVPRHLRQTYIRQVGE SINLQIPFQGNPKPQALWTHNGHALD SQRVSVRTGDQDSILFIRSAQRSDSG CYELTVQLKDLEAKAAINILVIEKPG PPRSIRLLDVWNCNATLEWTPPQDT GNTELLGYTVQKADKKTGQWFTVL ERCHPTSCTVSDLIVGNSYSFRVFSE NLCGLSASAAVTKELAHIVKTDIVAK PKSFVERDFSEAPSFTQPLADHTSTPG YSTQLSCSVRASPKPKIIWMKNKMDI QGDPKYRALSEQGVCTLEIRKPSPFD SGVYTCKAINVLGEASVDCRLEVKA SATH SEQ ID A6BM71 Connectin MTTKAPTFTQPLQSVVALEGSAATFE NO: 57 (UniProtKB) (Fragment)/Gallus AHISGFPVPEVSWYRDGQVLSAATLP gallus GVQISFSDGRAKLVIPSVTEANSGRY TIQATNGSGQATSTAELLVTAGTAPP NFSQRLQSMTARQGSQVRLDVRVTG IPTPVVKFYRDGVEIQSSPDFQILQEG DLYSLIIAEAYPEDSGTYSVNATNNV GRATSTAELLIQGEEEAVPAKKTKTI VSTAQISQTRQARIEKKIETHFDARSL TSVEMVIEGAAAQQLPHKAPPRMPP RPTSKSPTPPVITAKAQMARQQSPSP VRQSPSPVRHVRAPTPSPVRSVSPAG RISTSPIRPVKSPSPIRKAQVVTPGAE VLPPWRQEGYSATAEAQMKETRVST SATEIRTEERWEGRYGLQEQVTISGA AAGEVAAGAKEVRKEPEKTPVPTVII ATDKAKEQERISTAREEISARHEQVH VSHEQIEAGKRAEAVATVVAAVDQ ARVRSPWETEQVDETYVKKKTLEYG YKEHAVKDHEAQAEHHVATKEVKT VYVPPEKHIPAAEKKEVHVSTEIKRE TEAKIEKTIHIEHPRPRTASPHFTVSKI AVPKPDHTYEVSIAGSAMATLEKELS ATSAAQKITKPVKPPQLKPHEVKIKP ESAPPQFPFTEAAETYKAHYDVETK KEVDVSIKGEAVREDHLLLRKESEA KVTETARVPVPAEIPVTPPTLVWGLK NKTVTEGESVTLECHISGHPQPTVTW YREDYKIESSMDFQITFKAGLARLVI REAFAEDSGRFTCTATNKAGSVSTSC HLHVKVSEETETRETISEKVVTEEKS YVETKDVVMEDVSAAAEEVSGEPVP PFFIRKPVVHKLIEGGSIIFECQVGGN PKPHVLWKKGGVPLTTGYRYKVSY KRETGECKLEISMTFADDAGEYTIVI RNKFGEASATVSLLEEADYEAYIKSQ QEMMYQTQVTAYVQEPKVAEVAPPI SYGDFDKEYEKEQALIRKKMAKDTV MVRTFVEDEEFHISSFEERLIKEIELRI IKTTLDELLEEDGEEMMIDISESEAIG AGFDLRLKNYRTFEGTGVTFHCKTT GYPLPKIAWYKDGKRIRHGERYHME VLQDGSASLRLPVVLPEDEGIYTVFA SNMKGNAICSAKLYVEPVAPTATPG YMPGPEVMRRYRSISPRSPSRSPARS SPSCSPARRLDETDEGQLERLYKPVF VLKPTSVKCSQGQTARFDLKVVGRP MPETYWFHNGQQVVNDYTHKIVIKE DGTQSLIIVPAMPEDSGEWAVIAQNR AGKASVSVTLSVEAKEDLVRPRFVE RLRNVSVKEGSRLHMAVKATGNPNP DIVWLKNSDIIVPHKYPRIRIEGTKGA AALNIESTARQDAAWYTATAINKAG RDTTRCKVNVEVEHAEPEPERRLIIP KGTYKAKEIAAPELEPLHLRYGQEQ WEEGDLYDKEKQQKPFFKKKLTSLR LKQFGPAHFECRLTPIGDPTMVVEW LHDGKPLEAANRLRMINEFGYCSLD YGVAYSRDSGVITCRATNKYGTDHT SATLIVKDEKSLVEESQLPEGRRGMQ RIEELERMAHEGALPAVAVDQKEKQ KPELVLVPEPARVLEGETARFRCRVT GYPLPKVNWYLNSQLIRKSKRFRLR YDGIHYLDIVDCKSYDTGEVKVTAE NPEGFIEHKVKLEIQQREDFRSVLRR APEPRHEPVVTEPGKLLFEVQKIDKP AEATTKEVVKLKRAERITHEKLSEES EELRSKFKRRTEEGYYEAITAVELKS RKKDESYEEMLKKTKEELLHWTKEI PEEEKKALPPEGKITIPTFKPEKVELS PSMEAPKIFERIQSQTVAQGTDAHFR VRVVGKPDPECQWFRNGVQIERTDR IYWYWPEDNVCELVIRDVTADDSAS IMVKAVNIAGETSSHAFLLVQAKQLI SFIQNLQDVVAKERDSMATFECETSE PFIKVKWFKNGIEIHSGEKYRMHSDR KAHFLSVLAVEMSDADDYSCALVED ESVKTTAKLIVEGAVVEFIKELEDVE VPESFTGELECEVSPEDIEGKWYHGD VELSSNHKYVLASRRGRRILTIKDVN KDDQGEYSFVVDGKRTHCKLKMKP RPMTILQGLTDQKVCEGDIVQLEVK VSVENVEGVWMKDGHEIQSSDRIHI VLDKQSHMLLIEDATQEDSGTYSFSI PGLELSTTGQVTVYSVEIIVPLKDVH VVEGTKAILECKVSAPDVTSSKWYL NDHQIKPDERVQAVCKGTKQRLVIT RTHASDEGHYKLMVGKVETSCNVT VEEIEIIRGLHDITCTETQNVSFEVELS HSGIDVIWHFKGQEIKAGPKYKIEAR GKIYKLTVVKMMKDDEGEYVFYAG GKKTSGKLIVAGGAISKPLADLTVAE SQRAVFECEVANPESEGQWLKNGKP LPMTDQYRAETDGVKRRLNIPAAKM DDMGEYSYEIASSKTSAKLHVEAVKI KKTLKNLTVTETQEAVFSVELSHPD VKGALWIKNGVELESNDKYEISVKG TVHTLKIKHCVVTDESVYSFKLGKIG ANARLHVETVKIIKKPKDVTALENA VVSFELSVSHDTVPVRWFHKNVELK QSDKYKMISQRKVHKLMLHNISPAD AGEYTAFVGQLECKAKLFVETIHITK TMKSIEIPETKTASFQCEVSHFNVPSV WLKNGVEIEMSEKFKIVVQGKLHQL NIMNTSSEDSAEYTFVCGNDRVSATL TVKPILITSMLEDINAEEKDTITFEVT VNYEGISYKWLKNGVEIKSTDKCQIR TKKLTHSLSIRNVHFGDAAEYSFVAG KAASSATLYVEARHIEFRKHIKDIKV VEKKRAIFECEISEPDVQVQWMKDG QELQIGDRMKIQREKYVHRLIIPSTK MSDAGQYTVVAGGNTSSANLIVEGR DVRIRSIRKEIQVIERQRAEIEFEVNE DDIEPQWYKDGIEINFHYEERYSYVV ERRIHRMSIFETTYSDAGEYTFVAGR NRSSVVLYVNAPEPPQIIQELQPTTVE SGKPARFCAIISGKPQPKVSWYKDDQ QLSPGFKCKFLHDAQEYTLLLIETFPE DSAVYTCEAKNDYGVATTSASLSVE IPEVVSPELEVPVYPPAVIVPLRDAVT SEGQSARFQCRVTGTDLKVSWYSKD REIKPSRFFRMTQFEDTYQLEIAEAYP EDEGTYTFVASNSVGQVTSTAILKLE APEKIMYEKLEEEIEMEVKVAPILRR RLEPLEVAVNHVAKFTCEVETTPNV KFQWYKAGREIYDGDKYSIRSSNYL STLEIPRPQVVDCGEYSCKASNQHGS VSSTAFLTVTEPPRFIKKLDSSRLVKQ HDSTRYECKVGGSPEIKVTWYKGET EIHPSEKYSMSFVDSVAVLEMHNLS VEDSGDYSCEAQNPAGSASTSTSLK VKAPPAFTKKPHPVQTLKGSDVHLE CELQGTPPFQISWYKDKREIRSSKKY KVMSENYLASIHILNVDTADVGEYH CKAVNDVGSDSCIGSVTLRAPPTFVK KLSDVTVVVGETIELQAAVEGAQPIS VLWLKDKGEIIRESENLWISYSENVA SLKIGNAEPTNAGKYICQIKNDAGFQ ECFAKLTVLEPAVIVEKPGPVKVTAG DSCTLECTVDGTPELTARWFKDGNE LSTDHKYKISFFNKVSGLKILNAGLE DSGEYTFEVKNSVGKSSCTASLQVS DRIMPPSFTRKLKETYGQLGSSAVLE CKVYGSPPILVSWFHDGQEITSGDKY QATLTDNTCSLKVNGLQESDMGTYS CTATNVAGSDECSAFLSVREPPSFVK KPEPFNVLSGENITFTSIVKGSPPLEV KWFRGSIELAPGHKCNITLQDSVAEL ELFDVQPLQSGDYTCQVSNEAGKISC TTHLFVKEPAKFVMKVNDLSVEKGK NLILECTYTGTPPISVTWKKNGVILK HSEKCSITTTETSAILEIPNSKLEDQG QYSCHIENDSGQDNCHGAITILEPPYF VTPLEPVQVTVGDSASLQCQVAGTP EMIVSWYKGDTKLRGTATVKMHFK NQVATLVFSQVDSDDSGEYICKVEN TVGEATSSSLLTVQERKLPPSFTRKL RDVHETVGLPVTFDCGIAGSEPIEVS WFKDNVRVKEDYNVHTSFIDNVAIL QILKTDKSLMGQYTCTASNAIGTASS SGKLVLTEGKTPPFFDTPITPVDGIIG ESADFECHISGTQPIRVTWAKDNQEI RTGGNYQISYVENTAHLTILRVDRG DSGKYTCYASNEVGKDSCTAQLNV KERKTPPTFTRKLSEAVEETEGNELK LEGRVAGSQPLTVSWYKNNQEVHSS PHCEISFKNNTLLLHIKSVGQSDAGL YTCKVSNEAGSVLCTSSVVIREPKKP PVFDQPLQPAATEEGDTLQLSCHVR GSEPIRIQWLKAGREIRASERCSFSFA NGVALLELAAVTKSDSGEYVCKASN VAGTDTCRSKVTVKEKAALVSAAK KADIEGKLYFVSEPQSIKVMEKTVAT FIAKVGGDPIPNVKWMKGKWRQLN QGGRIIIQQRGDEAKLEIKDTTKTDS GLYKCVAFNQHGEIERSVNLQVEER KQEVVEEDVRGKLKRIPTKKKEDEE QTIDILELLKNVDPKEYEKYARMYGI TDFRGLLQAFELLKQTREEESHRLEI ELTEKAQKEDQEFEELVAFIQQRLTQ TEPVTLIRDIENQTVLTDEDAIFECEI KINYPEIKLSWYKGTQKLDSSDKYKI KIEGDRHILKIKNCQLEDQGNYRIVC GPHIASARLTVIEPAVERHLHDTTFK EGNTCTLSCQFSIPNAKSQWYRNGRP IKIGGRYSTQVSDKVHKLIIKDVRTE DQGQYTCKLDNLETTADLTIEAEPIQ FTKSIQNIVVSEHQSATFECEVSFDDA VVTWYKGPTELTESPKYSFRSEGRC HYMTIHNVTAEDEGVYSVIARLEPR GEARSTAELYLVTKEIKLELKPPDVP DAKVAVPPQKPAEAAPIPILLPLIPTP EEKKPAEKKVPVKKVSKKVVKKGP KEIPPPEVPEILPEKPKEVKITSMARR EEIHEEKMEIYEKPKKVYEEWEEDY GEDHDYYFKEEGYDEGEEEWEETY DKREVAYEEEQIIHEEVVEVPKKPVP ERKPPAITAEKKEKKEVTVHKVPDV PKKIPEEKVPITVPKKPEPTPAKEPEV HKIIEEKIKVSEPKKPEVPPAKVPEVP KKVVKEEVSVEVPKKPEPPPAKVPE VPKKVVPEEKVHVEVPKKAEPPPPK VLRKKPEEEEPKPEKEPKPEVKPVPT PVEKIKKPEVPEVPKKKTEIPVIKKEE KHVPEPPKEPKPAAISVPGPEPKPAV ALKSPKPAAEPAPAITTAPVTTPVVG KKAEAKPTKEETPKGISPVKAKKTPS PADAERRKLRPGSGGEKPPEEPPFTY QLKAVPLKFVKEMKDIVLKEAESVG SSAIFEVLISPSTAITSWMKDGSNIRES PKHKFIADGKDRKLHIIDVQLSDAGE YTCVLRLGNKEKTSTAKLIVEELPVR FVKTLEEEVTVVKGQPLYLTCELNK ERSVVWRRDGKIIKDKPGKFALGIIG LSHSLTITDSDDSDAGTYTVTVEDSE LSCSSCVKVVEVIRDWLVKPIRDQH VKPKGTATFTCDIVKDTPNIKWFKG DEEIPAEPTDKTEILKEGNKIFLKIKN AGPADIGEYSVEVEGRRYPAKLTLG EREVELLKPLEDVTVYEKETANFDTE ISEEDIPGEWKLKGEILRPSPTCEIKAE GGKRFLTLHKVKLEQAGEVLYQALN AVTTAILTVKEIELDFAVPLKDVTVP EKRQARFECVLTREANVIWSKGTDIL KIGEKFDIIADGKKHILVINDSQFDDE GEYTAEVEGKKSTARLFVEGVRLKFI TPLQDQTVKEGETAYFQFELSHEGM LVKWYKNDKRLHTSRTVFITSEGKV HKLEMREVTLDDISEIKAVVKDMNT QANLKVLEADPYFTVKLQDYSALEK DDITLQCELSKDVPVKWYKDGEELV ASSRISIKTDGLRRILTIKKATEGDKG VYECSCGTDKTNCNIGVEPRLIKVER PLYGVEVFVGETARFEIEISEPDVHPI WKLKGETLTPSPECEIIEDGKKHILIL HQGRLDMTGEVSFQAANAKSAANL KVKELPLIFITPLSDVKVFEKDEAKFE CEVSREPKTFRWLKGTQEITPDERFEI ISDGTKHALIIKSVAFDDEAKYMFEA EDKRTSAKLIIEGIRLKFITPLKDVTK KERETAVFTVELSHENIPVVWFKND QRLHTSKVVSMTDDGKFHTLTIKDL TIDDTSQIRVEAMGKSSEAKLTVLEG DPYFTGKLQDYTAVEKDEVILQCEIS KADAPVKWMKDGKPITPSKNVVIKA DGKKRILILKKALKKDIGQYTCDCGT DQTSANLNIEDRDIEIIRPLYSVEVIET ETARFDIEISEEGVHGNWKLKGEPLT ESPDCEIKEEGKKHFLTLYNVRLDQA GGVDFQAANAKSGAHLRVKPRVIGL LRPLKDVTVTAGESATFDCELSYEGI PVEWYLQGKKLEPSDKVVTRAEGR AHTLILRDVKLTDAGEVSLTAKDFRT QANLFVKEPPVEFTKPLEDQTVEEEA TAILECEVSRENAKVKWFKNGEEIH KTKKYDIISEGRVRKLIIHGCTLDDA KTYTCDAKDFKTSCFLNVEPLRVEFL RPLTDLEVKEKRICSVLNVKFLAQGV KVKWFKDGIEIEKAEYDIISKGAERIL VISKCLFDDEAEYACEAKTARTSGLL TVIEEEAVFTKNLHDLEVNENETVRL ICEISKPNAEVTWFKGDEEVPDGGRF EYISDGRKRILVIRNAHPEDAGKYTC KLPSSSTTGKLTVHELAAEFLTRPQN LEVLEGDKAEFACSVSKEAITVQWL WGDTVLEPGDKYDIISDGKKRTLVV KDSVVGDAGKYTVMVGEAKATARL TVIEKTQDYNSLKDQEVNEGQEIIFN CEVNKEGAKEKWYKNGEAIFDSAK YIIVQKDLVYTLRIRDTQLKDQATYS ISLSNHRGEHAESSAALTVLEEGLRIV EPLEDIETMEKKTVTFWCKVNRLNA TLKWTKNGEEITFNKRILYKIDKYKH SLIIKDCGFKDEGEYTVTAGQDKSVA ELLITEAPADFIEHLQDQTVTEFDDA VFTCQLSKEKASVKWYRNGREIKEG KKYQFEKDGNLHRLIIKDCRLDDECE YSCGVDDRKSRARLFVEEIPVEIIRPP QDVYEAPGADVIFMAELNKDGVEV KWLRNNMIIIQGDKHQMMSEGKVH RLQVCEIKPRDQGEYRFIAKDKEARA KLELAAAPRIKTGDQNLVVDVGNPL TMTVPYDAYPRAEAEWLKGEESLPT TTVDTTTDCTTFKIYEAKKSDKGRY KVVLRNKHAQAEAFINVEVIDVPGP VRNLEVTEIYDGEVGLAWQEPESDG GSKIIGYVVERRDIKRKTWIVVTDHA ENCEYTVTGLQKGGVEYLFRVSARN RVGTGEPVETERPVEAKSKFEVPGPP QNVEVTDVNRFGRTLTWEAPEYDG GSPITGYVIELRNRASIKWEPTMTTG ADELSAVLTDVVENEEYFFRVRAQN MVGVGKPSHPTRAVKITDPIERPSGN INLDHSDQTKTSVQLTWEPPLEDGGS PILGYIIERKEEGTDKWIRCNPKLVPA CAFKVTGLKAGSSYYYRVSAENAAG VSDPAEAIGPLTADDPFVDLQWPLSA FKDGLEVIVPEPIKIRVPITGYPIPTAT WSFGDQVLEEGGRVTMKTTSTFAEL VITPSERPDKGIYTLTLENPVSSVSGEI DVNVLARPSAPKELKVVDVSRSTVQ LSWEPPEDDGGSPVIDYIIEKREVSRK TWIKVMDHVIDQEFSVPDLIQGKEYL FRVCACNKCGPGEPAYIDEPINMSAP ATVPDPPENVKWRNPTSKGIFLTWEP PKYDGGARIKGYLVEKCQRGTDKW EICGEPVIETKMEVTKLKEGEWYAY RVKALNRIGASKPSKPTDDIQAIDAK EAPEIFLDVKLLAGLTVKAGTKIELP AKITGKPEPQITWTKAEKLLRPDDRI TIETTPNHSTVTITDSKRSDSGTYIIEA VNSSGRATAVVEVNVLDKPGPPAAF DVSEITNESCLLTWNPPRDDGGSKIT NYVLEKRATDSEIWHKLSSTI SEQ ID A6BLM7 Titin isoform EGEEEWEETYDKREVAYEEEQIIHEE NO: 58 (UniProtKB) Ch12 VVEVPKKPVPERKPPAITAEKKEKKE (Fragment)/Gallus VTVHKVVKKPVDEKVEITTQRVAEE gallus KLKQAEVTKKIPSAKPTPMIIEEKVM KKEAHKEVEEEEEREVISEELETHHV EVPDVPKKIPEEKVPITVPKKPEPTPA KEPEVHKIIEEKIKVSEPKKPEVPPAK VPEVPKKVVKEEVSVEVPKKPEPPPA KVPEVPKKVVPEEKVHVEVPKKAEP PPPKVLRKKPEEEEPKPEKEPKPEVK PVPTPVEKIKKPEVPEVPKKKTEIPVI KKEEKHVPEPPKEPKPAAISVPGPEP KPAVALKSPKPAAEPAPAITTAPVTT PWGKKAEAKPTKEETPKGISPVKA KKTPSPADAERRKLRPGSGGEKPPEE PPFTYQLKAVPLKFVKEMKDIVLKE AESVGSSAIFEVLISPSTAITSWMKDG SNIRESPKHKFIADGKDRKLHIIDVQL SDAGEYTCVLRLGNKEKTSTAKLIVE ELPVRFVKTLEEEVTVVKGQPLYLTC ELNKERSVVWRRDGKIIK SEQ ID A6BLM8 Titin isoform SDKYKIKIEGDRHILKIKNCQLEDQG NO: 59 (UniProtKB) b11 NYRIVCGPHIASARLTVIAEPIQFTKSI (Fragment)/Gallus QNIVVSEHQSATFECEVSFDDAVVT gallus WYKGPTELTESPKYSFRSEGRCHYM TIHNVTAEDEGVYSVIARLEPRGEAR STAELYLVTKEIKLELKPPDVPDAKV AVPPQKPAEAAPIPILLPLIPTPEEKKP AEKKVPVKKVSKKVVKKGPKEIPPP EVPEILPEKPKEVKITSMARREEIHEE KMEIYEKPKKVYEEWEEDYGEDHD YYFKEEGYDEGEEEWEETYDKREVA YEEEQIIHEEVVEVPKKPVPERKPPAI TAEKKEKKEVTVHKVPDVPKKIPEE KVPITVPKKPEPTPAKEPEVHKIIEEKI KVSEPKKPEVPPAKVPEVPKKVVKE EVSVEVPKKPEPPPAKVPEVPKKVVP EEKVHVEVPKKAEPPPPKVLRKKPEE EEPKPEKEPKPEVKPVPTPVEKIKKPE VPEVPKKKTEIPVIKKEEKHVPEPPKE PKPAAISVPGPEPKPAVALKSPKPAA EPAPAITTAPVTTPVVGKKAEAKPTK EETPKGISPVKAKKTPSPADAERRKL RPGSGGEKPPEEPPFTYQLKAVPLKF VKEMKDIVLKEAESVGSSAIFEVLISP STAITSWMKDGSNIRESPKHKFIADG KDRKLHIIDVQLSDAGEYTCVLRLG NKEKTSTAKLIVEELPVRFVKTLEEE VTVVKGQPLYLTCELNKERSVVWRR DGKIIKDKPGKFALGIIGLSHSLTITDS DDSDAGTYTVTVEDSELSCSSCVKV VEVIRDWLVKPIRDQHVKPKGTATF TCDIVKDTPNIKWFKGDEEIPAEPTD KTEILKEGNKIFLKIKNAGPADIGEYS VEVEGRRYPAKLTLGEREVELLKPLE DVTVYEKETANFDTEISEEDIPGEWK LKGEILRPSPTCEIKAEGGKRFLTLHK VKLEQAGEVLYQALNAVTTAILTVK EIELDFAVPLKDVTVPEKRQARFEC SEQ ID P79757 Connectin/titin KSRLRRRREREITEITSEEEEEIEMVQ NO: 60 (UniProtKB) (Fragment)/Gallus HVHREFSPPSRLLRRRRSLSPTYIELM gallus RPVSELIRPRSRPPEESERRSPTPERTR PRSPSPVSTERSLSRFERMARFDIFSR YESMKSALKTQKTMERKYEVLTQQP FTLDHAPRITLRMRSHRVPCGHNTRF ILNVQSKPTADVKWYHNGIELQESS KIHFSNTSGVLTLEILDCHIDDSGTYR AVCTNYKGECSDYATLDVTGGDYT TYSSQRRDEEVPRSILPDLTRTEAYA VSSFKKATAAEASSSVREVKSEVSAT RESLLSYEHHASSEEKITASEEKSLEE RTVHKAFKSTLPATILTKPRSITVSEG ETARFSCDVDGEPAPTITWVRAGQPI VSSRRFQITRTQYKSTFEISLVQIADE GSYTVVVENSEGRQEAHFTLTVQRK RIPEKAITSPPRIKSPEPRVKSPEPVKS PKRVKSPEPISTPSKAKSPPGDKTAPV EKVQLPTASPPKIKEQLKAETLGDKV KLSCAVESSVLSIREVAWYKDGKKL KEDHHFKFHYAADGTYELKIHNLTE SDKGEYTCEIMGEGGISKTNFQFTGQ VFKNIHSQVQSVSETPKSVEKGDKVL AVSTQKKSSAATEEKAAIEEVIKKSI VTEDVKQLQAEIRASSTQMTVSEGQ KVTLKANIPGASEVKWVLNGMELR NSDDYRYGISGSNHTLTIKKASNKDE GILTCEGKTDEGTIKCQYVLTFSKEPS NEPAFITQPKSQNVNEGQDVLFTCEV SGDPSPEVEWLRNNQPIAVSSHMRA TRSKNTYSLEIRNAAVSDTGKYTVK AKNYHGQCSATASLTVFPLIEEPPKE VVLKTSGDASMHESFSSQSFQMAAS KQEASFSSFSSSSMTETKFASMSAKS MSSMKESFVEMSSSSIMGKSSMAQL ESSTSKMLKSGVRGVPPKIEALPSDIS IDEGKVLTLSCAFSGEPAPEITWYCR GRKITSQDQQGRFHIETSEDLTTLIIM DVQKNDGGIYTLNLGNEFGTDSATV NINIRS SEQ ID Q98918 Connectin/titin MTTKAPTFTQPLQSVVALEGSAATFE NO: 61 (UniProtKB) (Fragment)/Gallus AHISGFPVPEVSWYRDGQVLSAATLP gallus GVQISFSDGRAKLVIPSVTEANSGRY TIQATNGSGQATSTAELLVTAGTAPP NFSQRLQSMTARQGSQVRLDVRVTG IPTPVVKFYRDGVEIQSSPDFQILQEG DLYSLIIAEAYPEDSGTYSVNATNNV GRATSTAELLIQGEEEAVPAKKTKTI VSTAQISQTRQARIEKKIETHFDARSL TSVEMVIEGAAAQQLPHKAPPRMPP RPTSKSPTPPVITAKAQMARQQSPSP VRQSPSPVRHVRAPTPSPVRSVSPAG RISTSPIRPVKSPSPIRKAQVVTPGAE VLPPWRQEGYSATAEAQMKETRVST SATEIRTEERWEGRYGLQEQVTISGA AAGEVAAGAKEVRKEPEKTPVPTVII ATDK AKEQERISTAREEISARHEQVHVSHE QIEAGKRAEAVATVVAAVDQARVR SPWETEQVDETYVKKKTLEYGYKEH AVKDHEAQAEHHVATKEVKTVYVP PEKHIPAAEKKEVHVSTEIKRETEAKI EKTIHIEHPRPRTASPHFTVSKIAVPK PDHTYEVSIAGSAMATLEKELSATSA AQKITKPVKPPQLKPHEVKIKPESAPP QFPFTEAAETYKAHYDVETKKEVDV SIKGEAVREDHLLLRKESEAKVTETA RVPVPAEIPVTPPTLVWGLKNKTVTE GESVTLECHISGHPQPTVTWYREDY KIESSMDFQITFKAGLARLVIREAFAE DSGRFTCTATNKAGSVSTSCHLHVK VSEETETRETISEKVVTEEKSYVETK DVVMEDVSAAAEEVSGEPVPPFFIRK PVVHKLIE GGSIIFECQVGGNPKPHVLWKKGGV PLTTGYRYKVSYKRETGECKLEISMT FADDAGEYTIVIRNKFGEASATVSLL EEADYEAYIKSQQEMMYQTQVTAY VQEPKVAEVAPPISYGDFDKEYEKE QALIRKKMAKDTVMVRTFVEDEEFH ISSFEERLIKEIELRIIKTTLDELLEEDG EEMMIDISESEAIGAGFDLRLKNYRT FEGTGVTFHCKTTGYPLPKIAWYKD GKRIRHGERYHMEVLQDGSASLRLP VVLPEDEGIYTVFASNMKGNAICSA KLYVEPVAPTATPGYMPGPEVMRRY RSISPRSPSRSPARSSPSCSPARRLDET DEGQLERLYKPVFVLKPTSVKCSQG QTARFDLKVVGRPMPETYWFHNGQ QVVNDYTHKIVIKEDGTQSLIIVPAM PEDSGEWAVIA QNRAGKASVSVTLSVEAKEDLVRPR FVERLRNVSVKEGSRLHMAVKATG NPNPDIVWLKNSDIIVPHKYPRIRIEG TKGAAALNIESTARQDAAWYTATAI NKAGRDTTRCKVNVEVEHAEPEPER RLIIPKGTYKAKEIAAPELEPLHLRYG QEQWEEGDLYDKEKQQKPFFKKKL TSLRLKQFGPAHFECRLTPIGDPTMV VEWLHDGKPLEAANRLRMINEFGYC SLDYGVAYSRDSGVITCRATNKYGT DHTSATLIVKDEKSLVEESQLPEGRR GMQRIEELERMAHEGALPAVAVDQ KEKQKPELVLVPEPARVLEGETARFR CRVTGYPLPKVNWYLNSQLIRKSKR FRLRYDGIHYLDIVDCKSYDTGEVK VTAENPEGFIEHKVKLEIQQREDFRS VLRRAPEPRHEPVVT EPGKLLFEVQKIDKPAEATTKEVVKL KRAERITHEKLSEESEELRSKFKRRTE EGYYEAITAVELKSRKKDESYEEML KKTKEELLHWTKEIPEEEKKALPPEG KITIPTFKPEKVELSPSMEAPKIFERIQ SQTVAQGTDAHFRVRVVGKPDPECQ WFRNGVQIERTDRIYWYWPEDNVCE LVIRDVTADDSASIMVKAVNIAGETS SHAFLLVQAKQLISFIQNLQDVVAKE RDSMATFECETSEPFIKVKWFKNGIEI HSGEKYRMHSDRKAHFLSVLAVEM SDADDYSCALVEDESVKTTAKLIVE GAVVEFIKELEDVEVPESFTGELECE VSPEDIEGKWYHGDVELSSNHKYVL ASRRGRRILTIKDVNKDDQGEYSFVV DGKRTHCKLKMKPRPMTILQGLTDQ KVCEGDIV QLEVKVSVENVEGVWMKDGHEIQS SDRIHIVLDKQSHMLLIEDATQEDSG TYSFSIPGLELSTTGQVTVYSVEIIVPL KDVHVVEGTKAILECKVSAPDVTSS KWYLNDHQIKPDERVQAVCKGTKQ RLVITRTHASDEGHYKLMVGKVETS CNVTVEEIEIIRGLHDITCTETQNVSF EVELSHSGIDVIWHFKGQEIKAGPKY KIEARGKIYKLTVVKMMKDDEGEY VFYAGGKKTSGKLIVAGGAISKPLA DLTVAESQRAVFECEVANPESEGQW LKNGKPLPMTDQYRAETDGVKRRL NIPAAKMDDMGEYSYEIASSKTSAK LHVEAVKIKKTLKNLTVTETQEAVFS VELSHPDVKGALWIKNGVELESNDK YEISVKGTVHTLKIKHCVVTDESVYS FKLGKIGANARLHVE TVKIIKKPKDVTALENAVVSFELSVS HDTVPVRWFHKNVELKQSDKYKMI SQRKVHKLMLHNISPADAGEYTAFV GQLECKAKLFVETIHITKTMKSIEIPE TKTASFQCEVSHFNVPSVWLKNGVE IEMSEKFKIVVQGKLHQLNIMNTSSE DSAEYTFVCGNDRVSATLTVKPILIT SMLEDINAEEKDTITFEVTVNYEGIS YKWLKNGVEIKSTDKCQIRTKKLTH SLSIRNVHFGDAAEYSFVAGKAASSA TLYVEARHIEFRKHIKDIKVVEKKRA IFECEISEPDVQVQWMKDGQELQIGD RMKIQREKYVHRLIIPSTKMSDAGQY TVVAGGNTSSANLIVEGRDVRIRSIR KEIQVIERQRAEIEFEVNEDDIEPQWY KDGIEINFHYEERYSYVVERRIHRMSI FETTYS DAGEYTFVAGRNRSSVVLYVNAPEP PQIIQELQPTTVESGKPARFCAIISGKP QPKVSWYKDDQQLSPGFKCKFLHD AQEYTLLLIETFPEDSAVYTCEAKND YGVATTSASLSVEIPEVVSPELEVPV YPPAVIVPLRDAVTSEGQSARFQCRV TGTDLKVSWYSKDREIKPSRFFRMT QFEDTYQLEIAEAYPEDEGTYTFVAS NSVGQVTSTAILKLEAPEKIMYEKLE EEIEMEVKVAPILRRRLEPLEVAVNH VAKFTCEVETTPNVKFQWYKAGREI YDGDKYSIRSSNYLSTLEIPRPQVVD CGEYSCKASNQHGSVSSTAFLTVTEP PRFIKKLDSSRLVKQHDSTRYECKVG GSPEIKVTWYKGETEIHPSEKYSMSF VDSVAVLEMHNLSVEDSGDYSCEAQ NPAGSAST STSLKVKAPPAFTKKPHPVQTLKGSD VHLECELQGTPPFQISWYKDKREIRS SKKYKVMSENYLASIHILNVDTADV GEYHCKAVNDVGSDSCIGSVTLRAP PTFVKKLSDVTVVVGETIELQAAVE GAQPISVLWLKDKGEIIRESENLWIS YSENVASLKIGNAEPTNAGKYICQIK NDAGFQECFAKLTVLEPAVIVEKPGP VKVTAGDSCTLECTVDGTPELTARW FKDGNELSTDHKYKISFFNKVSGLKI LNAGLEDSGEYTFEVKNSVGKSSCT ASLQVSDRIMPPSFTRKLKETYGQLG SSAVLECKVYGSPPILVSWFHDGQEI TSGDKYQATLTDNTCSLKVNGLQES DMGTYSCTATNVAGSDECSAFLSVR EPPSFVKKPEPFNVLSGENITFTSIVK GSPPLEVKWF RGSIELAPGHKCNITLQDSVAELELF DVQPLQSGDYTCQVSNEAGKISCTT HLFVKEPAKFVMKVNDLSVEKGKN LILECTYTGTPPISVTWKKNGVILKHS EKCSITTTETSAILEIPNSKLEDQGQY SCHIENDSGQDNCHGAITILEPPYFVT PLEPVQVTVGDSASLQCQVAGTPEM IVSWYKGDTKLRGTATVKMHFKNQ VATLVFSQVDSDDSGEYICKVENTV GEATSSSLLTVQERKLPPSFTRKLRD VHETVGLPVTFDCGIAGSEPIEVSWF KDNVRVKEDYNVHTSFIDNVAILQIL KTDKSLMGQYTCTASNAIGTASSSG KLVLTEGKTPPFFDTPITPVDGIIGES ADFECHISGTQPIRVTWAKDNQ SEQ ID Q07784 Titin RESDHRAWTPVSYTVTRQNAVVQG NO: 62 (UniProtKB) (Fragment)/Gallus LTEGKAYFFRIAAENIIGMGPFTETTK gallus ELIIRDPITVPDRPEDLEVKAVTKNSV TLTWNPPKYNGGSDITMYVLESRLIG KEKFHRVTKEKMLDRKYTVEGLKE GDTYEYRVSACNIVGQGKPSFCTKPI TCKDEIAPPTLDLDFRDKLIVRVGEA FSLTGRYSGKPTPKITWLRDDIVLKE DDRTKIKTTPTTLCLGILKSVREDSG KYCVTVENSTGSRKGFCQVTVVDRP SPPVGPVIFDEVHKDHMIVSWKPPLD DGGSEITNYIIEKKDTHRDLWMPVTS ATVKTTCKIPKLLEGREYQVRIYAEN LYGISDPLISDEMKAKDRFSVPDAPE QPVIKDVTKDSAVVVWNKPYDGGK PITNYIVEKKETMATRWVRVTKDPIF PSTQFKVPDLLEGCQYEFRVSAENEI GVGNPSPPSKPIFARDPIVKPSPPVNP EAVDKTKNSVDLTWQPPRHDGNGKI IGYLVEYQKVGDEEWKKANLTPDSC PETKYKVTGLTEGLTYKFRVMAVNA AGESEPAYVPDPVEVKDRLEPPELIL DANMAREQHVRAGDTLRLSAVIKG VPFPKVSWKKEDKEVSPKADIEVTG VGSKLEIRNTVHEDGGIYSLTVENPA GSKTVSVKVLVLDKPGPPRNLQVSD VRSDSCYLTWKEPEDDGGSVITNYV VERKDVASAQWVSVSSSSKKRSHM AKYLMEGTQYLFRVAAENLFGRGPY VETLKPIKAMDPLHPPGPPKNLHHID VDKTEVSLVWNRPDRDGGAEITGYL VEYQEDGADEWTKFQTVPMLDCVV TGL SEQ ID Q90720 Titin VTTKQTVISRTREGIVTSQEQRHISRE NO: 63 (UniProtKB) (Fragment)/Gallus KVRKEPEKTPVPTVIIVTAKVKEQERI gallus STAREEISARHEQVHVSHEQIRREDV KPSVPKVVITTDKPKAPVLISQSKEGI ATKKEHVSISHEKIKKEAKKTTAVLK IIAPVTVSRTREEITAKPEQMHLAYD QIEAGKRAEAVATVVAAVDQARVR SPWETEQVDETYVKKKTLEYGYKEH AVKAHEAQAEHHVATKEVKTVYVP PEKHIPAAEKKEVHVSTEIKRETEAKI EKTNHIEHPRPRTASPHFTVSKIAVPK PDHTYEVSIAGSAMATLEKELSATSA EQKITKPVKPPQLKPHEVKIKPESAPP PFPFTEAAETYKAHYDVETKKEVDV SIKGGAVREDHLLLRKESEAKVTETA RVPVPAEIPVTPTHLVWGLKNKTVT EGESVTLECHISGHPQPTVTWYRDD YKIESSMDFQITFKAGLARLVIREAF AEASGRFTCTATNKAGSVSTSCHLH VKVSEETETRETISEKVVTEEKSYVE TQDVVMEDASPPPEEVSGEPVPPFFI RKPVVHTLIVGGSIIFEFHVGGNPKPH VLLKKGGVPLTTGYRYKVSYKRETG SEQ ID F1N757 Titin/Bos taurus MATQAPTFTQPLQSVVVLEGSTATFE NO: 64 (UniProtKB) AHISGFPVPEVSWFRDGQVISTSTLPG VQISFSDGRAKLTIPAVTKANSGRYS LRATNGSGQATSTAELLVTAETAPPN FTQRLQSMTVRQGSQVRLQVRVTGI PTPVVKFYRDGAEIQSSLDFQISQEGE LYSLLIAEAYPEDSGTYSVNATNSVG RATSTAELLVQGEEVVPAKRTKTIVS TAQISETRQTRIEKKIEAHFDARSIAA VEMVIDGAAGQQLPHKTPPRIPPKPK SRSPTPPSIAAKAQLARQQSPSPIRHS PSPVRHVRAPTPSPVRSVSPAGRISTS PIRSVKSPLLVRKAQTPTMPPGPEVPP PWKQEGYVASSEAEMRETTVTSATQ IRTEERWEGRYGIQEQVTISGAAGAA ASTTFAAGAVAAGAAEVKQEADKS AAVATVVAAVDMARVREPVISAVE QTAQRTTTTAVHIQPAHEQIRKEAEK TAVTKVVVAADKAKEQEVKARTRE VITTKQEQVHVTHEQIRKETEKAFVP KVVISAAKAKEKETKITGEITTKQEQ KQITQETIIQKAETAAVSMVVVETAK PTTLETILGAQEEIVTQQDQMHITHE KILKETRKTVVPKVIVATPKVKEQDV VSRTREGITTKREQVQVTHEKMRKE AEKTALSTIAVATAKAKEQETILRTR EEMATRQEQIQVTHGKVGVGKKAE AVATVVAAVDQARVREPREPRHVEE SYAQQTTLEYGYKEHISATKVAEQPP RPASEPQVVPKAVKPGVIHAPSETHI ATTDQMGMHISSQIKKTTDLTSERLV HVDKRPRTASPHFTVSKISVPKTEHG YEASIAGSAIATLQKELSATPSAQKV TKSVKAPTVKPAEARVRAEPTPSPQF PFAETPETFKSQAGIEVKKEVGVSITG VAVREERFEALRERETKVTETARVP APVEIPVTPPTLVSGLKNVTVIEGESV TLECHISGYPSPKVTWYREDYQIESSI DFQITFQSGIARLLIREAFAEDSGRFT CTAVNEAGTVSTSCYLAVQVSEEFE KETTAVAEKITTEEKRFVESRDVVM TDTSITEEHAGPGEPAAPFFITKPVVQ KLVEGGSIVFECQVGGNPKPHVYWK KSGVPLTSGYRYKVSYNKQTGECRL VISMTFADDAGEYTIVIRNKHGETSA SASLLEEADYESLMKSQQEMLYQTQ VTAFVQEPKVGEIAPGYVYSEYEKE YEKEQALIRKKMAKDTVMVRTFVE DQEFHISSFEERLIKEIEYRIIKTTLEEL LEEDGEEKMAVDISESEAIEAGFDLR VKNYRILEGMGVTFHCKMSGYPLPK IAWYKDGKRIKHGERYHMDFLQDG RASLRIPVVLPEDEGIYTAFASNMKG NAICSGKLYVEPAAPLSAPTYIPTPEP VSRIRSISPRSVSRSPIRMSPARMSPA RMSPGRRLEETDESQLERLYKPVFVL KPTSFKCVEGQTARFDLKVVGRPMP ETFWFHDGQQIVNDYTHKVVVKED GTQSLLIVPATPSDSGEWTVVAQNR AGKSSISVILTVEAVEHQVKPVFVEK LRNLNIKEGSRLEMKVRATGNPNPDI VWLKNSEIIVPHKYPKIRIEGTKGEA ALKIDSTVSQDSAWYTATAINKAGR DTTRCKVNVEVEFAEPEPERRLIIPRG TYRAKEIAAPELEPLHLRYGQEQWE EGDLYDKEKQQKPFFKKKLTSLRLK RFGPAHFECRLTPIGDPTMVVEWLH DGKPLEAANRLRMINEFGYCSLDYA VAYSRDSGVITCRATNKYGTDHTSA TLIVKDEKSLVEESQLPEGRKGLQRI EELERMAHEGALTGVTTDQKEKQKP DIVLFPEPVRVLEGETARFRCRVTGY PQPKVNWYLNGQLIRKSKRFRVRYD GIHYLDIVDCKSYDTGEVKVTAENPE GVIDHKVKLEIQQREDFRSVLRRAPE PKPEFHVHEPGKLQFEVQKVDRPVD TTETKEIVRLKRAERITHEKVSEESEE LRSKFKRRTEEGYYEAITAVELKSRK KDESYEELLRKTKEELLHWTKELTE EEKKALAEEGKITIPTFKPDKIELSPS MEAPKIFERIQSQTVGQGSDAHFRVR VVGKPDPECEWYKNGVKIERSDRIY WYWPEDNVCELVIRDVTAEDSASIM VKAINIAGETSSHAFLLVQAKQLITFT QELQDVVAKEKDTMATFECETSEPF VKVKWYKDGVEIHTGDKYRMHSDR KVHFLSVLMIDTSDAEDYSCVLVED ENVKTTAKLIVEGAVVEFVKELQDIE VPESFSGELECIITPENIEGKWYHKGV ELKSNGKYTITSRRGRQNLTVKDVT KEDQGEYSFVVDGKKTTCKLKMKP RPIAILQGLSDQKVCEGDIVQLEVKV SLENVEGVWMKDGEEVQPGDRVHI VIDKQSHMLLIEDMTKEDAGHYSFTI PSLGLSTHGRVSVYSVDVITPLKDVN VLEGTKAVLECKVSVPDVTSVKWYL NDEQIKPDDRVHAIVKGTKQRLVINR THASDEGPYKLVVGRVETSCDLSVE KIKIIRGLRDLTCTETQNVVLEVELSH SGIDVLWNFKDKEIKPSSKYKIEAHG KIYKLTVLNMMKDDEGEYTFYAGE NRTSGKLTVAGGAISKPLTDQTVAES QEAVFECEVANPDSEGEWLKDGKHL PLSKTIRSESDGRKRRLIIAATKLDDI GEYTYKVATSKTSAKLKVEAVKIKK TLKNLTVTETQDAVFTVELTHPNVK GVQWIRNGVVLESSDKYTVSVKGTV YSLRIKNCAVADESVYGFKLGKLGA SARLHVETVKIIKKPKDVTALENATV SFEVSVSHDTVPVKWFHKNVEIKPSD KHRLVSERKVHKLMLQHISPSDAGE YTAVVGQLECKAKLFVETLHITKTM KNIEVPETKTASFECEVSHFNVPSMW LKNGVEIEMSEKFKIVVQGKLHQLII MNTSTEDSAEYTFVCGNDQVSATLK VTPIMITSMLKDINAEEKDTITFEVTV NYEGISYKWLKNGVEIKSTDRCQMR TKKLTHSLNIRNVHFGDAAEYTFVA GKATSTATLYVEARHIEFRKHIKDIK VLEKKRAMFECEVSEPDITVQWMKD GQELQLVDRIKIQKEKYVHRLLIPST RMSDAGKYTVVAGGNMSSANLFVE GRDVRIRSIKREVQVIEKQRAVVEFE VNEDDVDAHWYKDGIEINFQVQERH QYVVERRIHRMFISETRHSDAGEYTF VAGRNRSSVTLYVNAPEPPQILQELQ PITVQSGKPARFCAVISGRPQPKISWY KEEQLLSTGFKCKFLHDGQEYTLLLI EAFPEDAAVYTCEARNDYGVATTSA SLSVEVPEVVSPDQEMPVYPPAIVSP LQDAVTSEGRPAHFQCRVSGTDLKV SWYSKDKKIKPSRFFKMTQFEDTYQ LEVAEAFPEDEGIYTFVASNAVGQVS STATLRLEAPEAMLHEQIEMEMKEFS ISLLSGKEERPQTSSWDLQLFDINETL EPLSEPSVYSPKFDSKKEGNAPVFIKE VSNAEISIGDVAKLFVTVTGIPTPQIQ WFFNGAMLTPSADYKFVFDGNDHSL IILFTKLEDEGEYTCIASNEYGQAMC SAYLKINSEAEGHEEPESAEKSLEKL QGPCPPYFLKELKPVHCVQGLPAIFE YVVLGEPAPTVLWFKENKQLCTNVY YTIIHNPDGSGTFIVNDPQKEDGGLY VCKAENVWGGSTCAAELFVLLEDTD MTDATCKAAPTPEAPEGFPHTSVKD PAVETFDSEQEVVTFVKDAILKASLI AEEKQQFSYEHVVKTNELSSQVTLK AEQLQSTVILEHDMPTPESTRELLSIS GTIHVQPIKELPPSLQLQIAQSQDTLP REDTLMCQEPESQVVLSDTEKIFPSF MSIEEISLSTVESLQTLLAEPEESYPQP LIKETPAHSYPTSVAEEVLLSKDKTV SAVDRGQRETSQKQESQNALFLNQN LAEGHAQSLQGPDVTVSRVSSEHTC TESEILMESVDQLDSAGQDFAARIEE GQSLRFPLACEEKQVLLKEEHSDTLA LPLNQTTEYKKEPMAVNGVQEVQGS DFLSKESLLPGIPEEQRLNLKTQIRSA LQAAVASEQLSLFSEWLRNIEQVEV KAIDFTQEPNCIMCTYLITSVKSLTEE VTVTIKGIDPQMADLKTELKEALCSI CEEMNILTAEEPGIEKGATVVLQEDV SFSSSQKLEAITEPEAESKYLVSKEEIS YFKVESQVKAVGTTTASAAVSDEKQ DELPKPSEEKEKVSEGGTEEVGTVEI QEAEDEEDRKLGEDGPDVQTPLVDT IAEEGDIISLTSCITNAKEVNWYFESK LVPSDEKFKCLQDQNTYTLVIDKVN REEHQGEYTCEALNDNGKATTSAKL TVVKRAAPVIRRRIEPLEVALGHLAK FTCEIHSAPNVRFQWFKAGREIYESD KCSIRSANYVTTLEILRTQVVDCGEY TCKASNEYGSVSCTATLTVTEAYPPT FFSRPKSVTTFVGKAAKFLCTVTGTP VIETIWQKDGMALSPSPTCKISDVDN KHILELSNLTVHDKGVYSCKASNKF GADTCQAELDIIDKPHFIKELEPVQSA INKKIHLEGQVDEDRKVTITWSKNG QKLPPGKDYKIYFEDKIASLEIPLAKL KDSGTYVCTASNEAGSSSTSATVTIR EPPSFVKKVDPSYLMLPGESARLHCR LKGSPVIQVTWFKNNKELTESNTIRM SFVNSEAVLDITDVKVEDSGTYSCEA VNDVGSDSCSAEIVVKEPPSFIKTLEP ADIVRGTNALLQCEIAGTGPFEISWF KDKKQIRSSKKYRLFSQKSTVSLEIFS FNSADVGDYECVVANEVGKCGCVA THLLKVEPPTFVKKVDDFTALAGQT VTLQAAVRGSEPISVTWMKGQEIIKE DGKIKMSFANGVAVLIIPDVQISFGD KYTCLAENEAGSQTSVGELIVKEPAK IIERAELIQVTAGDPATLEYTVAGTPE LKPKWYKDGRPLVASKKYRISFKNN VAQLKFYSAELHDSGQYTFEISNEVG SSSCETTFTVLDRDIAPFFTKPLRNVD SVVSGTCRLDCKIAGSLPMRVSWFK DGKEVTSSDRYRIAFVEGTASLEISR VDMSDAGNFTCRATNSVGSKDCSG ALIVQEPPSFVTKPASKDILPGSAVFL KSTFQGSTPFTIRWFKGDKELVSGGN CYINKEALESSLELYSVKTTDSGTYT CKVSNVAGAVECSANLFVKEPATFV ERLELSQLLKKGDTTQLACKVTGTPP IKITWFANDREIKESSKHKMSFVEST AVLRLTDIAVEDSGEYMCEAQNEAG SDHCSSILIVKESPYFTKEFKPIEVLKE YDVMLLAEVAGTPPFEITWFKDNTT LRSGRKYKTFIQDRLVSLQILKFVAA DVGKYQCRVTNEVGSSTCSARVTLR EPPTFVKKIESTSSLRGGTAAFQATL KGSLPITVTWLKDNEEITEDDNIRMT FENNVASLYLSGIEVKHDGKYVCQA KNDAGIQRCFAVLSVKEPATIIEEAV SIDVTQGDPATLQVKFSGTKEITAKW FKDGQELTLGQKYKISVTDTVSILKII STEKRDSGEYTFEVQNDVGRSSCKA SINVLDLIIPPSFTKKLKKMDSIKGSSI DLECIVAGSHPISIQWFKDDQEITASE KYKFSFHDNTAFLEISQLEGSDSGTY TCSATNKAGHNQCSGHLTVKEPPYF LEKPQSQDVNPNTRVQLKALVGGTA PMTIKWFKDNKELHSGAARSVWKD DTSTILELFSAKAADSGTYICQLSND VGTATTKASLFVKEPPQFIKKPSPVL VLRNGQSTTFECQITGTPEIRVSWYL DGNEITAVEKHGISFIDGLATFQISGA RVENSGTYVCEAQNDAGTASCSIEL KVKEPPTFIRELKPVEVVKDSDVELE CEVMGTSPFQVTWLRNNKEIRSSKK YTLTDRVSVFNLNINRCDPSDTGDY QCIVSNEGGSCSCSARVSLKEPPSFIK KIENITTVLKSSATFQSTVAGSPPISIT WLKDDQILDEDDNVHISFVNNVATL QIRSVDNGHSGRYTCQAKNESGVER CYAFLLVQEPAQIIEKAKSVDVTERD PVTLECVVAGTPELKVKWLKDGKQI VPSRYFSMSFENNVASFRIQSVMKQ DSGEYTFKVENDFGSSSCDAYLRVL DQNIPPSFTKKLTKMDKVLGSSIHME CKVSGSLPISAQWFKDGKEISTSAKY RLVCHENTVSLDVNNLELEDTANYT CRVSNVAGDAACSGILTVKEPPSFLV KPERQQAIPDSTVEFKAVLKGTPPFKI KWFKDDVELASGPKCFIGLEGSTSFL NLYSVDASKTGQYTCQVTNDVGSDS CTTTLLVTEPPKFVKKLEATKIVKAG DSARLECKITGSPDIRVVWYRNEHEL PASDKYRMAFIDSVAVLQMNYLGTE DTGDFICEAQNPAGSTSCSTKVIVKE PPVFSSFPPVVETLKNAEVSIECELSG TPPFEVVWYKDKRQLRSSKKYKIAS KNFHASIHILNVDTLDIGEYHCKAQN EVGSDTCICIVKLKEPPRFVSKLNSLT VVAGEPAELQASIEGTQPISVQWLKE KEEVVRESENIRITFVENVATLQFSK AEPANAGKYICQIKNDGGMRENMA TLSFISEPAVIVEKAGSMTVTVGETC TLECKVAGTPELSVEWYKDGKLLTS SQKHKFSFYNKISSLKILSVEKQDAG TYTFQVQNNVGKSSCTAVVDVSDR MVPPSFTRRLKDTIGVLGTSCILECK VAGSSPISVAWFHGKTKIVSGAKYQ TTFSDNVCTLQLNSLDSSDMGSYTC VAANVAGSDECRAVLAVQEPPSFVK EPEPLEVLPGKNITFTSVIRGTPPFKV GWFRGARELVKGDRCNIYFEDTVAE LELFNVDISQSGEYTCVVSNNAGQTS CTTRLFVKEPAVFVKKLSDHSVEPG KSIILESTYKGTLPISVTWKKDGFNIT PSEKCSIVTTEKTCILEILNSTKRDAG RYSCEIENEAGRDVCEALVSTLEPPY FVTELEPLEASVGDSVSLQCQVAGSP EITVSWYKGDTKLRPTPEYRTYFTNN VATLVFNKVNINDSGEYTCKAENSIG TASSKTVFRIQERQLPPSFARQLKDIE QTVGLPVTLTCRLNGSAPIQVSWYR DGVLLRDDENLQTSFVDNVATLKIL QTDLSHSGQYSCSASNPLGTASSSAR LTAREPKKSPFFDIKPVSIDIIAGESAD FECHVTGAQPMRITWSKDNKEIRPG GNYTITCVGNTPHLRILKVGKGDSG QYTCQATNDVGKDMCSAQLSVKEP PKFVKKLEASKVAKQGESIHLECKIS GSPEIKVSWFRNDSELHESWKYNMS FVDSVAVLTINEASAEDSGDYICEAH NGVGDASCSTALTIKAPPVFTQKPSPI GALKGSDVVFQCEISGTPPFEVVWV KDRKQVRSSKKFKITSKNFDASLHIL NLEAADVGEYYCKATNEVGSDTCV CTLKFKVEPPRFVKKLSDTSTLVGDA VELRAVVEGFQPISVVWLKDKGDVI RESENTRISFVDNVATLQLGSPEASD SGKYVCQIKNDAGMRECSAILTVLEP ARIIEKPEPMTVTTGNAFALECVVTG TPELSAKWFKDGREIAADSKHHITFV NKVASLKIPCAEMSDKGLYSFEVKN SVGKSTCTVSVHVSDRIVAPSFIRKL KDINAIVGASVVLECRVSGSAPISVG WFQDGNEIVSGPKCQSSFSENVCTLN LSFLEPSDTGTYTCVAANVAGSDECS AVLTIQEPPSFEQTPDSVEVLPGTSLT FTSVIRGTPPFKVKWFKGSRELVSGE SCSISLEDFVTELELFEVEPLQSGDYS CLVTNDAGSASCTAHLFVKEPATFV KRLADFSVETGSPIVLEATFTGTPPIS VSWMKNEFALTQSQNCSITMTEKSTI LEILDSTIEDYAQYSCLIENEAGQDIC DAVVSVLEPPYFIEPLEHVEAVIGEPI TLQCKVDGTPEIRISWYKEHTKLRSA PAYKMQFKNNVASLVINKVDHSDV GEYTCKAENIVGAVASSSVLVIKERK LPPSFARKLKDVQETLGFPVAFECRI NGSEPLQVSWYKDGVLLKDDANLQ TSFVHNVATLQILQTDQSHVGQYNC SASNPLGTASSSAKLVLLEHEVPPFF DLKPVSVDLALGESGSFKCHVTGTA PMKITWAKDNREIRPGGNYKMTLVE NTATLTVLKIGKGDAGQYTCYASNV AGKDSCSAHLGVQASIEPPRFIKKLE PSRIVKQDESTRYECKIGGSPEIKVL WYKGETEIQESSKFRMSFVDSVAVL EMHGLSVEDSGDYTCEACNAAGSAS STTSLKVKALEPPIFRKKPHPVETLK GADVHLECELQGTPPFQVSWHKDKR ELRSGKKYKIMSENFLTSIHILSVDAA DVGEYQCKATNDVGSDTCVGSITLK APPRFVKKPSDVSTIVGEEVQLQATV EGAEPISVVWFKDKGEIVRESDNIWI SYSENTATLQFSRAETANAGKYTCQI KNDAGMQECSATVSILEPAAIVEKPE SITVTTGDTCTLECSVTGTPELTTKW FKDGKELTSDNKYKISFFNKVSGLKII NVAPSDSGVYSFEVQNPVGKDSCTA SVQVSDSDRIVPPSFTRRLKETNGLS GSSVVMECKVYGSPPISVSWFHERN EISSGRKYQTTLTDNTCALTVNMLEE SDAGNYTCIATNVAGSDECSAPLTV REPPSFVQKPDPMDVLTGTNVTFTSI VKGTPPFSVSWFKGSTELVPGDTCN VSLEDSVAELELFDVDTSQSGEYTCI VTNEAGKVSCTTHLYVKAPARFVKK LNDYSIEKGKPLILEGTYTGTPPISVT WKKNGINVTPSQRCNITTTERSAILEI PSSTVEDAGQYNCYIENTSGKDSCSA QILILEPPYFVRQLEPVKVTVGDSASL QCQLGGTPEIAVSWFKGDTKLRPTA TYKMHFRNNIATLVFNQVDSNDSGE YICRAENSVGEVSSSTFLTVQEQKLP PSFSRQLRDVQETVGLPVVFECAVSG SEPISVSWFKDGRPVKDSPNIQASFL DNVATLNIFQTDRSFAGQYSCTATNP IGSASSSARLILTEGKNPPFFDIPLAPV DAVVGESADFECHVTGTQPIKVAWA KDNREIRSGGNYQISYLENSAHLTIL KVDKGDSGQYTCYAVNEVGKDSCT AQLNIKERLIPPSFTKKLSETVEETEG NSFKLEGRVAGSQPISVAWYKNNIEI HPTSNCEITFKNNTLLLQIKRAGMDD AGLYTCKVSNDAGSALCTSSIVIKEP KKPPVFDQHLTPVTASEGEFVQLSCH VWGSEPIRIQWLKAGREIKPSDRCSF SFANGTAVLELKDVTKADAGDYVC KASNVAGSDTSKSKVTIKDKPAAVP AAKKAAVDGKLFFVSEPQSIRVVEK TTATFIAKVGGDPIPNVKWTKGKWR QLNQGGRILIHQKGDEAKLEIRDTTK TDSGLYRCVAFNKHGEIESNVNLQV DERKKQEKIEGDLRAMLKKTPVLKK GAGEEEEIDIMELLKNVDPKEYEKY ARMYGITDFRGLLQAFELLKQTEGE ETHRLEIEEIEKSEKDEKEFEELVSFIQ QRLSQTEPVTLIKDIENQTVLKDNDA VFEIDIKINYPEIKLSWYKGTEKLEPS DKFEISIDGDRHTLRVKNCQLKDQG NYRLVCGPHIASAKLTVIATEPAWER HLQDVTLKEGQTCTMTCQFSVPNVR SEWFRNGRILKPQGRYKTEVEHKVH KLTIADVRAEDQGRYTCKHEDLETS AELRIEAEPIQFTKRIQNIVVSEHQSA TFECEVSFDDAIVTWYKGPTELTESQ KYNFRNDGRCHYMTIHNVTPDDEG VYSVIARLEPRGEARSTAELYLTTKEI KLEMKPPDIPDSRVPIPTMPIRAIPPEE IPPTAVPPIPLLLPLPEEKKPPEKRVEV TKKAVKKDAKKVVAKPKEEAPPPK VIEVPKKPPRPTALIPAEAPEIIDVSSK AEEVKITTITRKKEVQKEKEAVYERK QAVYEEKKVYIESLEEPYDELEVETY TEPYEEPYYEEPEEDYEETQVEAKRE VHEEWEEDFEEGQEYYEREEGYDEG EEEWEETYQEREVVQVQKEVYEESR ERKIPAKVPEKKELPPPKVVKKPVVE KIEKTSRRMEEEKVQVTKVPEVSKKI VPQKPSRTPVQEEIIEVKVPAVHTKK MVISEEKMFFAAHTEEEEEVSVTVPE VQKKTVTEEKVHVAVSKKIEPPPKV PEPPKKPEEVVPVPVPKKVEPPPAKV PEVPKKPVPEEKKPVPVPKKEPAAPP KVPEVPKKPVPEEKVPVPIAKKKEAP PAKVPEVQKRVVTEEKITIVTEREESP PPAVPEIPKKKLPEEKRPVPRKEEEVP PPKVPAVPKKPVPEEKVPVPVPVAK KAPPPRAEVSKKMVMEEKRFAAEEK LSVTVPQRVEVMRHEVSAEKEWRYS EEEERVSVSVYREEEREEEEVEVTEY EVMEEPEEYIVEEEEHFISEEVEAEPA EESFQVPEKKIIPKPKIPAKVEEPPPA KVPEAPKKIVPEKKIPAAVPKKEKVP PTKVPEEPKKPVPEKRAPPKVAKIEE PPPTKVTERHMQITQEEKVHVAVTK KVEPPRPKVPEEPKRAVPEEKFPKLK PRKEEEPPAKVTELRKRAVKEEKVSI EVAKREPPAAKEVTVTAEKEWAYT KEEEAVSVEREEEYEEYEEYDYKEFE EYEPTEEYDQYEEYEEREFEHYEEYK EHEEYVTEPEKPVPVKPIQEEPVPTKP KAPPAKVPKKVIPEEKVPVPIPKKLK PPPPKAREEAKKVVEEKIQISVIKREK EQVTEPAAKVPMKPKRVVTEEKVPV PKKEVAAPVRVPEVPKKEEPEEIAFE EEVVTHEEEYYEEEEEEEEYIHEEEEI ITEEEVVPVKVFKVPEVPKKPVPEEK KPVPVPKKKEAPPAKVPEIPKKPEEK VPVPVPKKEKAPPAKVPEVPKKPVPE EKVPVPEEKVPVPVPKKVEAPPAKV PEVPKKPVPEKKVPVPAPKKVEAPPA KVPEVPKKVLPEEKKPTPIRKKVEPP PPKVPKKREPVPVPIPAALLREEKVV HKEEIVVEEEEVLPEEEEISPEEEVPL EEEEEEEEVLPEEEEVPPEEEEVPPEE EEVPPEEEEFVPEEEVVPEKEEVLPEI KPKVPVPARVPEVKKKVPEKKVIIPK KEEVPPAKVPEVPKKVEEKRIIVPEEE EVPPVEVFEEPEEPIPEEEIPEEAPIVE EVEEEAPPRVPEVVKKAVPEAPTPVP KKVEVPPAKVPKKVPEEKPPVPVQK KEAPPAKVPEVPKKVPEKKVPVPKK EVVPPAKGRAVLEEKVSVAFHKEEV VEERTELEIVEAQEEALEEEFHEVEE YFEEEEFHEVEEFLKVEKYRAEEEVH RAEEVHRVIEVLEAEEEEAYEKPKAP PKGPEISEKVIPPKKPPTKVVPRKEPP AKVPEVPKKIVVEEKVHVPEEPKVA PAKVPEAPKEVVPEKKVPAAPPKKP EVPPVTVPEAPKEVVPEKKVPVVPPK KPEVPPAKVPEVPKAAVPEKKVPEAI PPKPESPPPAVPEAPKEVVPEKKVPV MPPKKPEVPPAKVPEAPKEVVPEKK VPVMPPKKPEVPPAKVPEVPKAAVP EKKVPEAIPPKPESPPPAVYEEPEEIA PEEPPVEVVEEPEPVPAPPPKVTVPPK KPVPEKKPPAVVAKKPEPPPAKVPEV PKEVVPEKKVPPVVPKKPEVPPPKVP EVPKEVVPEKKVAVPKKPEVPPAKV PEVPKKPVLEEKPAIPIPEKVESPPPEE EEEPVPVVEEEEPVPVVEEEEPVPVV EEVEPEAPPPAVPEEPKKIVPEKKVP VIKKPEPPPPKEPEPEKKVIEKPKLKP RPPAPPPAPPKEDVKEKIFQLKAVSK KKVPEKPAVPEKVELTPLKVAGGEK KVRKLLPEPKPQPKEEVILKSVLRKR PEEEEPKVEPKKVEKIKKPAVPEPPP KAVEEVEAPPAVTKKERKIPEPTKVP EIKPPIPLPAPEPKPKPEAEVKIIKPPP VEPPPTPIAAPVTVPVVGKKAEAKAP KEEAAKPKGPIKGVAKKTPSPIEAER KKLRPGSGGEKPPDEAPFTYQLKAV PLKFVKEIKDIVLTEAESVGSSAIFEC LVSPSTAVTSWMKDGSNIRESPKHRF IADGKDRKLHIIDVQLSDAGEYTCVL RLGNKEKTSTAKLIVEELPVRFVKTL EEEVTVVKGQPLYLSCELNKERDVV WRKDGKIVVEKPGKIVPGVIGLLRAL TINDADDSDAGTYTVTVENANNLEC SSCVKVVEVIRDWLVKPIRDQHVKP KGTAVFTCVIAKDTPNIKWYKGYDE IPWEPTDKTEIIRDGNHIHLKVKNAM PEDIDEYAVEIEGKRYPAKLTLGERE VELLKPIEDVTIYEKESASFDAEISEV DVPGQWKLKGELLRPSPTCEIKAED GKRFLTLHNVKLDQAGEVLYQALN AVTTAILTVKEIELDFAVPLKDVTVP EKRQARFECVLTREANVIWSKGPDII KSSDKFDIITDGKKHILVINNSQFDDE GVYTAEVEGKKTSARLFVTGIRLKFI SPLEDQTVKEGETATFVCELSHEKM HVVWFKNDAKLHTSRTVLISSEGKT HKLEMREVTMDDISQIKAQVKDLSS TANLKVIEADPYFTVKLHDKTGVEK DEIVLRCEVSKDVPVKWFKDGEELL PSPKHSIKADGLRRILKIKKADLKDK GEYVCDCGTDTTKANVTVEARLIKV EKPLYGVEVFVGETARFEIELSEPDV HGQWKLKGEPLTASPDCEIIEDGKK HILVLYNCRLDMTGKVSFQAANAKS AANLKVKELPLIFITPLSDVKVFEKD EAKFECEVSREPKTYRWLKGTQEITG DDRIELIKDGTKHSLVIKSAAFEDEA KYIFEAEDQHTSGKLIIEGIRLKFLTPL KDVTAKERESAVFTVELSHDNIRVR WFKNDQRLHTSKFVSMDDEGKTHSI TFRNLSIDDTSQIKVEAMGLSSEAKL TVLEGDPYFTGKLQDYTGVEKEEVIL QCEISKADAPVKWFKDGQEIKPSKN AVIKADGKKRMLILKKPLKSDIGQYT CDCGTDKTSGKLNIEDREIKLVRPLY SVEVMETETARFETEISEDDIHANWK LKGEALLQTPECEIKEEGKTHFLILH NCRLDQTGGVDFQAANVKSSAHLR VKPRVIGLLRPLKDITVTAGETATFD CELSYEDIPVEWYLRGKKLEPSDKV VMRSEGRVHTLTLRDVKLEDAGEV QLVAKDFKTQAKLFVKEPPVEFTKP LEDQTVEEEATAVLECEVSRENAKV KWFKNGTEILKSKKYEIVADGRVRK LIIHGCTPEDIKTYTCDAKDFKTSCNL NVVPPHVEFLRPLTDLRVKEKEMAR FECEISRENAKVHWFKDGAEIKKGK KYDIISKGAVRILVINKCLLDDEAEYS CEVRTARTSGMLTVLEEEAVFTKNL ANIEVSETDTVKLVCEVSKPGAEVT WYKGDEEIIETGRYEILTDGRKRILII QNAHLEDAGNYNCRLPSSRTDGKVK VHELAAEFISKPQNLEILEGEKAEFV CSISKENFEVQWKRDDKTLESGDKY DVIADGKKRVLVVKDATLQDMGTY VVMVGAARAAAHLTVIEKLRIIVPLK DTRVKEQQEVVFNCEVNTEGAKAK WFRNDEAIFDSSKYIILQKDLVYTLRI RNAQLDDQANYNVSLTNHRGENVK SAANLIVEEEDLRIIEPLKDIETMEKK SVTFWCKVNRLNVTLKWTKNGEEV AFDNRVLYRIDKYKHSLIIKDCGFPD EGEYVVTAGQDKSVAELLIIEAPTEF VEHLEDQTVTEFDDAVFSCQLSREK ANVKWYRNGREIKEGKKYKFEKDG SIHRLIIKDCRLDDECEYACGVDDRK SRARLFVEEIPVEIIRPPQDILEAPGAD VVFLAELNKDKVEVQWLKNNMIIVQ GDKHQMMSEGKIHRLQICDIKPRDQ GEYRFIAKDKEARAKLELAAAPKIKT ADQDLVVDVGQPLTMVVPYDAFPK AEAEWFKENEPLSSKTVDTTAEQTSF RILKAKKEDKGRYKVVLQNKHGKA EGFINLKVIDVPGPVRNLEVTETFDG EVSLAWEEPLTDGGSKIIGYVVERRD IKRKTWVLATDRADSCEFTVTGLQK GGVEYLFRVSARNRVGTGEPVETDS PVEARSKYDVPGPPLNVTVTDVNRF GVSLTWEPPEYDGGAEITNYVIELRD KTSIRWETAMTVRAEDLSATVTDVV EGQEYSFRVRAQNRIGVGKPSAATPF IKVADPIERPSPPVNLNASDQTQSSV QLTWEPPLKDGGSPILGYIIERCEEGK DNWIRCNKKLVPELTYKVTGLQKGN KYLYRVSAENEAGVSDPSEILGPLTA DDADAEPTMDLSAFKDGLEVIVPNPI KILVPSTGYPRPTATWSFGDKVLEAG DRVKMKTLSAYAELVISPSERPDKGI YTLKLENRAKAISGEIDVNVIARPSA PRELKFGDITKDSVHLTWEPPEDDGG SPLTGYVIEKREVSRKTWTKVIDSVT DLEFTVPDLLQGKEYLFKVCACNKC GPGEPAYVDEPVNMSAPATVPDPPE NVKWRDRTAKSIFLTWDPPKHDGGS RIKGYIVEKCPRGSDRWVACGEPVA DTKMEVTGLEEGQWYAYRVKALNR LGASKPSKPTEEIQAIDTQEAPEIFLD VKLLAGITVKAGTKIELPATVTGKPE PKITWTKADMLLKQDKRITIENVPKK STVTIMDSKRSDTGRYIIEAVNVCGR ATAVVEVNVLDKPGPPAAFDITDVT NESCLLTWNPPRDDGGSKITNYVVE RRATDSDMWHKLSSTVKDTKFKAT KLTPNKEYIFRVAAENMYGVGEPVQ ATPITAKFQFDPPGPPTRLEPSDITKD AVTLTWCEPDDDGGSPITGYWVERL DPESDKWVRCNKMPIKDTTYRVKGL TNKKKYRFRVLAENLAGPGKPSKST EPILIKDAIDPPWPPGKPVVKDIGRTS LMLNWTKPEHDGGAKIDSYVIEMLK TGTEDWVRVAEGVPTTQHLLPGLME GQEYSFRVRAVNKAGESEPSEPSDPV LCREKLYPPSPPRWLEVINITKNTAD LKWTVPEKDGGSPITNYIVEKRDVR RKGWQTVDTTVKDTKCTVTPLTEGS LYVFRVAAENAIGQSDYCEVEDSVL AKDTFTTPGPPYALAVVDVTKRHVD LKWEPPKNDGGRPIQRYVIEKKEKL GTRWVKAGKTSGPDCHFRVTDVIEG TEVQFQVRAENEAGVGHPSEPTEILK IEDPTSPPSPPLDLHVTDAGRKHIAIA WKPPEKNGGSPIIGYHVEMCPVGTE KWMRVNSRPIKDLKFKVEEGVVPDK EYVLRVRAVNAVGVSEPSEISENVV AKDPDCRPTIDLETHDIVVIEGEKLSI PVPFRAVPVPTVSWHKDGKEVKASD RLTMKNDHISAHLEVPKSVRADAGI YTVTLENKLGSATASINVKVIGLPGP CRDLKASDVTKSSCKLTWEPPEYDG GSPILHYVLERREAGRRTYIPVMSGE NKLSWTVKDLIPNGEYFFRVKAVNK IGGGEYIELKNPVIAQDPKQPPDPPV DVEVHNPTAEAMTITWKPPLYDGGS KIMGYIIEKIAKGEERWKRCNEHLVP VLTYTAKGLEEGKEYQFRVRAENAA GISEPSRATPPTKAVDPIDAPKVIMKT SLEVRRGDEIALDAIISGSPYPTITWL KDESIITPEEIKKRVEPLVRRRRGEVQ EEQPFVLPLTQRLSIDNSQKGESQLR VRDSVRPDHGLFMIKAENDHGIAKA PCTVCVLDIPGPPINFVFEDMRKTSV LCKWEPPLDDGGSEILNYTLEKKDK TKPDSEWMVVTSTLRHCKYSVTKLI EGKEYLFRVRAENRFGPGPPCVSKPF MAKDPFEPPDAPDKPIVEDVTSNSML VTWNEPKDNGSPILGYWLEKREVNS THWSRVNRNLLNSLKTKVDGLLEGL TYVFRVCAENAAGPGKFSPPSDPKT AQDPISPPGPPVPRVTDTSSTTIELAW EPPAFNGGGEIVGYYVYKQLVGTNE WSRCTEKMIKPREYTVREIREGADY KLRVTAVNVAGEGPPGETEPVTVAE PQAEPPTVELDVSVKGGIQIMAGKTL RIPAVVTGRPVPTKVWTIEEGELDKD RVEIENVGTKSELIIKNALRKDHGRY VITATNSCGSKFAAARVEVFDVPGPV LDLKPVVTNRKMCLLNWSDPEDDG GSEITGFIIERKDAKMHTWRQPIETER SKCDITGLLEGQEYMFRVIAKNKFGC GPPVEIGPILAVDPLGPPTSPERLTYT ERTKSTITLDWKEPRSNGGCPIQGYII EKRRHDKPDFERVNKHLCPTTSFLVE DLDEHQMYEFRVKAVNEIGESEPSLP LNVVIQDDEVPPTIKLRLSVRGDTIK VKAGEPVNIPADVTGLPMPKIEWSK NETVIEKPTDALKITKEEVSRSEAKTE LSIPKATREDKGTYTVTASNRLGSVF RNVHVEVYDRPSPPRNLAVTDIKAES CYLTWDAPLDNGGSEITHYIIDKRDA SRKRAEWEEVTNSAVERRYGIWKLI PNGQYEFRVRAVNKYGISDECKSDK VVIQDPYRTPGPPGKPKVLERTKGS MLVSWTPPLDNGGSPITGYWLEKRE EGGAYWSRVSRAPITKVGLKGVEFN VPRLIEGVKYQFRAMAINAAGVGPP SEPSDPEVAGDPIYPPGAPSRPEVKD KTKSSITEAWKPPAKDGGSPIKGYIV EMQEEGTTDWKSVNEPDKLLPTCEC VVPNLKELKKYRFRVKAVNEAGESE PSDTTGEIPATDIQEVPEVFIDIGAQD CLICQAGTQIRIPAVIKGRPTPKSSWE FDGKAKKAMKDGVHDIPEDAQLET AENSSVIIIPECKRSHSGKYSITAKNK AGQKTANCRVKVMDVPGPPKDLKV SDITRGSCRLSWKMPDDDGGDRIKG YVIEKRTIDGKAWTKVNPNCVSTSF VVPDLIAGQEYFFRVRAENRFGVGA PAETIQRTTARDPIYPPDPPIKLKIGLV TKNTVHLSWKPPKNDGGSPVTHYIV ECLAWDPTGTKKEAWRQCNKRDVE ELEFTVEDLIEGGEYEFRVKAVNAA GVSKPSATVGPVIVKDQTCPPAIELK EFMEVEEGTDVNIVAKIKGVPFPTLT WFKAPPKKPDNKEPIVYDTHVNKLV VDDTCTLVIPQSRRSDTALYTITAVN NLGTASKEMRLNVLGRPGPPVGPIKF ESISADQMTLSWLPPIDDGGSKITNY VIEKREANRKTWVRVSSEPKECTYTI PKLLEGHEYVFRIMAQNKYGIGEPLD SEPETARNLFSVPGAPDKPTVSSVTR NSMTVNWEEPEYDGGSPVTGYWLE MKDTTTKRWKRVNRDPIKAMTLGV SYKVTGLIEGSDYQFRVYAINAAGV GPASLPSDPATARDPIAPPGPPFPKVT DWTKSSADLEWSPPLKDGGSRVTGY IVEYKEEGKEEWEKAKDKEVRGTKL VVTGLKEGAFYKFRVRAVNIAGIGEP GEVTDAIEMKDRLELPDLQLDASVR DRIVVHAGGVIRIIAYVSGKPPPTVT WNMNERALPQEATIETTAISSSMVIK NCQRSHQGVYSLLAKNAAGERKKTI IVDVLDVPGPVGIPFLAHNLTNDSCK LTWFSPEDDGGSPITNYVIEKREADH RAWTPVTYTVTRHNATVQGLIQGKA YFFRIAAENSIGMGPFVETTDALVIR DPITVPERPEDLEVKEVTKESVTLTW NPPKYDGGSDIINYVLESRLIGTEKFH KVTSDNLMSRKYTVKGLKEGDTYE YRVSAVNIVGQGKPSFCTKPITCKDE LAPPTLDLDFRDKLTIRVGEAFALTG RYSGKPKPKVSWFKDEADVLEDDRT HIKTTPTTLALEKLKAKRSDSGKYSV VVENSTGSRKGVCQVNVVDRPGPPV GPVIFDEVTKDYMVISWKPPLDDGG SEITNYIIEKKEVGKDVWMPVTSASA KTTCKVSKLLEGKDYIFRIHAENLYG ISDPLVSDSMKAKDRFRVPDAPDQPI VTEVTKDSALVSWNPPHDGGKPITN YILEKRETMSKIWARVTKEPIHPYTK FRVPDLLEGCHYEFRVSAENEIGIGD PSPPSKPVFAKDPIAKPSPPVNPEAID TTCNSVDLTWQPPRRDGGSKILGYIV EYQKVGDEEWKRANHTPESCPETNY KVTGLRDGQSYKFRVIAVNAAGESD PAHVPEPVLVKDRLEPPELILDANMA REQHIRVGDTLRLSAIIKGVPFPKVT WKKEDRAAPTKARIDVTPVGSKLEI RNAAHEDGGIYSLTVENPAGSKTVS VKVLVLDKPGPPRDLEVSEIRKDSCY LTWKEPLDDGGSVITNYVVERKDVA STEWSPLSTTSKKKSHLAKHLNEGN QYVFRVAAENQYGRGPFVETPKPIK AVDPLHPPGPPKNLHHVDVDKTEVS LVWNKPDRDGGSPITGYLVEYQEEG TPDWIKFKTVTNLACVVTGLQQGKT YRFRVKAENIVGLGLPDTTIPIECQEK LVPPSVELDVKLIEGLVVKAGTTVRF PAIIRGVPIPTAKWTTDGNEIKTDEHY TVETDNFSSVLTIKNCLRKDTGEYQI TVSNAAGTKTVAVHLTVLDVPGPPT GPINILEVTPEYMTISWLPPKDDGGSP VINYIVEKKDTKKDTWGVVSSGSSK TKLKVPHLQKGYEYVFRVKAENKIG IGPPLDSVPTVAKHKFSPPSPPGKPVV TDITENAATVAWTLPKSDGGSPITGY YLERREVTGKWVRVNKTPLVDLKFR VTGLYEGNTYEFRVFAENLAGLSGP SPSSDPIKACRPIKPPGPPINPKLKDKT RESADLVWTKPLSDGGSPILGYVVE CQKAGTTQWDRINKDELIRQCAFRV PGLIEGNEYRFRIKAANIVGEGEPREL AESVIAKDILHPPEVELDVTCRDVITV RVGQTIRILARVKGRPEPDITWSKEG KALVRDKRVNIIHELPRVELQIKEAV RADHGKYIISAKNSSGHAQGFAIVNV LDRPGPCQNLKVTNVTKENCTISWE NPLDNGGSEITNFIVEYRKPNQKGWS IVASDVTKRLIKANLLANNEYYFRVC AENKVGVGPTIETKTPILAINPIDRPG EPENLHIADKGKTFVYLKWRRPDYD GGSPNLSYHVERRLKGAADWERVH KGSIKETHYMVDKCVENQIYEFRVQ TKNEGGESDWVKTEEVVVKEDLQK PVLDLKLSGVLTVKAGDTIRLEAGV RGKPFPEVSWTKDKDATDLTRSPRV KIDTSGDSSKFSLTKAKRSDGGKYV VTATNTAGSFVAFATVNVLDKPGPIR NLKITDVCSDRCSLRWDPPEDDGGC EIQNYILEKCESKRMVWSTFSSTILTP GTTVTRLIEGNEYIFRVRAENKIGTGP PTETKPVIAKTKYDKPGRPDPPEVTK VSKEEMTVVWAPPEYDGGKSITGYY LEKKEKHSTRWVPVNKSAIPERRLK VQNLLPGHEYQFRVKAENEVGIGEP SLPSRPVVAKDPIEPPGPPTNLKVVD TTKSSITLGWGKPVYDGGAPIIGYVV EMRPKKADMSPDEGWKRCNAAAQL VRMEFTVTSLDENQEYEFRVCAQNQ VGIGRPAELKDAIKPKEILEPPEIDLD ASMRKLVTVRAGCPIRLFAIVRGRPA PKVTWRKVGIDNVVRKGQVDLVDT MAFLVIPNSTRDDSGKYSLTLVNPAG EKAVFVNVRVLDTPGPVSDLKVSDV TKTSCHISWAPPENDGGSQVTHYIVE KREAERKTWATVTPEVKKTSFHVTN LVPGTEYFFRVTAVNEYGPGVPTDV PKPVLATDPLSEPDPPRKLEVTEMTK NSAVLAWLPPLRDGGAKIDGYIVSY REEEQPADRWTEYSVVKDLSLVVTG LKEGKKYKFRVAARNAVGVSLPREA EGVYEAKEQLLPPKILMPEQITIKAG KKLRIEAHVYGKPNPVCKWKKGED DVVTSSHLAVHKAENSSVLIIKDVTR KDSGYYSLTAENSSGTDTQKIKVIVM DAPGPPQPPFDISDIDADACSLSWHIP LEDGGSNITNYIVEKCDVSRGDWVT ALASVTKTSCRVGKLIPGQEYIFRVR AENRFGISEPLTSPKMVAKFPFGVPS EPKNARVTKVNKDCIFVAWDRPDSD GGSPITGYLIERKERNSLLWVKANDT PVRSTEYPCAGLVEGLEYSFRIYALN KAGSSPPSKPTEYVTARTPVDPPGKP EVIDVSKSTVSLVWARPKHDGGSKII GYFVEACKLPGDQWIRCNTSPHQIPQ EEFTVTGLEENAQYQFRAIAKTAVNI SQPSEPSDPVTIMAESVPPRIELSVAM KSLLTVKAGTNVCLDATVFGKPKPT VSWKKDGTLLKPSEGIKMAMKRNL CTLELFSVTRKDSGDYTITAENPSGS KSATIKLKVLDKPGPPASVKINKMYS DRAMLSWEPPLEDGGSEITNYIVDKR ETSRPNWAQVSATVPITSCSVEKLIE GHEYQFRICAENKYGVGDPILTEPAI AKNPYDPPGRCDPPVISNVTKDHMT VSWKPPADDGGSPITGYLVEKRETH AVNWTKVNRKPVIERTIKATGLQEG TEYEFRVIAINKAGPGKPSDASKAVY AQDPLYPPGPPAFPKVYDTTRSSVSL TWGKPAYDGGSPIIGYLVEVKRADS DNWVRCNLPQKLQKTRFEVTGLME NTEYQFRVYAVNKIGYSDPSDVPDK HCPKDILIPPEGELDADLRKTLILRAG VTMRLYVPVKGRPPPKITWSKPNVN LRERVGLDIKSTDFDTFLRCEKVNKY DAGKYILTLENSCGKKEYTIVVKVL DTPGPPVNVTVKEISKDSAYITWDPPI IDGGSPIINYVVEKRDAERKSWSTVT TECSKTSFRVSNLEEGKSYFFRVFAE NEYGIGDPGETRDAVKASETPGPVV DLKVTSVTKSSCSIGWKKPRSDGGSR IIGYVVDFLTEENKWQRVMKSLSLQ YTTKDLTEGKEYTFRVSAENENGEG TPSEITAVAKDDVVAPDLDLKDLPDL CYLAKENSNFRLKIPIKGKPVPSVSW KKGEDPLATDTRVSVESSAVNTTLV VYDCRKSDAGKYTITLKNVAGTKEG TLTIKVVGKPGIPTGPIKFEEVTAEAV TLKWGPPKDDGGSEITNYILEKRDSV NNKWVTCASAVQKTTFRVMRLHEG MEYTFRVSAENKYGVGEGLKSEPIV ARHPFDVPDAPPPPNIVDVRHDSVSL TWTDPRKTGGSPITGYHIEFKERNSL LWKRANKTPIRMKDFKVTGLTEGLE YEFRVMAINLAGVGKPSLPSEPVVAL DPIDPPGKPEVISVTRNSVTLVWTEP KYDGGHKLTGYIVEKRELPSKSWTK ANHVNVPDCAFTVTDLVEGGKYEFR VRAKNTAGAISAPSESTDTIICKDEYE APTIVLDPTIKDGLTVKAGDTIILSAIS ILGKPLPKSSWSKAGKDIRPSDIVQIT STPTSSMIAVKYATRKDAGEYTITAT NPFGTKSEHVKVTVLDVPGPPGPIEIS NVSAEKATLTWTPPLEDGGSPIKSYV LEKRETSRLLWTVVAEDIQSCRHVA TKLIQGNEYVFRVSAVNQYGKGEPV QSEPVKMVDRFGPPGPPGKPEVSNV TKNTATVSWKRPVDDGGSEITGYHV ERREKKSLRWVRATKTPVSDLRCKV TGLQEGNTYEFRVSAENKAGIGPPSD ASNPVLMKDVAYPPGPPSNARVTDT TKKSASLAWGKPHYDGGLEITGYVV EHQKVGDEGWIKDTTGTALRITEFV VPDLETKEKYNFRISAINDAGVGEPA VIPNVEIVEREMAPDFELDAELRRTL VVRAGLSIRIFVPIKGRPAPEVTWTK DNINLKNRANIENTESFTLLIIPECNR YDTGKFVMTIENPAGKKSGFVNVRV LDTPGPVLNLRPTDITKESVTLHWDL PLIDGGSRITNYIVEKREATRKSYSTV TTKCHKCTYKVTGLSEGCEYFFRVM AENEYGIGEPAETTEPVRASEAPSPP DSLNIMDITKSTVSLAWPKPKHDGGS KITGYVIEAQRKGSDQWTHITTVKGL ECVVKNLTEGEEYTFQVMAVNSAG RSAPRESRPVIVKEQTMLPELDLRGI YQKLVIAKAGDNIKVEIPVLGRPKPT VTWKKGDQILKQTQRVNFENTATST ILNINECVRSDSGPYPLTARNIVGEVG DVITIQVHDIPGPPTGPIKFDEVSSDF VTFSWEPPENDGGVPISNYVVEMRQ TDSTTWVELATTVIRTTYKATRLTTG VEYQFRVKAQNRYGVGPSITSAPVV ANYPFKVPGPPGTPQVAAVTKDSITI SWHEPLSDGGSPILGYHVERKERNGI LWQTVSKALVPGNIFKSSGLTDGIAY EFRVIAENMAGKGKPSKPSEPILALD PIDPPGKPVPLNITRHTVTLKWAKPE YTGGFKITSYIVEKRDLPNGRWLKA NFSNILENEFTVSGLTEDAAYEFRVIA KNAAGAISPPSEPSDAITCRDDIEAPR IMVDVKFKDTITLKAGEAFKLEADV SGRPPPTMEWTKDGKELENTAKLEI KIADFSTNLVNKDSLRRDGGAYTLT ATNPGGFAKHIFHVKVLDRPGPPEGP LAVSEVTSEKCVLSWLPPLDDGGAKI EYYVVQKRETSRLAWTNVASEVQV TKLKVTKLLKSNEYIFRVMAVNKYG VGEPLDSEPVLAVDPYGPPDPPKNPE VTSITKDSMVVCWGHPDSDGGSEIIN YIVERRDKAGQRWVKCNKKTLTDL RYKVSGLTEGHEYEFRVMAENRAGI SAPSATSPFYKACDAVFKPGPPGNPR VLDTSRSSISIAWNKPIYDGGSEITGY MVEIALPEEDEWKIVTPPSGLKATSY TITNLTENQEYKIRIYAMNSEGIGEPA LVPGTPKAEDRMLPPEIELDAELRKV VTIRACCTLRLFVPIKGRPAPEVKWT REHGESLDKASIESTSSYTLLIIGNVN RFDSGKYILTIENSSGSKSAFVNVRV LDTPGPPQDLKVKEVTKTSVTLTWE PPLIDGGSKINNYIVEKRESTRKAYST VTTNCHKTSWKVDQLQEGCSYYFR VLAENEYGIGLPAETAESVKASERPL PPGKITLVDVTRNSVSLSWEKPEHDG GSRILGYIVEMQSKGSDKWVTCATV KVTEATITGLIQGEEYSFRVSAQNEK GISDPRQLSVPVIAKDLVIPPAFKLLF TTFTVLAGEDLKVDVPFTGRPTPAVT WHKDDVPLKQTTRVNAESTENNSLL TIKEACREDVGHYIVKLTNSAGEATE TLNVIVLDKPGPPTGPVKMEEVTADS VTFSWGPPKYDGGSSINNYIVEKRDT STTTWQIVSATVARTTIKACRLKTGC EYQFRIAAENRYGKSTYLTSEPVVAQ YPFKVPGPPGTPFVTLSSKDSMEVH WNEPVSDGGSKVIGYHLERKERNSIL WVKLNKTPIPQTKFKTTGLDEGIEYE FRVSAENIVGIGKPSKVSESYVARDP CDPPGRPEPIIVTRNSVTLQWKKPAY DGGSKITGYIVEKKELPDGRWMKAS FTNVIDTQFEVTGLVEDHRYEFRVIA RNAAGVFSEPSESTGAITARDEVEPP QIRMDPKYKDTIVVHAGELFKIDADI HGKPIPTTQWIKGDHELSNTARLEIK STDFATSLSVKDAIRIDSGSYILKAKN VAGEKSVTVNVKVLDRPGPPEGPIVI SGVTAEKCTLAWKPPLQDGGSDIINY IVERRETSRLVWTVVDANVQTLGCK VTKLLEGNEYIFRVMAVNKYGVGEP LESEPVTAKNPFVVPDAPKAPEITAV TKDSMIVVWERPAFDGGSEILGYVL EKRDKEGIRWTRCHKRLIGELRLRVT GLLENHNYEFRVSAENAAGLSEPSPP SAYQKACDPIYKPGPPNNPRVTDITR SSVFLSWGKPIYDGGCEIQGYIVEKC DTSVGEWTMCTPPTGINKTNIEVEKL LEKHEYNFRICAVNKAGVGEHADVP GPVIVEEKLEAPDIDLDLELRKILNIR AGGSLRLFVPIRGRPTPEVKWGKVD GEIRDAAIIDSTSSFTSLVLDNVNRYD SGKYTLTLENSSGTKSAFVTVRVLDT PSPPVNLKVTEITKDSVSITWEPPLLD GGSKIKNYIVEKREATRKSYAAVVT NCHKNSWKIDQLQEGCSYYFRVTAE NEYGIGLPAHTDDPVKVAEVPQPPG KITVDDVTRNSVSLSWTKPEHDGGS KIIQYIVEMQAKHSEKWSECARVKSL EAVITNLTQGEEYLFRVVAVNEKGR SDPRSLAVPIVAKDLVIEPDVRPAFNS YSVQVGQDLKIEVPISGRPKPTITWT KDDLPLKQTTRINVTDSLDLTVLSIK ETHKDDGGHYGITVANVVGQKTASI EIITLDKPDPPKGPVKFDEVSAESITLS WEPPLYTGGCQITNYVVQKRDTTTT VWEWSATVARTTLKVTKLKTGTEY QFRIFAENRYGQSFALESEPIVAQYP YKEPGPPGTPFVTAVSKDSMVVQWH EPINNGGSPVIGYHLEKKERNSILWT KVNKTIIHDTQFKAVNLEEGIEYEFR VYAENIVGIGKASKNSECYVARDPC DPPGTPEAIIVKRHEITLQWTKPAYD GGSVITGYIVEKRDLPEGRWMKASF TNVIETQFTVSGLTEDQRYEFRVIAK NAAGAVSKPSDSTGPITARDEVELPR ISMDPKFRDTIVVNAGETFRLEADVH GKPLPTIEWLRGDKEIEESARYEIKNT DFKALLIVKDAIRIDGGQYILRASNV AGSKSFPVNVKVLDRPGPPEGPVQV TGVTAEKCTLTWSPPLQDGGSDISHY VVEKRETSRLAWTVVASEVVTNSLK VTKLLEGNEYIFRIMAVNKYGAGEP LESAPVLMKNPFVLPGPPKSLEVTNI AKDSMTVCWNRPDSDGGSEIIGYIVE KRDRSGIRWIKCNKRRITDLRLRVTG LTEDHEYEFRVSAENAAGVGEPSPA TLYYKACDPVFKPGPPINAHVVDTT KNSITLAWGKPIYDGGSEILGYVVEI CKADEEEWQIVTPQTGLKATRFEISK LTEHQEYKVRVCALNKVGLGEATA VPGTVKPEDKLEAPELDLDSELRKGI VVRAGGSVRIHIPFKGRPTPEITWSRE EGEFTDKVQIEKAANYTQLSIDNCDR NDAGKYVLKLENSSGSKSAFVTVKV LDTPGPPQNLAVKEVRKDSVLLVWD PPLIDGGAKVKNYVIDKRESTRKAY ANVSNKCSKTSLKVENLTEGAIYYFR VMAENEFGIGVPVETVDAVKASEPP SPPGKVTLTDVSQTSASLMWEKPEH DGGSRILGYVVEMQPKGTEKWSVV AESKVCSAWTGLSSGQEYHFRVKA YNEKGQSDPRVLGVPVIAKDLTIQPS FKLPFNTYSVQAGEDLKIEIPVIGRPR PEISWVKDGEPLKQTTRVNVEKTAT STILHIKESNKDDFGKYTVTATNSAG TATENLSIIILEKPGPPVGPVRFDEVS ADFVVISWEPPAYTGGCQISNYIVEK RDTTTTTWHMVSATVARTTIKITKL KMGSEYQFRIFAENRYGKSAPLDSKP VIVQYPFKEPGPPGTPFVTSVSKDQM LVQWHEPVNDGGSKVIGYHLEQKE KNSILWVKLNKTPIQDTKFKTTGLDE GLEYEFRVSAENIVGIGKASKVSECF VARDPCDPPGRPEAIVITRNNVTLKW KKPAYDGGSKITGYIVEKKDLPDGR WMKASFTNVLDTEFTVSGLVEDQRY EFRVIARNAAGNFSEPSESTGAITAR DEIDAPNASLDPKYKDVIVVHAGETF VLEADIRGKPIPDIVWSKDGRELEET AARMEIKSTIQRTTLVVKDCIRSDGG QYILKLSNVGGTKTIPITVKVLDRPGP PEGPLKVSGVTAEKCYLAWNPPLQD GGASISHYIIEKRETSRLSWTQVSTEV QALNYKVTKLLPGNEYIFRVMAVNK YGTGDPLESEPVIARNPYKPPGPPSPP EVSAITKDSMVVTWARPVDDGGAEI EGYILEKRDKEGVRWTKCNKKTLTD LRFRVTGLTEGHSYEFRVAAENAAG VGEPSEPSVFSRACDALYPPGPPSNP KVTDTSRSSVSLAWNKPIYDGGAPV KGYVVEVKEATADEWTTCTPPTGLQ GKQFTVTELKENTEYNFRICAINSEG VGEPATIPGSVAAKERQEPPEIELDA DLRKVVILRASATLRLFVTIKGRPEPE VKWEKAEGVLTDRAQIEVTSSYTML VIDNVTRFDSGRYNLTLENNSGSKTA FVNVRVLDSPSAPVNLTVREVKKDS VILAWEPPLIDGGAKITNYIVEKRETT RKAYATVTNNCTKNSFKIENLQEGC SYYFRVLASNEYGIGLPAETTEPVKA SEPPLPPGRVTLVDVTRNTATIKWEK PESDGGSKITGYVVEMQTKGSEKWS ICTQVKTLEATISGLTAGEEYIFRVAA INEKGKSDPRQLGVPVIARDIEIKPSV ELPFNTFNVKARDQLKIDIPFKGRPQ ATVSWKKDGQTLKETTRVNVSSSKT VTSLIIKEASREDVGTYELCVSNSAG SVTVPITVIVLDKPGPPGPIHIDEVSC DNITISWNPPEYDGGCQISNYIVEKRE TTSTTWHVVSQAVARTSIKIVRLVTG SEYQFRVCAENRFGKSSYSESSAVVA EYPFSPPGPPGTPKVVHATKSTMLVT WQVPVNDGGSRVLGYHLEYKERSSI LWSKANKTLIADTQMKVSGLDEGL MYEYHVYAENIAGIGKCSKSCEPVP ARDPCDPPGQPEVTNITRKSVSLKWS KPHYDGGAKITGYIIERRELPDGRWL KCNFTNVPETYFEVTELTEDQRYEFR VFARNAADSISEPSESTGPITVKDDVE APRIMMDVKFRDVIVVKAGEVLKIN ADVAGRPLPIISWAKDGVEIEERART EIVSTDYTTLLTVKDCVRRDSGQYV LTVKNVAGTRSMAVNCKVLDKPGP PAGPLEITGLTAEKCSLSWGPPQEDG GAAIDYYIVEKRETSRLAWTICEGEL RTTFCKVTKLLKGNEYIFRVTGVNK YGVGEPLESMAVKALDPFTVPSPPTS LEITSVTKEFMTLCWSRPESDGGSEIS GYIVERREKNSLRWVRVNKKPVYDL RVKSTGLREGCEYEYRVYAENAAGL SLPSESSPLIRAEDPVFLPSPPSKPNV MDSGKTNITIGWVKPLFDGGAPITGY TVEFKKSDETDWQTAIQNLRGTEYT VSGLTTGAEYVFRVRSINKVGASDPS DSSDPQIAKEREEEPVFDLDTEMRKT LIVKAGASFTMTVPFRGRPVPSVSWS KPDTDLRTRAYIDSTESRTSLTIENAN RNDSGKYTLMIQNVLNAASLTLVVK VLDSPGPPANITVHDVTKESAVLSW DVPENDGGAPVKNYHIEKREASKKA WVSVTNNCNRLSYKITNLQEGAIYY FRVSGENEFGVGVPAETKEGVKITEK PSPPEKLGVTSVSRDSVSLAWLKPEH DGGSRIVHYVIEALEKGQTTWMRCA VVKSTHHVVSGLRQNSEYFFRVFAE NQAGLSDPRELLLPVLIKEQLEPPEID MKNFPSHTVYVRAGSNLKVDIPISGK PLPKVTLSRDGVPLKATMRFNTEITA ENLTINLKESVAADAGRYEITAANSS GTTKAFINIVVLDRPGPPTGPVVISDI TEDSVTLKWEPPKYDGGSQVTNYIV LKRETSTAVWTEVSATVARTMIKVM KLTTGEEYQFRIKAENRFGISDHIDSA CVVVKLPYTTPGPPSTPWVTNVTRES ITVGWHEPVSNGGSPVTGYHLEMKD RNSILWQKANKMVIRTTHFKVTTISA GLIYEFRVYAENAAGIGKPSHPSEPV LAIDACEPPRNVRITDISKNSVNLSW QQPAFDGGSKITGYMVERRDLPDGR WAKASFTNVTETHFTVSGLTQNAQY EFRVFARNAVGSISNPSEVVGPITCID SYGGPVIDLPLEYTEVVKYRAGTSV KLRAGISGKPEPTIEWYKDDKELQTN ALVCVENTTDLASILIKDATRLNSGT YELKLRNALGSASATIRLQILDKPGP PGGPIEFKTVTAEKITLLWQPPADDG GAKITHYIVEKRETSRVVWSMVSEN LEECIIKTTKIIKGNEYIFRVRAVNKY GIGDALESHPVIARNAFVTPGPPSVPE VTKINKNSMTVVWSRPVADGGSDIS GYILEKRDKKSLGWFKVLKETIRDTR QKVTGLTEHSDYQYRVCAVNAAGQ GPFSEPSDFYKAADPIDPPGPPAKIRI ADSTKSSITLGWSKPVYDGGSAVTG YVVAMRQGEEEEWTVVSTKGEVRT TEYVVSNLSPGVNYYFQVSAVNCAG QGEPIAMTEPVQAKDILEEPEIDLDV AFRTSIIAKAGEDVHALVPFKGRPPP TVTWRKDEKNLGSDARYSIQNTDSS SILTIPQVTRHDTGKYILTIENGVGQP KSSTVSVKVLDTPAACQNLQVKHVS QGTVTLFWDPPLIDGGSPIINYIIEKK DATKRTWSSVSHKCSSTSFKVTDLSE KTPFFFRVFAVNEIGIGEPCETTEPVK AAEVPAPIRDLSVKDSTKTSVTLSWT KPDFDGGSVITDYIVERKGKGEQTW SHAGISKTCEIEVGKLKELSELEFRVS ARNEKGLSDSVSIGPVTVKELVIPPE VDLSEIPGAQVAVRIGHNVHLELPYK GKPKPSISWLKDGLPLKESELVRLGK TENKLTLSIKNAKKENGGKYTIILDN AVCRNTYPITVITLGPPSKPKGPIRFD EIKADSVIMSWDVPDDDGGGEITCYS IEKREASQTNWKMVCSSVARTTFKV PNLVKDAEYQFRVRAENRYGVSQPL VSNIILAKHQFRIPGPPGKPVTYNVTS DGMSLTWDAPVYDGGSEVTGYHVE KKERNSILWQRINMSPISGREYRATG LMEGLDYQFRVFAENSAGLSSPSDPS KFTLAVSPVDPPGTPDYIDVTRETITL KWNPPLRDGGSKIVAYSIEKRQGSD RWTRCNFTDVSECQYTVTGLSPGDR YEFRIIARNAVGTISPPSQSSGVIMTR DENVAPTVEFGPEYFDGITIKSGESLR IKALVQGRPVPRVTWFKDGVEIEKK MNMEITDVLGSTSLFVRDATRDHRG VYRVEAKNASGSTKAEITVRVQDTP GKPVGPIRFTNITGEKMTLWWDAPH NDGCAPVSHYLIEKRETSRLAWALIE DNCEALSYTATKLITGNEYQFRISAV NKFGVSRPLDSDPVVAQIQYTIPDAP GTPEPSNVTGNSITLTWARPESDGGS EIQQYILERREKKSTRWVKVISKRPIC ETRFKVTGLTEGNEYEFHVMAENAA GVGPASGVSRLIKCREPVNPPGAPTV VKVTDTSKTTVSLEWSKPVFDGGLEI IGYIIEMCKADLGDWHKVNVEACVK TRYTVTDLQAGEEYKFRVSAINAAG KGDSCEVTGTIKAVDRLSAPELDIDA NFKQTHVVRAGASIRLFIAYQGRPTP TAVWSKPDSNLSIRADIHTTDSFSTLT VENCNRTDAGKYTLTVENNSGSKSI TFTVKVLDSPGPPGPITFKDVTRGSA TLMWDAPLLDGGARIHHYVVEKRE ASRRSWQVVSEKCIRQILRVSDLLEG VPYYFRVSAENEYGVGEPYEMPEPM VATEQPAPPRRLDIVDTSKSSVVLAW LKPDHDGGSRITGYLLEMRQKGSDF WVEAGHTKQMTFTVERLVENTEYEF RVKAKNDAGYSEPREAFSSVIIKEPQI EPTADLTGITNQLITCKAGSTFTIDVPI SGRPAPKVTWKLEEMRLKETDRVSI TTTKDRTTLSVKDSMRGDSGRYFLT LENTAGVKTFTITVVVIGRPGPVTGPI EVSSISAESCVLSWAEPQDNGGTDIT NYIVEKRESGTTAWQLVNSSVKRTQI KVTHLTKYMEYSFRVSSENRFGVSK PVESAPIVAEHPFVPPSAPTRPEVYYV SANAMSIRWEEPYHDGGSKVIGYWV EKKERNTILWVKENKVPCLECNYKV TGLVEGLEYQFRTYALNAAGVSKAS EASRPVMAQNPVDAPGRPEVTDVTR STVSLIWSAPVYDGGSKVVGYIIERK PVSEVGDGRWLKCNYTIVSDNFFTV TALSEGDTYEFRVLAKNAAGVISKG SESTGPITCRDEYAPPKAELDARLQG DLVTIRAGSDLVLDAAVGGKPEPKII WTKGDKELDMCEKISLQYTGKRAT AVIKFCDRSDSGKYTLTVKNASGTK AVSVLVKVLDSPGPCGKLTVSRVTE EKCTLAWSLPQEDGGAEITHYIVERR ETSRLNWVIVEGECPTLSHVVTRLIK NNEYIFRVRAVNKYGPGVPVESEPIV ARNSFTIPSQPGIPEEVGAGKEHIIIQ WTKPESDGGNDISNYLVDKREKKSL RWTRVNKDHVVYDTRLKVTGLMEG CDYQFRVTAVNAAGNSEPSEASNFIS CREPSYTPGPPSAPRVVDTTKHSISLA WTKPMYDGGTDIIGYVLEMQEKDT DQWYRVHTNATIRNNEFTVTDLKM GQKYSFRVAAVNVKGMSEYSESTAE IEPVERLEIPDLELADDLKKTVTIRAG ASLRLMVSVSGRPPPVITWSKKGIDL ASRAIIDTTESYSLLIVDKVNRYDAG KYTIEAENQSGKKSATVLVKVYDTP GPCPSVKVKEVSRDSVTITWEVPTID GGAPVNNYIIEKREAAMRAFKTVTT KCSKTLYRISGLIEGAMYYFRVLPEN IYGIGEPCETSDAVLVSEVPLVPTKLE VVDVTKSTVTLAWEKPLYDGGSRLT GYVLEACRAGTERWMKVVTLKPTV LEHTVISLNEGEQYLFRVRAQNEKG VSEPREIVTAVTVQDLRVLPTIDLST MPQKTIHVPAGRPVELVIPIAGRPPPA ASWFFAGSKLRESERVTVETHTKVA KLTIRETTIKDTGDYVLELKNVTGTT SETIKVIVLVDKPGPPSGPIKVDEIDA TSVTISWGPPELDGGAPLSGYVVEQR DAHRPGWLPVSESVTRSTFKFTRLIE GNEYVFRVAATNRFGIGSYLQSEVIE CRSSINIPGPPETLQIFDVSREGMTLT WYPPEDDGGSQVTGYIVERKEVRAD RWVRVNKVPVTMTRYRSTGLIEGLE YEHRVTAVNVRGTGKPSRPSKPTVA MDPIAPPGKPQNPRVTDTTRTSVSLA WSVPEDEGGSKVTGYLIEMQKVDQ HEWTKCNTTPTKIREYTLTHLPQGAE YRFRVLACNAGGPGEPAEVPGTVKV TEMLEYPDYELDERYQEGILVRQGG VIRLTIPIKGKPFPICKWTKEGQDISK RAMIATSETHTELVIKEADRDDSGTY DLILENKCGKKAVYIKVRVIGNPNTP EGPLEYDDIQARSVRVSWRPPADDG GADILGYILERREVPKSAWYTIDSRV RGTSLVVKGLKENVEYHFRVSAENQ FGISKPLKSEEPVIPKTPLNPPEPPSNP PEILDVTKSSVSLSWSRPKDDGGSRV TGYYIERKETSTDKWVRHNKTQITTT MYTVTGLVPDAEYQFRIIAQNDVGL SETSPASEPVVCKDPFDKPSQPGELEI LSISKDSVTLQWEKPECDGGKEILGY WVEYRQSGDSAWKKSSKDRIKDRQ FTIGGLLEATEYEFRVFAENETGLSRP RRTAMSVKTKLTSGEAPGVRKEMK DVTTKLGEAAQLSCQIVGRPLPDIKW FRFGKELVQSRKYKMSSDGRTHTLT VMTEEQEDEGVYTCVATNEVGEVES SSKLLLQATPQFHPGYPLKEKYYGA VGSTLRIHVMYIGRPVPAITWFRGQK LLQNSENITIENTEHYTHLVMKNVQR KTHAGKYKVQLSNVLGTVDTMLDV EIQDKPDKPTGPIVIEALLKNSVVISW KPPADDGGSWITNYVVEKCEAKEGA EWQLVSSAISVTTCRIVNLTENAGYY FRVSAQNMFGISEPLEVSSVVIIKSPF EKPGAPGKPTITAVTKDSCVVAWKP PASDGGAKIRNYYLEKREKKQNKWI AVTTDEIRETVFSVQNLIEGLEYEFR VKCENLGGESEWSEISEPVTPKSDVPI QAPHFKEELRNLNVRYQSNATLVCK VTGHPKPIVKWYRQGKEIIADGLKY RIQEFKGGYHQLIIASVTDDDATVYQ VRATNQGGSVSGTASLEVEDTVPAK IHLPKNLEGMGAVHALRGEVISIKIPF SGKPDPVITWQKGQDLIDNNGHYQV IVTRSFTSLVFPNGVERKDAGFYVVC AKNRFGIDQKTVELDVADVPDPPRG VKVSDVSRDSVNLTWTEPASDGGSK VTNYIVEKCATTAERWIRVGQARET RYTVINLFGKTSYQFRIIAENKFGLSK PSEPSEPTVTKEDKTRAMNYDEEVD ETREVSMTKASHSSTKELYEKYMIA EDLGRGQFGIVHRCVETSSKKTYMA KFVKVKGTDQVLVKKEISILNIARHR NMLYLHESFESMEELVMIFEFISGLDI FERINTSAFELNEREIVSYVRQVCEAL EFLHSHNIGHFDIRPDNIIYQTRRSSTI KIIEFGQARQLKPGDNFRLQFTAPEY YAPEVHQHDVVSTATDMWSLGTLV YVLLSGINPFLAETNQQIIENIMNAEY TFDEEAFQEISLEAMDFVDRLLVKER KSRMTASEALQHPWLKQKTERVSTK VIRTLKHRRYYHTLVKKDLNMVVSA ARISCGGAIRSQKGVSIAKVKVASIEI GPVSGQIMHAVGEEGGYVKYVCRIE NYDQSTQVTWYFGVRQLENSEKYEI TYEDGVATMYVKDITKFDDGTYRC KVVNDYGEDSSYAELFVKGVREVY DYYCRRTVKKLKRRTDAMRLLERPP EFTLPLYNKTAYVGENVRFGVTITVH PEPRVTWYKSGQKIKPGDDDRKYIFE SDKGLYQLTINSVTMDDDAEYTVVA RNKYGEDSCKAKLTVTPHPPPSDTTL RPMFKRLLANAECQEGQSVCFEIRVS GIPPPTLKWEKDGRPLSLGPNIEIIHE GLDYYALHIRDTLPEDTGYYRVTAT NTAGSSSCQAHLQVERLRYVKQEFK TKEEHERHVQRQIDKTLRMAEILSGT EAVPLTQVAQEALREAAILYKPAVST KTVKGEYRLETEEKKEERKLRMPYE VPEPRKYKQTTIEEDQHIKQFVPMSD MKWYKKIRDQFEMPGKIDRVVQKR PKRIRLSRWEQFYVMPLPRITDQYRP KWRIPKLSQDDLEMVRPARRRTPSP DYYYYYRPRRRSLGDISDEELLLPID DYLAMKRTEEERLRLEEELELGFSAS PPSRSPPRFELSSLRYSSPQAHVKVEE ARRDFRYSTYHIPTKEETSTSYAELR ERHARASHRQAHQRQRIMAEREDHE LLRPATTTQRLLEYKSELDHLAKEA KSRKKSRRQREVTEITEIEEEYEISKH AQRETSSSVSRLLRRRRSLSPTYIELM RPVSELIRPRPRPAEEYEDEEEEEEED VERRSPTPERTRPRSPSPVSSERSLSR FERSARFDIFSRYESMKAALKTQKTS ERKYEVLSQQPFTLDHAPRITLRMRS HRVPCGQNTRFILNVQSKPTAEVKW YHNGVELQESSKIHYTNTSGVLTLEI LDCHIDDSGTYRAVCTNYKGEASDY ATLDVTGGDYTTYASQRRDEQVPRS VFPELTRTEAYAVSSFKKTSEMEASS SVREMKSLMTETRESLSSYEHHASA EMKSAAIEEKSLEEKSTHRKIKTTLA ARILTKPRSITVYEGESARFSCDTDGE PVPTVTWLRGGQVISTSARHQVTTS KYKSTFEISSVQASDEGNYSVVVENS DGRQEAQFTLTVQKARVTEKAVTSP PRVKSPEPRVKSPEGAKSPKRVKSPE PVTPHPKAVPPTETKPTPTEKVQQKP VAAPPKIIQSLKAEASKDIAKLTCVV ESSALCAKEVTWYKDGRRLKENGHF QFHYSADGTYELKILNLAESDRGEY VCEVSGEGGTSKTNFQFVGQAFKSIH EQVSSISETNKKAAPKTAEPTETKKT EPKAPAPISTKPVIVTGLQDTTVSSDS VAKFAVKVTGEPQPTVIWTKDGKAI SQGGKYKLSEDKGAFFLEIHKTDTSD SGLYTCTITNSAGSVSSSCKLTIKVVE DTEIQKVSAQKTSEITSQKKASVQEEI SKKALISEEIKTSEVKSHEKLALKEEA STVLISEKVKKSEAASLEKSVVHEEIT KTSQASEEIRTHAEIKAFSTQMSITAG QKVTLKANIAGATDVKWVLNGVEL SNSDEYRYGISGSDQTLTIRQAGHKD EGILTCIGKTSQGIIKCQYDLTLSKEL SDAPAFISQPRSQNVNEGQNVLFSCEI SGEPSPEIEWFKNNLPISISSNISVSRS RNVYSLEIRNASVSDSGKYTIKAKNF HGQCSATASLTVLPLVEEPPREVVLR TSGDTSLQGSFSSQSVQMSASKQEAS FSSFSSSSASSMTEMKFASMSAQSMS SMQESFVEMSSSSFMGKSSMTQLESS TSRMLKAGLRGIPPKIEALPSDISIDE GKVLTVACAFTGEPTPEITWSRGGRT IHDQEQRGRFMENTDDL SEQ ID NP_990445.1 Troponin T, MSEAEEEYEEEQPEEEEEAAAAAEEE NO: 65 (NCBI) slow skeletal EEEEAEASKPHEEPEEERPRPRPVVP muscle/Gallus QLAPPKIPEGERVDFDDIHRKRMEKD gallus LLELQTLIDAHFEQRRREENELVALM ERIERRRAERNEQLRSRTEKERERQA RLAEEKLRKEEEEAKKRAEDDAKKK KVLSNMPHFGGYLAKAEQRRGKRQ TGREMKLRILAERKKPLHIEHMREDE LRAKAKELHDWIQQLESEKFDLMEK LRRQKYEINVLYNRISHAQKFKKVV GKGRVGGRWK SEQ ID Q98916 Slow muscle MSEAEEEYEEEQPEEEEEAAAAAEEE NO: 66 (UniProtKB) troponin EEEEAEASKPHEEPEEERPRPRPVVP T/Gallus gallus QLAPPKIPEGERVDFDDIHRKRMEKD LLELQTLIDAHFEQRRREENELVALM ERIERRRAERNEQLRSRTEKERERQA RLAEEKLRKEEEEAKKRAEDDAKKK KVLSNMPHFGGYLAKAEQRRGKRQ TGREMKLRILAERKKPLHIEHMREDE LRAKAKELHDWIQQLESEKFDLMEK LRRQKYEINVLYNRISHAQKFKKVV GKGRVGGRWK SEQ ID XP_419242.3 Troponin 1, slow MPEPRERKSKITASRKLLLKSLMLAK NO: 67 (NCBI) skeletal AKEEWEQEIVDKQSEKERYLSERITP muscle/Gallus LHTSGLSLSQLQDLCRELHEKVEIVD gallus EERYDIEAKCNHNTREIKDLKLKVLD LRGKFKRPPLRRVRVSADAMLRALL GSKHKVSMDLRANLKSVKKEDTEK ERPVEVGDWRKNVEAMSGMEGRKK MFDAAKSPTGQ SEQ ID F1NUT9 Troponin 11, LPIHTSTVCLCPQSLMLAKAKEEWE NO: 68 (UniProtKB) slow skeletal QEIVDKQSEKERYLSERITPLHTSGLS type/Gallus LSQLQDLCRELHEKVEIVDEERYDIE gallus AKCNHNTREIKDLKLKVLDLRGKFK RPPLRRVRVSADAMLRALLGSKHKV SMDLRANLKSVKKEDTEKERPVEVG DWRKNVEAMSGMEGRKKMFDAAK SPTGQ SEQ ID NP_001001862.1 Troponin C, MTDQQAEARSYLSEEMIAEFKAAFD NO: 69 (NCBI) skeletal MFDADGGGDISVKELGTVMRMLGQ muscle/Sus TPTKEELDAIIEEVDEDGSGTIDFEEF scrofa LVMMVRQMKEDAKGKSEEELAECF RIFDRNADGYIDAEELAEIFRASGEH VTDEELESLMKDGDKNNEGRIDFDE FLKMMEGVQ SEQ ID B5DH00 Fast myotomal MSDTEEVEAQKPQFKVPKIPDGDKV NO: 70 (UniProtKB) muscle troponin- DFDDIQKKRQNKDLIELQALIDAHFE T-1/Salmo salar HRKKEEEELIALKERIEKRRAERAEQ NRIRSEKEKERAARREEERLKREEAD AKKKADEDAKKKSALSSMGSNYSS HLQKADSKRGGKKETEREKKKKILA GRRKALNIDHLNEEKLKEKAKELHE WMQTLESEKFDHIERLKRQKYEVTT LRKRVEELSKFSKKGKTVRRK SEQ ID O57559 Troponin T MSDTEEVEHGEAHEAEEVHEEAHHE NO: 71 (UniProtKB) variant TnTx7- EAHHEEAHHEEAHHAEAHHAEAHH e16/Gallus EEAHAHAEEVHEPAPPPEEKPRIKLT gallus APKIPEGEKVDFDDIQKKRQNKDLIE LQALIDSHFEARRKEEEELVALKERI EKRRAERAEQQRIRAEKEKERQARL AEEKARREEEDAKRKAEDDLKKKK ALSSMGASYSSYLAKADQKRGKKQ TARETKKKVLAERRKPLNIDHLNED KLRDKAKELWDWLYQLQTEKYDFA EQIKRKKYEILTLRCRLQELSKFSKK AGAKGKVGGRWK SEQ ID A0A287BD71 Myotubularin MASAPTSKYNSHSLENESIKRTSRDG NO: 72 (UniProtKB) 1/Sus scrofa VNRDMGEAVPRLPGETPITDKEVIYI CPFNGPIKGRVYITNYRLYLRSLETD SALILDVPLGVISRIEKMGGATSRGE NSYGLDITCKDMRNLRFALKQEGHS RRDMFEILTRYAFPLAHSLPMFAFLN EEKFNVDGWTVYNPVEEYRRQGLP NHHWRITFINKCYELCDTYPALLVVP YRAADEDLRRVATFRSRNRIPVLSWI HPENKTVIVRCSQPLVGMSGKRNKD DEKYLDVIRETNRQVNKLTIYDARP NVNAVANKATGGGYESDDAYHNAE LFFLDIHNIHVMRESLKKVKDIVYPN VEESHWLSSLESTHWLEHIKLVLTGA IQVADRVSSGKSSVVVHCSDGWDRT AQLTSLAMLMLDSFYRSIEGFEILVQ KEWISFGHKFASRIGHGDKNHADAD RSPIFLQFIDCVWQMSKQFPTAFEFN EHFLITILDHLYSCRFGTFLYNCESAR ERQKVTERTVSLWSLINSNKDKFKN PFYTKEINRVLYPVASMRHLELWVN YYIRWNPRIKQQQPNPVEQRYMELL ALRDEYIKRLEELQLANSAKLSEPAA APSSPSQMVSHVQTHF SEQ ID Q4PS85 Myozenin-1/Sus MPLSGTPAPNKKRKSSKLIMELTGG NO: 73 (UniProtKB) scrofa GQESSGLNLGKKISVPRDVMLEELSL LTNRGSKMFKLRQMRVEKFIYENHP DVFSDSSMDHFQKFLPTVGGQLGTA GQGFSYSKGSSGGQAGGSSSAGQYG SGQQHHHQGSGSGSGGAGGPGSQTG RGGDAGTTGVGETGTGDQAGGEGK HITVFKTYISPWEKAMGVDPHQKVE LGIDLLAYGAKAELPQYKSFNRTAM PYGGYEKASKRMTFQMPKFDLGPLL SEPLVLYNQNLSNRPSFNRTPIPWLSS GEPVDYNVDIGIPLDGETEEL SEQ ID XP_010712691.1 myozenin-1 MPLAGTPAPLKRKKPTKLIGKLTHEV NO: 74 (NCBI) isoform MPQEVTKLNLGKKISIPRDVMLEELS X1/Meleagris LLTNKGSKMFKLRQLRVEKFIYENN gallopavo PDAFSDNSVDHFQRFIPSGGHYGEDA HGYGHGRMVGGVTAGQHGSSKQH YSTVPPRPGSKGGPGNSEGEHAEKSA GSAGEGGHGTEKDGKSGGKKPLLKT YISPWERAMGISPEDKSQLTIDLLSYS PKADFP HYKSFNRTAMPYGGYEKAAKRMTF KVPQFDICPLLPESIVLYNQNFRNRPS FNRTPIPWMPSGESSEYHTDINVPRS GETEEL SEQ ID Q0III9 Alpha-actinin- MMMVLQPEGLGTGEGPFAGGRGGG NO: 75 (UniProtKB) 3/Bos taurus EYMEQEEDWDRDLLLDPAWEKQQR KTFTAWCNSHLRKAGTQIENIEEDFR NGLKLMLLLEVISGERLPRPDKGKM RFHKIANVNKALDFIASKGVKLVSIG AEEIVDGNLKMTLGMIWTIILRFAIQ DISVEETSAKEGLLLWCQRKTAPYR NVNVQNFHTSWKDGLALCALIHRHR PDLIDYAKLRKDDPIGNLNTAFEVAE KYLDIPKMLDAEDIVNTPKPDEKAIM TYVSCFYHAFAGAEQAETAANRICK VLAVNQENEKLMEEYEKLASELLEW IRRTVPWLENRVGEPSMSAMQRKLE DFRDYRRLHKPPRVQEKCQLEINFNT LQTKLRLSHRPAFMPSEGKLVSDIAN AWRGLEQAEKGYEDWLLSEIRRLQR LQHLAEKFQQKASLH EAWTRGKEDMLSQRDYETASLQEV RALLRRHEAFESDLAAHQDRVEHIA ALAQELNELDYHEAASVNSRCQAIC DQWDNLGTLTQKRRDALERMEKLL ETIDQLQLEFARRAAPFNNWLDGAV EDLQDVWLVHSVEETQSLVTAHDQF KATLPEADRERGAILGIQGEIQKICQT YGLRPSSTNPYITLTPQDINTKWDTV RKLVPSRDQMLQEELTRQQVNERLR RQFAAQANAIGPWIQGKVEEVGRLA AGMAGSLEEQMAGLRQQEQNIINYK SNIDRLEGDHQLLQESLVFDNKHTV YSMEHIRVGWEQLLTSIARTINEVEN QVLTRDAKGLSQEQLNEFRASFNHF DRKRNGMMEPDDFRACLISMGYDL GEVEFARIMTMVDPNAAGVVTFQAF IDFMTRETAETDTAEQVVA SFKILAGDKNYITAEELRRELPAEQA EYCIRRMAPYKGAGAPAGALDYVAF SSALYGESDL SEQ ID G3X7I1 Alpha-actinin- MMMVLQPEGLGTGEGPFAGGRGGG NO: 76 (UniProtKB) 3/Bos taurus EYMEQEEDWDRDLLLDPAWEKQQR KTFTAWCNSHLRKAGTQIENIEEDFR NGLKLMLLLEVISGERLPRPDKGKM RFHKIANVNKALDFIASKGVKLVSIG AEGEEVAGGGRWGWGRGTTICTSQ EQLIYQVETSAKEGLLLWCQRKTAP YRNVNVQNFHTSWKDGLALCALIHR HRPDLIDYAKLRKDDPIGNLNTAFEV AEKYLDIPKMLDAEDIVNTPKPDEK AIMTYVSCFYHAFAGAEQAETAANR ICKVLAVNQENEKLMEEYEKLASEL LEWIRRTVPWLENRVGEPSMSAMQR KLEDFRDYRRLHKPPRVQEKCQLEIN FNTLQTKLRLSHRPAFMPSEGKLVSD IANAWRGLEQAEKGYEDWLLSEIRR LQRLQHLAEKFQQKASLH EAWTRGKEDMLSQRDYETASLQEV RALLRRHEAFESDLAAHQDRVEHIA ALAQELNELDYHEAASVNSRCQAIC DQWDNLGTLTQKRRDALERMEKLL ETIDQLQLEFARRAAPFNNWLDGAV EDLQDVWLVHSVEETQSLVTAHDQF KATLPEADRERGAILGIQGEIQKICQT YGLRPSSTNPYITLTPQDINTKWDTV RKLVPSRDQMLQEELTRQQVNERLR RQFAAQANAIGPWIQGKVEEVGRLA AGMAGSLEEQMAGLRQQEQNIINYK SNIDRLEGDHQLLQESLVFDNKHTV YSMEHIRVGWEQLLTSIARTINEVEN QVLTRDAKGLSQEQLNEFRASFNHF DRKRNGMMEPDDFRACLISMGYDL GEVEFARIMTMVDPNAAGVVTFQAF IDFMTRETAETDTAEQVVA SFKILAGDKNYITAEELRRELPAEQA EYCIRRMAPYKGAGAPAGALDYVAF SSALYGESDL SEQ ID Q3ZC55 Alpha-actinin- MNQIEPGVQYNYVYEDDEYMIQEEE NO: 77 (UniProtKB) 2/Bos taurus WDRDLLLDPAWEKQQRKTFTAWCN SHLRKAGTQIENIEEDFRNGLKLMLL LEVISGERLPKPDRGKMRFHKIANVN KALDYIASKGVKLVSIGAEEIVDGNV KMTLGMIWTIILRFAIQDISVEETSAK EGLLLWCQRKTAPYRNVNIQNFHTS WKDGLGLCALIHRHRPDLIDYSKLN KDDPIGNINLAMEIAEKHLDIPKMLD AEDIVNTPKPDERAIMTYVSCFYHAF AGAEQAETAANRICKVLAVNQENER LMEEYERLASELLEWIRRTIPWLENR TPEKTMQAMQKKLEDFRDYRRKHK PPKVQEKCQLEINFNTLQTKLRISNRP AFMPSEGKMVSDIAGAWQRLEQAE KGYEEWLLNEIRRLERVEHLAEKFR QKASTHETWAYGK EQILLQKDYESSTLTEVRALLRKHEA FESDLAAHQDRVEQIAAIAQELNELD YHDAVNVNDRCQKICDQWDRLGTL TQKRREALERTEKLLETIDQLHLEFA KRAAPFNNWMEGAMEDLQDMFIVH SIEEIQSLITAHEQFKATLPEADGERQ SILAIQNEVEKVIQSYSIRISSSNPYST VTVDEIRSKWDKVKQLVPIRDQSLQ EELARQHANERLRRQFAAQANAIGP WIQNKMEEIARSSIQITGALEDQMNQ LKQYEHNIINYKNNIDKLEGDHQLIQ EALVFDNKHTNYTMEHIRVGWELLL TTIARTINEVETQILTRDAKGITQEQM NEFRASFNHFDRRKNGLMDHEDFRA CLISMGYDLGEAEFARIMTLVDPNG QGTVTFQSFIDFMTRETADTDTAEQV IASFRILAS DKPYILAEELRRELPPDQAQYCIKRM PAYSGPGSVPGALDYTAFSSALYGES DL SEQ ID A0A1S3L6D5 SALSA M- MATKVMHFNQKKHVSHVSHHSSHT NO: 78 (UniProtKB) protein, striated TKHVVKEQSSRKSSSKSVNQVQHTH muscle isoform ATEAMAEPAYTVPAFRQRSADEIEE X3/Salmo salar YQRVSSHVGKGLASIQKELHRMRVV TKTQVENIAIKREVQEMMHKKMTLS TETDKAPDFMVALRPHTVWEKTPVK LFCTVDGHPRPIVKWYKGGKPVDPL SAPGKYKIESKYGVHSLIISRCMVSD TAEYSAVATNSHGSTTSKASVIVKRP AGGAYGSCQLFGQVPHLTVIHHSKL EITMLDRFGVSFGTEGGSISLVCTMV VVPDLPNVPPLAQWYRDDKLLKAG KLAEIKVGGGAATLTLPHLAKDDEG LYTLRMWTKDGTTEHSAYLFVKDA APSVAGAPGAPISVKAFDINSDYVLV AWKPPNTTNEAAITGYFVDKRESGS ATWSQCNDAPVQICKYPVH GLNVGHAYHFRVRAVNSAGISRPSR ESDKVTALDPAERERLQVIKLDGKH EVVIKDDDLEGVPSAPGQVVATRNT KSSVFVQWDPPKHPNHLMGYYIDAS LVGSKTWAPCNHKPYKNTRFVVHG LTPGETYVFRVQAVNVYGLSDESQE STPIAVEPALTTPSAPHGITMLSCDGA SMIIAWNSPKHRGGSKINAYYVDKR DADSLAWKEVNSAPTNTRTYTVDGL TEGTFYEFKVQAGNLAGVGVPSAPS TPLKCEAWTSAVPGPAYDLAFREVR GDSLVILWKAPVYTGASAVTGYIVE MAKKGHHYHPLNEEAIGHCYLQVT GLEAGAEYTFKVKAVNAEGVGKAS QTSEPVFAKALPGTQEIQCGVDEDTG DIFLSFEACEISETSNFAWSKNYKEIS DCTRVSIETKGKHSKLTF VNPDVSDLGAFSVHVTDTDGVSASH TVTEDALNTMLELSYNIRHPIVPLKH QLNYEVLEKGHVRFWLQCLKMSPA VNYRFIVNDKEVTSSDSHKISHDVNT GVIQMTVDHFSKASEATYTVQIHDG RAKNQSSLVLVGDVFKAALKEAEFQ RSEHIRKQGPHFAEYLSYHVDDDCT VLLVCKVANVKKETVFHWFKDEEEI VPETPPNVMSGSVALPISLFSRKDQG HYKAKLSDDRGKDTSVFDISGQVFD DIINAIAHIAGSSASELVLQCTPEGIRL QCYMKYYTEEIRTAWLHKDSKISSSE KMRIAGTAEMAWMQIREPSEKEKG HYSIQIMDATKSHTRTFDLSGQAYTD AYEEYLRLKAAAFAEKNRGRVVGG LPDVVTIMEQKTLSLTCTVWGDPSPE VTWFKNENEVVSTE HAKITLEANKFASLTITAVTSEDSGK YSINVRNKYGGEFVEITVSVYKQGES PPEPKMGGRGATPAPLASKTPAKTPT PAQTPTPSLKSPTPSLKSPTPSLRSPA KSPTPTPKSPTPPRRVKSPSPARSLKS PTPPRK SEQ ID P19352 Tropomyosin MEAIKKKMQMLKLDKENAIDRAEQ NO: 79 (UniProtKB) beta AEADKKQAEDRCKQLEEEQQGLQK chain/Gallus KLKGTEDEVEKYSESVKEAQEKLEQ gallus AEKKATDAEAEVASLNRRIQLVEEE LDRAQERLATALQKLEEAEKAADES ERGMKVIENRAMKDEEKMELQEMQ LKEAKHIAEEADRKYEEVARKLVVL EGELERSEERAEVAESKCGDLEEELK IVTNNLKSLEAQADKYSTKEDKYEE EIKLLGEKLKEAETRAEFAERSVAKL EKTIDDLEDEVYAQKMKYKAISEEL DNALNDITSL SEQ ID Q3ZC09 Beta- MAMQKIFAREILDSRGNPTVEVDLH NO: 80 (UniProtKB) enolase/Bos TAKGRFRAAVPSGASTGIYEALELRD taurus GDKSRYLGKGVLKAVEHINKTLGPA LLEKKLSVVDQEKVDKFMIELDGTE NKSKFGANAILGVSLAVCKAGAAEK GVPLYRHIADLAGNPELILPVPAFNVI NGGSHAGNKLAMQEFMILPVGASSF REAMRIGAEVYHHLKGVIKAKYGK DATNVGDEGGFAPNILENNEALELL KTAIQAAGYPDKVVIGMDVAASEFY RNGKYDLDFKSPDDPARHISGEKLG ELYKNFIKNYPVVSIEDPFDQDDWAT WTSFLSGVNIQIVGDDLTVTNPKRIA QAVEKKACNCLLLKVNQIGSVTESIQ ACKLAQSNGWGVMVSHRSGETEDT FIADLVVGLCTGQIKTGAPCRSERLA KYNQLMRIEEALGDKAVFAGRKFRN PKAK SEQ ID Q1AG05 Calsarcin 3/Sus MIPKEQKGPVVTAMGDLTEPAPLLD NO: 81 (UniProtKB) scrofa LGKKLSVPQDLMVEELSLPNNRGSL LFQERQRRVQKFTFEFAGSQRASAA GTAQGTVTATATPGRAANSPEGQNY SSELHVSLESPGGPEDVQPAAPRAVS APRPSALAPGYAEPLKGIPPEKFNHT AIPKGYRCPWQEFISYRDYLGEGRSH TPSLADYRNFNKTPVPFGGLLAGETL PRAGTPSVPELSSGLELLRLRPSFNRV AQGWVRNLPESEEL SEQ ID Q1AG02 Calsarcin MPLSGTPAPNKKRKSSKLIMELTGG NO: 82 (UniProtKB) 2/Oryctolagus GQESSGLNLGKKISVPRDVMLEELSL cuniculus LTNRGSKMFKLRQMRVEKFIYENHP DVFSDSSMDHFQKFLPTVGGQLGTA GQGVSYSKGSGGGQAGGSGSAGQY GSDHQHHHGSGSGAGGAGGPGGQA GKGGAAGAGGVGETGSGDQTGGDG KHITVFKTYISPWEQPWGVDPQQKV ELGIDLLAYGAKAELPKYKSFNRTA MPYGGYEKASKRMTFQMPKFDLGP LLSEPLVLYNQNLSNRPSFNRTPIPW LSSGEHADYHVDIGIPLDGETEEL SEQ ID A6QLT4 Myotubularin MASAPTSKYNSHSLENESIKRTSRDG NO: 83 (UniProtKB) OS = Bos taurus VNRDVGETLPRLPGEIRITDKEVIYIC PFNGPIKGRVYITNYRLYLRSLETDS ALILDVPLGVISRIEKMGGATSRGEN SYGLDITCKDLRNLRFALKQEGHSRR DMFEILTRYAFPLAHSLPIFAFLNEEK FNVDGWTVYNPVEEYRRQGLPNHH WRITFINKCYKLCDTYPALLVVPYRA SDEDLRRVATFRSRNRIPVLSWIHPE NKTVIVRCSQPLVGMSGKRNKEDER YLDVIRETNRQVNKLTIYDARPNVN AVANKATGGGYESDDVYHNAELFF LDIHNIHVMRESLKKVKDIVYPNVEE SHWLSSLESTHWLEHIKLVLTGAIQV ADRVSSGKSSVVVHCSDGWDRTAQ LTSLAMLMLDSFYRSIEGFEILVQKE WISFGHKFASRIGHGDKNHADADRS PIFLQFIDCVWQMSKQFPTAFEFNER FLITILDHLYSCRFGTFLYNCESAREK QKVTERTVSLWSLINSNKDKFKNPF YTKEINRVLYPVASMRHLELWVNYY IRWNPRIKQQQPNPVEQRYMELLAL RDEYIKRLDELQLANSAKLSDPSASP SSPSQMMPHVQTHF SEQ ID Q7YS81 Myogenin/Bos MELYETSPYFYQEPHFYDGENYLPV NO: 84 (UniProtKB) taurus HLQGFEPPGYERAELSLSPEARVPLE DKGLGPAEHCPGQCLPWACKVCKR KSVSVDRRRAATLREKRRLKKVNEA FEALKRSTLLNPNQRLPKVEILRSAIQ YIERLQALLSSLNQEERDLRYRGGGG PQAAVPSECSSHSASCSPQWGSALEF GPNPGDHLLPADPTDAHNLHSLTSIV DSITVEDVAAAFPDETIPN SEQ ID P49812 Myogenin/Sus MELYETSPYFYQEPHFYDGENYLPV NO: 85 (UniProtKB) scrofa HLQGFEPPGYERTELSLSPEARVPLE DKGLGTPEHCPGQCLPWACKVCKR KSVSVDRRRAATLREKRRLKKVNEA FEALKRSTLLNPNQRLPKVEILRSAIQ YIERLQALLSSLNQEERDLRYRGGGG PQPGVPSECSSHSASCSPEWGSALEF GPNPGDHLLTADPTDAHNLHSLTSIV DSITVEDVAVAFPDETMPN SEQ ID P31696-5 Agrin Isoform MGGSGAAATLALGLALGLALGGWA NO: 86 (UniProtKB) 5/Gallus gallus NCPERELQRREEEANVVLTGTVEEIM NVDPVHHTYSCKVRVWRYLKGKDI VTHEILLDGGNKVVIGGFGDPLICDN QVSTGDTRIFFVNPAPQYMWPAHRN ELMLNSSLMRITLRNLEEVEHCVEEH RKLLADKPNSYFTQTPPTPRDACRG MLCGFGAVCERSPTDPSQASCVCKK TACPVVVAPVCGSDYSTYSNECELE KAQCNQQRRIKVISKGPCGSKDPCAE VTCSFGSTCVRSADGQTAGCVCPAS CSGVAESIVCGSDGKDYRSECDLNK HACDKQENVFKKFDGACDPCKGILN DMNRVCRVNPRTRRVELLSRPENCP SKREPVCGDDGVTYASECVMGRTG AIRGLEIQKVRSGQCQHQDKCKDEC KFNAVCLKRWHARCSCDRITCDGTY RPVCARDSRTYSNDCERQKAECHQK AAIPVKHSGPCDLGTPSPCLSVECTF GATCVVKNREPVCECQQVCQGRYD PVCGSDNRTYGNPCELNAMACVLK REIRVKHKGPCDRCGKCQFGAICEAE TGRCVCPTECVPSSQPVCGTDGNTY GSECELHVRACTQQKNILVAAQGDC KSCGTTVCSFGSTCVGGQCVCPRCE QQPLAQVCGTDGLTYDNRCELRAAS CQQQKSIEVAKMGPCEDECGSGGSG SGDGSECEQDRCRHYGGWWDEDAE DDRCVCDFTCLAVPRSPVCGSDDVT YANECELKKTRCEKRQNLYVTSQGA CRALTTTPPPLPVVHCSQTIYGCCPD NMTLALGVGAAGCPSTCQCNPYGSY GGTCDPATGQCSCKPGVGGLKCDRC EPGFWNFRGIVTDSKSGCTPCNCDPV GSVRDDCEQMTGLCSCKTGITGMKC NQCPNGSKMGMAGCEKDPSAPKSC EEMSCEFGATCVEVNGFAHCECPSPL CSEANMTKVCGSDGVTYGDQCQLK TIACRQGQLITVKHVGQCHESITHTS HTMPPTPLPTLPLDKLIVPPPLQLTTQ APEPTELATTSLLMEASPTTRSHPTTR RVTTTRPVTTPWMTHGVLKTTVRPL STSPVVLATTQPPYAESGSAEGSGDQ EMSISGDQESSGAGSAGEEEVEESQV TPTPAIERATCYNTPLGCCSDGKTAA ADAEGSNCPATKVFQGVLILEEVEG QELFYTPEMADPKSELFGETARSIES ALDELFRNSDVKNDFKSIRVRDLGQS SAVRVIVESHFDPATSYTAADVQAA SLKQIRASKKRTILVKKPQQEHVKFM DFDWIPRIFTTTITTTTATTMAPATTR RHTTASAATTAHILRQDTVGHPSAK LAAPASTRRPTSTLPTTARRKPTRQP PSTTKKPSRPCDSHPCLHGGTCEDDG REFTCRCPAGKGGAVCEKPIRYFIPSF GGKSYLAFKMMKAYHTVRIAMEFR ATELSGLLLYNGQNRGKDFISLALVG GFVELRFNTGSGTGVITSKVRVEPGK WHQLVVNRNRRSGMLAVDGEHVSG ESPTGTDGLNLDTDLFVGGAPEDQM AVVAERTAATVGLKGSIRLLDVNNQ MYDLREKGSDVLYGSGVGECGNDP CHPNPCHHGASCHVKEAEMFHCECL HSYTGPTCADERNPCDPTPCHISATC LVLPEGGAMCACPMGREGEFCERVT EQDHTMPFLPEFNGFSYLELNGLQTL FLTCRQMSMEVVFLAKSPSGMIFYN GQKTDGKGDFVSLALHDGYLEYRY DLGKGAAVLRSKEPVPLNTWISVLL ERSGRKGVMRINNGERVMGESPVPH AFLNLKEPFYVGGAPDFSKLARAAAI STSFYGAVQRISIKGVPLLKEQHIRSA VEISTFRAHPCTQKPNPCQNGGTCSP RLESYECACQRGFSGAHCEKVIIEKA AGDAEAIAFDGRTYMEYHNAVTKSE KALQSNHFELSIKTEATQGLILWSGK GLERSDYIALAIVDGFVQMMYDLGS KPVVLRSTVPINTNHWTHIKAYRVQ REGSLQVGNEAPITGSSPLGATQLDT DGALWLGGMERLSVAHKLPKAYST GFIGCIRDVIVDRQELHLVEDALNNP TILHCSAK SEQ ID NP_001004406.1 Cofilin-2/Gallus MASGVTVNDEVIKVFNDMKVRKSST NO: 87 (NCBI) gallus PEEIKKRKKAVLFCLSDDKKQIIVEE ATRILVGDIGDTVEDPYTAFVKLLPL NDCRYALYDATYETKESKKEDLVFI FWAPESAPLKSKMIYASSKDAIKKKF TGIKHEWQVNGLDDIKDRSTLGEKL GGNVVVSLEGKPL SEQ ID P21566 Cofilin-2/Gallus MASGVTVNDEVIKVFNDMKVRKSST NO: 88 (UniProtKB) gallus PEEIKKRKKAVLFCLSDDKKQIIVEE AKQILVGDIGDTVEDPYTAFVKLLPL NDCRYALYDATYETKESKKEDLVFI FWAPESAPLKSKMIYASSKDAIKKKF TGIKHEWQVNGLDDIKDRSTLGEKL GGNVVVSLEGKPL SEQ ID Q679P3 PDZ and LIM MESYKVMLNGPAPWGFRLQGGKDF NO: 89 (UniProtKB) domain protein SMPLSISRLTPGGKAAQAGVGVGDW 7/Gallus gallus VLYIDGESTGTMTHIEAQNRIRACGD RLCLTLSRAQNHLGKPQKDSLPCSEP PKYNFAPSTALNKTARPFGASSPPNP RPGLVTKPVTYVPLAPACTPQHNGQ VSVPDPSPGAAMKTEPGLAPRTPAA TPGPTSRPPWAVDPSFAERYAPDKTS TVLSKHSQPATPTPMQNRSSIVQAAQ QAPESPGRTPLCYKCNKIIRGRYLVA LGHYYHPEEFTCCQCRKVLDEGGFF EEKGSIFCPKCYDTRYAPSCAKCKKK ITGEVMHALKMTWHVQCFTCAACK TPIRNRAFYMEEGQPYCERDYEKMF GTKCRGCDFKIDAGDRFLEALGFSW HDTCFVCAICQTNLEGKTFYSKKDK PLCKSHAFSHV SEQ ID F1NQD9 Radixin/Gallus MPKPINVRVTTMDAELEFAIQPNTTG NO: 90 (UniProtKB) gallus KQLFDQVVKTVGLREVWFFGLQYV DSKGYSTWLKLNKKVTQQDVRKEN PLQFKFRAKFFPEDVSEELIQEITQRL FFLQVKEAILNDEIYCPPETAVLLASY AVQSKYGDYNKEIHKLGYLANDRLL PQRVLEQHKLTKEQWEERIQNWHEE HRGMLREDSMMEYLKIAQDLEMYG VNYFEIKNKKGTELWLGVDALGLNI YEHDDKLTPKIGFPWSEIRNISFNDK KFVIKPIDKKAPDFVFYAPRLRINKRI LALCMGNHELYMRRRKPDTIEVQQ MKAQAREEKHQKQLERAQLENEKK KREIAEKEKERIEREKEELMERLRQIE EQTMKAQKELEEQTRRALELDQERK RAKEEAERLEKERRAAEEAKAALAK QAADQMKNQEQLAAELAEFTAKIAL LEEAKKKKEEEASEWQHKAFAAQE DLEKTKEELKSVMSAPPPPPPPPVIPP TENEHDEHDENNAEASAELSSDGVM NHRSEEERVTETQKNERVKKQLQAL SSELAQARDETKKTQNDVLHAENVK AGRDKYKTLRQIRQGNTKQRIDEFE AM SEQ ID XP_025008315.1 nebulin isoform MEEEEYEEVVEYYIEETIVEEGEPYE NO: 91 (NCBI) X22/Gallus VVTEITDSTSTEFTGPTTITRTIEYEKT gallus SGEGAATPVRKKTIRTKMDTSKFLTP YLQHSNKMKDLFSENKYKEKFNKER GKPYASTIDTPEIRRIKKVQEQLSEVK YRMAGEAARTICHVDEKAWDIEHA KKVSQQVSKVLYKQNWEENKDKYL LPPDAPELVNAIKNTAMFSKKLYTED WEGDKTLFYPYNDSPELRRVAQAQK ALSDIVYKKGHDERKSKYTSLPDPPD VEQAKKVTRQLSDIIYHDDYKNKIK GKWSQTPCYDVVIAKMNAENLSMK KYQEDFENVKDQIYFMQTETPEYEA NKRVSDNVSKIKYRADYEKNKAIAD YNVLPATENPLLRQLKTAGDVLSDK LYKEAYERSKGTSMNYCETPKFQTD NALKNFSDVKYKDAYQKNILGHYL GSFEDPHQIHCMKVEAMKSDKNYK ADYEEEKTKCYFPQTITQEYEAIKKL EQCKDHTYKKHPDQIKFTPVTDSPV QKQAEINSKQLSDKLYRSSGEEVKH KYTLPPDVPQFIQARYNAANVSDAY YKQDYHDLIAKGNNVSLDAIPITRAK ASRNIASDYKYKEAYEKAKGQQVGF KSLQDDPKLVHYMHVAKIQSDREYK KDYEKSKTNYHTPPDTFSIQAAKKSQ DVASTAHYKNLIHHYTYLPDAMDVE LAKNMMQIQSDNVYKQDYNSWFKG IGWSPLGSLDVEKAKKAGDALNEKK YRQHPDTIKFTSVPDSMTMVLAQHN TKQLSDVAYKQEGEKVKHKYKLDP DVPQFIQARVNAFNLSDANYKADW KKTIAKGYDLKPDAIPIIAAKASRNIA SDYKYKESYEKDKGRQVGYRSLQD DPKLVHYMHVAKMQSDREYKKDYE VTKTKYHTPLDMFSVTAAKKAQEA VTNTGYKQLIHHYTLLPDSVNLELSR NMMQLQSDNMYKADFNNWLRGVG WLPIQSLEVEKAKKASEILSEKKYRQ HPDKLKYSIPLDAMEQVLAKQNAKT MNKRLYTDKWNKEKTSIHVMPDTP EILQSRVNQITMSNKLYKAGWEEDK KKGYDMRPDAIPIKAAKTSQDIASDY KYKLAHEKAKGKHIGFRSLEDDPKL VHFMQVAKMQSDREYKKDYEKAKT NFHTPVDMLSVVAAKKAQEVATNA NYKNLIHVYNVLPDAMSLELAKNM MQIQSNNQYRAEYDESMKGVGWMP LGSLEAEKNKKAMEILSEKKYRQHP DKLKYSVPVDSMNMALALHNAKIM DEHQYKQAWEEDKKKVHMTPDIPQ FALAKANAFNISDKMYRHSFEEARK KGYDLRSDAIPIKAAKASRDIASDYK YKLGYEQDKGKLVGFRSLQDDPKLV HYMQVAKMQSDREYKKAYETSKTH YQTPSDALSIMAAKEAQDRVTNANY KRLIHHYMLLPDAMSFELYRNMNQI QSNNEYKQDYNEWFKGIGWSPAGSL DVEKSKKATEIASDQKYRQHPSIFPF TKQIDAMDMVLAKHNADIMNKHAY TQAWEKDKTQVHVMPDTPDILQAK QNKANYSQKQYKLDWQEMIKKGYD LTPEAISVKAAKASRDIASDYKYKEG YRKQQGHHIGFRSLQDDPKMMWSM QVAKMQSEREYKKDFEKWKTKFNM PVDMLGFLLAKKCQELVSDIDYKHM LHRWTCLPDQNDVTQAKRVYELQS DNLYKSDLQWLRGIGWSPLGSLESE KNKKASEILSEKKYRQHPDTIKFTSIP DAMNIILAKSNAKNRSDILYREAWD KDKTQVHIMPDTPEILLAKSNLINTS DKHYKLGYEELRRKGYDLPPDAIPL KSAKASRDIASEYQYKTAYRKQLGH HVGARNIEDDPKMMWSMHVAKIQS DREYKKAFEKTKTHFSSPVDMLGIV LAKKCQELVSDVDYKHLLHRWTCL PDQNDVVQARKVYDLQSDNVYKSD LQWLRGIGWSPLGSLDEEKNKRASM ILSDKKYRQHPDTIKFTSLPDSMPMV LAKHNSEIMNHRSYIAAWEKDKTSI HIMPDTPGILLAQQNKVNYSEKMYR LAMEEDKKKGYDLRADAIPIKAAKA SRDIASDYKYKEGYRKQLGHHIGAR NIEDDPKMMWSMHVAKVQSDREYK KAFEKTKTHFSSPVDMLGIVLAKKC QELVSDVDYKHLLHRWTCLPDQND VVQARKVYDLQSDNVYKSDLQWLR GIGWSPLGSLDEEKNKRASMILSDKK YRQHPDTIKFTSLPDSMPMVLAKHN SEIMNHRSYIAAWEKDKTSIHIMPDT PGILLAQQNKVNYSEKMYRLAMEED KKKGYDLRADAIPIKAAKASRDIASD YKYKEGYRKQLGHHIGARNIEDDPK MMWSMHVAKVQSDREYKKAFEKT KTHFSSPVDMLGIVLAKKCQELVSD VDYKHLLHRWTCLPDQNDVVQARK VYDLQSDNVYKSDLQWLRGIGWSPL GSLDEEKNKRASMILSDKKYRQHPD TIKFTSLPDSMPMVLAKHNSEIMNHR SYIAAWEKDKTSIHIMPDTPGILLAQ QNKVNYSEKMYRLAMEEDKKKGY DLRADAIPIKAAKASRDIASDYKYKE GYRKQLGHHIGARNIEDDPKMMWS MHVAKVQSDREYKKAFEKTKTHFSS PVDMLGIVLAKKCQELVSDVDYKHL LHRWTCLPDQNDVVQARKVYDLQS DNVYKSDLQWLRGIGWSPLGSLDEE KNKRASMILSDKKYRQHPDTIKFTSL PDSMPMVLAKHNSEIMNHRSYIAAW EKDKTSIHIMPDTPGILLAQQNKVNY SEKMYRLAMEEDKKKGYDLRADAI PIKAAKASRDIASDYKYKEGYRKQL GHHIGARNIEDDPKMMWSMHVAKI QSDREYKKAFEKTKTHFSSPVDMLGI VLAKKCQELVSDVDYRHYLHQWIC LPDQNDVIHARKAYDLQSDNFYKSD LEWMRGIGWVPIGSLDIEKAKRAGQI LSDKVYRQPPDTIKFTSVTDSLEMTL AKHNAEMMNKRLYTEAWDKDKTQI HIMPDTPEITLAKQNMHNYSEKLYK QAMEEAKKKGYDLRSDAIPIQAAKA SRQIASDYKYKEGYRKQLGHHIGAR NIEDDPKMMWSMHVAKIQSDREYK KAFEKTKTHFSSPVDMLGIVLAKKC QELVSDVDYRHYLHQWICLPDQND VIHARKAYDLQSDNFYKSDLEWMR GIGWVPIGSLDVEKAKRAGQILSDKV YRQPPDTIKFTSVTDSLEMTLAKHNA ETMNKRLYTEAWNKDKTTIHVMPD TPEILLAKQNQAHYSQKMYKLALEE SKKKGHDLRFDAIPIQAAKASREIAS DYKYKEGYRKQLGHHIGARNIEDDP KMMWSMHVAKIQSDREYKKAFEKT KTHFSSPVDMLGIVLAKKCQELVSD VDYRHYLHQWICLPDQNDVIHARKA YDLQSDAVYKSDLEWLKGIGWVPIG SLDVEKAKKAGEILSDRKYRQPADQI KFTSVTDSLAMMLAKHNAEIMNKRL YTEAWDADKTSIHVMPDTPTILLAK ANAANVSHKHYVQAWEDAKKKGY DMRADAIPIRSAKASRDIASDYKYKE AHEKQKGHYIGCRTAKEDPKLSWA ARAMLLQNDRIYRKAYNDSKAHIH MPVDAMSLQAAKECQTLVSDVDYR HYLHQWTCLPDQNDVMHARKAYD LQSDNVYKSDLEWLRGIGWLTEGSV DVIKAKKAQEILSDRLYRTQPDKMK FTSITDTPDVVQAKINAMQLSNHLYR EVWDKDKTQISIPSDTPELLQSKLNA LNISNKHYQKAWDEAKAKNYDLRA DAIPIKHAKASRDIASEYKYKEAHEK QKGHYIGCRTAKEDPKLSWAARAM LLQNDRIYRKAYNDSKAHIHMPVDA MSLQAAKECQTLVSDVDYRHYLHQ WTCLPDQNDVMHARKAYDLQSDNV YKSDLEWLRGIGWLTEGSVDVVKA KKAQEILSDRLYRTQPDKMKFTSVT DAPDVVQAKINAMQLSNRLYREAW DKDKTQISIPSDTPEMLQSKVNALNIS NKHYQKAWDEAKAKNYDLRADAIP IKHAKASRDIASEYKYKEAHEKQKG HYIGCRTAKEDPKLSWAARAMLLQ NDRIYRKAYNDSKAHIHMPVDAMSL QAAKECQTLVSDVDYRHYLHQWTC LPDHNDVVHARKAYDLQSDAVYKS DLEWLRGIGWLPNDSPGVQRVKHA QDLLSDKVYRTPIDSVKYTSVVDSPD ILLAKMNAEQLSIPKYKEAWEKDKT MIHIMPDTPEITLARSNAHNYSQKLY KEAWDEVKMSYDLRADAIPIKAAKA SREIASDYKYKLDHEKQKGHYVGVP NAKADTKIRFALGIGKVQSELEYKK HFAKWKTQCHLPVDMLSIQSAKHG QSLVSDVDYRHYLHQWICLPDQNDV IHARKAYDLQSDAVYKSDLEWLRGI GWLPNDSLGINHVKHAGDLLNERKY RTKAETLHFTPVADRVDYVTAKKSG EILSDIKYHKDWNEVKSNYTLTDTPQ LDMAREAARILNQSLYKESWEKEKA TGYLLPPDTVQIRHAKHSNDVQSEL KYKADYVKQRGHYVGVASMRDDP KLVWFEHAGEIQNDRLYKSNYHKTK SKIHIPADIMSVVAAKECQALVSDVD YRHYLHQWTCHPDQNDCIQARKAY DLQSDNIYKSDLEWLRGCGWIPLGS VEHKKVKHAQELINKRAYTKDAIEN FSKYTSVVDTPDIVLAKINSVNQSDL KYKETFNLEKGQYIGSDDTPELNHA RDMSLLYSDKLYKRDWEVCKPIGYT LDAKYIPLVGAKHANYVNSELKYKE IYEKLKGHYLAGKDIGDFPSVVHSLA FQKIRSALAYRKNYEDTKTRVHIPSD MMNHVLAKKCQYILSDLEYRTYLH HWNCSPEEHDVIQARRAQEILSDVV YKDDLNWLKGIGCYVWDTPQILHA KKSYDLQSQIKYTAAGKENFQNFGV VTDTPVYVTAVQSGINASDVKYKED YHKTKDKYTTVTETADSERVQNLKH LFSNNLYKEAWDRVKATSYIMPSDA VSLARAKELKHNASIVKYREEYDKF KALYTLPRSVEDDPNTARCLRVGKL NIDRLYKETYEKNKAKVHIIPDMVDI IAAKDAQKKISEIDYRTHLHDWICLP DLQINAHVRKVADQISDVVYKDDLN WLKGIGCFVWDTPEILHAKHAYDLR SDIKYKSDADKMKSKYTVVMDTPV YVQNILSGLNASEVIYRGDYLKKVR GKMIPTDKTVDLQRAHHANKIQSEN LYRWAGLKALPTGYSLPKDTPGFQH AKHVQHIGSDLKYKEAYEHMKAKG YTLGPNDVGFENVKKVNQVINERLY RATYHKNKDKIHTTPDTPEIRQVRAT QEAVSDLIYKSDFFKLQGHLISLPFTP QVLHCRYVGDITSDNKYKEDLKWL QGLGCFLYDTPDMVRARQLRKLWS NYVYTDSAKKMRDKYSVVLDTPGY RTVQELKTHLSDLVYRAAGKELKTK YTSVLNTPDFLRAKEGQRIQSQYLYV ALATKERPHHHAGNQTPAFTHARHV KDMVSETKYKIQYEKMKDKYTPVL DTPILIRAKRAYLNASDLRYKETFEN TKGKYHTVKDALDIVYHRKVTDDIS SVKYKENYMSQLGIWRSIPDRPEHFH HRAVTDAISDVKYKEDLSWLRGIGC YAWDTPDFALAEKNKVLYSGHKYK ETFEKTKSHFKYVADSPINRHFKHAT QLLDANSYKSLAKMLLKQGCNEILR PDILTALYNSYLWSQVEYKKDYEKK KDKYTTWDTPENIRTAKVNKQISDI IYKLEYNKAKPKGYTTIHDTPMLLH VRKVKDRISDLKYKELYERNKSHCN VVADSVHIKTPRHAYKLNSNLDYKK KYEAAKAHWHWIADRPDFVQAAKS SLQQSDYEYKLDREYLKGCKLSVTD DKNTVLALNNAILASDIKYKEKHNK ARGTCLVVPDTPQILLAKNVSSLVSE NKYKEHSKKQLPRGSYTTLPETRDT AHVKEVTKNVSDTNYKKKFVKEKG KSNYSIMLEPPEVKHAMEVAKKQST VEYKKDAKSKLHYTPIADRPDIKKA TQAAKLISDIEYKKRGEAGLGVTML GRPDIELAKEVSKLTSQVKYKENFSK EKGKKPKYDLKEAKIYKTMKDAHNI ASEVKYKADLKKLHKPVTDMSESLI MNHVLNTSQLASAVKYKEKYEKEK GKPMLDFETPTYLTAKESQLMQSEK EYRKDLEEGVKGKGLSVLEETPDML RAKNATQILNEKEYKKALELEIKGK GLSELALETPDFVRAKNATDIASQIK YKQLAEMEKANYTSVVDTPEIIHAQ QVKNLSSQKKYKEEAEKTMPYYVP VADTPEMQRVRENQKNFSTLQYQW DLQNSKGKVTVVQDTPEMLRVKEN QKNFSSIKYKESIGKGTPIPDLPEVKR VKETQKHISSVLYKEHLAKGTPTPM TPEMERAKRNQENISSVLYSDSFRKQ VQGKAAYVLDTPEMRRVRETQKHIS TVKYHEDFEKNKGTFTPVVTDPITER VKKNMHDFSDISYRGIQRRVVEMEQ KRVDQDQENLTGLRVWRTNPGSVF DYDPAEDNIQSRSLHMISVQAQRRSR EHSRSASALSISGGDEKSEPSEGVNQ HLSYYSSGGFFTTTATVGYKHAKTIE LPQQRSASVATQQTTVSSVPSHPSTA GKTYRAMYDYTAADADEVSFKDGD TIVNVQAIDEGWMYGTVQRTGKTG MLPANYVEAV SEQ ID XP_025008310.1 Nebulin isoform MEEEEYEEVVEYYIEETIVEEGEPYE NO: 92 (NCBI) X17/Gallus VVTEITDSTSTEFTGPTTITRTIEYEKT gallus SGEGAATPVRKKTIRTKMDTSKFLTP YLQHSNKMKDLFSENKYKEKFNKER GKPYASTIDTPEIRRIKKVQEQLSEVK YRMAGEAARTICHVDEKAWDIEHA KKVSQQVSKVLYKQNWEENKDKYL LPPDAPELVNAIKNTAMFSKKLYTED WEGDKTLFYPYNDSPELRRVAQAQK ALSDIVYKKGHDERKSKYTSLPDPPD VEQAKKVTRQLSDIIYHDDYKNKIK GKWSQTPCYDVVIAKMNAENLSMK KYQEDFENVKDQIYFMQTETPEYEA NKRVSDNVSKIKYRADYEKNKAIAD YNVLPATENPLLRQLKTAGDVLSDK LYKEAYERSKGTSMNYCETPKFQTD NALKNFSDVKYKDAYQKNILGHYL GSFEDPHQIHCMKVEAMKSDKNYK ADYEEEKTKCYFPQTITQEYEAIKKL EQCKDHTYKKHPDQIKFTPVTDSPV QKQAEINSKQLSDKLYRSSGEEVKH KYTLPPDVPQFIQARYNAANVSDAY YKQDYHDLIAKGNNVSLDAIPITRAK ASRNIASDYKYKEAYEKAKGQQVGF KSLQDDPKLVHYMHVAKIQSDREYK KDYEKSKTNYHTPPDTFSIQAAKKSQ DVASTAHYKNLIHHYTYLPDAMDVE LAKNMMQIQSDNVYKQDYNSWFKG IGWSPLGSLDVEKAKKAGDALNEKK YRQHPDTIKFTSVPDSMTMVLAQHN TKQLSDVAYKQEGEKVKHKYKLDP DVPQFIQARVNAFNLSDANYKADW KKTIAKGYDLKPDAIPIIAAKASRNIA SDYKYKESYEKDKGRQVGYRSLQD DPKLVHYMHVAKMQSDREYKKDYE VTKTKYHTPLDMFSVTAAKKAQEA VTNTGYKQLIHHYTLLPDSVNLELSR NMMQLQSDNMYKADFNNWLRGVG WLPIQSLEVEKAKKASEILSEKKYRQ HPDKLKYSIPLDAMEQVLAKQNAKT MNKRLYTDKWNKEKTSIHVMPDTP EILQSRVNQITMSNKLYKAGWEEDK KKGYDMRPDAIPIKAAKTSQDIASDY KYKLAHEKAKGKHIGFRSLEDDPKL VHFMQVAKMQSDREYKKDYEKAKT NFHTPVDMLSVVAAKKAQEVATNA NYKNLIHVYNVLPDAMSLELAKNM MQIQSNNQYRAEYDESMKGVGWMP LGSLEAEKNKKAMEILSEKKYRQHP DKLKYSVPVDSMNMALALHNAKIM DEHQYKQAWEEDKKKVHMTPDIPQ FALAKANAFNISDKMYRHSFEEARK KGYDLRSDAIPIKAAKASRDIASDYK YKLGYEQDKGKLVGFRSLQDDPKLV HYMQVAKMQSDREYKKAYETSKTH YQTPSDALSIMAAKEAQDRVTNANY KRLIHHYMLLPDAMSFELYRNMNQI QSNNEYKQDYNEWFKGIGWSPAGSL DVEKSKKATEIASDQKYRQHPSIFPF TKQIDAMDMVLAKHNADIMNKHAY TQAWEKDKTQVHVMPDTPDILQAK QNKANYSQKQYKLDWQEMIKKGYD LTPEAISVKAAKASRDIASDYKYKEG YRKQQGHHIGFRSLQDDPKMMWSM QVAKMQSEREYKKDFEKWKTKFNM PVDMLGFLLAKKCQELVSDIDYKHM LHRWTCLPDQNDVTQAKRVYELQS DNLYKSDLQWLRGIGWSPLGSLESE KNKKASEILSEKKYRQHPDTIKFTSIP DAMNIILAKSNAKNRSDILYREAWD KDKTQVHIMPDTPEILLAKSNLINTS DKHYKLGYEELRRKGYDLPPDAIPL KSAKASRDIASEYQYKTAYRKQLGH HVGARNIEDDPKMMWSMHVAKIQS DREYKKAFEKTKTHFSSPVDMLGIV LAKKCQELVSDVDYKHLLHRWTCL PDQNDVVQARKVYDLQSDNVYKSD LQWLRGIGWSPLGSLDEEKNKRASM ILSDKKYRQHPDTIKFTSLPDSMPMV LAKHNSEIMNHRSYIAAWEKDKTSI HIMPDTPGILLAQQNKVNYSEKMYR LAMEEDKKKGYDLRADAIPIKAAKA SRDIASDYKYKEGYRKQLGHHIGAR NIEDDPKMMWSMHVAKVQSDREYK KAFEKTKTHFSSPVDMLGIVLAKKC QELVSDVDYKHLLHRWTCLPDQND VVQARKVYDLQSDNVYKSDLQWLR GIGWSPLGSLDEEKNKRASMILSDKK YRQHPDTIKFTSLPDSMPMVLAKHN SEIMNHRSYIAAWEKDKTSIHIMPDT PGILLAQQNKVNYSEKMYRLAMEED KKKGYDLRADAIPIKAAKASRDIASD YKYKEGYRKQLGHHIGARNIEDDPK MMWSMHVAKVQSDREYKKAFEKT KTHFSSPVDMLGIVLAKKCQELVSD VDYKHLLHRWTCLPDQNDVVQARK VYDLQSDNVYKSDLQWLRGIGWSPL GSLDEEKNKRASMILSDKKYRQHPD TIKFTSLPDSMPMVLAKHNSEIMNHR SYIAAWEKDKTSIHIMPDTPGILLAQ QNKVNYSEKMYRLAMEEDKKKGY DLRADAIPIKAAKASRDIASDYKYKE GYRKQLGHHIGARNIEDDPKMMWS MHVAKVQSDREYKKAFEKTKTHFSS PVDMLGIVLAKKCQELVSDVDYKHL LHRWTCLPDQNDVVQARKVYDLQS DNVYKSDLQWLRGIGWSPLGSLDEE KNKRASMILSDKKYRQHPDTIKFTSL PDSMPMVLAKHNSEIMNHRSYIAAW EKDKTSIHIMPDTPGILLAQQNKVNY SEKMYRLAMEEDKKKGYDLRADAI PIKAAKASRDIASDYKYKEGYRKQL GHHIGARNIEDDPKMMWSMHVAKI QSDREYKKAFEKTKTHFSSPVDMLGI VLAKKCQELVSDVDYRHYLHQWIC LPDQNDVIHARKAYDLQSDNFYKSD LEWMRGIGWVPIGSLDVEKAKRAG QILSDKVYRQPPDTIKFTSVTDSLEM TLAKHNAETMNKRLYTEAWNKDKT TIHVMPDTPEILLAKQNQAHYSQKM YKLALEESKKKGHDLRFDAIPIQAAK ASREIASDYKYKEGYRKQLGHHIGA RNIEDDPKMMWSMHVAKIQSDREY KKAFEKTKTHFSSPVDMLGIVLAKK CQELVSDVDYRHYLHQWICLPDQND VIHARKAYDLQSDAVYKSDLEWLK GIGWVPIGSLDVEKAKKAGEILSDRK YRQPADQIKFTSVTDSLAMMLAKHN AEIMNKRLYTEAWDADKTSIHVMPD TPTILLAKANAANVSHKHYVQAWE DAKKKGYDMRADAIPIRSAKASRDI ASDYKYKEAHEKQKGHYIGCRTAKE DPKLSWAARAMLLQNDRIYRKAYN DSKAHIHMPVDAMSLQAAKECQTL VSDVDYRHYLHQWTCLPDQNDVMH ARKAYDLQSDNVYKSDLEWLRGIG WLTEGSVDVIKAKKAQEILSDRLYR TQPDKMKFTSITDTPDVVQAKINAM QLSNHLYREVWDKDKTQISIPSDTPE LLQSKLNALNISNKHYQKAWDEAK AKNYDLRADAIPIKHAKASRDIASEY KYKEAHEKQKGHYIGCRTAKEDPKL SWAARAMLLQNDRIYRKAYNDSKA HIHMPVDAMSLQAAKECQTLVSDV DYRHYLHQWTCLPDQNDVMHARK AYDLQSDNVYKSDLEWLRGIGWLTE GSVDVVKAKKAQEILSDRLYRTQPD KMKFTSVTDAPDVVQAKINAMQLS NRLYREAWDKDKTQISIPSDTPEMLQ SKVNALNISNKHYQKAWDEAKAKN YDLRADAIPIKHAKASRDIASEYKYK EAHEKQKGHYIGCRTAKEDPKLSWA ARAMLLQNDRIYRKAYNDSKAHIH MPVDAMSLQAAKECQTLVSDVDYR HYLHQWTCLPDHNDVVHARKAYDL QSDAVYKSDLEWLRGIGWLPNDSPG VQRVKHAQDLLSDKVYRTPIDSVKY TSVVDSPDILLAKMNAEQLSIPKYKE AWEKDKTMIHIMPDTPEITLARSNAH NYSQKLYKEAWDEVKMSYDLRADA IPIKAAKASREIASDYKYKLDHEKQK GHYVGVPNAKADTKIRFALGIGKVQ SELEYKKHFAKWKTQCHLPVDMLSI QSAKHGQSLVSDVDYRHYLHQWICL PDQNDVIHARKAYDLQSDAVYKSDL EWLRGIGWLPNDSLGINHVKHAGDL LNERKYRTKAETLHFTPVADRVDYV TAKKSGEILSDIKYHKDWNEVKSNY TLTDTPQLDMAREAARILNQSLYKES WEKEKATGYLLPPDTVQIRHAKHSN DVQSELKYKADYVKQRGHYVGVAS MRDDPKLVWFEHAGEIQNDRLYKS NYHKTKSKIHIPADIMSVVAAKECQ ALVSDVDYRHYLHQWTCHPDQNDC IQARKAYDLQSDNIYKSDLEWLRGC GWIPLGSVEHKKVKHAQELINKRAY TKDAIENFSKYTSVVDTPDIVLAKINS VNQSDLKYKETFNLEKGQYIGSDDT PELNHARDMSLLYSDKLYKRDWEV CKPIGYTLDAKYIPLVGAKHANYVN SELKYKEIYEKLKGHYLAGKDIGDFP SVVHSLAFQKIRSALAYRKNYEDTK TRVHIPSDMMNHVLAKKCQYILSDL EYRTYLHHWNCSPEEHDVIQARRAQ EILSDVVYKDDLNWLKGIGCYVWDT PQILHAKKSYDLQSQIKYTAAGKENF QNFGVVTDTPVYVTAVQSGINASDV KYKEDYHKTKDKYTTVTETADSERV QNLKHLFSNNLYKEAWDRVKATSYI MPSDAVSLARAKELKHNASIVKYRE EYDKFKALYTLPRSVEDDPNTARCL RVGKLNIDRLYKETYEKNKAKVHIIP DMVDIIAAKDAQKKISEIDYRTHLHD WICLPDLQINAHVRKVADQISDVVY KDDLNWLKGIGCFVWDTPEILHAKH AYDLRSDIKYKSDADKMKSKYTVV MDTPVYVQNILSGLNASEVIYRGDY LKKVRGKMIPTDKTVDLQRAHHAN KIQSENLYRWAGLKALPTGYSLPKD TPGFQHAKHVQHIGSDLKYKEAYEH MKAKGYTLGPNDVGFENVKKVNQV INERLYRATYHKNKDKIHTTPDTPEI RQVRATQEAVSDLIYKSDFFKLQGH LISLPFTPQVLHCRYVGDITSDNKYK EDLKWLQGLGCFLYDTPDMVRARQ LRKLWSNYVYTDSAKKMRDKYSVV LDTPGYRTVQELKTHLSDLVYRAAG KELKTKYTSVLNTPDFLRAKEGQRIQ SQYLYVALATKERPHHHAGNQTPAF THARHVKDMVSETKYKIQYEKMKD KYTPVLDTPILIRAKRAYLNASDLRY KETFENTKGKYHTVKDALDIVYHRK VTDDISSVKYKENYMSQLGIWRSIPD RPEHFHHRAVTDAISDVKYKEDLSW LRGIGCYAWDTPDFALAEKNKVLYS GHKYKETFEKTKSHFKYVADSPINR HFKHATQLLDANSYKSLAKMLLKQ GCNEILRPDILTALYNSYLWSQVEYK KDYEKKKDKYTTVVDTPENIRTAKV NKQISDIIYKLEYNKAKPKGYTTIHD TPMLLHVRKVKDRISDLKYKELYER NKSHCNVVADSVHIKTPRHAYKLNS NLDYKKKYEAAKAHWHWIADRPDF VQAAKSSLQQSDYEYKLDREYLKGC KLSVTDDKNTVLALNNAILASDIKY KEKHNKARGTCLVVPDTPQILLAKN VSSLVSENKYKEHSKKQLPRGSYTTL PETRDTAHVKEVTKNVSDTNYKKKF VKEKGKSNYSIMLEPPEVKHAMEVA KKQSTVEYKKDAKSKLHYTPIADRP DIKKATQAAKLISDIEYKKRGEAGLG VTMLGRPDIELAKEVSKLTSQAEYL KRHMRGHGAASYDTPWMRTFKKA NMLSSHVKYKENFSKEKGKKPKYDL KEAKIYKTMKDAHNIASEVKYKADL KKLHKPVTDMSESLIMNHVLNTSQL ASAYQYKKQYEKSKGHYHMVPDNL EQVHLREASELQSHVKYKEKYEKEK GKPMLDFETPTYLTAKESQLMQSEK EYKRDFEESIKGRNLTGLEITPSLLHV KYATKIASEKEYRKDLEEGVKGKGL SVLEETPDMLRAKNATQILNEKEYK KALELEIKGKGLSELALETPDFVRAK NATDIASQIKYKQLAEMEKANYTSV VDTPEIIHAQQVKNLSSQKKYKEEAE KTMPYYVPVADTPEMQRVRENQKN FSTLQYQWDLQNSKGKVTVVQDTPE MLRVKENQKNFSSILYKEDIGTGTTI EKTPEMQRVKRTQDAISSIKYKESIG KGTPIPDLPEVKRVKETQKHISSVLY KEDLGTGIPTPVTPEIERVKRNQEHIS SVLYKEELGTGTPIPVTPEVERVKRN QEHISSVLYKESVGTGTPTPITPEMER VKRNQEICSSVLYKENIGKATPTPVT PEMERVKRNQEIISSVLYKENVGKVT PTPITPEMERVKRNQEIISSVLYKESL GRATPTPITPEMERVKRNQEQISSVV YKEGLGKATPTPVTPEMERVRRNQE QISSVLYKEHLAKGTPTPMTPEMERA KRNQENISSVLYSDSFRKQVQGKAA YVLDTPEMRRVRETQKHISTVKYHE DFEKNKGTFTPVVTDPITERVKKNM HDFSDISYRGIQRRVVEMEQKRVDQ DQENLTGLRVWRTNPGSVFDYDPAE DNIQSRSLHMISVQAQRRSREHSRSA SALSISGGDEKSEPSEGVNQHLSYYS SGGFFTTTATVGYKHAKTIELPQQRS ASVATQQTTVSSVPSHPSTAGKTYR AMYDYTAADADEVSFKDGDTIVNV QAIDEGWMYGTVQRTGKTGMLPAN YVEAV SEQ ID F1MQI3 Nebulin/Bos MADDEEYEEVVEYYTEETVYEEVPG NO: 93 (UniProtKB) taurus ETRTRFYETTTTRTSDYEQSETSRPA LAQPVPEKPVERKRVIRKKVDPSKF MTPYIAHSQKMQNLFSTNKYKENYE KAKGKPYAITTDTPELRRIKKVQDQL SEVKYRVDGDVAKTICHVDEKAKDI EHAKKVSQQVSKVLYKQNWEDTKD KYLLPPDAPELVQAIKNTAMFSKKL YTEDWEADKTMFYPYNDSPELRRV AQAQKALSDIAYKKGLAEQQTQFTS LPDPPDIEFAKKVTNQVSKQKYKED YENKVKGKWSETPCFEIATARMNSN NISTKKYQEDFEHMKDQIYFMQTET PEYKVNKQAGVAASKVKYKQDYEK NKGKADYNVLPASENPLLRQLKAAG DALSDKLYKENYEKTKAKSINYCET PKFKLDTVLHNFSSDTKYKDSYLKDI LGHYVGSYEDPYHTHCMRVSAQNS DKNYKAEYEEDRGKGFFPQTITQEY EAIKKLDQCKDHTYKVHPDKTKFTQ VTDSPVLMQAQVNSKQLSDLNYKA KHENEKFKCHIPPDTPAFIQHKLNAY NLSDNIYKHDWEKTKAKKFDIKVDA IPLLAAKANTKIASDVMYKKDYEKS KGKMIGALSINDDPKMLHSLKTAKN QSDRLYKENYEKTKAKSMNYCETPK YQLDTLLKNFSEAKYKDSYVQNVLG HYIGSFEDPYQVHCLKISAQNSDKNY KAEYEEDKGKCYFPQTITQEYEAIKK LDQCKDHTYKVHPDKTKFTAVTDTP VLLQAQLNTKQLSDLNYKAKHEGE KFKCHIPADAPQFIQHRVNAYNLSDN IYKHDWEKTKAKKFDIKVDAIPLLA AKANTKIASDVMYKKDYEKSKGKM IGALSINDDPKMLHSLKTAKNQSDHE YRKDYEKSKTIYTAPLDMLPLTHAK KSQAIASDVDYKHLLHNYSYPPDSV NVDLAKKAYALQSDVEYKADYNSW MKGCGWMPFGSLEMEKAKRASDIL NEKKYRQHPDTLKFTSIEDAPIIVQSK INQAQRSDVAYKAKGEEVIHKYSLP ADLPQFIQAKVNAYNISENLYKADL KDLSKKGYDLRIDAIPIKAAKAARQA ASDVQYKKDYEKAKGKMVGFQSLQ DDPKLVHYMNVAKIQSDREYKKAY EKTKTRHNTPHDMVNIVAAKKAQD VASNVNYKHSLHHYTYLPDAMDLE LSKNMMHIQSDNVYKEDYNNWMK GIGWIPIGSLEVEKVKKAGDALNEKK YRQHPDTLKFTSIVDSPVMVQAKQN TQQVSDILYKAKGEDVKHKYTMSPD LPQFLQAKCNAYNLSDVCYKRDWH DLIAKGTNVLGDAIPITAAKASRNIAS DYKYKEAYEKAKGKQVGFRSLQDD PKLVHYMNVAKLQSDREYKKNYEN TKTSYHTPGDMVSITAAKMAQDVAT NVNYKQPIHHYTYLPDALSLEHIRNV NQIQSDNVYKDEYNHFFKGMGWIPI GSLEVEKVKKAGDALNEKKYRQHP DTIKFTSVPDSMGMVLAQHNTKQLS DLNYKVEGEKVKHKYTMDPDVPQFI QAKVNAYNMSDSHYKADWKKTLA KGYDLRPDAIPIVAAKSSRNIASDFK YKEAYEKTKGKQIGFRSLQDDPKLV HFMNVAKMQSDREYKKGYEASKTK YHTPLDMLSVTAAKKSQEVATNTNY KQPFHHYTLLPDALNVEHSRNAMQI QSDNLYKSDFTNWMKGIGWLPLESL EVEKAKKAGEILSEKKYRQHPEKLK FTYAMDTMEQALNKSNKLIMDKRL YTEKWNKDKTTIHVMPDTPDILLSR VNQITMSDKLYKAGWEEEKKKGYD LRPDAISIKAARASRDIASDYKYKQA YEQAKGKQIGFRSLEDDPKLVHFMQ VAKMQSDREYKKAYEKSKTSFQTPV DMLSVVAAKKSQEVATNANYRNVI HTYNMLPDAMSLELAKNMMQIQSD NQYKADYADFMKGIGWLPLGSLEA EKNKKAMEIISEKKYRQHPDTLKYST LMDSMNMVLAKNNAKIMNEHLYK QAWEADKTKVHIMPDIPQIILAKANA INISDKLYKLSLEEAKKKGYDLRTDA IPIKAAKASRDIASDYKYKHSYEKER GKMVGFRSLEDDPKLVHSMQVAKM QSDREYKKNYEKTKTSYHTPADMLS VTAAKDAQANITNTNYKHLIHKYILL PDAMNIQLSKNMNRIQSDNEYKQDY NEWYKGLGWSPAGSLEVEKAKKAT EYASDQKYRQHPSNFQFTKLNDSMD MVLAKQNAHTMNKYLYTVDWNKD KTKIHVMPDTPDILQAKQNQTMYSQ VKYKMGWPSGLERVVNLPSVLIKIF KSKQNKDLIWRFKYKQGYRKQIGHH IGFRSLQDDPKLVLSMNVAKMQSER EYKKDFEKWKTKFSSPVDMLGVVL AKKCQALVSDVDYKNYLHGWTCLP DQNDVIHAKKAYDLQSENLYKSDLE WLKGIGWSPLGSLEAEKNKRASEIIS EKKYRQPPDRNKFTSIPDAMDIVLAK TNAKNRSDILYREAWDKDKTQIHIM PDTPDIILAKANLINTSDKFYRMGYE ELRKKGYDLPVDAIPIKAAKASREIA SEYKYKEGFRMQLGHHIGARNIKDD PKMMWSMHVAKIQSDREYKKDFEK WKTKFSSPVDMLGVVLAKKCQTLV SDIDYKNYLHQWTCLPDQSDVIHAR RAYDLQSDNLYKSDLQWLRGIGWLP SGSLEDEKNKRASQILSDHVYRQHP DQFKFSSLMDSIPMVLAKNNAITMN HRLYTEAWDKDKTTVHIMPDTPEVL LAKQNQINYSEKLYKLGLDEAKRKG YDMRIDAIPIRAAKASRDIASEFKYK EGYRRQLGHHIGARAIHDDPKMMW SMHVAKIQSDREYKKDFEKWKTKFS SPVDMLGVVLAKKCQTLVSDIDYKN YLHQWTCLPDQSDVIHARQAYDLQS DNVYKSDLQWLRGIGWVPIGSLDVE KSKRASEILSDKLYRQPPDKFKFTSV TDSLEQVLAKNNAINMNKRLYTEA WDKDKTQVHIMPDTPEITLARTNKV NYSENLYKLAHEEAKKKGYDLRSD AIPIIAAGLEKDHISDYKYKDGYRKQ LGHHIGARDIKDDPKMMWSMHVAK IQSDREYKKDFEKWKTKFSSPVDML GVVLAKKCQTLVSDIDYKNYLHQW TCLPDQSDVIHARQAYDLQSDNVYK SDLQWLRGIGWVPIGSVDVVKCKRA AEILSDNLYRQPPDTLKFTSVPDSLE QVLAKNNAINMNKRLYTEAWDKDK TQIHIMPDTPEIMLARQNKLNYSETL YKLANEEAKKKGYDLRSDAIPIVAA KASRDIISDYKYKDGYRKQLGHHIG ARDIKDDPKMMWSMHVAKIQSDRE YKKDFEKWKTKFSSPVDMLGVVLA KKCQTLVSDIDYKNYLHQWTCLPDQ SDVIHARRAYDLQSDNIYKSDLQWL RGIGWVPIGSVDVVKCKRAAEILSDN LYRQRPDTLKFTSVPDSLEQVLAKN NAINMNKRLYTEAWDKDKTQIHMM PDTPEITLARQNKINYSENLYRQAME EAKKEGYDLRSDAIPIVAAKASREIA SDYKYKEAYRKQLGHHIGARAIHDD PKMMWSVHVAKMQSDREYKKDFE KYKTRFSSPVDMLGIVLAKKCQTLV SDVDYKHPLHEWTCLPDQNDIIHAR KAYDLQSDNLYKSDLEWLKGIGWV PIGSVEVLKAKRAGEILSDNIYRQRP DTLKFTSVTDSPEQVLAKNNAINMN KRLYTEAWDNDKKTIHVMPDTPEIM LAKLNRINYSDKLYKLALEESRKEG YDLRLDAIPIQAAKASREIASDYKYK EGYRKQLGHHIGARNIKDDPKMMW SIHAGKLQSDLEYKKDFEKWKTKFS SPVDMMGLVQAKKCQILVSDIDYKN LLHEWTCLPDQNDIIQARKAYDLQS DAIYKADLEWLRGIGWVPIDSVGVE HAKRAGEILSERKYRQPAVQLKFTSI TDTPEIVLAKNNALNVSKHLYTEAW DADKTSIHVMPDTPEILLAKSNSANIS HKLYTKGWDESKMKDYDLRADAISI KSAKASRDIASDYKYKEAYEKHKGH HIGARSVEDDPRIMCAMNAGRIQSER EYKKEFQKWKTKFSSPVDMLGILLA KKCQTLVSDIDYRNYLHHWTCLPDQ NDIIQARKAYDLQSDAIYKADLEWL RGIGWMPEGSPEVLRVKNAQHIFRD SVYRTPVVKLKYTSIVDTPEVVLAKS NAENISIPKYREVWDKDKTSIHIMPD TPEINLARTNALNVSNKLYREGWDE MKMSCDVRLDAIPIQAAKASREIASD YKYKLDHEKQKGHYVGTRTARDDN KIRWALIAGKIQNEREYRLHWAKWK SKFQSPPDMLSIEHSKNSQTLVSDIDY RHYLHQWTCMPDQNDVIQARKAYD LQSDAIYKADLEWLRGIGWMPADSV SVNHAKHMADIFNEKKYRTKIETLSF TPVDDRVDYVTAKHSGEILNDIKYR KDWNDTKSKYTLTETPQLHTAQEAA RILDQYLYKESWEKQKATGYILPPD AVPFVHAHHSGDVQSELKYKAEHV KQKGHYVGVPTMRDDPKLVWFEHA GQIQNDRLYKENYHKTKAKIHIPPD MVSVLAAKEGQALASDIDYRNYLH QWICHPDQNDVIQARKAYDLQSDNI YKADLEWLRGIGWIPLDSVDHVRVT RNQEMMNQIKYKKDALANYPNFTS VVDPPEIVLAKINSVNQSDVKYKETF NKLIKGKYIFSPDTPYITHSKDMEKL YSTILYKRAWDGTKAYGYTLDERYI PIVGAKHADFVNSELKYKETYEKLK GHYLAGKEISEFPNVVHCLDFQKMR SLLNYRRHYEDTKANVHIPQDMMN HVLAKRCQYILSDLEYRHYFHQWTS LPEEPNVVRVRHAQEILSDNVYKDD LNWLKGIGCYVWDTPQILHAKKSYD LQSQILYTAAGKENLKNYNLVTDTP LYVTALQSGINASEVKYKENYHQTK DKYTTVLETVDYDRIKNLKDLFSSNL YKEAWDKVKATSYILPPNTVSLTHA KNQKYMASHIKYREEYEKFKALYTL PRSVEDDPNTARCLRVGKFNIDRLYR SVYEKNKMKIHIVPDMVEMVTAKDS QKKVSEIDYRLHLHEWICHPDLQVN SHVRKVTDQISDIVYKDDLTWLKGI GCYVWDTPEILHAKHAYDLRNDIKY KAHVLKTRNNYKLVTDTPVYVQAV KSGKQLSDAVYHYDYVHSIRGRVAP TTKTVDLDRALHAYKLQSENLYRKA GLHALPTGYRLPVDTPHFKHTKDTR YMSSYFKYKEVYEHMKAYGYTLGP NDVPFVNVRRVNNITSERLYRQLYH KLKDKIHTTPDTPEIRQVKKTQEAVS ELIYKSDFFKMQGHMISLPYTPQVLH CRYVGDITSDIKYKEDLQVLRGMGC FLYDTPDMVRSRHLRKLWSHYLYTD KARKMRDKYKVVLDTPEYRKVQEL KTHLSELVYRAAGRKQKSIFTSVPDT PDLTRAKRGQKLQSQYLYVELATKE RPHHHAGNQTTALKHARHVKDMVS ENKYKIQYEKMKDKYTPVPDTPILIR AKRAYWNASDLRYKETFQKTKGKY HTVKDALDIVYHRTVTDHISKIKYKE NYMSQLGIWRSIPDRPEHFHHRAVT DAVSDVKYKQDLTWLKGIGCYAYD TPDFTLAEKNKTLYSKYKYKEVFER TKSNFKYVADCPINRHFKFATQLMN EKKYRADYEQRKDKYHLVVDEPRH LLAKIAGDQISQIKYRKNYEETKDKF TSIVDTPEHLRTTKVNKQISDILYKLE YNKAKPRGYTTIHDTPMLLHVRKVK DEVSDLKYKEVYQRTKSNCTIEPDA VHIKAAKDAYKVNTNLDYKKKYEA TKAYWKWTPDRPDFIQAAKSTLQQS DFEYKLDREYLKGCKLSVTDDKDM VLALKNSIIESDLKYKEKHVKERGSC HAVPDTPQILLAKTVSSLVSENKYKS YVKKHLAQGSYTTLPETRDTIHVKE VTKNVSDTNYKKKFVKEKGKSNYSI MLEPPDVKHAMDVAKKQSNVAYKK DAKENLHYTTVADRPDIKKATQAAK QASEVEYRAKHRKEGSHGLSMLGRP DIEMAKKAAKLSSQVQYRENFNKEK GKTPKYNPKDSQLYKVMKDANTLA SEVKYKADLKKLHKPVTDMKESLIM NHVLNTSHLASSYQYKKNYEKSKGH YHTIPDNLEQLHLKEATELQSIVKYK EKYEKERGKPMLDFETPTYITAKESQ QMQSGKEYRKDYEESIKGRNLTGLE VTPALLHVKYATKIASEKEYRKDLE ESIRGKGLSEMEDTPDMLRAKNATQI LNEKEYKRDLELEVKGRGLNAMAN ETPDFLRARNATDIASQIKYKQSAEM EKANFTSVVDTPEIIHAQQVKNLSSQ KKYKEDAEKCMSYYETVLDTPEMQ RVRENQKNFSLLQYQYDLKNSKGKI TVVQDTPEILRVKENQKNFSSVLYKE DVSPGTAIGKTPEMMRVKQTQDHIS SVKYKEVIGQGTPIPDLPEVKRVKQT QKHISSVMYKENLGTGIPTPVTPEIER VKRNQENFSSVLYKENLGKGTPTPIT PEMERVKRNQENFSSILYKENLSKGT PLPVTPEMERVKRNQENFSSVLYKE NVGKGTPTPVTPEMQRVKRNQENIS SVLYKENLGKATPTPFTPEMERVKR NQENFSSVLYKENMRKATPTPVTPE MERAKRNQENISSVLYSDSFRKQIQG KAAYVLDTPEMRRVRETQRHISTVK YHEDFEKHKGCFTPVVTDPITERVKK NTQDFSDICYRGIQRKVVEMEQKRN DQDQETITGLRVWRTNPGSVFDYDP AEDNIQSRSLHMINAQAQRRSREQSR SASGLSISGGEEKSEHSEAAHLSTYS DGGVFFSAASTGYKHARTTELPQQR SSSVATQQTTVSSIPSHPSTAGKIFRA MYDYMAADADEVSFKDGDAIVNVQ AIDEGWMYGTVQRTGRTGM LPANYVEAI SEQ ID F1MPU4 Myotilin/Bos MFNYERPKHFIQSQNPCGSRLQPPGP NO: 94 (UniProtKB) taurus EISSYSSQTKQSSITIQPRQCTEQRYS ASSTVSSHITMSSSAFPASPQQLAGSN PAQRVTATYNQSPASFLSSILPSQPDY SSSKNPSTVDSNYQQPSVGQPINVKS SQNANAKLTPKTPDHEIQGSKEALIQ DLERKLKCKDSLLHNGNQRLTYEEK MARRLLGPQNAAAVFQGQNDSEAQ DSAQQHNIEHARLQVPTSQVRSRSSS RGDVNDQDAIQEKFYPPRFIQVPEN MSIEEGRFCRMDFKVSGLPAPDVSW YLNGRPVQSDDFHKMIVSEKGFHSLI FEVVRASDAGAYACVAKNRAGEAT FTVQLDVLAKEHRRAPMFIYKPQSK KVFEGESVKLECQISAVPPPKLFWKR NNEMVQFNTDRISLYHDNSGRVTLLI KDVNKKDAGWYTVSAVNEAGVTTC NTRLDVTARPNQTLPAPKQLRVRPTF SKYLALNGKGLNVKQAFNPEGEFQR LAAQSGLYESEEL SEQ ID A0A1D5NT92 Myotilin MSVRNLPSVSSMCQPTMFNYERPKH NO: 95 (UniProtKB) OS = Gallus FIQSKNVCQGQQQPPGSSTSTESSRQI gallus KQSSILIQPRNPSGQKFSSSSSLSSSITL SSPSCSAPKESTYPVTPASAQSPASSS SGQRLISMPNQTPAAFLCSVLPSQPD YNSQTPPMEPHYSKPMYKKQASINS MQKTSDQEIRGTKEALIQDLEKKLRC KDNLLQNGNQRLTYEERMARRLLGP ENAASVLEAQSEDMQNTQNAENVR LQVPTTHVRSRPSSRGDERGHDSIQE KFFQPRFTQVPEDVIIEEGRFCRLDFK VSGLPTPDVMWYQNGRMVHQDQFH KMIVSEKGFHSFIFEAVKSSDAGTYE CVAVNRAGESSFAVKVEVIAKEHHT PPTFIFKPQSKKVFEGDTARLECQISA IPTPRIYWKRNNEMVQYNTDRISLLH DNTGRICLLIHNANKKDAGWYTVSA VNGAGVATCHARLEVATHTNKPVP APKQLRVRPTFSKYLALNGRGLDVK QAFSPEGEFQRLAEQSGLYESDEL SEQ ID F1RH92 Myotilin/Sus MFNYERPKHFIQSQNPCGSRLQPPGP NO: 96 (UniProtKB) scrofa ETSSYSSQTKQSSIIIQPRQCTEQRFSA SSTVSSHITMSSSAFPASPQQPAASNP GQRVTATYNQSPASFLSSILPSQPDYS SSKIPSTMDSNYQQPSVGQPVNAKPS QSLNAKPIPRTPDHEIQGSKEALIQDL ERKLKCKDSLLHNGNQRLTYEEKM ARRLLGPQNAAAVFQAQNDSAAQD SPQQHNSEHARLQVPTSQVRSRSSSR GDVNDQDAIQEKFYPPRFIQVPENMS VEEGRFCRMDFKVSGLPAPDVSWYL NGRPVQSDDLHKMIVSEKGFHSLIFE VVRASDAGAYACVAKNRAGEATFT VQLDVLAKEHRRAPMFIYKPQSKKV FEGDSVKLECQISAIPPPKLFWKRNN EMVQFNTDRISLYHDNSGRVTLLIKD VNKKDAGWYTVSAVNEAGVITCNT RLDVTARPNQTLPAPKQLRVRPTFSK YLALNGRGLDVKQAFNPEGEFQRLA AQSGLYESEEL SEQ ID XP_013837221.1 Twinfilin-2/Sus MAHQTGIHATEELKEFFAKARAGSV NO: 97 (NCBI) scrofa RLIKVVIEDEQLVLGASRELMGCWD QDYDRAVLPLLDAQQPCYLLYRLDT QNAQGFEWLFLAWSPDNSPVRLKM LYAATRATVKKEFGGGHIKDELFGT VKDDLSFAGYQKHLSSCAAPAPLTS AERELQQIRINEVKTEISVESKHQTLQ GLAFPLQPQAQRALQQLRQKMINYI QLKLDLERETIELVHTEPTDVAQLPS RVPRDAARYHFFLYKHTHEGDPLES VVFIYSMPGYKCSIKERMLYSSCKSR LLDSVEQDFQLEIAKKIEIGDGAELT AEFLYDEVHPKQHAFKQAFAKPKGP GGKRGHKRLIRGPGENGDDS SEQ ID XP_024844129.1 Dystrophin MLWWEEVEDCYEREDVQKKTFTK NO: 98 (NCBI) isoform X4/Bos WINAQFSKFGKQHIENLFSDLQDGRR taurus LLDLLEGLTGQKLPKEKGSTRVHAL NNVNKALQVLQKNNVDLVNIGSSDI VDGNHKLTLGLIWNIILHWQVKNVM KNIMAGLQQTNSEKILLSWVRQSTR NYPQVNVINFTTSWSDGLALNALIHS HRPDLFDWNSVVRQQSATQRLEHAF NIAKYQLGIEKLLDPEDVATTYPDKK SILMYVTSLFQVLPQQVSLEAIQEVE MLPRPSKVTREEHFQLHHQMHYSQQ ITVSLAQGYERTPSSPQPRFKSYAYT QAAYVSTSDPTRSPFPSQRLEAPEDK SFGSPLMETEVNLDSYQTALEEVLS WLLSAEDTLQAQGEISNDVEEVKEQ FHTHEGYMMDLTSHQGRVGNVLQL GSQLIGSGKLSEDEETEVQEQMNLLN SRWECLRVASMEKQSNLHKVLMDL QNQQLKELNAWLTKTEERTRKMEK EPLGPDLEDLKRQIQQHKVLQEDLE QEQVRVNSLTHMVVVVDESSGDHA TAALEEQLKVLGDRWANICRWTED RWVLLQDVLLKWQRFTEEQCLFST WLSDKEDALNKIPTSGFKDQSEMLSS LQKLAVLKTDLEKKKQTMDKLCSL NHDLLSTLKNTLVAQKMEAWLDNF AQRWDNLVQKLEKSSTQISQAVTTT QPSLTQTTVMETVTMVTTREQILVK HAQEELPPPPPQKKRQIIVDSEIKKRL DVDITELHSWITRSEAVLQSPEFAVY RKEGNFSDLKEKVNAIEREKAEKFR KLQDASRSAQALVEQMVNEGVNAD SIKQASEQLNSRWIEFCQLLSERLNW LEYQNNIITFYNQLQQLEQMTTTAEN WLKTQPTTPSEPTAVKSQLKNCKDE VNRLSALQPQIERLKIQSIALKEKGQ GPMFLDADFVAFTNHFNQVFADMQ AREKELQTILDTLPTVRYQETMSTIL TWIQQSETKLSIPQVTVTEYEIMEQR LEELQALQSSLQEHQNDLNYLSTTV KEMSRKAPSHISQRYQSEFENIEGRW KKLSAQLNERCQKLEEQMAKLRKL QNHIKTLKKWMAEVDVFLKDEWPA LGDSEILKKQLKQCRLLVSDIQTIQPS LNSVNEGGQKIKTEAEPEFASRLETE LRELNTQWDYICRQVYARKEALKG GLDKTVSLQKDLSEMHEWMTQAEE EYLERDFEYKTPDELQTAVEEMKRA KEEAQQKEAKVQLLTESVNSVIAQA PPAAQEALRKELDTLTTNYQWLCTR LNGKCKTLEEVWACWHELLSYLEK ANKWLNEVELKLKTTENIPGGAEEIS EVLSSLENLMQHSEDNPNQIRILAQT LTDGGVMDELINEELETFNSRWREL HEEAVRRQKLLEQSIQSAQEIEKSLQ LIQESLSSIDKQLAAYIADKVDAAQM PQEAQKIQSDLTSHEISLEEMKKHYQ GKETAQRVLSQIEVAQKKMQDVSM KFRLFQKPANFEQRLQESKMILDEVK MHLPALETKSVEQEVVQSQLNHCVN LYKSLSEVKSEVEMVIKTGRQIVQKK QTENPKELDERVTALKLHYNELGAK VTERKQQLEKCLKLSRKMRKEMNA LTEWLAATDLELTKRSAVEGMPSNL DSEVAWGKATQKETEKQKVHLKSIT ELGEALKTVLGKKETLVEDKLSLLN SNWIAVTSRAEEWLNLLLEYQKHME TFDQNVDHITKWIIQADALLDESEKK KPQQKEDMLKRLKAELNDIRPKVDS TRDQAANLMANRGDHCRKVIEPKIS ELNHRFAAISHRIKTGKASIPLKELEQ FNSDIQKLLEPLEAEIQQGVNLKEED FNKDMSEDNEGTVKELLQRGDNLQ QRITDERKREEIKIKQQLLQTKHNAL KDLRSQRRKKALEISHQWYQYKRQ ADDLLKCLDDIEKKLASLPEPRDERK IKEIDRELQKKKEELNAVHRQAEGLS EDGAAMAVESTQIQLSKRWREIESKF AQFRRLNFAQIHTVHEESVMVMTED MPLEISYVPSTYLTEITHVLQALSEVE QLLNAPDLCAKDFQDLFKQEESLKNI KDNLQQISGRIDVIHNKKAAALQSTT PPEKARLQEALSRLDFQWERVNKMY KDRQGQFDRSVEKWRRFHYDMKIF NQWLTEAEHFLKKTQIPENWEHAKY KWYLKELQDGIGQRQTVVRVLNAT GEEIIQQSSKIDASILQEKLGSLNLRW QEVCKQLAERKKRLEEQKNILSEFQR DLNEFVLWLEEAGNISSIPLEPGNEQ QLKEKLEEVKLLAEELPLRQGILKQL NETGGTVLVSAPISPEEQDKLENKLK QTNLQWIKVSRSLPEKQGEIEAHIKD LGQLEEQLNHLLLWLSPIRSQLEIYN QPNQTGPFDIKETEVAVQAKQPDVE GILSKGQNLYKEKPATEPVKRKLEDL SSEWKAVTHLLQELRAKWPGPVPGL TATGAPPSQTVALVTQPVVAKETAV SKLEMPSSLLLEVPALADFNRAWTE LTDWLSLLDRVLKAQRVMVGDLEDI NEMIIKQKATLQDLEQRRPQLEELIT AAQNLKNKTSNQEARTIITDRIERIQS QWDEVQEHLQNRRQQLNEMLKDST QWLEAKEEAEQVVGQARAKLETWK EGPYTMDAIQRKITETKQLAKDLRQ WQINVDVANDLALKLLRDYSADDT RKVHMITENINASWASIHKRVSERET ALEETHRLLQQFPLDLEKFLAWLTE AETTANVLQDATHKERLLEDSKGVR ELMKQWQDLQGEIEAHTDIYHNLDE NGQKILRSLEGSDDAVLLQRRLDNM NFKWSELRKKSLNIRSHLEASSDQW KRLHLSLQELLVWLQLKDDELSRQA PIGGDFPAVQKQNDIHRAFKRELKTK EPVIMSTLETVRIFLTEQPLEGLEKLY QEPRELPPEEKAQNVTRLLRKQAEE VNTEWEKLNLHSADWQRKIDEALER LQELQEATDELDLKLRQAEVIKGSW QPVGDLLIDSLQDHLEKVKALRGEIA PLKENVSHVSDLARQLTTLGIQLSPY NLSTLEDLNTRWKLLQVAVEDRIRQ LHEAHRDFGPASQHFLSTSVQGPWE RAISPNKVPYYINHETQTTCWDHPK MTELYQSLADLNNVRFSAYRTAMK LRRLQKALCLDLLSLSAACDALDQH NLKQNDQPMDILQIINCLTTIYDRLE QEHNNLVNVPLCVDMCLNWLLNVY DTGRTGRIRVLSFKTGIISLCKAHLED KYRYLFKQVASSTGFCDQRRLGLLL HDSIQIPRQLGEVASFGGSNIEPSVRS CFQFANNKPEIEAALFLDWMRLEPQ SMVWLPVLHRVAAAETAKHQAKCN ICKECPIIGFRYRSLKHFNYDICQSCFF SGRVAKGHKMHYPMVEYCTPTTSG EDVRDFAKVLKNKFRTKRYFAKHPR MGYLPVQTVLEGDNMETPVTLINFW PVDSAPASSPQLSHDDTHSRIEHYAS RLAEMENSSGSYLNDSISPNESIDDE HLLIQHYCQSLNQDSPLSQPRSPAQIL ISLESEERGELERILADLEEENRNLQA EYDRLKEQHEHKGLSPLPSPPEMMP TSPQSPRDAELIAEAKLLRQHKGRLE ARMQILEDHNKQLESQLHRLRQLLE QPQAEAKVNGTTVSSPSTSLQRSDSS QPMLLRVVGSQTSESMGEEDLLSPP QDSSTGLEEVMEQLNNSFPSSRGRNT PGKPMREDTM SEQ ID Q05JF3 Calsequestrin/ MSAADRMGARAVPGLRLALLLLMV NO: 99 (UniProtKB) Bos taurus LGTPKSGVQGEEGLDFPEYDGVDRV VNVNAKNYKNVFKKYEVLALLYHE PPEDDKASQRQFEMDELILELAAQVL EDKGVGFGMVDSEKDAAVAKKLGL TEEDSVYVFKGDEVIEYDGEFSADTL VEFLLDVLEDPVELIEGERELQAFENI EDDNKLIGYFKNKDSEHYKAYEDAA EEFHPYIPFFATFDSKVAKKLTLKLN EIDFYEAFMEEPVTIPDKPNSEEEIVS FVEAHKRSTLRKLKPESMYETWEDD LDGIHIVAFAEETDPDGYEFLETLKA VAQDNTDNPDLSIIWIDPDDFPLLVP YWEKTFNIDLSAPQIGVVNVTDADS VWMEMDDEEDLPSAEELEDWLEDV LEGEINTEDDDEEDD Cardiac muscle tissue SEQ ID P68034 Actin, alpha MCDDEETTALVCDNGSGLVKAGFA NO: 100 (UniProtKB) cardiac muscle GDDAPRAVFPSIVGRPRHQGVMVG 1/Gallus gallus MGQKDSYVGDEAQSKRGILTLKYPI EHGIITNWDDMEKIWHHTFYNELRV APEEHPTLLTEAPLNPKANREKMTQI MFETFNVPAMYVAIQAVLSLYASGR TTGIVLDSGDGVTHNVPIYEGYALPH AIMRLDLAGRDLTDYLMKILTERGY SFVTTAEREIVRDIKEKLCYVALDFE NEMATAASSSSEEKSYELPDGQVITI GNERFRCPETLFQPSFIGMESAGIHET TYNSIMKCDIDIRKDLYANNVLSGGT TMYPGIADRMQKEITALAPSTMKIKII APPERKYSVWIGGSILASLSTFQQMW ISKQEYDEAGPSIVHRKCF SEQ ID XP_004938861.1 Gamma- MVREQYTTTTPGTSLERPKNEYVYKI NO: 101 (NCBI) sarcoglycan GIYGWRKRCLYLFVLLLLIILVVNFS isoform LTIWILKVMWFSPTGMGHLRVTKEG X2/Gallus gallus LRLEGESEFLFPLYAKEIHSRVDSSLL LQSTHNVTVNARNSNGEVTGRLNVG PKMVEFHGQQFQINSKDGKPLFTVD ENEVVIGTDKLRVTGPEGALFEHSVE TPLVKAEAFKQLRLESPTRSLSMDAP RGIN IKAQAGNIEALSQMDIKLHSSDGVLL LDAETVRLPKLPEGTRGGPGVSQGL YEICVCPDGKLYLSVAGLGSTCQEYS RVCQ SEQ ID NP_990097.1 Myosin heavy MMDMTEFGEAAPFLRKSEKELMML NO: 102 (NCBI) chain, cardiac QTVAFDGKKKCWVPDDKKAYVEAE muscle ITESSGGKVTVETTDGRTMTIKEDDV isoform/Gallus QSMNPPKFDMIEDMAMLTHLNEASV gallus LYNLRKRYSNWMIYTYSGLFCVTIN PYKWLPVYKSEVVAAYKGKRRSEA PPHIFSIADNAYHDMLRNRENQSMLI TGESGAGKTVNTKRVIQYFATVAAL GEPGKKSQPATKTGGTLEDQIIQANP ALEAFGNAKTLRNDNSSRFGKFIRIH FGTTGKLSSADIEIYLLEKSRVIFQQP GERDYHIFYQILSGKKPELLDMLLVS TNPYDYHFCSQGVVTVDNLDDGEEL MATDQAMDILGFVPDEKYGAYKLT GAIMHFGNMKFKQRPREEQAEADGT ESADKAAYLMGINSSDLVKGLLHPR VKVGNEYVTKGQSVEQVLYAVGAL SKAVYDRMFKWLVVRINKTLDTKLP RQFFIGVLDIAGFEIFDFNSFEQLCINY TNEKLQQFFNHHMFVLEQEEYKKEG IEWVFIDFGMDLQACIDLIEKPLGILSI LEEECMFPKATDMTFKAKLYDNHLG KSPNLQKPRPDKKRKYEAHFELIHY AGSVPYNIIGWLEKNKDPLNETVVGI FQKSSNKLLASLFESYVGADSADQG GEKKRKKGASFQTVSSLHKENLNKL MTNLRSTAPHFVRCIIPNESKTPGEM DAFLVLHQLRCNGVLEGIRICRKGFP NRVLYADFKQRYRILNPGAIPEDKFV DSRKAAEKLLASLDIDHNQYRFGHT KVFFKAGLLGHLEEMRDERLAKILT MIQARARGRLMRIEFQKIVERRDALL VIQWNIRAFMAVKNWPWMKLFFKI KPLLKSAETEKEMANMKEEFLKLKE ALEKSEARRKELEEKQVSLVQEKND LLLQLQAEQDTLADAEERCDLLIKSK IQLEAKVKELTERVEDEEEMNSELTS KKRKLEDECSELKKDIDDLEITLAKV EKEKHATENKVKNLTEEMATLDENI SKLTKEKKSLQEAHQQVLDDLQAEE DKVNTLSKAKVKLEQQVDDLEGSLE QEKKVRMDLERAKRKLEGDLKLTQ ESVMDLENDKLQMEEKLKKKEFEM SQLNSKIEDEQAIVMQLQKKIKELQA RIEELEEELEAERAARAKVEKQRSDL ARELEVLSERLEEAGGATAAQLEMN KKREAEFLKLARDLEEATLHYEATA AALRKKHADSVAEMGEQLDNLQRV KQKLEKEKSELKMEVDDLTSNMEQT VKGKANAEKLCRTYEDHLNETKTKL DEMTRLMNDLTTQKTKLQSENGEFV RQLEEKESLISQLSRGKTSFTQQIEEL RRQLEEETKSKNALAHALQAARHDC DLLREQYEEEQEAKAELQRALSKGN AEVAQWRTKYETDAIQRTEELEDAK KKLAARLQEAEEAIEAANAKCSSLE KTKHRLQNELEDMMIDLEKANSAA ASLDKKQRGFDKIINDWKQKYEESQ AELEASQKEARSLSTELFKLKNAYEE TLDHLETLKRENKNLQEEISDLTNQI SEGNKNLHEIEKVKKQVEQEKSEVQ LALEEAEGALEHEESKTLRFQLELSQ LKADFERKLAEKDEEMENIRRNQQR TIDSLQSTLDSEARSRNEAIRLKKKM EGDLNEMEIQLSHANRHAAEATKSA RGLQTQIKELQVQLDDLGHLNEDLK EQLAVSDRRNNLLQSELDELRALLD QTERARKLAEHELLEATERVNLLHT QNTSLINQKKKLEGDISQMQNEVEES IQECRNAEEKAKKAITDAAMMAEEL KKEQDTSAHLERMKKNMEQTIKDL QKRLDEAEQIALKGGKKQIQKLESR VRELENELENELRRNSDAQKGARKF ERRIKEVTYQSEEDKKNLARMQDLI DKLQLKVKSYKHQAEEAEAQANLY LSKYRKQQHDLDDAEERAEIAESQV NKLRSKSRDIGMKKVHEEE SEQ ID Q90688 Myosin-binding MPEPAKKAVSAFTKKPKTTEVAAGS NO: 103 (UniProtKB) protein C, TAVFEAETEKTGIKVKWQRAGTEIT cardiac- DSEKYAIKAEGNKHSLTISNVGKDDE type/Gallus VTYAVIAGTSKVKFELKVKEPEKSEP gallus VAPAEASPAPAASELPAPPVESNQNP EVPPAETQPEEPVDPIGLFVTRPQDG EVTVGGNITFTAKVAGESLLKKPSVK WFKGKWMDLASKVGKHLQLHDNY DRNNKVYTFEMEIIEANMTFAGGYR CEVSTKDKFDSSNFNLIVNEAPVSGE MDIRAAFRRTSLAGGGRRMTSAFLS TEGLEESGELNFSALLKKRDSFLRTA NRGDGKSDSQPDVDVWEILRKAPPS EYEKIAFQYGITDLRGMLKRLKRIKK EEKKSTAFLKKLDPAYQVDKGQKIK LMVEVANPDADVKWLKNGQEIQVS GSKYIFEAIGNKRILTINHCSLADDAA YECVVAEEKSFTELFVKEPPILITHPL EDQMVMVGERVEFECEVSEEGATV KWEKDGVELTREETFKYRFKKDGK KQYLIINESTKEDSGHYTVKTNGGVS VAELIVQEKKLEVYQSIADLTVKAR DQAVFKCEVSDENVKGIWLKNGKE VVPDERIKISHIGRIHKLTIEDVTPGD EADYSFIPQGFAYNLSAKLQFLEVKI DFVPREEPPKIHLDCLGQSPDTIVVV AGNKLRLDVPISGDPTPTVIWQKVN KKGELVHQSNEDSLTPSENSSDLSTD SKLLFESEGRVRVEKHEDHCVFIIEG AEKEDEGVYRVIVKNPVGEDKADIT VKVIDVPDPPEAPKISNIGEDYCTVQ WQPPTYDGGQPVLGYILERKKKKSY RWMRLNFDLLKELTYEAKRMIEGV VYEMRIYAVNSIGMSRPSPASQPFMP IAPPSEPTHFTVEDVSDTTVALKWRP PERIGAGGLDGYIVEYCKDGSAEWT PALPGLTERTSALIKDLVTGDKLYFR VKAINLAGESGAAIIKEPVTVQEIMQ RPKICVPRHLRQTLVKKVGETINIMIP FQGKPRPKISWMKDGQTLDSKDVGI RNSSTDTILFIRKAELHHSGAYEVTL QIENMTDTVAITIQIIDKPGPPQNIKL ADVWGFNVALEWTPPQDDGNAQIL GYTVQKADKKTMEWYTVYDHYRR TNCVVSDLIMGNEYFFRVFSENLCGL SETAATTKNPAYIQKTGTTYKPPSYK EHDFSEPPKFTHPLVNRSVIAGYNTT LSCAVRGIPKPKIFWYKNKVDLSGD AKYRMFSKQGVLTLEIRKPTPLDGGF YTCKAVNERGEAEIECRLDVRVPQ SEQ ID F1NBZ9 Myosin-binding MPEPAKKAVSAFTKKPKTTEVAAGS NO: 104 (UniProtKB) protein C, TAVFEAETEKTGIKVKWQRAGTEIT cardiac- DSEKYAIKAEGNKHSLTISNVGKDDE type/Gallus VTYAVIAGTSKVKFELKVKEPEKSEP gallus VAPAEASAPAASELPAPPVESNQNPE VPPAETQPEEPVDPIGLFVTRPQDGE VTVGGNITFTAKVAGESLLKKPSVK WFKGKWMDLASKVGKHLQLHDNY DRNNKVYTFEMEIIEANMTFAGGYR CEVSTKDKFDSSNFNLIVNEAPVSGE MDIRAAFRRTSLAGGGRRMTSAFLS TEGLEESGELNFSALLKKSSSFLRTA NRGDGKSDSQPDVDVWEILRKAPPS EYEKIAFQYGITDLRGMLKRLKRIKK EEKKSTAFLKKLDPAYQVDKGQKIK LMVEVANPDADVKWLKNGQEIQVS GSRYIFEAIGNKRILTINHCSLADDAA YECVVAEEKSFTELFVKEPPILITHPL EDQMVMVGERVEFECEVSEEGATV KWEKDGVELTREETFKYRFKKDGK KQYLIINESTKEDSGHYTVKTNGGVS VAELIVQEKKLEVYQSIADLTVKAR DQAVFKCEVSDENVKGIWLKNGKE VVPDERIKISHIGRIHKLTIEDVTPGD EADYSFIPQGFAYNLSAKLQFLEVKI DFVPREEPPKIHLDCLGQSPDTIVVV AGNKLRLDVPISGDPTPTVIWQKVN KKGELVHQSNEDSLTPSENSSDLSTD SKLLFESEGRVRVEKHEDHCVFIIEG AEKEDEGVYRVIVKNPVGEDKADIT VKVIDVPDPPEAPKISNIGEDYCTVQ WQPPTYDGGQPVLGYILERKKKKSY RWMRLNFDLLKELTYEAKRMIEGV VYEMRIYAVNSIGMSRPSPASQPFMP IAPPSEPTHFTVEDVSDTTVALKWRP PERIGAGGLDGYIVEYCKDGSAEWT PALPGLTERTSALIKDLVTGDKLYFR VKAINLAGESGAAIIKEPVTVQEIMQ RPKIWLPRHLRQTLVKKVGETINIMI PFQGKPRPKISWMKDGQTLDSKDVG IRNSSTDTILFIRKAELHHSGAYEVTL QIENMTDTVAITIQIIDKPGPPQNIKL ADVWGFNVALEWTPPQDDGNAQIL GYTVQKADKKTMEWYTVYDHYRR TNCVVSDLIMGNEYFFRVFSENLCGL SETAATTKNPAYIQKTGTTYKPPSYK EHDFSEPPKFTHPLVNRSVIAGYNTT LSCAVRGIPKPKIFWYKNKVDLSGD AKYRMFSKQGVLTLEIRKPTPFDGGF YTCKAVNERGEAEIECRLDVRVPQ SEQ ID NP_998735.1 Troponin 1, MAEEEEPKPPPLRRKSSANYRGYAV NO: 105 (NCBI) cardiac EPHAKRQSKISASRKLQLKTLLLQRA muscle/Gallus KRELEREEQERAGEKQRHLGELCPPP gallus ELEGLGVAQLQELCRELHARIGRVD EERYDMGTRVSKNMAEMEELRRRV AGGRFVRPALRRVRLSADAMMAAL LGSKHRVGTDLRAGLRQVRKDDAE KESREVGDWRKNVDALSGMEGRKK KFEAPGGGQG SEQ ID A0A1D5PF28 Troponin T, MKQKHQEKGESKPKPKPFMPNLVPP NO: 106 (UniProtKB) cardiac muscle KIPDGERLDFDDIHRKRMEKDLNEL isoforms/Gallus QALIEAHFESRKKEEEELISLKDRIEQ gallus RRAERAEQQRIRSEREKERQARMAE ERARKEEEEARKKAEEEARKKKAFS NMLHFGGYMQKSEKKGGKKQTERE KKKKILSERRKPLNIDHLSEDKLRDK AKELWQTIRDLEAEKFDLQEKFKRQ KYEINVLRNRVSDHQKV SEQ ID F1RRT2 Myosin light MPPKKPEPKKEAAKAAPAPAPAPAP NO: 107 (UniProtKB) chain 4/Sus APAPPPEPAKEPTFDPKSIKIDFTADQI scrofa EEFKEAFSLFDRTPTGEMKITYGQCG DVLRALGQNPTNAEVLRVLGKPKPE EMNAKMLDFETFLPILQHISRNKEQG TYEDFVEGLRVFDKESNGTVMGAEL RHVLATLGEKMTEAEVEQLLAGQED ANGCINYEAFVKHIMSG SEQ ID Q910C5 Atrial myosin MEALLGAAAPFLRAPEGPRPTPAGD NO: 108 (UniProtKB) heavy TRGLCFVPHPQLEFIRARVTARAGNG chain/Gallus VTVTTEMGETLTVPEADVHPQNPPK gallus FDRIEDMAMLTFLHEPAVLYNLKER YASWMIYTYSGLFCVTVNPYKWLPV YNAEVVAAYRGKKRTEVPPHIFSISD NAYQNMLTDRENQSILITGESGAGK TVNTKRVIQYFASIAAIGHRKKEVAN SSKGTLEDQIIQANPALEAFGNAKTV RNDNSSRFGKFIRIHFGATGKLASADI ETYLLEKSRVIFQLKAERNYHIFYQIL SNKKPELLEMLLITNNPYDYSYVSQG EVTVASIDDSEELLATDSAFDVLGFT AEEKAGVYKLTGAIMHFGNMKFKQ KQREEQAEPDGTEDCDKSAYLMGL NSADLLKGLCHPRVKVGNEYVTKG QSVQQVYYSIGALAKAVYEKMFNW MVVRINNSLETKQPRQYFIGVLDIAG FEIFDFNSFEQLCINFTNEKLQQFFNH HMFVLEQEEYKKEGIEWEFIDFGMD LQACIDLIEKPMGIMSILEEECMFPKA SDMTFKAKLFDNHLGKSANFGKPRN VKGKSEAHFSLIHYAGTVDYNIIGWL EKNKDPLNETVVGLYQKSALKLLAS LFSNYAGADAGGDGGKGKGAKKKG SSFQTVSALHRENLNKLMANLKTTH PHFVRCLIPNERKEPGVMDNPLVMH QLRCNGVLEGIRICRKGFPNRILYGD FRQRYRIPNPTAIPEGQFIDSRKGAEK LLGSLDIDHNQYKFGHTKVFFKAGL LGLLEEMRDERLSLIITRIQAQARGQ LMRIEFKKILERRDALLVIQWNIRAF MGVKNWPWMKLYFKIKPLLKSAET EKEMQTMKEEFGHLKEALEKSAARR KELEEKMVSMLQEKNDLQLQVQAE QDNLADAEERCDQLIKNKIQLEAKV KEMTERLEEEEEMNAELAAKKRKLE DECSELKKDIDDLELSLAKVEKEKH ATENKVKNLTEEMAGLDENITKLTK EKKILQESHQQALDDLQAEEDKVNT LAKAKGKLEQQVDDLESSLEQEKKI RMDLERAKRKLEGDLKLAQESIMDL ENDKQQLEERLKKKDFELNTLNARI EDEQAISAQLQKKLKELQARIEELEE ELEAERTGRAKVEKLRSELLQELEET SERLEEAGGATSVQLELNKKREAEF QKLRRDLEEATLQHEATAATLRKKH ADSVAELSEQLDNLQRVKQKLEKEK SELKLELDDVNSNTEQLIKAKTNLEK MCRTTEDQMNEHRSKLEEAQRTVT DLSTQRAKLQTENSELSRQLEEKEAF INQLTRGKLTYTQQLEDLKRQLEEEA KAKNALAHALQSAQHDCDLLREQY EEEMEAKAELQRALSKANSEVAQW RTKYETDAIQRTEELEEAKKKLAQR LQEAEEAVEAVNAKCSSLEKTKHRL QNEIEDLMADVERSNAAAAALDKK QRNFDKILSEWKQKFEESQTELEASQ KEARSLSTELFKLKNAYEESLEHLET FKRENKNLQEEILDLTEQLGASQKSI HELEKVRKQLDAEKLELQAALEEAE ASLEHEEGKILRAQLEFNQVKADYE RKLAEKDEEIEQSKRNHLRVVDSLQ TSLDAETRSRNEALRLKKKMEGDLN EMEIQLSHANRTAAEAQKQVKALQG YLKDTQLQLDDVVRANEDLKENIAI VERRNNLLQSELEELRAMVEQSERA RKLAEQELIEASERVQLLHSQNTSLI NQKKKMEADISQLQTEVEEAIQECR NAEEKAKKAITDAAMMAEELKKEQ DTSAHLERMKKNMEQTVKDLQLRL DEAEQLALKGGKKQLQKLEVRVREL ENELEAEQKRNAESIKGLRKSERRVK ELSYQTEEDRKNMVRLQDLVDKLQL KVKAYKRQAEEAEEQANSNLAKFR KVQHELDEAEERADMAESQVNKLR ARSRDIGAKKGLNEE SEQ ID Q8UWA0 Myosin heavy MSMLDMSEFGEAAEYLRKSYTEQLK NO: 109 (UniProtKB) chain/Gallus LQTIPFDGKKRAWIPDEKEAYIEVEIK gallus ESTGGKVTVETKDKQTRVVKEDELQ AMNPPKFDMIEDMAMLTHLNEASV LYNLKRRYSHWMIYTYSGLFCVTIN PYKWLPVYTAPVVAAYKGKRRSEA PPHIYSIADNAYNDMLRNRENQSMLI TGESGAGKTVNTKRVIQYFAIVAAL GDTPGKKVAALATKTGGTLEDQIIEA NPAMEAFGNAKTIRNDNSSRFGKFIR IHFGPSGKLASADIDIYLLEKSRVIFQ QPKERSYHIYYQILSGKKPELQDMLL LSLNPYDYHFCSQGVTTVDNLDDGE ELMATDHAMDILGFSNDEKYGSYKI VGAIMHFGNMKFKQKQREEQAEAD GTESADKAAYLMGISSADLIKGLLHP RVKVGNEYVTKGQNVEQVVYAVGA LAKATYDRMFKWLVTRINKTLDTKL ARQFFIGVLDIAGFEIFDFNSFEQLCIN FTNEKLQQFFNHHMFVLEQEEYKKE GIEWVFIDFGLDLQACIDLIEKPLGIL SILEEECMFPKASDMSFKAKLYDNH LGKSPNFQKPRPDKKRKYEAHFELV HYAGVVPYNIIGWLDKNKDPLNETV VAVFQKSQNKLLASLYENYVGSSSE EPHKPGSKEKRKKAASFQTVSQLHK ENLNKLMTNLRSTQPHFVRCIIPNET KTPGAMDAFLVLHQLRCNGVLEGIR ICRKGFPNRILYADFKQRYRILNPAAI PDDKFVDSRKATEKLLSSLELDHSQY KFGHTKVFFKAGLLGMLEEMRDERL AKILTMLQARIRGHLMRIEYQKIISRR EALYTIQWNIRAFNAVKNWSWMKL FFKIKPLLKSAQTEKEMSTLKEEFQK LKEALEKSEAKRKELEEKQVSMIQE KNDLALQLQAEQDNLADAEERCDLL IKSKIQLEAKVKELTERVEDEEEMNA DLTAKKRKLEDECAELKKDIDDLEIT LAKVEKEKHATENKVKNLIEEMAAL DEIIAKLTKEKKALQEAHQQALDDL QAEEDKVNTLTKAKVKLEQQVDDL ESSLEQEKKIRMDLERAKRKLEGDL KLTQESVMDLENDKQQLEEKLKKK DFEMSQLNSRIEDQQVTEAQLQKKIK ELQARIEELEEELEAERAARAKVEKQ RAEVSRELEELSERLEEAGGATSAQL EMNKKREVEFLKLRRDLEEATLQHE STAAALRKKHADSVAELSEQIDNLQ RVKQKLEKEKSEMKMEVDDLSSNIE YLTKNKANAEKLCRTYEDQLSEAKS KVDELQRQLTDVSTQRGRLQTENGE LSRLLEEKESFINQLSRGKTSFTQTIE ELKRQLEEETKSKNALAHALQASRH DCDLLREQYEEEVEAKSELQRNLSK ANAEVAQWRTKYETDAIQRTEELEE AKKKLAIRLQEAEEAVEAAHAKCSS LEKTKHRLQTEIEDLSVDLERANSAC AALDKKQRNFDRILAEWKQKYEETQ AELEASQKESRSLSTELFKLKNAYEE SLDNLETLKRENKNLQEEIADLTDQI SMSGKTIHELEKLKKALENEKSDIQA ALEEAEGALEHEESKTLRIQLELNQI KADVDRKLAEKDEEFENLRRNHQR AMDSMQATLDAEARAKNEAVRLRK KMEGDLNEMEIQLSHANRQAAEFQK LGRQLQAQIKDLQIELDDTQRQNDD LKEQAAALERRNNLLLAEVEELRAA LEQAERSRKLAEQELLEATERVNLL HSQNTGLINQKKKLETDISQLSSEVE DAVQECRNAEEKAKKAITDAAMMA EELKKEQDTSAHLERMKKNMEQTIK DLQMRLDEAEQIALKGGKKQIQKLE ARVRELEGELDMEQKKMAEAQKGI RKYERRIKELSYQTEEDRKNLTRMQ DLIDKLQSKVKSYKRQFEEAEQQAN SNLVKYRKVQHELDDAEERADIAET QVNKLRARTKEVITFKHE SEQ ID P79293 Myosin-7/Sus MVDAEMAAFGEAAPYLRKSEKERL NO: 110 (UniProtKB) scrofa EAQTRPFDLKKDVYVPDDKEEFVKA KILSREGGKVTAETEHGKTVTVKED QVLQQNPPKFDKIEDMAMLTFLHEP AVLYNLKERYASWMIYTYSGLFCVT INPYKWLPVYNAEVVAAYRGKKRSE APPHIFSISDNAYQYMLTDRENQSILI TGESGAGKTVNTKRVIQYFAVIAAIG DRSKKEQTPGKGTLEDQIIQANPALE AFGNAKTVRNDNSSRFGKFIRIHFGA TGKLASADIETYLLEKSRVIFQLKAE RDYHIFYQILSNKKPELLDMLLITNN PYDYAFISQGETTVASIDDAEELMAT DNAFDVLGFTSEEKNSMYKLTGAIM HFGNMKFKLKQREEQAEPDGTEEAD KSAYLMGLNSADLLKGLCHPRVKV GNEYVTKGQNVQQVMYATGALAK AVYEKMFNWMVTRINTTLETKQPR QYFIGVLDIAGFEIFDFNSFEQLCINFT NEKLQQFFNHHMFVLEQEEYKKEGI EWEFIDFGMDLQACIDLIEKPMGIMS ILEEECMFPKATDMTFKAKLYDNHL GKSNNFQKPRNIKGRPEAHFALIHYA GTVDYNIIGWLQKNKDPLNETVVDL YKKSSLKLLSNLFANYAGADTPVEK GKGKAKKGSSFQTVSALHRENLNKL MTNLRSTHPHFVRCIIPNETKSPGVID NPLVMHQLRCNGVLEGIRICRKGFPN RILYGDFRQRYRILNPAAIPEGQFIDS RKGAEKLLGSLDIDHNQYKFGHTKV FFKAGLLGLLEEMRDERLSRIITRIQA QSRGVLSRMEFKKLLERRDSLLIIQW NIRAFMSVKNWPWMKLYFKIKPLLK SAETEKEMATMKEEFGRLKEALEKS EARRKELEEKMVSLLQEKNDLQLQV QAEQDNLADAEERCDQLIKNKIQLE AKVKEMTERLEDEEEMNAELTAKK RKLEDECSELKRDIDDLELTLAKVEK EKHATENKVKNLTEEMAGLDEIIAK LTKEKKALQEAHQQALDDLQAEED KVNTLTKAKVKLEQHVDDLEGSLEQ EKKVRMDLERAKRKLEGDLKLTQES IMDLENDKQQLDERLKKKDFELNAL NARIEDEQALGSQLQKKLKELQARIE ELEEELEAERTARAKVEKLRSDLSRE LEEISERLEEAGGATSVQIEMNKKRE AEFQKMRRDLEEATLQHEATAAALR KKHADSVAELGEQIDNLQRVKQKLE KEKSEFKLELDDVTSNMEQIIKAKAN LEKMCRTLEDQMNEHRSKAEETQRS VNDLTSQRAKLQTENGELSRQLDEK EALISQLTRGKLTYTQQLEDLKRQLE EEVKAKNALAHALQSARHDCDLLRE QYEEETEAKAELQRVLSKANSEVAQ WRTKYETDAIQRTEELEEAKKKLAQ RLQDAEEAVEAVNAKCSSLEKTKHR LQNEIEDLMVDVERSNAAAAALDKK QRNFDKILAEWKQKYEESQSELESSQ KEARSLSTELFKLKNAYEESLEHLET FKRENKNLQEEISDLTEQLGSSGKTI HELEKVRKQLEAEKLELQSALEEAE ASLEHEEGKILRAQLEFNQIKAEMER KLAEKDEEMEQAKRNHLRVVDSLQ TSLDAETRSRNEALRVKKKMEGDLN EMEIQLSHANRMAAEAQKQVKSLQS LLKDTQIQLDDAVRANDDLKENIAIV ERRNNLLQAELEELRAVVEQTERSR KLAEQELIETSERVQLLHSQNTSLINQ KKKMEADLSQLQTEVEEAVQECRN AEEKAKKAITDAAMMAEELKKEQD TSAHLERMKKNMEQTIKDLQHRLDE AEQIALKGGKKQLQKLEARVRELEN ELEAEQKRNAESVKGMRKSERRIKE LTYQTEEDRKNLLRLQDLVDKLQLK VKAYKRQAEEAEEQANTNLSKFRKV QHELDEAEERADIAESQVNKLRAKS RDIGTKGLNEE SEQ ID E1BJ10 Myosin binding MEAATAPEAALRPTLKVKEASPADA NO: 111 (UniProtKB) protein H DGPQASPRRGTGSPLSQLLPPIEEHPK like/Bos taurus IWLPRALRQTYIRKVGDTVNLLIPFQ GKPKPQAIWTRDGCALDTSRVSVRN GERDSILFIREAQRADSGRYQLSVQL GGLEATATIDILVIERPGPPQSIKLVD VWGSSATLEWTPPQDTGNAALLGYT VQKADTKSGLWFTVLERCRRASCTV PNLIVGNSYTFRVFAENQCGLSENAP ITADLAHIQKAATVYRTEGFAQRDFS EAPKFTQPLADCTTVIGYDTQLFCCV RASPKPKIIWLKNKMDIQGNPKYRA LTHLGICSLEIRKPGPFDGGIYTCKAV NPLGEASVDCRVDVKAPN SEQ ID P02540 Desmin/Sus MSQAYSSSQRVSSYRRTFGGAPSFPL NO: 112 (UniProtKB) scrofa GSPLSSPVFPRAGFGTKGSSSSVTSRV YQVSRTSGGAGGLGPLRASRLGATR VPSSSYGAGELLDFSLADAVNQEFLT TRTNEKVELQELNDRFANYIEKVRFL EQQNAALAAEVNRLKGREPTRVAEI YEEELRELRRQVEVLTNQRARVDVE RDNLLDDLQRLKAKLQEEIQLKEEA ENNLAAFRADVDAATLARIDLERRIE SLNEEIAFLKKVHEEEIRELQAQLQE QQVQVEMDMSKPDLTAALRDIRAQ YETIAAKNISEAEEWYKSKVSDLTQA ANKNNDALRQAKQEMMEYRHQIQS YTCEIDALKGTNDSLMRQMRELEDR FASEASGYQDNIARLEEEIRHLKDEM ARHLREYQDLLNVKMALDVEIATYR KLLEGEESRINLPIQTFSALNFRETSPE QRGSEVHTKKTVMIKTIETRDGEVVS EATQQQHEVL SEQ ID F1SFP9 Leiomodin MSEHSRNSDQEEPFDREIDEDEILAN NO: 113 (UniProtKB) 3/Sus scrofa LSPEELKELQSEMDVMAPDPRLPVG MIQKDQTDKPPTGNFDHKSLVDYM YWQKASRRMLEDERVPVTFVPSEEK PQEQRKEIDKGNKNMSQYLKEKLNN EIAAHKRESKSSDNEQETNDDDEDN EDDEDDEEDDAEDEEDEGDESDEKT KGEEEGEVKEPIRNGESNCQQVPNK AFEEQKDRPEAQEKFEKKISKLDPKK LALDTSFLKVSARPSGNQTDLDGSLR RVRQNDPDMKELNLNNIENIPKEML LDFVNAMKKNKHIKTFSLANVGADE SVAFALANMLRENRSITTLNIESNFIT GKGIVAIMRCLQFNETLTELRFHNQR HMLGHHAEMEISRLLKANTTLLKMG YHFELPGPRMVVTNLLTRNQDKQRQ KRQEEQKQQQLKEQKKLIAMLENGL GLPPGMWEMLGGPMPDSQMQEFLQ PPPPKSLHPQTAPFSRRNEVMAKPAQ PPKYRTDPDSFRVVKLKRIQRKSRMP EAREPPEKTNLKDVIKTLKPVPRNRP PPLVEITPRDQLLNDIRHSNIAYLKPV QLPKELA SEQ ID Q5KR49 Tropomyosin MDAIKKKMQMLKLDKENALDRAEQ NO: 114 (UniProtKB) alpha-1 AEADKKAAEDRSKQLEDELVSLQKK chain/Bos taurus LKATEDELDKYSEALKDAQEKLELA EKKATDAEADVASLNRRIQLVEEEL DRAQERLATALQKLEEAEKAADESE RGMKVIESRAQKDEEKMEIQEIQLKE AKHIAEDADRKYEEVARKLVIIESDL ERAEERAELSEGKCAELEEELKTVTN NLKSLEAQAEKYSQKEDKYEEEIKV LSDKLKEAETRAEFAERSVTKLEKSI DDLEDELYAQKLKYKAISEELDHAL NDMTSI SEQ ID F1NK75 Tropomyosin MEAIKKKMQMLKLDKENAIDRAEQ NO: 115 (UniProtKB) 4/Gallus gallus AETDKKAAEDKCKQVEDELVALQK KLKGTEDELDKYSEALKDAQEKLEQ AEKKATDAEGEVAALNRRIQLVEEE LDRAQERLATALQKLEEAEKAADES ERGMKVIENRAMKDEEKMEIQEMQ LKEAKHIAEEADRKYEEVARKLVILE GELERAEERAEVSEVKCSDLEEELKN VTNNLKSLEAQSEKYSEKEDKYEEEI KILSDKLKEAETRAEFAERTVAKLEK SIDDLEDELYAQKLKYKAISEELDHA LNDMTSL SEQ ID H9L074 Tropomyosin/ MEAIKKKMQMLKLDKENALDRAEQ NO: 116 (UniProtKB) Gallus gallus AEAEQKQAEERSKQLEDELAAMQK KLKGTEDELDKYSEALKDAQEKLEL AEKKAADAEAEVASLNRRIQLVEEE LDRAQERLATALQKLEEAEKAADES ERGMKVIENRALKDEEKMELQEIQL KEAKHIAEEADRKYEEVARKLVIIEG DLERTEERAELAESKCSELEEELKNV TNNLKSLEAQAEKYSQKEDKYEEEI KILTDKLKEAETRAEFAERSVAKLEK TIDDLEDELYAQKLKYKAISEELDHA LNDMTSMARRALQESSFGEMGHLP WFPEPQRVYPVSGRVDFFSGMGGLT YGLKRT SEQ ID E1BTG2 Leiomodin- MSTFGYRRELSKYEDIDEDELLASLT NO: 117 (UniProtKB) 2/Gallus gallus EEELKELERELEDIEPDRNLPVGQRQ KSLTEKTPTGTFSREALMAYWERET RKLLEKERLGACEKDSEQEEDNSEDI QEECFTESNSEVSEEAYTEEDDEEEE EEEEEEEDEDDSDDEDEEKQNSAASE RPVNCEDGRSSSHVRHKKCSNAKNS ENLFNGHDGKDTENLSFKSSAIHPCG NPTVIEDALEKVRSNDPETTEVNLNN IENITSQMLIQFSQALRDNTVVKSFSL ANTHADDNVAIAIAGMLKVNQHITS LNIESNFITGKGVLAIMRALQHNKVL TELRFHNQRHIMGSQVEMDIVKLLK ENTTLVKLGYHFDLAGPRMSMTSIL TRNMDKQRQKRMQEQRQQEYGCD GAINPKTKVLQKGTPRSSPYTSPKSSP WSSPKLPRKSAPAKSQPPAPAPPPPPP PPPPPPPPPPPVIPDKKAPTRNIAEVIK QQESSRKALQNGQKKKKGKKGKKH ENSILKEIKDSLKSVSDRKSEEGSRPS TRPSTPQRSLHDNLMEAIRASSIKQL RRVEVPEALR SEQ ID Q6QGC0 PDZ and LIM MPQNVILPGPAPWGFRLSGGIDFNQP NO: 118 (UniProtKB) domain protein LIITRITPGSKAAAANLCPGDVILAID 3/Sus scrofa GYGTESMTHADAQDRIKAAAHQLC LKIDRAETRLWSPQVTEDGKAHPFKI NLESEPQDVNYFEHKHNIRPKPFIIPG RSSGCSTPSGIDGGSGRSTPSSVSTLS TICPGDLKVAAKMAPNIPLEMELPGV KIVHAQFNTPMQLYSDDNIMETLQG QVSTALGETPSMSEPTTASVPPQSDV YRMLHDNRNEPTQPRQSGSFRVLQE LVNDGPDDRPAGTRSVRAPVTKIHG GAGGTQKMPLCDKCGSGIVGAVVK ARDKYRHPECFVCADCNLNLKQKG YFFVEGELYCETHARARMRPPEGYD TVTLYPKA SEQ ID Q3MHM1 Calsequestrin/ MTPSKSSKAPFTFLPSAFLGKKHFAQ NO: 119 (UniProtKB) Bos taurus MKRAHLFVVGVYLLSSCRAEEGLNF PTYDGKDRVVSLTEKNFKQVLKKYD LLCLYYHEPLSSDKVVQKQFQLKEIV LELVAQVLEHKDIGFVMVDAKKEA KLAKKLGFDEEGSLYILKGDRTIEFD GEFAADVLVEFLLDLIEDPVEIINSKL EVQAFERIEDHIKLIGFFKSEESEHYK AFEEAAEHFQPYIKFFATFDKGVAKK LSLKMNEVDFYEPFMDEPIAIPDKPY TEEELVEFVKEHQRPTLRRLRPEDMF ETWEDDLNGIHIVAFAERSDPDGYEF LEILKQVARDNTDNPDLSIVWIDPDD FPLLVAYWEKTFKIDLFKPQIGVVNV TDADSVWMDIPDDDDLPTAEELED WIEDVLSGKINTEDDDNDDEEDEDD DDDDDNSDEEDNDDSDDDDE Smooth muscle tissue SEQ ID P08023 Actin, aortic MCEEEDSTALVCDNGSGLCKAGFAG NO: 120 (UniProtKB) smooth DDAPRAVFPSIVGRPRHQGVMVGM muscle/Gallus GQKDSYVGDEAQSKRGILTLKYPIEH gallus GIITNWDDMEKIWHHSFYNELRVAP EEHPTLLTEAPLNPKANREKMTQIMF ETFNVPAMYVAIQAVLSLYASGRTT GIVLDSGDGVTHNVPIYEGYALPHAI MRLDLAGRDLTDYLMKILSERGYSF VTTAEREIVRDIKEKLCYVALDFENE MATAASSSSLEKSYELPDGQVITIGN ERFRCPETLFQPSFIGMESAGIHETTY NSIMKCDIDIRKDLYANNVLSGGTT MYPGIADRMQKEITALAPSTMKIKII APPERKYSVWIGGSILASLSTFQQMW ISKQEYDEAGPSIVHRKCF SEQ ID P63270 Actin, gamma- MCEEETTALVCDNGSGLCKAGFAGD NO: 121 (UniProtKB) enteric smooth DAPRAVFPSIVGRPRHQGVMVGMG muscle/Gallus QKDSYVGDEAQSKRGILTLKYPIEHG gallus IITNWDDMEKIWHHSFYNELRVAPE EHPTLLTEAPLNPKANREKMTQIMFE TFNVPAMYVAIQAVLSLYASGRTTGI VLDSGDGVTHNVPIYEGYALPHAIM RLDLAGRDLTDYLMKILTERGYSFV TTAEREIVRDIKEKLCYVALDFENEM ATAASSSSLEKSYELPDGQVITIGNER FRCPETLFQPSFIGMESAGIHETTYNS IMKCDIDIRKDLYANNVLSGGTTMY PGIADRMQKEITALAPSTMKIKIIAPP ERKYSVWIGGSILASLSTFQQMWISK PEYDEAGPSIVHRKCF SEQ ID P24032 Myosin MSSKRAKTKTTKKRPQRATSNVFAM NO: 122 (UniProtKB) regulatory light FDQSQIQEFKEAFNMIDQNRDGFIDK chain 2, smooth EDLHDMLASLGKNPTDEYLDAMMN muscle minor EAPGPINFTMFLTMFGEKLNGTDPED isoform/Gallus VIRNAFACFDEEATGFIQEDYLRELL gallus TTMGDRFTDEEVDELYREAPIDKKG NFNYIEFTRILKHGAKDKDD SEQ ID P10587 Myosin- MSQKPLSDDEKFLFVDKNFVNNPLA NO: 123 (UniProtKB) 11/Gallus gallus QADWSAKKLVWVPSEKHGFEAASIK EEKGDEVTVELQENGKKVTLSKDDI QKMNPPKFSKVEDMAELTCLNEASV LHNLRERYFSGLIYTYSGLFCVVINP YKQLPIYSEKIIDMYKGKKRHEMPPH IYAIADTAYRSMLQDREDQSILCTGE SGAGKTENTKKVIQYLAWASSHKG KKDTSITQGPSFSYGELEKQLLQANPI LEAFGNAKTVKNDNSSRFGKFIRINF DVTGYIVGANIETYLLEKSRAIRQAK DERTFHIFYYLIAGASEQMRNDLLLE GFNNYTFLSNGHVPIPAQQDDEMFQ ETLEAMTIMGFTEEEQTSILRVVSSV LQLGNIVFKKERNTDQASMPDNTAA QKVCHLMGINVTDFTRSILTPRIKVG RDVVQKAQTKEQADFAIEALAKAKF ERLFRWILTRVNKALDKTKRQGASF LGILDIAGFEIFEINSFEQLCINYTNEK LQQLFNHTMFILEQEEYQREGIEWNF IDFGLDLQPCIELIERPTNPPGVLALL DEECWFPKATDTSFVEKLIQEQGNH AKFQKSKQLKDKTEFCILHYAGKVT YNASAWLTKNMDPLNDNVTSLLNQ SSDKFVADLWKDVDRIVGLDQMAK MTESSLPSASKTKKGMFRTVGQLYK EQLTKLMTTLRNTNPNFVRCIIPNHE KRAGKLDAHLVLEQLRCNGVLEGIR ICRQGFPNRIVFQEFRQRYEILAANAI PKGFMDGKQACILMIKALELDPNLY RIGQSKIFFRTGVLAHLEEERDLKITD VIIAFQAQCRGYLARKAFAKRQQQL TAMKVIQRNCAAYLKLRNWQWWR LFTKVKPLLQVTRQEEEMQAKDEEL QRTKERQQKAEAELKELEQKHTQLC EEKNLLQEKLQAETELYAEAEEMRV RLAAKKQELEEILHEMEARIEEEEER SQQLQAEKKKMQQQMLDLEEQLEE EEAARQKLQLEKVTADGKIKKMED DILIMEDQNNKLTKERKLLEERVSDL TTNLAEEEEKAKNLTKLKNKHESMI SELEVRLKKEEKSRQELEKIKRKLEG ESSDLHEQIAELQAQIAELKAQLAKK EEELQAALARLEDETSQKNNALKKI RELESHISDLQEDLESEKAARNKAEK QKRDLSEELEALKTELEDTLDTTATQ QELRAKREQEVTVLKRALEEETRTH EAQVQEMRQKHTQAVEELTEQLEQF KRAKANLDKTKQTLEKDNADLANEI RSLSQAKQDVEHKKKKLEVQLQDL QSKYSDGERVRTELNEKVHKLQIEV ENVTSLLNEAESKNIKLTKDVATLGS QLQDTQELLQEETRQKLNVTTKLRQ LEDDKNSLQEQLDEEVEAKQNLERH ISTLTIQLSDSKKKLQEFTATVETMEE GKKKLQREIESLTQQFEEKAASYDKL EKTKNRLQQELDDLVVDLDNQRQL VSNLEKKQKKFDQMLAEEKNISSKY ADERDRAEAEAREKETKALSLARAL EEALEAKEELERTNKMLKAEMEDLV SSKDDVGKNVHELEKSKRTLEQQVE EMKTQLEELEDELQAAEDAKLRLEV NMQAMKSQFERDLQARDEQNEEKR RQLLKQLHEHETELEDERKQRALAA AAKKKLEVDVKDLESQVDSANKAR EEAIKQLRKLQAQMKDYQRDLDDA RAAREEIFATARENEKKAKNLEAELI QLQEDLAAAERARKQADLEKEEMA EELASANSGRTSLQDEKRRLEARIAQ LEEELDEEHSNIETMSDRMRKAVQQ AEQLNNELATERATAQKNENARQQL ERQNKELRSKLQEMEGAVKSKFKST IAALEAKIASLEEQLEQEAREKQAAA KTLRQKDKKLKDALLQVEDERKQA EQYKDQAEKGNLRLKQLKRQLEEAE EESQRINANRRKLQRELDEATESNDA LGREVAALKSKLRRGNEPVSFAPPRR SGGRRVIENATDGGEEEIDGRDGDFN GKASE SEQ ID P02607 Myosin light MCDFSEEQTAEFKEAFQLFDRTGDG NO: 124 (UniProtKB) polypeptide KILYSQCGDVMRALGQNPTNAEVM 6/Gallus gallus KVLGNPKSDEMNLKTLKFEQFLPMM QTIAKNKDQGCFEDYVEGLRVFDKE GNGTVMGAEIRHVLVTLGEKMTEEE VEQLVAGHEDSNGCINYEELVRMVL SG SEQ ID P02542 Desmin/Gallus QSYSSSQRVSSYRRTFGGGTSPVFPR NO: 125 (UniProtKB) gallus ASFGSRGSGSSVTSRVYQVSRTSAVP TLSTFRTTRVTPLRTYGSAYQGAGEL LDFSLADAMNQEFLQTRTNEKVELQ ELNDRFANYIEKVRFLEQQNALMVA EVNRLRGKQPTRVAEMYEEELRELR RQVDALTGQRARVEVERDNLLDNL QKLKQKLQEEIQLKQEAENNLAAFR ADVDAATLARIDLERRIESLQEEIAFL KKVHEEEIRELQAQLQEQHIQVEMDI SKPDLTAALRDIRAQYESIAAKNIAE AEEWYKSKVSDLTQAANKNNDALR QAKQEMLEYRHQIQSYTCEIDALKG TNDSLMRQMREMEERFAGEAGGYQ DTIARLEEEIRHLKDEMARHLREYQD LLNVKMALDVEIATYRKLLEGEENRI SIPMHQTFASALNFRETSPDQRGSEV HTKKTVMIKTIETRDGEVVSEATQQ QHEVL SEQ ID P19352-2 Isoform 2 of MEAIKKKMQMLKLDKENAIDRAEQ NO: 126 (UniProtKB) Tropomyosin AEADKKQAEDRCKQLEEEQQGLQK beta KLKGTEDEVEKYSESVKEAQEKLEQ chain/Gallus AEKKATDAEAEVASLNRRIQLVEEE gallus LDRAQERLATALQKLEEAEKAADES ERGMKVIENRAMKDEEKMELQEMQ LKEAKHIAEEADRKYEEVARKLVVL EGELERSEERAEVAESRVRQLEEELR TMDQSLKSLIASEEEYSTKEDKYEEE IKLLGEKLKEAETRAEFAERSVAKLE KTIDDLEESLASAKEENVGIHQVLDQ TLLELNNL SEQ ID Q2QLE2 Caveolin-2/Sus MGLETEKADVQLFMDDDSYSRHSG NO: 127 (UniProtKB) scrofa VDYADPKKFVDPGTDRDPHRLNSNL KVGFEDVIAEPVSTHSFDKVWICSHA LFEISKYVIYKFLTVFLAIPLAFAAGIL FATLSCLHIWIIIPFVKTCLMVLPSVQ TIWKSVTDVVIAPLCTSAGRSFSSVSL QLSHD SEQ ID Q90623 Protein MKMADAKQKRNEQLKRWIGSETDL NO: 128 (UniProtKB) phosphatase 1 EPPVVKRKKTKVKFDDGAVFLAACS regulatory SGDTEEVLRLLERGADINYANVDGL subunit TALHQACIDDNVDMVKFLVENGANI 12A/Gallus NQPDNEGWIPLHAAASCGYLDIAEY gallus LISQGAHVGAVNSEGDTPLDIAEEEA MEELLQNEVNRQGVDIEAARKEEER IMLRDARQWLNSGHINDVRHAKSGG TALHVAAAKGYTEVLKLLIQARYDV NIKDYDGWTPLHAAAHWGKEEACR ILVENLCDMEAVNKVGQTAFDVADE DILGYLEELQKKQNLLHSEKREKKSP LIESTANLDNNQTQKTFKNKETLIME QEKNASSIESLEHEKADEEEEGKKDE SSCSSEEEEDDDSESEAETDKAKTLA NANTTSTQSASMTAPSVAGGQGTPT SPLKKFPTSTTKVSPKEEERKDESPAS WRLGLRKTGSYGALAEITASKEAQK EKDSAGVIRSASSPRLSSSLDNKEKE KDGKGTRLAYVAPTIPRRLASTSDID EKENRDSSASSIRSGSSYARRKWEED VKKNSLNEGPTSLNTSYQRSGSFGRR QDDLVSSNVPSTASTVTSSAGLQKTL PASANTTTKSTTGSTSAGVQSSTSNR LWAEDSTEKEKDSVPTAVTVPVAPS VVNAAATTTAMTTATSGTVSSTSEV RERRRSYLTPVRDEESESQRKARSRQ ARQSRRSTQGVTLTDLQEAEKTIGRS RSTRTREQENEEKEKEEKEKQDKEK QEEKKESETKDDDYRQRYSRTVEEP YHRYRPTSTSTSTSSTSSLSTSTSSLSS SSQLNRPNSLIGITSAYSRSGTKESER EGGKKEEEKEEDKSQPKSIRERRRPR EKRRSTGVSFWTQDSDENEQEHQSD SEEGTNKKETQSDSLSRYDTGSLSVS SGDRYDSAQGRSGSQSYLEDRKPYC SRLEKEDSTDFKKLYEQILAENEKLK AQLHDTNMELTDLKLQLEKTTQRQE RFADRSLLEMEKRVSGKSQYLLGGK KSSRKKDI SEQ ID P26932-2 Isoform Beta of MSNANFNRGPAYGLSAEVKNKLAQ NO: 129 (UniProtKB) Calponin- KYDPQTERQLRVWIEGATGRRIGDN 1/Gallus gallus FMDGLKDGVILCELINKLQPGSVQK VNDPVQNWHKLENIGNFLRAIKHYG VKPHDIFEANDLFENTNHTQVQSTLI ALASQAKTKGNNVGLGVKYAEKQQ RRFQPEKLREGRNIIGLQMGTNKFAS QQGMTAYGTRRHLYDPKLGTDQPL DQATISLQMGTNKGASQGMTVYGLP RQVYDPKYCDAPGLLGEDGLNHSFY NSQ SEQ ID Q7YRL2 Calponin-1/Ovis MSSAHFNRGPAYGLSAEVKNKLAQ NO: 130 (UniProtKB) aries KYDHQREQELREWIEGVTGRRIGNN FMDGLKDGIILCEFINKLQPGSVKKV NESTQNWHQLENIGNFIKAITKYGVK PHDIFEANDLFENTNHTQVQSTLLAL ASMAKTKGNKVNVGVKYAEKQERK FEPEKLREGRNIIGLQMGTNKFASQQ GMTAYGTRRHLYDPKLGTDQPLDQ ATISLQMGTNKGASQAGMTAPGTKR QIFEPGLGMEHCDTLNVSLQMGSNK GASQRGMTVYGLPRQVYDPKYCLTP EYPELGEPAHNHHPHNYYNSA SEQ ID A0A212D0J2 Calponin/Cervus MSSTQFNKGPSYGLSAEVKNRLQSK NO: 131 (UniProtKB) elaphus YDPQKEAELRSWIEGLTGLSIGPDFQ hippelaphus KGLKDGIILCTLMNKLQPGSIPKINRS MQNWHQLENLSNFIKAMAKTKGLQ SGVDIGLKYSEKQERNFDDATMKAG QCVIGLQVGMTAPGTRRHIYDTKLG TDKCDNSSMSLQMGYTQGANQSGQ VFGLGRQIYDPKYCPQGPVADGAPA AAGDGPGPGEPSECPPYYQEEAGY SEQ ID Q5ZKU6 Calponin/Gallus MSSSQFNKGPSYGLSAEVKNRLAQK NO: 132 (UniProtKB) gallus YDPQKEAELRTWIESVTGRQIGADFQ KGLKDGVILCELMNKLQPNSVRKIN RSALNWHQLENLSNFIKAMVSYGM NPVDLFEANDLFESGNLTQVQVSLL ALAGMAKTKGLQSGVDIGVKYSER QQRNFDEAKMKAGQCVIGLQMGTN KCASQSGMTAYGTRRHLYDPKNQIL PPMDHSTISLQMGTNKCASQVGMTA PGTRRHIYDAKMGLEKCDNSSMSLQ MGSNQGANQSGQVFGLGRQIYDPK YCPQGTPGDAANAAGEPGADPPGYH YYHQEESC SEQ ID E1BSX2 Calponin/Gallus MTHFNKGPSYGLSAEVKNKIALKYD NO: 133 (UniProtKB) gallus PQIEEDLRNWIEEVTGLSIGANFQLG LKDGIILCELINKLQPGSVKKINQSKL NWHQLENIGNFIKAIQVYGMKPHDIF EANDLFENGNMTQVQTTLVALAGL AKTKGFHTTIDIGVKYAEKQARSFD AGKLKAGQSVIGLQMGTNKCASQA GMTAYGTRRHLYDPKMQTDKPFDQ TTISLQMGTNKGASQAGMLAPGTRR DIYDQKHILQPVDNSTISLQMGTNKV ASQKGMSVYGLGRQVYDPKYCAAP TEPVIHNGSQGTGTNGSEISDSDYQA EYPDDYHGEYQDDYQRDYHGQYSD QGIDY SEQ ID P19966 Transgelin/ MANKGPAYGMSRDVQSKIEKKYDD NO: 134 (UniProtKB) Gallus gallus ELEDRLVEWIVAQCGSSVGRPDRGR LGFQVWLKNGIVLSQLVNSLYPDGS KPVKIPDSPPTMVFKQMEQIAQFLKA AEDYGVVKTDMFQTVDLFEAKDMA AVQRTLVALGSLAVTKNDGHYHGD PNWFMKKAQEHKREFSESQLKEGK NIIGLQMGTNKGASQAGMSYGRPRQ IIS SEQ ID A0A1L1RTM1 Caldesmon/ RLSYQRNDDDEEEAARERRRRARQE NO: 135 (UniProtKB) Gallus gallus RLRQKEEGDVSGEVTEKSEVNAQNS VAEEETKRSTDDEAALLERLARREE RRQKRLQEALERQKEFDPTITDGSLS VPSRREVNNVEENETTGKEEKVETR QGRCEIEETETVTKSYQRNNWRQDG EEEGKKEEKDSEEEKPKEVPTEENQV DVAVEKSTDKEEVVETKTLAVNAEN DTNAMLEGEQSITDAADKEKEEAEK EREKLEAEEKERLKAEEEKKAAEEK QKAEEEKKAAEERERAKAEEEKRAA EERERAKAEEERKAAEERERAKAEE ERKAAEERAKAEEERKAAEERAKAE EERKAAEERAKAEKERKAAEERERA KAEEEKRAAEEKARLEAEKLKEKKK MEEKKAQEEKAQANLLRKQEEDKE AKVEAKKESLPEKLQPTSKKDQVKD NKDKEKAPKEEMKSVWDRKRGVPE QKAQNGERELTTPKLKSTENAFGRS NLKGAANAEAGSEKLKEKQQEAAV ELDELKKRREERRKILEEEEQKKKQE EAERKIREEEEKKRMKEEIERRRAEA AEKRQKVPEDGVSEEKKPFKCFSPK GSSLKIEERAEFLNKSAQKSGMKPAH TTAVVSKIDSRLEQYTSAVVGNKAA KPAKPAASDLPVPAEGVRNIKSMWE KGNVFSSPGGTGTPNKETAGLKVGV SSRINEWLTKTPEGNKSPAPKPSDLR PGDVSGKRNLWEKQSVEKPAASSSK VTATGKKSETNGLRQFEKEP SEQ ID E1C2S1 Talin-1/Gallus MVALSLKISIGNVVKTMQFEPSTMV NO: 136 (UniProtKB) gallus YDACRMIRERVPEAQMGQPNDFGLF LSDEDPKKGIWLEAGKALDYYMLR NGDTMEYKKKQRPLKIRMLDGTVK TVMVDDSKTVTDMLMTICARIGITN YDEYSLVREIMEEKKEEVTGTLKKD KTLLRDEKKMEKLKQKLHTDDELN WLDHGRTLREQGIDDNETLLLRRKF FYSDQNVDSRDPVQLNLLYVQARDD ILNGSHPVSFDKACEFAGYQCQIQFG PHNEQKHKPGFLELKDFLPKEYIKQK GERKIFMAHKNCGNMSEIEAKVRYV KLARSLKTYGVSFFLVKEKMKGKNK LVPRLLGITKECVMRVDEKTKEVIQE WSLTNIKRWAASPKSFTLDFGDYQD GYYSVQTTEGEQIAQLIAGYIDIILKK KKSKDHFGLEGDEESTMLEDSVSPK KSTVLQQQFNRVGKAELGSVALPAI MRTGAGGPENFQVGTMPQAQMQIT SGQMHRGHMPPLTSAQQALTGTINS SMQAVNAAQATLDDFETLPPLGQDA ASKAWRKNKMDESKHEIHSQVDAIT AGTASVVNLTAGDPADTDYTAVGC AVTTISSNLTEMSKGVKLLAALMED EGGNGRQLLQAAKNLASAVSDLLKT AQPASAEPRQNLLQAAGLVGQTSGE LLQQIGESDTDPRFQDMLMQLAKAV ASAAAALVLKAKNVAQKTEDSALQ TQVIAAATQCALSTSQLVACTKVVA PTISSPVCQEQLIEAGKLVAKSAEGC VEASKAATNDDQLLKQVGVAATAV TQALNDLLQHIKQHATGGQPIGRYD QATDTILNVTENIFSSMGDAGEMVR QARILAQATSDLVNAIKADAEGETD LENSRKLLSAAKILADATAKMVEAA KGAAAHPDSEEQQQRLREAAEGLR MATNAAAQNAIKKKLVHKLEHAAK QAAASATQTIAAAQHAAASNKNPAA QQQLVQSCKVVADQIPMLVQGVRG SQSQPDSPSAQLALIAASQNFLQPGG KMVAAAKATVPTITDQASAMQLSQ CAKNLAAALAELRTAAQKAQEACG PLEIDSALGLVQSLERDLKEAKAAAR DGKLKPLPGETMEKCAQDLGNSTKA VTSAIAHLLGEVAQGNENYTGIAAR EVAQALRSLSQAARGVAANSSDPQV QNAMLECASDVMDKANNLIEEARK AVAKPGDPDSQQRLVQVAKAVSQA LNRCVNCLPGQRDVDAAIRMVGEAS KRLLSDSFPPSNKTFQEAQSQLNRAA AGLNQSANELVQASRGTPQDLAKSS GKFGQDFNEFLQAGVEMASLSPTKE DQAQVVSNLKSISMSSSKLLLAAKA LSADPTSPNLKSQLAAAARAVTDSIN QLITMCTQQAPGQKECDNALRELET VKELLENPTQTVNDMSYFSCLDSVM ENSKVLGESMAGISQNAKNSKLPEF GESISAASKALCGLTEAAAQAAYLV GVSDPNSQAGQQGLVDPTQFARANQ AIQMACQNLVDPACTQSQVLSAATI VAKHTSALCNTCRLASSRTANPVAK RQFVQSAKEVANSTANLVKTIKALD GAFNEENRERCRAATAPLIEAVDNLT AFASNPEFATVPAQISPEGRRAMEPI VTSAKTMLESSAGLIQTARSLAVNPK DPPQWSVLAGHSRTVSDSIKKLITNM RDKAPGQRECDEAIDVLNRCMREVD QASLAAISQQLAPREGISQEALHNQM ITAVQEINNLIEPVASAARAEASQLG HKVSQMAQYFEPLILAAIGAASKTPN HQQQMNLLDQTKTLAESALQMLYT AKEAGGNPKQAAHTQEALEEAVQM MKEAVEDLTTTLNEAASAAGVVGG MVDSITQAINQLDEGPMGEPEGTFV DYQTTMVKTAKAIAVTVQEMVTKS TTNPDELGILANQLTNDYGQLAQQA KPAALTAENEEIGSHIKRRVQELGHG CAALVTKAGALQCSPSDAYTKKELI ESARKVSEKVSHVLAALQAGNRGTQ ACITAASAVSGIIADLDTTIMFATAGT LNRENSETFADHREGILKTAKALVED TKVLVQNATASQEKLAQAAQSSVST ITRLAEVVKLGAASLGSEDPETQVVL INAVKDVAKALGDLIGATKAAAGKA GDDPAVYQLKNSAKVMVTNVTSLL KTVKAVEDEATKGTRALEATIEHIRQ ELAVFSSPVPPAQVSTPEDFIRMTKGI TMATAKAVAAGNSCRQEDVIATAN LSRRAIADMLRACKEAAYHPEVSAD VRQRALRFGKECADGYLELLEHVLV ILQKPTHELKQQLAGYSKRVASSVTE LIQAAEAMKGTEWVDPEDPTVIAEN ELLGAAAAIEAAAKKLEQLKPRAKP KQADESLDFEEQILEAAKSIAAATSA LVKAASAAQRELVAQGKVGVIPANA VDDGQWSQGLISAARMVAAATNNL CEAANAAVQGHASEEKLISSAKQVA ASTAQLLVACKVKADHDSEAMKRL QAAGNAVKRASDNLVKAAQKAAAF QDHDETVVVKEKMVGGIAQIIAAQE EMLRKERELEEARKKLAMIRQQQYK FLPTELRDEEQN SEQ ID E1BXG1 Profilin/Gallus MNCWNYYTDCILSDKYIDDVAIVGL NO: 137 (UniProtKB) gallus SDNKYVWAAKPGGLLSAVSPREVDL ITGQDRITFLTAGISIAGKKCIVIRDSL LVEGDNVMDIRSRGGDSRSICIGKTP KALIFLMGNRGVHGGVLNLKIHDMI AGMTL SEQ ID E1C9A2 Profilin/Gallus MIQLQALLKESLIRTKHVENAAAIGI NO: 138 (UniProtKB) gallus NEREVCASTSGFYVPPENAINLIYAF YKNLLQVRKEGLYFRQKHYECVRA DEHSIYLKNAEGGLIVVKTNALILIAT YRVGMYPSVCVEAVEKLGKTNTCT CMAACQRFIGWLCSCKELQKCV SEQ ID 093256 Keratin, type 1 MATYSFRQTTSSVAGGPCGRSLRLG NO: 139 (UniProtKB) cytoskeletal GGSFRAPSIHGGSGGRGVSVSSARFV 19/Gallus gallus SSGLGSGLGGGYGGAFSSSFSAGFGG GYGGGLGSGDGLLSGNEKTTMQNL NDRLASYLDKVRALEEANSDLETKI REWYLKQGPGPARDYSPYYKAIEDL RDQILAATIDNSKVVLQIDNARLAAD DFKTKFETEQALRMSVEADINGLRR VLDELTLARTDLELQIENLKEELAYL KKNHEEEMSALGGQVASQVSVEVD SAPGIDLSKILADMRDQYEHMAEKN RKDAEAWFHSKTEELNRELAVNTEQ LQSSKSEVTDLRRTLQGLEIELQSQLS MKGALESTLADTEGRYGAQLAQIQD MIGSIEAQLAELRADMERQNSEYKM LMDIKTRLEQEIATYRQLLEGQESQL FGSLSGSPDKRDKPADGK Skeletal and cardiac muscle tissue SEQ ID B5X7T1 Troponin C, MDDVYKAAVENLTEEQKNEFKAAF NO: 140 (UniProtKB) slow skeletal DIACQGAEDGCISTKELGKVMRMLG and cardiac QNPTPEELQEMIDEVDEDGSGTVDF muscles/Salmo DEFLVMMVRCMKEESKGKSEEELAE salar LFRMFDKNGDGYIDLEELKTMLEST GEAITEDDIEELMKDGDKNNDGKID YDEFLEFMKGVE SEQ ID P09860 Troponin C, MDDIYKAAVEQLTEEQKNEFKAAFD NO: 141 (UniProtKB) slow skeletal IFVLGAEDGCISTKELGKVMRMLGQ and cardiac NPTPEELQEMIDEVDEDGSGTVDFDE muscles/Gallus FLVMMVRCMKDDSKGKTEEELSDL gallus FRMFDKNADGYIDLEELKIMLQATG ETITEDDIEELMKDGDKNNDGRIDYD EFLEFMKGVE SEQ ID P63315 Troponin C, MDDIYKAAVEQLTEEQKNEFKAAFD NO: 142 (UniProtKB) slow skeletal IFVLGAEDGCISTKELGKVMRMLGQ and cardiac NPTPEELQEMIDEVDEDGSGTVDFDE muscles/Bos FLVMMVRCMKDDSKGKSEEELSDLF taurus RMFDKNADGYIDLEELKIMLQATGE TITEDDIEELMKDGDKNNDGRIDYDE FLEFMKGVE SEQ ID P14315-2 Isoform 2 of F- MSDQQLDCALDLMRRLPPQQIEKNL NO: 143 (UniProtKB) actin-capping SDLIDLVPSLCEDLLSSVDQPLKIARD protein subunit KVVGKDYLLCDYNRDGDSYRSPWS beta isoforms 1 NKYDPPLEDGAMPSARLRKLEVEAN and 2/Gallus NAFDQYRDLYFEGGVSSVYLWDLD gallus HGFAGVILIKKAGDGSKKIKGCWDSI HVVEVQEKSSGRTAHYKLTSTVML WLQTNKTGSGTMNLGGSLTRQMEK DETVSDSSPHIANIGRLVEDMENKIR STLNEIYFGKTKDIVNGLRSVQTFAD KSKQEALKNDLVEALKRKQQS SEQ ID E1BMP3 Myosin light MSGAPKESLGPQGLPGLGKACLTTM NO: 144 (UniProtKB) chain kinase DKKLNMLNEKVDKLLHFQEDVTEK 3/Bos taurus LQCVYRGMGHLEQGLHRLEASRGL GPAGADRSPPSDAQAGWPEVLELVR AGRQDAAQQGARLEALFRMVMAV DKAIALVGAVLQNSKVVDFIMQGSV PWRKGSLADNKEQVEEKEAKPKHTL STRGVQAELRGPWEESQKADLPEGT GSDLPTQTEAPPEQRDGISGPTQVRP EVEVQAPRASSKNPGIGLELSVVSER VSEAPTGQEAALSAGRGTSPSRPDPR PSVEGMRLTPAPPAQAKAAHGGGET PPRISIHVQETDTPGELLVTRGGSLRT SPPAETPAAVPPGEQDPPGPRCCPQA PGTESGKPILRGASVRKRSCDEGAKA KEKQGPGSELTMAPSRARRDKGADS GASGPQQDMNPGAGNPDPGKDCTA GGVGSAEAGSRTPPGAEASSLVLDD SPAPPAPFEHRVVSVRETSTSAGYTV CQHEVLGGGRFGQVHRCTEKATGLS LAAKIIKVKSAKDREDVKNEINIMNQ LSHVNLIQLYDAFESKNSFTLVMEYV DGGELFDRITEEKYHLTELDVVLFTK QICEGVHYLHQHYVLHLDLKPENILC VNQTGHQIKIIDFGLARRYKPREKLK VNFGTPEFLAPEVVNYEFVSFPTDM WSVGVITYMLLSGLSPFLGETDAET MNFIVNCNWDFDADTFEGLSEEAKD FVSRLLVKEKSCRMSATQCLKHEWL NNLPAKASKSKVHLKSQLLLQKYM AQRKWKKHFYVVTAANRLRKFPTC P SEQ ID B5X8Q3 Troponin MNDIYKAAVEQLTDEQKNEFKAAFD NO: 145 (UniProtKB) C/Salmo salar IFVQDAEDGCISTKELGKVMRMLGQ NPTPEELQEMIDEVDEDGSGTVDFDE FLVMMVRCMKDDSKGKTEEELADL FRMFDKNADGYIDLEELKVMLEATG EAITEDDIEELMKDGDKNNDGKIDY DEFLEFMKGVE SEQ ID A4GR69 Telethonin/Sus MATSELSCQVSEENCERREAFWAEW NO: 146 (UniProtKB) scrofa KDLTLSTRPEEGCSLHEEDAERRETY HQQGQCQALVQRSPWLVMRMGILG RGLQEYQLPYQRVLPLPIFTPAKVGA AKEEREETPIQLRELLALETALGGQC LDRQDVAEITKQLPPVVPVSKPGALR RSLSRSMSQEAQRG SEQ ID P04268 Tropomyosin MDAIKKKMQMLKLDKENALDRAEQ NO: 147 (UniProtKB) alpha-1 AEADKKAAEERSKQLEDELVALQKK chain/Gallus LKGTEDELDKYSESLKDAQEKLELA gallus DKKATDAESEVASLNRRIQLVEEELD RAQERLATALQKLEEAEKAADESER GMKVIENRAQKDEEKMEIQEIQLKE AKHIAEEADRKYEEVARKLVIIEGDL ERAEERAELSESKCAELEEELKTVTN NLKSLEAQAEKYSQKEDKYEEEIKV LTDKLKEAETRAEFAERSVTKLEKSI DDLEDELYAQKLKYKAISEELDHAL NDMTSI SEQ ID Q05706 Beta- MEAIKKKMQMLKLDKENAIDRAEQ NO: 148 (UniProtKB) tropomyosin/ AEADKKQAEDRCKQLEEEQQGLQK Gallus gallus KLKGTEDEVEKYSESVKEAQEKLEQ AEKKATDAEAEVASLNRRIQLVEEE LDRAQERLATALQKLEEAEKAADES ERGMKVIENRAMKDEEKMELQEMQ LKEAKHIAEEADRKYEEVARKLVVL EGELERSEERAEVAESKCGDLEEELK IVTNNLKSLEAQADKYSTKEDKYEE EIKLLGEKLKEAETRAEFAERSVAKL EKTIDDLEERSRQEAEKNRVLTNELR VILTELNN SEQ ID Q2KI43 Caveolin-3/Bos MMAEEHTDLEAQIVKDIHFKEIDLV NO: 149 (UniProtKB) taurus NRDPKNINEDIVKVDFEDVIAEPVGT YSFDGVWKVSYTTFTVSKYWCYRL LSTLLGVPLALLWGFLFACISFCHIW AVVPCIKSYLIEIQCISHIYSLCIRTFC NPLFAALGQVCSNIKVMLRKEV SEQ ID Q02173 M-protein, MSSVAVPFYQRRHKHFDQSYRNIQT NO: 150 (UniProtKB) striated RYVLEEYAARKAASRQAAHYESTGL muscle/Gallus GKTTCRLCARRARSLAHEAMQESRK gallus RTHEQKSHASDEKRIKFASELSSLER EIHMARHHAREQLDRLAIQRMVEEN MALERHVVEEKISRAPEILVRLRSHT VWEKMSVRLCFTVQGFPSPVVQWY KNEELITPASDPAKYSVENKYGVHV LHINRADFDDSATYSAVATNIHGQAS TNCAVVVRRFRESEEPHPAGIMPFHL PLSYDVCFTHFDVQFLEKFGVTFATE GETLTLKCSVLVTPELKRLRPRAEW YRDDVLIKDSKWTKLYFGEGQAALS FTHLNKDDEGLYTLRMVTKGGVNE CSAFLFVRDADALIAGAPGAPMDVK CHDANRDYVIVTWKPPNTTSQNPVI GYFVDKCEVGLENWVQCNDAPVKI CKYPVTGLYEGRSYIFRVRAVNSAGI SRPSRVSEPVAALDPVDLERTQTVHV DEGRKIVISKDDLEGDIQIPGPPTNVH ASEISKTYVVLSWDPPVPRGREPLTY FIEKSMVGSGSWQRVNAQVAVKSPR YAVFDLAEGKPYVFRVLSANKHGIS DPSEITEPIQPQDIVVVPSAPGRVVAT RNTKTSVVVQWDKPKHEENLYGYYI DYSVVGSNQWEPANHKPINYNRFVV HGLETGEQYIFRVKAVNAVGFSENS QESEAIKVQAALTCPSYPHGITLLNC DGHSMTLGWKAPKYSGGSPILGYYI DKREANHKNWHEVNSSVISRTIYTV EDLTEDAFYEFKIAAANVVGIGHPSD PSEHFKCKAWTMPEPGPAYDLTVCE VRNTSLVLLWKAPVYEGKSPITGYL VDYKEVDTEDWITANEKPTSHRYFK VTDLHQGHTYVFKVRAVNDAGVGK SSEISEPVFVEASPGTKEIFSGVDEEG NIYLGFECKEATDASHFLWGKSYEEI EDSDKFKIETKGDHSKLYFKHPDKSD LGTYCISVSDTDGVSSSFVLDEEELE RLMTLSNEIKNPTIPLKSELAYEVLD KGEVRFWIQAESLSPNSTYRFVINDK EVENGDRHKISCDHSNGIIEMVMDK FTIDNEGTYTVQIQDGKAKNQSSLVL IGDAFKAILAESELQRKEFLRKQGPH FSEFLYWEVTEECEVLLACKIANTKK ETVFKWYRNGSGIDVDEAPDLQKGE CHLTVPKLSRKDEGVYKATLSDDRG HDVSTLELSGKVYNDIILALSRVSGK TASPLKILCTEEGIRLQCFLKYYNEE MKVTWSHRESKISSGEKMKIGGGED VAWLQITEPTEKDKGNYTFEIFSDKE SFKRTLDLSGQAFDDALTEFQRLKA AAFAEKNRGKVIGGLPDVVTIMDGK TLNLTCTVFGNPDPEVVWFKNDKAL ELNEHYLVSLEQGKYASLTIKGVTSE DSGKYSIYVKNKYGGETVDVTVSVY RHGEKIPEVNQGQLAKPRLIPPSSST SEQ ID E1BE25 Filamin C/Bos MMNNSGYSEAPGFGLGDEVDDMPS NO: 151 (UniProtKB) taurus TEKDLAEDAPWKKIQQNTFTRWCNE HLKCVGKRLTDLQRDLSDGLRLIAL LEVLSQKRMYRKFHPRPNFRQMKLE NVSVALEFLEREHIKLVSIDSKAIVDG NLKLILGLIWTLILHYSISMPMWEDE DDEDARKQTPKQRLLGWIQNKVPQL PITNFNRDWQDGKALGALVDNCAPG LCPDWEAWDPNQPVENAREAMQQA DDWLGVPQVIAPEEIVDPNVDEHSV MTYLSQFPKAKLKPGAPVRSKQLNP KKAIAYGPGIEPQGNTVLQPAHFTVQ TVDAGIGEVLVYIEDPEGHTEEAKVV PNNDKNRTYAVSYVPKVAGLHKVT VLFAGQNIERSPFEVNVGMALGDAN KVSARGPGLEPVGNVANKPTYFDIY TAGAGTGDVAVVIVDPQGRRDTVEV ALEDKGDSTFRCTYRPVMEGPHTVH VAFAGAPITRSPFPVHVAEACNPNAC RASGRGLQPKGVRVKEVADFKVFTK GAGSGELKVTVKGPRGTEEPVKVRE AGDGVFECEYYPVVPGKYVVTITWG GYAIPRSPFEVQVSPEAGIQKVRAWG PGLETGQVGKSADFVVEAIGTEVGT LGFSIEGPSQAKIECDDKGDGSCDVR YWPTEPGEYAVHVICDDEDIRDSPFI AHIQPAPPDCFPDKVKAFGPGLEPTG CIVDKPAEFTIDARAAGKGDLKLYA QDADGCPIDIKVIPNGDGTFRCSYVP TKPIKHTIIVSWGGVNVPKSPFRVNV GEGSHPERVKVYGPGVEKTGLKANE PTYFTVDCSEAGQGDVSIGIKCAPGV VGPAEADIDFDIIKNDNDTFTVKYTP PGAGRYTIMVLFANQEIPASPFHIKV DPSHDASKVKAEGPGLNRTGVEVGK PTHFTVLTKGAGKAKLDVHFAGAA KGEAVRDFEIIDNHDYSYTVKYTAV QQGNMAVTVTYGGDPVPKSPFVVN VAPPLDLSKVKVQGLNSKVAVGQEQ AFSVNTRGAGGQGQLDVRMTSPSRR PIPCKLEPGGGAETQAVRYMPPEEGP YKVDITYDGHPVPGSPFAVEGVLPPD PSKVCAYGPGLKGGLVGTPAPFSIDT KGAGTGGLGLTVEGPCEAKIECQDN GDGSCAVSYLPTEPGEYTINILFAEA HIPGSPFKATIRPVFDPSKVRASGPGL ERGKAGEAATFTVDCSEAGEAELTIE ILSDAGVKAEVLIHNNADGTYHITYS PAFPGTYTITIKYGGHPVPKFPTRVH VQPAVDTSGVKVSGPGVEPHGVLRE VTTEFTVDARSLTATGGNHVTARVL NPSGAKTDTYVTDNGDGTYRVQYT AYEEGAHLVEVLYDDVAVPKSPFRV GVTEGCDPTRVRAFGPGLEGGLVNK ANRFTVETRGAGTGGLGLAIEGPSEA KMSCKDNKDGSCTVEYIPFTPGDYD VNITFGGRPIPGSPFRVPVKDVVDPG KVKCSGPGLGAGVRARVPQTFTVDC SQAGRAPLQVAVLGPTGVAEPVEVR DNGDGTHTVHYTPATDGPYTVAVK YADQEVPRSLSSPFKIKVLPAHDASK VRASGPGLNASGIPASLPVEFTIDAR DAGEGLLTVQILDPEGKPKKANIRDN GDGTYTVSYLPDMSGRYTITIKYGG DEIPYSPFRIHALPTGDASKCLVTVSI GGHGLGACLGPRIQIGEETVITVDAK AAGKGKVTCTVSTPDGAELDVDVV ENHDGTFDIYYTAPEPGKYVITIRFG GEHIPNSPFHVLACEAMPRVEEPPDV PQLHRPSAYPTHWATEEPVVPAEPM ESMLRPFNLVIPFTVQKGELTGEVRM PSGKTARPNITDNKDGTITVRYAPTE KGLHQMGIKYDGNHIPGSPLQFYVD AINSRHVSAYGPGLSHGMVNKPATF TIVTKDAGEGGLSLAVEGPSKAEITC KDNKDGTCTVSYLPTAPGDYSIIVRF DDKHIPGSPFTAKITGDDSMRTSQLN VGTSTDVSLKITESDLSQLTASIRAPS GNEEPCLLKRLPNRHIGISFTPKEVGE HVVSVRKSGKHVTNSPFKILVGPSEI GDASKVRVWGKGLSEGHTFQVAEFI VDTRNAGYGGLGLSIEGPSKVDINCE DMEDGTCKVTYCPTEPGTYIINIKFA DKHVPGSPFTVKVTGEGRMKESITR RRQAPSIATIGSTCDLNLKIPGNWFQ MVSAQERLTRTFTRSSHTYTRTERTE ISKTRGGETKREVRVEESTQVGGDPF PAVFGDFLGRERLGSFGSITRQQEGE ASSQDMTAQVTSPSGKTEAAEIVEGE DSAYSVRFVPQEMGPHTVTVKYRGQ HVPGSPFQFTVGPLGEGGAHKVRAG GTGLERGVAGVPAEFSIWTREAGAG GLSIAVEGPSKAEIAFEDRKDGSCGV SYVVQEPGDYEVSIKFNDEHIPDSPF VVPVASLSDDARRLTVTSLQETGLK VNQPASFAVQLNGARGVIDARVHTP SGAVEECYVSELDSDKHTIRFIPHEN GVHSIDVKFNGAHIPGSPFKIRVGEQ SQAGDPGLVSAYGPGLEGGTTGVSS EFIVNTLNAGSGALSVTIDGPSKVQL DCRECPEGHVVTYTPMAPGNYLIAIK YGGPQHIVGSPFKAKVTGPRLSGGHS LHETSTVLVETVTKSSSSRGSSYSSIP KFSSDASKVVTRGPGLSQAFVGQKN SFTVDCSKAGTNMMMVGVHGPKTP CEEVYVKHMGNRVYNVTYTVKEKG DYILIVKWGDESVPGSPFKVNVP SEQ ID Q5ZLY3 Tropomodulin MTLPFRKDLDKYKDLDEDDILGKLS NO: 152 (UniProtKB) 3/Gallus gallus EEELKQLETVLDDLDPENALLPAGFR QKDQTAKKASGPFDRERLLAYLEKQ ALEHKDREDYVPFTKEKKGKVFIPK QKPAQSYAEEKIALDPELEEALTSAT DTELCDLAAILGMSNLITNNQFCDIV GSSNGVGKDSFSNIVKGEKMLPVFD EPPNPTNVEETLQRIKDNDSRLVEVN LNNIKNIPIPTLKEFAKALETNTHVKN FSLAATRSNDPVAVALADMLRVNTK LKSLNIESNFITGVGILALVDALKDNE TLTEIKIDNQRQQLGTLAEVEIAKML EENTKILKFGYHFTQQGPRARAAAAI TKNNDLVRKRRVEGDGQ SEQ ID AAC14459.1 Tropomodulin/ MSYRKELEKYRDLDEDKILGALTEE NO: 153 (GenBank) Gallus gallus ELRKLENELEELDPDNALLPAGLRQR DQTQKPPTGPFKREELMAHLEQQAK DIKDREDLVPFTGEKRGKAWIPKQK PMDPVLESVTLEPELEEALANASDAE LCDIAAILGMHTLMSNQQYYEALGS STIVNKEGLNSVIKPTKYKPVPDEEP NSTDVEETLKRIQNNDPDLEEVNLN NIMNIPVPTLKALAEALKTNTYVKKF SIVGTRSNDPVAFALAEMLKVNNTL KSLNVESNFISGSGILALVEALQSNTS LIELRIDNQSQPLGNNVEMEIANMLE KNTTLLKFGYHFTQQGPRLRASNAM MNNNDLVRKRRLAELNGPIFPKCRT GV SEQ ID A0A287BJ41 Tropomodulin MSYRRELEKYRDLDEDEILGALTEEE NO: 154 (UniProtKB) 1/Sus scrofa LRTLENELDELDPDNALLPAGLRQK DQTTKAPTGPFKREELLDHLEKQAK EFKDREDLVPYTGEKRGKVWVPKQ KPMDPVLETVTLEPELEEALANASD AELCDIAAILGMHTLMSNQQYYQAL GSSSIVNKEGLNSVIKPTQYKPVPDE EPNATDVEETLERIKNNDPKLEEVNL NNIRNIPIPTLKAYAEALKENSYVKK FSIVGTRSNDPVAFALAEMLKVNKV LKTLNVESNFISGAGILRLVEALPYN TSLVELKIDNQSQPLGNKVEMEIVSM LEKNATLLKFGYHFTQQGPRLRASN AMMNNNDLVRKRRLADLTGPIIPKC RSGI SEQ ID F1NXA5 Coronin/Gallus MSRRVVRQSKFRHVFGQPVKADQM NO: 155 (UniProtKB) gallus YEDIRVSKVTWDSSFCAVNPKFVAII VEAGGGGAFMVLPLAKTGRVDKNH PLVTGHTAPVLDIDWCPHNDNVIAS ASEDTTVMVWQIPDYVPVRSITEPVV TLEGHSKRVGIICWHPTARNVLLSAG CDNLVILWNVGTGEMLLALEDMHT DLIYNVGWNRNGSLLVTTCKDKKV RVIDPRKQTVVAEITKPHDGARPIRAI FMADGKIFTTGFSKMSERQLGLWDL KNFEEPIALQEMDTSNGVLLPFYDPD TNIVYLCGKGDSSIRYFEITDEAPYV HYLNTYSSKEPQRGMGFMPKRGLD VSKCEIARFFKLHERKCEPIVMTVPR KSDLFQDDLYPDTPGPEPALEADEW LSGKDAEPILISLRDGYVPVKNRELK VVKKNILDSKPPPGPRRSHSTSNTDIS TPALDEVLEEIRVLKETVQAQEKRIS ALEHKLCQFTNGTD SEQ ID XP_015136760.1 Nebulette MYFSFMLAGLHRRKEFSLLFPPSIKM NO: 156 (NCBI) isoform RVSVTQEFTEDENENGEEERVFLKPV X1/Gallus gallus IEDRNMELARKCSEIISDVHYKEEFE KSKGKCIFVPDTPQLKHVKSVGAFIS EVKYKGAAKKDLSNSLYQQMPATID SAFAKELTQLQSKVLYKQKHDAEKG TSDYAHMKEPPDIKHAMEVNKYQS DVSYKRDVQDTHRYTEVLNRPDIKM ATEITKIISDAEYKKGRGEMNKEPAV LGRPDFEHAKGVSKLLSQVKYKEQF KKEMKSHQYNPLDSASFKQAQIAST LASNVNYKKDYKESLHDPASDLPNL LYLNHALNISKMHSDVKYRENYEKS KGKSMLEFVDTPLYQVSKDVQKMQ SEKIYRKDFEESLKGRPSLDLDKTPEF LHIKQVTNLLKEKEYRKDLEEWMK GKGMTVFEDTPDLIRVKNAAQILNE KQYKKDLETEIKGKGMQVGPDTPEI RRAKKASEIASTKEYKKDLENEIKGK GMEVGMDTPDIQRAKKASEIVSQKE YRKDLETEIIGKGMQVGPFTPEIQRV KRASEIASQKMYKDEAEKMLCNYSA VPDTPEMERIKSTQKNISSVFYKKVV GAGTAVKETPEIERVKKNQQNISSIK YKEETQHATPISDPPELRRIKENQKNI SNVHYKEQLCRATPVSVTPEIERVKR NQENVSMVHYREQPGKATAVSITPEI ERVKKNQDNISSVKYSSDQRQMKGR RSVILDTPELRHVKETQNNISMVKYH EDFEKTKGRGFTPVVDDPITERVRKN TQVVSDAAYKGVHPHIVEMDRRPGII VDLKVWRTDPGSIFDIDPLEDNIQSR SLHMLSERASRYSKQYLHSTSLGDY KSDGSDTNPTFSYCSEITRPSDEGAPV LPGAYQQSQSQGYGYMHQTSMSSM RSVHSQPHPAGLRTYRAMYDYSAQ DEDEVSFRDGDYIINVQPIDDGWMY GTVQRTGKTGMLPANYIEFVN SEQ ID F1MMX2 Nebulin-related- MNVQACSRCGYGVYPAEKINCLDQI NO: 157 (UniProtKB) anchoring WHKACFHCEVCKMMLSVNNFVSYQ protein/Bos KKPYCHAHNPKNNTFTSVYYTPLNL taurus NVRKPPEAICGIGGQEDGERFKSVFH WDMKSKDEAAAPNRQPQVDERAY WSGYREGDAWCPGALPDPEIVRMV EARKSLGEEYPEDYEQQRGKGSFPA MITPAYQRAKIANQLASQVEYKRGH DERISRFSTVADTPELLRAKAGGQLQ SDVRYTEAYEQQRGKGSFPAMITPA YQIAKRANELASDVRYHQQYQREM KGMAGPAAGAEGPLPKEYMDQYGQ GYSEEYGEHRGKGSFPAMITPAYQN AKKANELASDIKYRQDFNKMKGAA HYHSLPAQDNLVLKRAQSVNKLVSE VEYKKDLESSKGHSINYCETPQFRNV CKISKFTSDNKYKENYQNRMRGRYE GVGMDKRMLHALKVGSLASNIAYK ADYKHDVVDYNYPATLTPSYQTTV KLAPLKDVNYRQSIDKLKYSSVTNTP QIVQAKINAQQLSHVNYRADYEKNK LNYTLPQDVPQLVKARTNAELFSEV KYREGWEKTKGKGFEMKLDAMSLL AAKASGELASNIKYKEEYEKAKGKV LGTTDSRLLHSLQVAKMSSEVEYKK GFEKSKTHFHLPMDMVNIRHAKKAQ ALASDLDYRKRLHEYTVLPEDMKTL WAKKAYGLQSELQYKADLAWMKG VRWLTEGSLNLEQAKKAGQLVSEK NYRQRVDELKFTSVADSSQMEHAK KSQELQSGVAYKAEHEQSVHQYSIS KDEPLFLQARANAANLSEKLYKSSW ENQKAKAFDLRLDSLAFLAAKAKRD LASEVKYKEDYEKSRGKLIGAQGAQ GDSQMSHSLQMSKLQSELEYKKGFE DTKSQCHVPLDMIHLVHARKAQHLA TDIGYKTASHHFTALPTDMKVEWAK KAYGLQSDNQYRADVKWMKGTGW VATGSLNVEQAKKAGELISEKKYRQ HPDALKFTSIKDTPEMVQARISYTQA VDRLYREQGENLKHHYTQTTDLPEV LLAKLNAMNISETRYKESWSKLRDG GYKLRLDAIPFQAAKASGEIISDYKY KEAFEKMKGQMLGSRSLEDDISLAH SVYASSLQSQVNYKKDFEHSKAQFH LPLDMVTLVHAKKAQTLASDQDYR HPLPQYTSLAEDLRLSCAKRAHKLQ SENLYRSDLNFMRGVACVIPGTLEIE GRKRASELISESKYRQHPQSLKYTAV TDTPSLTHAKLSNQITNERLYKAAGE DARHQYTMTLGLPEFVRAKTNAAN LSDAKYKESWRNLHAQGYKLTIDAL PFQAARVSGDIASDFLYRHDFVKER GRLIGAQSVSDDPRLQHCQRVGQLQ SELQYRRQAAGSRAQCHLPMDMVP LVHARKAQALASDLDYRTQCHAFT ALPEDLRMAWAKKAHALQSELRYK SDLMGMKGTGWLALSSPQIESAKKA GELISETKYREKPDTIKFTTVVDSPDL VHAKNSYMHCNERLYRSGDAESRH RYTLVPDHPDFTRARLNALNLSDKV YRHSWEQTRAGGYDFRLDAIPFQTA RASREIASDFRYKEAFLRDRGLQIGY RSINDDPRTKHFLSVGRLQSDNEYKK DFAKSRSQFHSRPDQPGFLQAKRSQ QLASDVHYRQPLPQPTCDPEQLGLK HAQKAHRLQSDVKYKSDLNLTRGIG WTPPGSYKVEMARRAAELANARGL GLQGAYGGPEAVEPRDDQSGFVNPD ATEILHVKRRKAPLF SEQ ID XP_003641574.1 nebulin-related- MNVQPCARCGYGVYPAEKINCIDQT NO: 158 (NCBI) anchoring WHKACFHCEVCKMMLTVNNFVSHE protein isoform KKPYCQVHNPKNNAFTSIFETPINLN X1/Gallus gallus AKKLSEVVSEVKYREESEQFKSAFQ WDVRSRDIEAAYKAQHLSSQNAYY AAYEGGTSWYSGNMPDPQMVTVTQ AQKNLNDLQYTEEYELRQGKGSFPA MITPGYQVAKRATQLSSNVEYRRGH EERVSKFTSVVDTPDILHAKAGGQL ASDLKYTEDFEEQRGKGSFPAMITPA YQIAKRANELASDVKYHQTYEKEIK GKASHTAGTDVTFTRENVDQYGQD YMNEYEERRGKGSFPAMITPAYQNA KKANELASDIKYKKDLSKMKGAAH FHSLTAEDNLVLKQAQSANKLVSEV EYKKDLGNNRGYSVNYCDTPQFKN VSKISKYTSDIKYKETYQNQMKGHY MGIGMDKRMLHAMKVGNLASNIAY KSDYKHDGVDYNYPATLTPSYQTTR KLVPLKDVNYRQSIDKMKYSSVAST PEIAQAKINAQQLSDLNYRAQYEKT KTNYTLPQDIPQLVKAKANAELYSE VKYKEGWEKSKGQGFEMKLDSLPL LAAKASRDLASDIKYKEEYEKTKGK AIETKDSRLLHSLQVAKMSSEIAYKK DFEESKTHFHLPMDMVNLRHAKKA QALASDLDYRKRLHEYTVVPEDLKT KWAKKAYGLQSDLQYREDLMWMK GVGCITEGSLNIQQAKKAGDLVSEK KYRQKVDALKFTSVADSSHIKHAKK SQELQSDVAYRSGKEQFLHQYTITK DDPVFLLAKANAANISEKLYRSSWE KQKEKGFVLRLDALSFLTAKAKRDL ASDIKYKEGYEKMKGKLIGVKAVEE DSKMAHSLQMSKLQSDLEYKKAFE DTKNQFQVSMDMANLVHAKKAQN LASDKGYRTALHHYTVLPTDRKVA WAKWAYGLQSDNRYRADLNWMKG AGWIATGSLNVEQAKKAGELISEKK YRQHPYALKFTSIKDTPEMIQARISY NQAVDRLYKEHGESIKHQYTLTADL PEILRAKLNTMNISEIRYKESWQKMK DGGYQLRLDAIPFQAAKASGEIISDH KYKEAFEKMKGQMIGSCGLDGDIRI AHSVHASSLQSDVKYKQGFEDTKTH FHLPLDMINLVHARKAQSLVSEQEY RQLLHQYTSLTDDLRLQCAKNAYKL QSENLYRSDMNFMRGVGCITPGALEI EGKKKASELISESKYRQQPHSFKYTS VTDSPGLLHAKFSNMIANERLYKAA GEDIQHHYTPTLGLPEFTQARINAAN LSDVKYRESWHNLCAQGYKLTMEA LPFQTARASREIASDYQYKHNFVME RGKHIGARSVLDDTRLLHCLHAAKL QSEQEYKKGSQGVWSQYYLPMDMV NLVHARKAQALASDQEYRKRLHEFT ALPEDLKMKWAKRAHMLQSEHRYK SDLNFMKGVGWMALRSPQIESVKK AGELISETKYRQKPESLKFTAVVDSP DLIHAKNSYLQCNDRLYKAGDSEAR HRYTLPPDHPDFIRARQNALNISDKV YKTSWEQTRASGYDFRIDAIPFQTAK ASREIASDYKYREAFLRDRGQRIGFF SANDDAHTRHVLRVGKLQSDNLYRS GYAQNRGHFQSHLNQPGFLHAKRSQ QLASNVNYKQPLHQYTCDPEQLNVK HAKQAYKLQSDVKYKSDLNWLRGI GWTPPGSYKVERARRAAELAYLRE MGLQAASAQYGPEADQVGPEGSQQ VTVNPDASEILQVKRRKMQLYK SEQ ID P08728 Keratin, type 1 MTSYSYRQSSSTSSFGGMGGGSMRF NO: 159 (UniProtKB) cytoskeletal GAGGAFRAPSIHGGSGGRGVSVSSA 19/Bos taurus RFVSSSSGGYGGGYGGALATSDGLL AGNEKLTMQNLNDRLASYLEKVRA LEEANGDLEVKIRDWYQKQGPGPAR DYSHYFKTIEDLRDQILGATIENSKIV LQIDNARLAADDFRTKFETEQALRM SVEADINGLRRVLDELTLARTDLEM QIEGLKEELAYLKKNHEEEMSVLKG QVGGQVSVEVDSAPGIDLAKILSDM RSQYEVIAEKNRKDAEAWFISQTEEL NREVAGHTEQLQISKTEVTDLRRTLQ GLEIELQSQLSMKAALEGTLAETEAR FGAQLAQIQALISGIEAQLSDVRADT ERQNQEYQHLMDIKTRLEQEIATYR NLLEGQDAYFNDLSLAKAL SEQ ID E1BF23 Myomesin MSLVAVPFYQKRHKHFDQSYRNIQT NO: 160 (UniProtKB) 2/Bos taurus RYLLDEYSAKKKRASAQSSLQRSVT RKSSSQRESSRAALGRTTCRLCAKR MSSALEEEAQERKRRYQSMVAAYG EAKRERYLSELAQLEEDVHVARTHA RDQLDRLALQHAVDDRLAWERHSF EERISRAPEILVRLRSHTVWERMSVK LCFTVQGFPTPVVQWYKDGSLICQA GEPGKYRIDSKYGVHTLEINRADFDD TATYSAVATNVHGQVSTNAAVVVR RFRGDEEPFHSVGLPIGLPLSSVIPYT HFDVQFIEKFGVTFRREGETLTLKCT LLVTPDLKRVQPRAEWYRDDVLLKE SKWTKMFFGEGQASLSFSHLNKDDE GLYTLRIVSRGGITDHSAFLFVRDAD PLVTGAPGAPMDVHCHDVNRDYVI VTWKPPNATKESPVIGYFIDRCEVGT DNWIQCNDAPVKICKYPVTGLFEGR SYIFRVRAVNSAGISRPSRVSEAVAA LDPVDLRRLQAVHLEGEKDIVVYQE ELEGEVQIPGPPTNVHASETSRTYVV LSWDPPEPRGKEPLMYFIEKSMVGS GSWQRVNAQTAVRSPRYAVFDLAE GKPYVFRVLSANKHGLSDPSEITAPI QAQDTIVVPSAPGRVLASRNTRTSVV VQWDRPKHEEDLLGYYVDCSVAGS NVWEPCNHKPIGYNRFVVHGLTTGE QYIFRVKAVNAVGTSENSQESDVIKV QAALTVPSHPYGITLLNCDGHSMILG WKVPKFSGGSPILGYYVDKREAHHK NWHEVNSSPLKERILTVEGLTEGSLY EFKIAAANMAGIGQPSDPSELFKCEA WTMPEPGPAYDLTFCEVRDTSLVLL WKPPVYSGSSPVSGYFVDYKEEDSG EWLTVHETATPHRYLKVCDLHQGK TYVFRVRAVNASGVGRPSDTSEPVL VEARPGTKEVSAGVDEEGNVYLSFD CPEVTDASHFTWCKSYEDIADDDRF KVETAGDHSKLYFKNLDKEDLGTYS VSVSDTDGVSSSFVLDEEELERLKAL SNEIKNPTIPLKSELAYEIFDKGQVRF WLQAEHLSPDSSYRFIINDREVADSE THRIKCDKSTGIIEMVMDRFTIDNEG TYTVQIQDGKAKSQSSLVLIGDAFKA VLKEAEFQRKEFLRKQGPHFAEYLH WDVTEECEVRLVCKVANTKKETVF KWLKDDVLYETEKMPDLEKGVCEL LIPKLSKKDHGEYKATLKDDRGQDV STLEIAGKVYEDMILAMSRVCGASA SPLKILCTPEGIRLQCFMKYFTEEMK VNWYHKDAKITSSEHMRIGGNEQM AWLQICEPTEKDKGKYTFEILDGKK NHQRSLDLSGQAFDEAFAEFQQLKA AAFAEKNRGKVIGGLPDVVTIMEGK TLNLTCTVFGNPDPEVVWFKNDKDI ELSDHFSVKVEQGKYVSMTIKGVTS EDSGKYSIHVKNKYGGEKIDVTVSV YKHGEEIPDVVLPQQAKPKLLPAAA PSPAH SEQ ID F1N0L9 Myopalladin/ MQDDSLEASTSISQLLRESYLAETRH NO: 161 (UniProtKB) Bos taurus RGNNERSRAEPSSNPFHFGGSSGAAE GGGGQDDLPDLSAFLSQEELDESVN LARLAINYDPLEKADEAQARKRFSS DQTKHSSISSFDPNFCQDNSQSPTNS KESLQETKRPQYSSEAQSKKVFLNK AADFIEELSSLFKAHSSKRIRPRACKN HKSKLESQNQVMQENSSSFPDLSERR ERSSVPIPIPADTRDNEVNHALEQQE AKRREAEQAAGEAASGDTTPGSSPSS LYYEEPLGQPPRFTQKLRSREVPEGT RVQLDCIVVGIPPPQVRWYCEGKELE NSPDIHIIQAGNLHSLTIAEAFEEDTG RYSCFASNIYGTDSTSAEIYIEGVSSS DSEGDPNKDEMNRIQKPNEVSSPPTT SAGIPSAVPQTQHVVVQPRVSTIQQC QSPTNYLQGLDGKPIIAAPVFTKMLQ NLSASEGQLVVFECRVKGAPSPKVE WYREGTLIEDSPDFRILQKKPRSMAE PEEICTLVIAEVFAEDSGCFTCTASNK YGTVSSIAQLDVRGNEDLRNNGSLH SANSTTNLALTEQQPSPPNPEPPVEQP PKPKLEGVLVNHNEPRSSSRIGLRVH FNLPEDDKGSEESSEGGVVTTHQTRP DSFQERFNGQSAKISEPSSPVKEPPPV LAKPKLDSSQLQQLHNQVLLEQHHL QNPSPSSPKEFPFNMSVLNSTTVPAV TVSSKHAKAPPSQTFSLARPKHFFPS TSTTVASVSPSSSPVFTLSSTPQAMQR TMSKESLLVTHPSAQTKSLGGASIQN EPLPTPAPTEPAQLPLTFSISSGNQFQP RCVSPAPVSPTGRIQNPVAFLSSVLPS LPAIPPTNAMGLPKSAPSMPSQGLMK KNTKSSHPVSDDYIRETKNAVILDLG KKMNFSDVRSNQQEYKISSFEQRLM NEIEFRLERTPVDESDDEIQHDEIPTG KCIAPIFDKRLKHFRVTEGSPVTFTCK IVGIPVPKVYWFKDGKQISKRNEHFK MKREGDGTCSLHINSTSSDDDGNYTI MAANPQGRISCSGHLMVQGLPIRSRL TAAGQSHRGRSRVQERDKEPLQERF FRPHFLQAPGDMVAHEGRLCRLDCK VSGLPPPELTWLLNGQPVLPDTSHK MLVRETGVHSLLIDPLTQRDAGTYT CIATNKTGQNSFSLELTVVAKEVKK APVILEKLQNSGVPEGHPVRLECRVI GMPPPVFYWKKDNETIPFTRERISMH QDTTGYVCLLIQPAKKSDAGWYTLS AKNEAGIVSCTARLDIYAQWHHQIPP PMTVRPSGSRYGSLTSKGLDIFSAFSS MESTMVYSCSSRSVVESDEL Q5E9V3 Myozenin-2/Bos MLSHNTMVKQRKQQASAIMKEIHG (UniProtKB) taurus NDVDVMHLGKKVSIPRDIMLEELSH LSNRGARLFKMRQRRSDKYTFENFQ YETKAQINHNIAMQNEKLDGINLESG SQQAPFTPPNTPDPRSPPNPENIAPGY SGPLKEIPPERFNTTAVPKYYQSPWE QAISNDPELLEALYPKFFKPEGKAEL PDYRSFNRVATPFGGFEKASKMVKF KVPDFDLLLLTDPRFMAFANPLSGRR SFNRTPKGWISENIPIVITTEPTEDNTI PESEDL SEQ ID NP_001264756.1 Myozenin 2/ MLSHSAMVKERKQQASAIMDEIQRN NO: 162 (NCBI) Gallus gallus VSPSLNLGKKVSTPRDIMVEELSTLS NRGARLFKRRQRRSDKYTYENYHY VANKPRNRGEPIHSVKMDGLSVEGIP QHAPMTPPNTPDPRSPPHPDNIAPGY SGPLKEIPPEKFNTTSVPKYYQSPWIE AIRDDPELLEALYPKLFKPEAKPELP DYRSFNRVATPFGGFERASKLVKFK VPDYNLLMLNDPRFMILANPLATRR SFNRTPKGWTSENIPVVFIQPSDSNN VPETEDL SEQ ID Q1AG08 Calsarcin 1/Sus MLSHNTMVKQRKQQASAIMKEIHG NO: 163 (UniProtKB) scrofa NDVDGMDLGKKVSIPRDIMLEELSH LSNRGARLFKMRQRRSDKYTFENFQ YESKAQINHNIAMQNGKLDGNNLES GSQQAPFTPPNTPDPRSPPNPENIAPG YSGPLKEIPPERFNTTAVPKYYQSPW EQAISNDPELLEALYPKLFKPEGKAE LPDYRSFNRVATPFGGFEKASKIVKF KVPDFELLLLTDPRFMAFANPLSGRR SFNRTPKGWISENIPIVITTEPTEDTTI PESDDL SEQ ID A8E4L9 SYNC MASPEPRRGGNGSAQAARATRPEVI NO: 164 (UniProtKB) protein/Bos SPLQEENSASLYELGTWNPEVTLSLE taurus GTLNLEDILYLGDTGDLDEALYVEET EPPEETLHIEETRMPDEALYLEEPVRP EAALYVEEPVKLEGVLYVEEPVKTA SPEQIVHGGDRVLSEAKSKPKESLQA GPSPSTEGSLSIEDLELLEGRFQQCIE AVAQLEEERDQLIHELVLLREPALQE VQQVHRDILAAYKQHAQAELERDG LREEIRLVKQKLFKVTKECVAYQYQ LECRRQDVAQFADFREALTTRAAQL SEELTQLREAYQKQKEQLRQQLEAP QSQRDGHFLQESRRLSAQFESLMAES RQGLEEEYEPQLLRLLERKEAAAKA LQKTQAEIQEMKEALRPLQAEAQKL HLQNRNLEDQITLVRQKRDEEVQQY REQLEEMEERQRQLRSGVQLQQQKN KEMEQLRVSLAEELATYKAMLPKSL EQANAPTSEAGGIETPSQGAV SEQ ID F1NM11 Syncoilin, MDAEGAGTPQAPEGTERPCLSLEEL NO: 165 (UniProtKB) intermediate GEYFQECIEAVEQLEKERDALIEELT filament QLREPALQDIRHAHQEIQAACRLLAK protein/Gallus VELERDNLRDEIRQIKQKLFKVTKEC gallus VACQYQLESRRHDLSQHAAYRDELE SQAGRLTGELSRLRESCEKEKEALRQ RLEAPPCRQDPQYLQESRRLSAEFES LVTRSRRGLEEHYEPQLLRLLERREA GTRALQELQGEVQGMKEALRPLQGE VSRLRLQNRSLEEQIVLVKQKRDEEV GQYREQVEELEDRLKELKNSVQLQQ RKNQELEELRSSLHHELSIYKGCLEIY GHLCKSEEKPDQEC SEQ ID A0A287BKP7 Syncoilin, MASPEPRRGGDGAAQAARETRAEAT NO: 166 (UniProtKB) intermediate SPLQEGNSESLYQLGTWNPEVTLSLE filament GTLNLEDILYLGDPVDLDEALYVEET protein/Sus EKPEETLHIEETRLPDEALYVEEPVKP scrofa EEMLYVEETVKPEEIVCVEQTMKPG ETTSPEPITYGGETVPSEEKPNPEESL RAKPNPSTDGSLSLEDLELLEGRFQQ CVQAVAQLEEERDQLIHELVLLREPA LQEVQQVHQDILAAYKLHAQAELER DGLREEIRLVKQKLFKVTKECVAYQ YQLECRRQDVAQFADFREVLTARAA QLSEELAQLRDAYQKQKEQLRQQLE APPSQRDGHFLQESRRLSARFENLM AESRQGLEEEYEPQLLRLLERKEAAA KALQKTQAEIQEMKEALRPLQAEAR QLHLQNRNLEDQITLVRQKRDEEVQ QYREQLEEMEERQRQLKSGVQLQQ QKNKEMEQLRISLAEELSTYKAMLP KSLERASAPTSEAGGIETQSQGAV SEQ ID A4FV66 Sarcoglycan MAAALIWISLLVGLLEGLGGTEAQQ NO: 167 (UniProtKB) alpha/Bos taurus TTLHPLVGRVFVHTLDHESFLQRPEH VFSVSAPIPITYHAHLQGHPDLPRWL RYTQRSPYQPGFLYGTATPEDRGHQI IEVTAYNRDSFNTTQQMLVLLIGDPE GPLLPYQAEFLVRSHDVEEVLPSIPAS RFLTALGGLWEPAELQLVNITSALDR GGRVPLPIEGRKEGVYIKVGSASPFS TCLKMVVSPDSHARCAQGQPPLLSC YDTLAPHFRVDWCNVSLVDKSVPEP ADEAPAPGDGILEHDPFFCPPTEATN RDFLTDALVTLLVPLLVALLLTLLLA YVMCCRREGRLKRDLATSDIQMVH HRTIRGNTEELRQMASSREVPRPLST LPMFNVHTGERLPPQVDSAQVPLILD QH SEQ ID Q0VCU7 Gamma- MVREQYTTITEGTHIERPENQAVYKI NO: 168 (UniProtKB) sarcoglycan/Bos GIYGWRKRCLYLFVLLLLIVLLVNFA taurus LTIWILRVMWFSPVGMGHLHVTADG LHLEGESEFLFPLYVKEIRSRVDSSLL LQSTQNVTMNARNTEGEVTGRLKV GPQMVEVQSQQFQIHSKDGKPLFTV DEEKVMVGTDKLRVTGPEGALFEHS VETPLVRPDPPQDLRLESPTRSLSMD APKGIHIQAPAGKIEALTQMDIVLQS SDGTVVLDAETVCLPELALGSQGPA GSSQGLYEVCVCPDGKLYLSVAGM GTTCHEHSHLCL Skeletal and smooth muscle tissue SEQ ID P12003 Metavinculin/ MPVFHTRTIESILEPVAQQISHLVIMH NO: 169 (UniProtKB) Gallus gallus EEGEVDGKAIPDLTAPVSAVQAAVS NLVRVGKETVQTTEDQILKRDMPPA FIKVENACTKLVRAAQMLQADPYSV PARDYLIDGSRGILSGTSDLLLTFDEA EVRKIIRVCKGILEYLTVAEVVETME DLVTYTKNLGPGMTKMAKMIDERQ QELTHQEHRVMLVNSMNTVKELLP VLISAMKIFVTTKNTKSQGIEEALKN RNFTVEKMSAEINEIIRVLQLTSWDE DAWASKDTEAMKRALALIDSKMNQ AKGWLRDPNAPPGDAGEQAIRQILD EAGKAGELCAGKERREILGTCKTLG QMTDQLADLRARGQGATPMAMQK AQQVSQGLDLLTAKVENAARKLEA MTNSKQAIAKKIDAAQNWLADPNG GSEGEEHIRGIMSEARKVAELCEEPK ERDDILRSLGEISALTAKLSDLRRHG KGDSPEARALAKQIATSLQNLQSKT NRAVANTRPVKAAVHLEGKIEQAQR WIDNPTVDDRGVGQAAIRGLVAEGR RLANVMMGPYRQDLLAKCDRVDQL AAQLADLAARGEGESPQARAIAAQL QDSLKDLKARMQEAMTQEVSDVFS DTTTPIKLLAVAATAPSDTPNREEVF EERAANFENHAARLGATAEKAAAV GTANKTTVEGIQATVKSARELTPQV VSAARILLRNPGNQAAYEHFETMKN QWIDNVEKMTGLVDEAIDTKSLLDA SEEAIKKDLDKCKVAMANMQPQML VAGATSIARRANRILLVAKREVENSE DPKFREAVKAASDELSKTISPMVMD AKAVAGNISDPGLQKSFLDSGYRILG AVAKVREAFQPQEPDFPPPPPDLEHL HLTDELAPPKPPLPEGEVPPPRPPPPE EKDEEFPEQKAGEAINQPMMMAAR QLHDEARKWSSKPVTVINEAAEAGV DIDEEDDADVEFSLPSDIEDDYEPELL LMPTNQPVNQPILAAAQSLHREATK WSSKGNDIIAAAKRMALLMAEMSRL VRGGSGNKRALIQCAKDIAKASDEV TRLAKEVAKQCTDKRIRTNLLQVCE RIPTISTQLKILSTVKATMLGRTNISD EESEQATEMLVHNAQNLMQSVKET VREAEAASIKIRTDAGFTLRWVRKTP WYQ SEQ ID E1BPV6 Smoothelin like MEQKAGKSSEDGATVPPAAEAPEPA NO: 170 (UniProtKB) 1/Bos taurus GSGGSAEEETAGPAESAARAGPATA GQQERAPAEDAVSAECQADGLGEV KAESQGEVELQQEDPGQGETAAAHG KTDRKDQTDSEPEGAEGDRETASAS EEQSADEKEARLGSRETVDASREVQ AEPKQASGAQEAEAGGGEAEGPQEE GGKTEEPQAEAREEAGAPAAQPDSE PGSPDEEQDQGAGAEAEDGAGGPPS SPEGWPESPTEEGGSASPEGLSPDTA ASEELGPSASDSSPSDVPQSPTEPPPS EEKKKEKAPERRVSAPTRPRGPRAQ NRKAIVDKFGGAAAGPTALFRNTKA AGAAVGGVRNMLLEWCRAMTRSYE HVDIQNFSSSWGSGMAFCALIHKFFP DAFDYAALDPAQRRHNFTLAFSTAE KLADCAQLLEVDDMVRLAVPDSKC VYTYIQELYRSLVQKGLVKTKKK Cardiac and smooth muscle tissue SEQ ID F1NHA9 PDZ and LIM MPQNVILPGPAPWGFRLSGGIDFNQP NO: 171 (UniProtKB) domain protein LIITRITPGSKASTANLCPGDIIVAING 3/Gallus gallus LSTENMTHNDAQERIKAAAHQLSLRI ERAETKLWSPQVSEDGKANPYKINL EAEPQEFKPIGTAHNRRAQPFVAAA NIDDKRQVVSSSYNSPIGLYSSGNIQ DALHGQLRSLIPNASQNDPAPTAVPQ SDVYRMLHSNQEEPSQPRQSGSFKV LQNLVSEEDGRPVGTRSVKAPVTKIP TGLPGAQKVPQCDKCGSGILGTVVK ARDKYRHPECFVCSDCNLNLKQKGY FFVEGQLYCETHARARMRPPEGYEA VTVYPKC Skeletal, cardiac, and smooth muscle tissue SEQ ID Q04205 Tensin/Gallus MDFGSVMNQAATPCSPAVNYELPSP NO: 172 (UniProtKB) gallus GQSITKQVDTPDATRSPRGGQAHCK ASRSMSVTAAMESSCELDLVYITERII AVSYPSTAEEQSFRSNLREVAHMLK SKHGDNYVLFNLSERRHDISKLHPK VLDFGWPDLHTPALEKICSICKAMDT WLNAAAHNVVVLHNKGNRGRLGV VVAAYMHYSNISASADQALDRFAM KRFYEDKVVPVGQPSQKRYIHYFSG LLSGSIKMNNKPLFLHHVIMHGIPNF ESKGGCRPFLKIYQAMQPVYTSGIYN VQGDSQTGICITIEPGLLLKGDILLKC YHKKFRSPTRDVIFRVQFHTCAVHD LDIVFGKEDLDEAFRDERFPEYGKVE FVFSYGPEKIQGMEHLENGPSVSVDY NTSDPLIRWDSYENFNIQREDSAEGT WAEPALPGKHLEKEVGHTQGPLDGS LYAKVKKKDSLHGSIGAVNTARLPL SAAPNHVEHTLSVSSDSGNSTASTKT DRTDEPGAPGAPTGHAVLSPEEKRD VDRLLVGFGLESAAPMHNHAPGPAP ARLPAGPGRHVVPAQVHVNGAGTPL LAERETDILDDELPNQDGHSVGSLGT LSSLDGTTTASEAGFHEAPRVGSLSS LPNGPASYNGAEKMLKEGLYEAEPL SNGAYPYSNQNTLMGHHLRDPLAHL RPSRSAQEHLAGYPQRQPASASPAW LQPPVPQPYLYGYDLPSAHRSQSFPA VGTAKYEANLALPQAPARSTSSREA VQRGLNSWQQQGGSRPPSQLHDGG LESHSPSLSSCSPQPSPLQPMPPHSHS MPEFPRAPSRREIEQSIEALDVLMLD LAPSVHKSQSVPSAATRQDKPAAML SSLSAQRLSGHYAQPTPQVVQPRSFG TSVGTDPLAKPYSPGPLVPAARSTAE PDYTVHEYRETYTPYSYQPVPEPRSY GSAPASILPLSASYSPAGSQQLLVSSP PSPTAPAQSQLPHKGLESYEDLSRSG EEPLNLEGLVAHRVAGVQSREKSPE ESTVPARRRTPSDSHYEKSSPEPGSPR SPTVLSPEVVSTIAANPGGRPKEPHL HSYKEAFEEMESASPSSLTSGGVRSP PGLAKTPLSALGLKPHNPADILLHPV GELEGEAGADSEEEPRSYVESVART ATTGRAGNLPAAQPVGLEVPARNGA FGNSFTVPSPVSTSSPIHSVDGASLRS YPSEGSPHGTVTPPHAVAETAYRSP MVSQTPSAHSSYQTSSPSSFQAGTLG SPYASPDYPDGRGGFQPDPQARQQP QVSVVGVHALPGSPRTLHRTVATNT PPSPGFGRRAANPAVASVPGSPGLGR HTVSPHAPPGSPSLARHQMAAVPPG SPMYGYSSPEERRPTLSRQSSASGYQ PPSTPSFPVSPAYYPGTSTPHSSSPDS AAYRQGSPTPQPALPEKRRMSAGER SNSLPNYATVNGKASSPLSSGMSSPS SGSAVAFSHTLPDFSKFSMPDISPETR ANVKFVQDTSKYWYKPDISREQAIA LLKDREPGAFIIRDSHSFRGAYGLAM KVASPPPTVMQQNKKGDITNELVRH FLIETSPRGVKLKGCPNEPNFGCLSA LVYQHSIMPLALPCKLVIPDRDPMEE KKDAASTTNSATDLLKQGAACNVLF INSVEMESLTGPQAISKAVAETLVAD PTPTATIVHFKVSAQGITLTDNQRKL FFRRHYPLNTVTFCDLDPQERKWTK TDGSGPAKLFGFVARKQGSTTDNVC HLFAELDPDQPAAAIVNFVSRVMLG SGQKR SEQ ID A0A1D5PNS4 Gelsolin/Gallus MSEVGEEQNGGRGAGLGGITAALGL NO: 173 (UniProtKB) gallus VLIPIPIPILVPVPIPIPIPIPPPQGRKGQ SRHRALAGAGPGWGGVRAVTAPGA ARCARPRAPQLRPARPERQQQPVSM VEHAEFSKAGKEPGLQIWRIEKFDLV PVPKNLYGDFFTGDSYLVLNTIRQRS GNLQYDLHFWLGDESSQDERGAAAI FTVQMDDYLQGKAVQHREVQGHES STFLGYFKSGIKYKAGGVASGFRHV VPNEVTVQRLLQVKGRRTVRATEVP VSWESFNTGDCFILDLGSNIYQWCGS NSNRQERLKATVLAKGIRDNERNGR AKVFVSEEGAEREEMLQVLGPKPSL PQGASDDTKTDTANRKLAKLYKVSN GAGNMAVSLVADENPFSQAALNTE DCFILDHGTDGKIFVWKGRSANSDE RKAALKTATDFIEKMGYPKHTQVQV LPESGETPLFKQFFKNWRDKDQTEG LGEAYISGHVAKIEKVPFDAATLHTS RAMAAQHGMEDDGSGKKQIWRIEG SEKVPVDPATYGQFYGGDSYIILYDY RHAGKQGQIIYTWQGAHSTQDEIAT SAFLTVQLDEELGGSPVQKRVVQGK EPPHLMSMFGGKPLIVYKGGTSREG GQTTPAQTRLFQVRSSTSGATRAVEL DPVASQLNSNDAFVLKTPSAAYLWV GRGSNSAELSGAQELLKVLGARPVQ VSEGREPDNFWVALGGKAPYRTSPR LKDKKMDAHPPRLFACSNKSGRFTIE EVPGDLTQDDLATDDVMILDTWDQ VFVWIGKDAQEEEKTEALKSAKRYI ETDPASRDKRTPVTLVKQGLEPPTFS GWFLGWDDDYWSVDPLQRAMADV DV SEQ ID XP_025010159.1 Dystroglycan MTVGCVPQPPFLGRTLLPVLLLAASA NO: 174 (NCBI) isoform RCHWPSEPAEVVRDWENQLEASMH X1/Gallus gallus SVLSDLRETVPAVVGIPDSSAVVGRF FRVSIPTDLIASNGEAVQVSEAGKES LPSWLHWNAESSSLEGLPLDTDKGV HYISVTTLQPFPNGSYVPQAANVFSV EVHQEDHSEPQSVRAAAQEAGDAAP FVCGAEEPVTILTVILDADLTKMTPK QRIELLNRMRSFSEVELHNMKLVPV VNNRLFDMSAFMAGPGNAKKVVEN GALLSWKLGCSLSQNSVPNISKVEAP AKEGTMSARLGYPVVGWHIANKKP HLPKRMRRQINATPTPLTAIGPPTTA AQEPPTRIVPTPTSPAIAPPTETTAPPV REPIPLPRKPTVTIRTRGPIVQTPTLGP IQPTRLVEGTGTVSVPIRPTVPGYVEP TAVITPPTTTTKKPRVSTLKPATPSTT DSSTATTRRPTRRPKTPRPTKPPSTTR STISKLTTASPPTRVRTTASGVPRPWE PNEPPKLTNHIDRVDAWEGTYFEVKI PSDTFYDKEDTTTDKLQLTLKLKEQ QMIEENSWVQFNSTSQLMYGMPDRS HVGKHEYFMYATDKGGLFAVDAFEI HVHKRPHGDKSPVKFKARLEGDHSA VANDIHKKIMLVKKLALAFGDRNSS TITVQDIAKGSIVVEWTNNTLPLEPCP REQIRTLSKKIADDSGGPSPAFSNILQ PEFKPLNVSVVGSGSCRHIQFVPVTK DGRVISEATPTLAAGKDPEKSSEDDV YLHTVIPAVVVAAILLVAGIIAMICY RKKRKGKLTIEDQATFIKKGVPIIFAD ELDDSKPPPSSSMPLILQEEKAPLPPP EYPNQSMPETTPLNQDTIGEYTPLRD EDPNAPPYQPPPPFTAPMEGKGSRPK NMTPYRSPPPYVPP SEQ ID P12003-1 Vinculin MPVFHTRTIESILEPVAQQISHLVIMH NO: 175 (UniProtKB) isoform 1/Gallus EEGEVDGKAIPDLTAPVSAVQAAVS gallus NLVRVGKETVQTTEDQILKRDMPPA FIKVENACTKLVRAAQMLQADPYSV PARDYLIDGSRGILSGTSDLLLTFDEA EVRKIIRVCKGILEYLTVAEVVETME DLVTYTKNLGPGMTKMAKMIDERQ QELTHQEHRVMLVNSMNTVKELLP VLISAMKIFVTTKNTKSQGIEEALKN RNFTVEKMSAEINEIIRVLQLTSWDE DAWASKDTEAMKRALALIDSKMNQ AKGWLRDPNAPPGDAGEQAIRQILD EAGKAGELCAGKERREILGTCKTLG QMTDQLADLRARGQGATPMAMQK AQQVSQGLDLLTAKVENAARKLEA MTNSKQAIAKKIDAAQNWLADPNG GSEGEEHIRGIMSEARKVAELCEEPK ERDDILRSLGEISALTAKLSDLRRHG KGDSPEARALAKQIATSLQNLQSKT NRAVANTRPVKAAVHLEGKIEQAQR WIDNPTVDDRGVGQAAIRGLVAEGR RLANVMMGPYRQDLLAKCDRVDQL AAQLADLAARGEGESPQARAIAAQL QDSLKDLKARMQEAMTQEVSDVFS DTTTPIKLLAVAATAPSDTPNREEVF EERAANFENHAARLGATAEKAAAV GTANKTTVEGIQATVKSARELTPQV VSAARILLRNPGNQAAYEHFETMKN QWIDNVEKMTGLVDEAIDTKSLLDA SEEAIKKDLDKCKVAMANMQPQML VAGATSIARRANRILLVAKREVENSE DPKFREAVKAASDELSKTISPMVMD AKAVAGNISDPGLQKSFLDSGYRILG AVAKVREAFQPQEPDFPPPPPDLEHL HLTDELAPPKPPLPEGEVPPPRPPPPE EKDEEFPEQKAGEAINQPMMMAAR QLHDEARKWSSKGNDIIAAAKRMAL LMAEMSRLVRGGSGNKRALIQCAK DIAKASDEVTRLAKEVAKQCTDKRI RTNLLQVCERIPTISTQLKILSTVKAT MLGRTNISDEESEQATEMLVHNAQN LMQSVKETVREAEAASIKIRTDAGFT LRWVRKTPWYQ SEQ ID E1C8N4 Supervillin/ MKRKERIARRLEGIENDTQPMLLQN NO: 176 (UniProtKB) Gallus gallus CPGSVTHRLLEEDTPRYMRATDPYS HHLGRSNEEEEASDSSVEKQPRSSRY RTETTGGNTESLYSTGNMDTHELES KAERIARYKAERRRQLAEKYGLSLD SDLDSDYSSRYSRARKDPDSVERKA LRSERHDDENKDSGSLYLSRTEVKES KSVLSESREYSSREKDGIPAKEELSNE KSDKRADDDSAIRQASDPSATLDSSV SLTLSGRESSSCNEVPISPKQSPRESLS SPKRAASPIHLQNDQPLHSNIRQSESR FEMSTSGSALSAGGDRERGPRRPRR YFTPGENRKTSERFKTQPITSAERKET DRSIMCAEIPTADDEEKLDDRAKLSV AAKRLLFREMEKSFEMKNIPKPPTRS SAVERRMRRLQDRSHTQPITSEEVVI AATLQASAHQKILAKEEIRAAKEAM GQDQSDEPDSSTLSLAEKMALFNKL SQPVSRAISTRSRGDIRHRRMNTRYQ TQPVTLGEVEQVQNESGKLTPLSSTV STSVSAMASALSALFAGDMHAKSRS DGSVSAATKDELRFHASAESSDSSGK RTQKSEQWQPSMEVLESKRASKKHE EEGKRFLAHEANEIRKYSSFEDETTH PVLEKTGDYHKEAAYSFLRKGSMEL FSSQPLFQPLERKEIDTIMQDHVEPTS ELTSTPATRTLSQTTAAASCRLQELS EQLEGKFYKNSCEMFSAGENKIQTTE DATDSSSKTMSIKERLALLKKSGEED WKSRLSKKQEYAKVSATDRSAQMQ EVEQLLKKRIADNQESQMTIEERKHL ITAREEAWKSRGKGAANDSTQFTVA GRMVKKGLASPSALTPVASPYGNRQ KSTTPVTKPLEEIEARPDMQLESDMK LDKLESFLGRLNNKVAGMQETVLTV TGKSVKEVMKLDDDETFSKFYRRVD FPSSSVPLDLDEDFDAIFDPYAPKLIS SVAEHKRAVRPMRRVQSSRNPLKML AAREDILHEYTEQRLNVAFMESKRM KVEKMSANSNFSEVALAGLASKENF SNVSLRSVNLTEQNSNNSAVPYKKL MLLQIKGRRHVQTRLVEPRASSLNS GDCFLLLTPHLCFLWVGEFANVIEKA KASELATLIQTKRELGCRASYIQTIEE GINTHTHAAKDFWKLLGGQANYQS AGRPEEDEMYEAAIIETNCIYRLVED KLIPEDDYWGKMPKCTLLQPKEVLV FDFGSEVYVWHGKEVTLAQRKVAF QLAKHLWNGTFDYSNCDINPLDPGE CNPLIPRKGQGRPDWAVFGRLTEHN ETILFKEKFLDWTELKKLNEKNSSES LHQKEESKSDSKPYDVMLMVPVPQT AVGTVLDGMNIGRGYGLVEGEDGR QFEIITASVDVWHILEFDYSRLPKQSI GQFHEGDTYVVKWKYMVSTTVGSR QKGEQQVRAVGKEKCVYFFWQGRH STVSEKGTSALMTVELDEERGAQVQ VLQGKELPCFLQCFQGGMIVHAGRR EEEEENAQSDWRLYCVRGEVPNEGN LLEVACHCSSLRSRTSMIVLNINKALI YLWHGCKAQSHTKDVGRTAANKIK EQCPLEAGLHSSSKVTIHECDEGSEP LGFWDALGRRDRKAYDCMLQDPGK FNFTPRLFSLSSSSGEFSATEYVYPSR DPTVINSMPFLQEDLYTAPQPALFLV DNHHEVYLWQGWWPVENKITGSAR IRWATDRKCAMETVLQYCKGKNVK KPPKSYLIHAGLEPLTFTNMFPSWEH REDIAEITEMDADVSNQIILVEDVLA KLCKTVYPLADLLARPLPEGVDPLK LEIYLSDEDFEVALEMTREEYNALPS WKQVNLKKAKGLF SEQ ID Q5ZL50 Profilin/Gallus MAGWQSYVDNLMCDGCCQEAAIVG NO: 177 (UniProtKB) gallus YCDAKYVWAATAGGIFQSITPVEID MIVGKDREGFFTNGLTLGAKKCSVIR DSLYVDGDCTMDIRTKSQGGEPTYN VAVGRAGRVLVFVMGKEGVHGGGL NKKAYSMAKYLRDSGF SEQ ID A5PJI6 Caveolae- MEHNGSASNADKIHQNRLSNVTEDE NO: 178 (UniProtKB) associated DQDAALTIVTVLDKVAAIVDSVQAS protein 4/Bos QKRIEERHRVMENAIKSVQIDLLKFS taurus QSHSNTGYVINKLFEKTRKVSAHIKD VKARVEKQQTHVKKVEAKQEEIMK KNKFRVVIFQEEVQCPTSLSVVKDRS LTESPEEVDEIFDTPVDLSSDEEYFVE ESRSARLKKSGKERIDNIKKAFSKEN MQKTRQNFDKKVNRIRTRIVTPERRE RLRQSGERLRQSGERLKQSGERFKKS ISNAAPSREAFKMRSLRKTKDRAVA EGPEEVREMGVDIIARGEALGPISEL YPEALSETDPEEASATHPPQEGGEVS TPEPLKVTFKPQVKVEDDESLLLDLK Q SEQ ID Q62234 Myomesin- MSLPFYQRSHQHYDLSYRNKDLRTT NO: 179 (UniProtKB) 1/Mus musculus MSHYQQEKKRSAVYTHGSTAYSSRS LAARRQESEAFSQASATSYQQQASQ TYSLGASSSSRHSQGSEVSRKTASAY DYGYSHGLTDSSLLLEDYSSKLSPQT KRAKRSLLSGEETGSLPGNYLVPIYS GRQVHISGIRDSEEERIKEAAAYIAQ KTLLASEEAIAASKQSTASKQSATSK RTTSTLQREETFEKKSRNIAIREKAEE LSLKKTLEETQTYHGKLNEDHLLHA PEFIIKPRSHTVWEKENVKLHCSVAG WPEPRLTWYKNQVPINVHANPGKYI IESRYGMHTLEISKCDFEDTAQYRAS AMNVQGELSAYASVVVKRYKGELD ESLLRGGVSMPLSFAVTPYGYASKFE IHFDDKFDVSFGREGETMSLGCRVVI TPEIKHFQPEVQWYRNGAPVSPSKW VQPHWSGDRATLTFSHLNKEDEGLY TIRVRMGEYYEQYSAYVFVRDADAE IEGAPAAPLDVVSLDANKDYIIISWK QPAVDGGSPILGYFIDKCEVGTDTWS QCNDTPVKFARFPVTGLIEGRSYIFR VRAVNKTGIGLPSRVSEPVAALDPAE KARLKSHPSAPWTGQIIVTEEEPTEG VIPGPPTDLSVTEATRSYVVLSWKPP GQRGHEGIMYFVEKCDVGAENWQR VNTELPVKSPRFALFDLVEGKSYRFR VRCSNSAGVGEPSETTEVTVVGDKL DIPKAPGKIIPSRNTDTSVVVSWEESR DAKELVGYYIEASVVGSGKWEPCNN NPVKGSRFTCHGLTTAQSYIFRVRAV NAAGLSEYSQDSEAIEVKAAIGGGVS PDVWPQLSDTPGGLTDSRGGMNGAS PPTSQKDALLGSNPNKPSPPSSPSSRG QKEVSTVSESVQEPLSSPPQEAAPEE EQSQSEPPKKKKDPVAVPSAPYDITC LESFRDSMVLGWKQPDTTGGAEITG YYVNYREVVGEVPGKWREANIKAV SDAAYKISNLKENTLYQFQVSAMNI AGLGAPSTVSECFKCEEWTIAVPGPP HSVKLSEVRKNSLVLQWKPPVYSGR TPVTGYFVDLKEASAKDDQWRGLN EAAIVNKYLRVQGLKEGTSYVFRVR AVNQAGVGKPSDLAGPVVAETRPGT KEVVVSVDDDGVISLNFECDQMTPK SEFVWSKDYVPTEDSPRLEVENKGD KTKMTFKDLGTDDLGTYSCDVTDTD GIASSYLIDEEEMKRLLALSQEHKFP TVPTKSELAVEILEKGQVRFWMQAE KLSSNAKVSYIFNEKEIFEGPKYKMH IDRNTGIIEMFMEKLQDEDEGTYTFQ IQDGKATGHSTLVLIGDVYKKLQKE AEFQRQEWIRKQGPHFAEYLSWEVT GECNVLLKCKVANIKKETHIVWYKD EREISVDEKHDFKDGICTLLITEFSKK DAGFYEVILKDDRGKDKSRLKLVDE AFQDLMTEVCKKIALSATDLKIQSTA EGIRLYSFVCYYLDDLKVNWSHNGT GIKYTDRVKSGVTGEQIWLQINEPTP NDKGKYVMELFDGKTGHQKTVDLS GQAFDEAFAEFQRLKQAAIAEKNRA RVLGGLPDVVTIQEGKALNLTCNVW GDPPPEVSWLKNEKPLTSDDHCSLKF EAGKTAFFTISGVSTADSGKYGLVV KNKYGSETSDFTVSVFIPEEELRKGA MEPPKGNQKSK SEQ ID F1MKE9 Spectrin beta QPADMTSATEFENVGNQPPYSRINA NO: 180 (UniProtKB) chain/Bos taurus RWDGPDDELDNDNSSARLFERSRIK ALADEREVVQKKTFTKWANSHLVH VSCRITDLYKDLRDGRMLIKLLEVLS GEMLPKPTKGKMRIHCLENVDKALQ FLREQRVHLENMGSHDIVDGNHRLV LGLIWTIILRFQIQDIVVQTQEGRETR SAKDALLLWCQMKTAGYPHVNVTN FTSSWKDGLAFNALIHKHRPDLIDFD KLKDSNARHNLEHAFEVAERELGIIP LLDPEDVFTENPDEKSIITYVVAFYH YFSKMKVLAVEGKRVGKVIDHAIET EKMIEKYSGLASDLLTWIEQTITILNS RKFANSLAGVQQQLQAFSTYRTVEK PPKFQEKGNLEVLLFTIQSRMRANNQ KVYTPHDGKLVSDINRAWESLEEAE YRRELALRDELIRQEKLEQLARRFDR KAAMRETWLNENQRLVAQDNFGYD LAAVEAAKKKHEAIETDTAAYEERV RALEDLAQELERENYHDQKRIMARK DNILRLWDYLQELLQARRQRLEKTL DLQKLFQDMLHSIDWMDGIKAHLLS AEFGKHLLEVEDLLQKHKLMEADIA IQGDKVKAITTATLQFTEGPGYQPCD PQVIQDRVSHLEQCFEELSNTAAGRK AQLEQSKQLCKFFWEMDEAESWIKE KEQIYSSLDYGKDLTSVLILQRKHKA FEDELRGLDAHLDQIFREAEGMVAR KQFGHERIEVRIKKVSDQWNELKDL AAFCKKNLQDTENFFQFQGDADDLK AWLQDAHRLLSGEDVGHDEGATRA LGKKHKDFLEELEESRGVMEHLEQQ AQAFPQGFQDSPDVTNRLQALRDLY QQVVAQADMRQQRLQDALDLYTVF GETDACELWMGEKGKWLAQMEIPD TLEDLEVVQHRFDILDQEMKTLMTQ IDGVNLAANSLVESNHPRSAEVKKY QDRLNTRWQDFQTMVSERREAVDS ALRVHNYCVDCEETGKWIADKTKV VESTKDLGRDLAGVIAIQRKLSGLER DVAAIQARLGTLERESQQLMASHPE LKEDIEQRQAYVEELWQGLMQALKS QEASLGEASQLQAFLQDLDAFQAWL STTQKDVASKDMPESLPEAEQLLQQ HAAIKDDIDRHQESYQKVRVSGEKV THGQTDPEYLLLGQRLDGLEKGWD ALSRMWESRSQALTQCLGFQEFQKD AKQAEAILSNQEYTLAHLEPPDSLEA AEAGIRKFEDFLLSMENNRDKVLSPV DSGNKLVAEGNLYSDKIKEKVQLIE DRHRKNNEKAQEASDLLKDNLELQ NFLQNCQELTLWINDKLLTSQDVSY DEARNLHNKWLKHQAFVAELASHQ GWLENIDAEGKQLMEEKPQFAVLVS QKLEALHQLWDELQANTQEMAQHL SAARSSDLRSQTQADLNKWIRAMED QLQSDDLGKDLTSVNRMLAKLKRV EDQVNVRKEELQELFAQMPSLGEEA GDEALSIEKRFLDLLEPLGRRKKQLE SSRAKLQISRDLEDETLWVEERLPLA QSTDYGTNLQTVQLFMKKNQTLQN EILGHTPRVEDVLHRGQQLVAAAEID CQDVEERLGKLQGSWDTLQEAAAS RLQHLREASEAQQYYLDAGEAEAWI GEQEFYVFSDENPQDEESAIVMLKR HLRQQRAVDEYGRNIKQLAGRAQG LLSAGHPEGEQIIRLQGQVDKQYAGL KDMAEERRRKLENMYHLFQLKREV DDLEQWITEKDTVASSSEMGQDFDH VTVLRDKFRDFARETGAIGQERVDN VNAIIERLIDAGHSEAATIAEWKDGL NEMWADLLELIDTRMQLLAASYDL QRYFYTSSEILGLIGEKHRELPEDVGL DASTAESFHRTHTAFERELHLLGEQV QQFQDVAARLQTAYAGEKADIIQNK EQEVSAAWQALLDACAGRRTQLVD TADKFRFFSMARDLLSWMESIVRQIE TQEKPRDVSSVELLMKYHQGIQAEIE TRSKNFSTCLELGESLLQRQHQASEE IREKREQVVSRREEMQEKWEARWE RLRMLLEVCQFFRDASVAEAWLIAQ EPYLASRDYGHTVDSVEKLIKRHEAF EKSAASWEGRFAALEKPTTLELKER QTPERTEEEPGLQEEEGETAGEAPRG PHQATTERTSPGEEERPWPQDLQPPP PPGPHEDGQEEKSSTDERPATEPLFK VLDTPLSEGDEPTTLPAQRDRGHSVQ MEGFLGRKHDLEGPNKKASNRSWN NLYCVLRNSELAFYKDAKNLALGVP YHGEEPLALRHAICEIAANYKKKKH VFKLRLSNGSEWLFHGKDEEEMLSW LQGVSTAINESQSIRVKAQSLPLPPIT GPEASLGKKDKEKRFSFFPKKK SEQ ID E1BIS6 Synemin/Bos MLSWRLHTGPEKAELQELNARLYD NO: 181 (UniProtKB) taurus YVCRVRELERENLLLEEELRSRRGQE GLWAEAEARCTEEARGLRRQLDELS WATALAQGERDALRRELRELQLLGE ETRAARGRLDAELDSQRRELQEELA ARAALEALLGRLQAERRGLDAARER DVRELRARAAGLTLRYRGRVAGPA APPLRLRELHEDCALLVTEAWRETV QRYEDEVRELEEALRRGRESRREAE EETRLCAQEAEALRREVLELEQLRAL LEEELLRVREASELQAEERQREIAYL EDEKAALTLAMADRLRDYQDLVQV KTGLSLEVATYRALLEGESNPEILIVE CIENMPQEFREKSYQYTTSVLQKENE RNLFPRQKAPPASFRQSLAPRSQTPV RSDARRDVLGSGYSSFTTAWQENAY QKTASGQTNFRTFSPTPGLLRNTEAQ LKTFPERPRTEGTKAPPASSAKESAA AAEPYQERRAEEAAAASEGTWSNER TVIWGKKTEAKATKEQERNRPGTIQ TKREEKMFDSKEKASEERNLRWEEL TKLDKEARKRESEQMREKAREKESL KEESVKGREIPIRLEASQDSTAKVAP QDLQTPLKEDAGDGTGRGVETREAG FTLGASDTTASLKGGSMTETIAENIV SSILKQFTHSPEAEASAESFPDTKVTY VDRKEVPGDRKAKTEIVVESKLTEEI DISDEAGLDYLLSKDAKEVELKGKS AERLIGDVIRLGLKGKEGRAKVVNV EIIEEPMSYVSSEKEDEFSTPFEVEEID DVSPSSRGLVEEREEAVYGESDVTCS GDAGQEARRPQESVTHVEEVTEAGD LEGEQSYFVSTPDEHPGAPEREEGSV YGQIHIEEESTIRYSWQDEIVPGSRRR VRRDDVPGEKVVKPVDVPEVPLEGD TASAPWKEQTRSGEFHAKPIVIEKEI KIPHEFHTSIKEGSKEPRHQLVEVIEQ LEENLPERMKEELSALTREGQGGPA GVSFDVKKVQSAGSGAVTLVAEVNL SQTVDADQLDLEELSKDEAGEIEKA VESVVRDSLARRRSPGPGSPEAEAGG GFRRWATQELYSSSAGEADAGLASP PGPVSATVEVTSPTGFVHAHVLEDVS QSGGRVQIASPGMWRTEQVSAEGRA AQAVEVSAEGQASWAEGSAGTSRSA RLITLGARQSPVSTEVIFPGPDAACPE AGGTEEPGPAELPTEPPRFGRHSPFGS QQFYAQREVIFQAPVSGVGKAGDSS QAEESAGTQTSVKHLQLGTREGLTE QTQLTAPLSGAVELGVREASVLMEA WSGDGTSIRHVTIGPQRHQATEPIVP PPLEFSDSESSTHREGSADETLATSSY TVGRNILVTEKSTFQRAVSESPQEAS AEDMSGNEVTSGVSRSFRHIQLGPAE AKTSEHIVFHGPISKTFALAGSVDSPE VSEVADSGRTLRHVALGPKETSFTFQ MDVSNVEATSRWTQEARVLFPAGTE AEAPSVSEPGAWRDASSRNDLAAGV SFQGSAGDRHQAPGERGKEQAEFDK TVQLQRMVDQRSVISDEKKVALLYL DSQEEENEGHWF SEQ ID E1BF59 Plectin/Bos MVAGMFMPLDQLRAIYEVLFREGV NO: 182 (UniProtKB) taurus MVAKKDRRPRSLHPHVPGVTNLQV MRAMASLRARGLVRETFAWRHFYW YLTNEGIAHLRQYLHLPPEIVPASLQ RVRRPVAMVMPARRTPHVQAVQGP LGCPPKRGPPPTEDPAREERCVYRRK EPEEGAPEPPVVPAATPGTLARQGLE PAPPTDERDRVQKKTFTKWVNKHLI KAQRHISDLYEDLRDGHNLISLLEVL SGDSLPREKGRMRFHKLQNVQIALD YLRHRQVKLVNIRNDDIADGNPKLT LGLIWTIILHFQISDIQVSGQSEDMTA KEKLLLWSQRMVEGYQGLRCDNFT SSWRDGRLFNAIIHRHKPMLIDMNK VYRQTNLENLDQAFSVAERDLGVTR LLDPEDVDVPQPDEKSIITYVSSLYD AMPRVPDVQDGVKANELQLRWQEY RELVLLLLQWIRAHTAAFEERRFPSS FEEIEILWCQFLKFKETELPAKEADK NRSKGIYQSLEGAVQAGQLKVPPGY HPLDVEKEWGKLHVAILEREKQLRS EFERLERLQRIVSKLQMEAGLCEEQL NQADALLQSQDVRLLAAGKAPQRA GEVERDLDKADGMIRLLFNDVQALK DGRHPQGEQMYRRVYRLHERLVAIR TEYNLRLRGTPRHPELEDSTLRYLQD LLAWVEENQRRLDGAEWGVDLPSV EAQLGSHRGLHQSVEEFRAKIERART DEGQLSPATRGAYRDCLGRLDLQYA KLLNSSKARLRSLESLHGFVAAATKE LMWLSEKEEEEVGFDWSERNSNMA AKKEAYSALMRELELKEKKIKEIQST GDRLLREDHPARPTVESFQAALQTQ WSWMLQLCCCIEAHLKENTAYFQFF SDVREAEEQLRKLQETLHRKYTCDR SITVTRLEDLLQDAQDEKDQLNEYR GHLSGLAKRAKAIVQLTPRNPTQPTR GRVPLLAVCDYKQVEATVHKGDEC QMLGPAQPFHWKVLSGSGSEAAVPS VCFLVPPPNQEALEAVARLEAQHQA LVTLWHQLHTDMKSLLAWQSLSRD VQLIRSWSLVTFRTLKPEEQRQALRS LELHYQAFLRDSQDAGGFGPEDRLQ AEREYGSCSRHYQQLLQSLEQGKCG QCGXLDKEPARECAQRIAEQQKAQA EVEGLGKGVARLSAEAEKVLALPEP SPAAPTLRSELELTLGKLEQVRSLSAI YLEKLKTISLVIRSTQGAEEALKAHE EQLKEAQAVPAALPELEATKAAMK KLRAQAEAQQPVFDALRDELRGAQE VGERLQQRHGERDVEVERWRERVT QLLERWQAVLAQTDVRQRELEQLG RQLRYYRESADPLGAWLQDARRRQ EQIQAVPLADSQAVREQLRQEKALL EEIERHAEKVEECQRFAKQYINAIKD YELQLVTYKAQLEPVASPAKKPKVQ SGSESVIQEYVDLRTRYSELSTLTSQ YIRFISETLRRMEEEERLAEQQRAEE RERLAEVEAALEKQRQLAEAHAQA KAQAEREAQELQRRMQEEVARREE VVVDAQQQKRSIQEELQQLRQSSEA EIQAKARQVEAAERSRLRIEEEIRVV RLQLETTERQRGGAEGELQALRARA EEAEAQKRQAQEEAERLRRQVQDET QRKRQAEAELGLRVKAEAEAAREK QRALQALEELRLQAEEAERRLRQAE AERARQVQVALETAQRSAQAELQSK HASFAEKTAQLERTLEEEHVTVVQL REEATRREQQQAEAERAREEAEREL ERWQLKANEALRLRLQAEEVAQQK SLAQAEAEKQKEAAEREARRRGKAE EQAVRQRELAEQELERQRQLAEGTA QQRLAAEQELIRLRAETEQGEQQRQ LLEEELARLQSEAAAATQKRQELEA ELAKVRAEMEVLLASKARAEEESRS SSEKSKQRLEAEAGRFRELAEEAARL RALAEEAKRQRQLAEEDAARQRAE AERVLSEKLAAISEATRLKTEAEIAL KEKEAENERLRRLAEDEAFQRRRLE EQAAQHKADIEERLAQLRKASESELE RQKGLVEDTLRQRRQVEEEILALKA SFEKAAAGKAELELELGRIRGNAEDT LRSKEQAEQEAARQRQLAAEEERRR REAEERVQKSLAAEEEAARQRKAAL EEVERLKAKVEEARRLRERAEQESA RQLQLAQEAAQKRLQAEEKAHAFA VQQKEQELQQTLQQEQSMLERLRGE AEAARRAAEEAEEARERAEGEAAQS RQRVEEAERLKQAAEEQAQAQAQA QAAAEKLRKEAEQEAARRAQAEQA ALRQKQAADAEMEKHKKFAEQTLR QKAQVEQELTALRLKLEETDHQKSIL DQELQRLKAEVTEAARQRSQVEEEL FSVRVQMEELGKLKARIEAENRALIL RDKDNTQRLLQEEAEKMKQVAEEA ARLSVAAQEAARLRQLAEEDLAQQR ALAEKMLKEKMQAVQEATRLKAEA ELLQQQKELAQEQARRLQEDKEQM AQQLAQETQGFQRTLETERQRQLEM SAEAERLRLRVAEMSRAQARAEEDA QRFRKQAEEISAKLHRTELATQEKVT LVQTLETQRQQSDRDADRLREAIAE LEREKDKLKKEAELLQLKSEEMQTV QQEQLLQETQALQQSFLSEKDSLLQR ERFIEEEKAKLERLFQDEVAKAQKLR EEQQRQQQQMQQEKQQLLASMEEA RRRQREAEEGVRRKQEELQLLEQQR QQQEQLLAEENRRLRERLEHLEEEH RAALAHSEEITAAQAAATRALPNGQ DATDGPAAEPEHAFEGLRQKVPAQQ LQEAGILSTEEVQRLVQGHTTVAELT QREDVRRYLQGHSSIAGLLLKPANE KLTIYAALRRQLLSPGTAVILLEAQA ASGFLLDPVRNRRLTVNEAVKEGVV GPELHHKLLSAERAVTGYKDPYTGE QISLFQAMKKDLIVREHGIRLLEAQI ATGGVIDPVHSHRVPVDVAYQRGYF DEEMNRVLQDPSDDTKGFFDPNTHE NLTYLQLLERCVEDPETGLRLLPLTD QAAKGGELVYTDSEARDVFEKATVS APFGKFQGKTVTIWELINSEYFTAEQ RRDLLRQFRTGKVTVEKIIKIVITVIE EHEQKGQLCFQGLRALVPAAELLES GVIDWDLFRQLQLGERSVQEVAEVE AVRRALRGSGVIAGVWLEEARQKLS IYEALKKELLQPEAAVALLEAQAGT GHVIDPATSARLTVDEAVRAGLVGP ELHEKLLSAEKAVTGYKDPYSGQSV SLFQALKKGLIPREQGLRLLDAQLST GGIVDPSKSHRLPLDVACARGYLDK ETSTALTAPRDDAKTYYNPRTWEPA TYSQLQQQCRPDPLTGLSLLPLSEEA ARARQQELYSEVQAREAFQKATVEV PVGSFQGRAVTIWELINSEYFTAEQR QELLRQFRTGKVTVEKIIKIIITIVEEV ETTRRERLSFSGLRAPVPASELLASGI LSSSQFEQLKDGKTSVKDLSELDSVR TLLQGSGCLAGIYLEESKEKVTIYEA MRRGLLRPSTAILLLEAQAATGFLVD PVRNQRLYVHEAVKAGVVGPELHE KLLSAEKAVTGYKDPYSGSTISLFQA MKKGLVVREHGIRLLEAQIATGGIID PVHSHRVPVDVAYQRGYFDKEMNR VLEDPSDDTKGFFDPNTRENLTYRQL LERCVEDPETGLRLLPLKGPEKAEVV ETTRVYTEEETRRAFEETQIDIPGGGS HGGSTMSLWEVMQSDLIPEEQRAQL MADFQAGRVTKERMIIIIIEIIEKTEIV RQQNLASYDYVRRRLTAEDLHEARV ISRESYSLLREGTRSLREVLEAESAW RYLYGTGCVAGVYLPGSRQTLTIYQ ALKKGLLSAEVARLLLEAQAATGFL LDPVKGDRLTVDEAVRKGLVGPELH DRLLSAERAVTGYRDPYTEQTISLFQ AMKKDLIPAEEALRLLDAQLATGGI VDPRLGFHLPLEVAYQRGYLNKDTH DQLSEPSEVRSYIDPSTDERLSYTQLL RRCRRDEASGLFLLPLSDARKLTFRG LRKQITVEELVRSHVMDEATAQRLQ EGLTSIEEVSKNLQKFLEGTSSIAGVL VDATKERLSVYQAMKKGIIRPGTAF ELLEAQAATGYVIDPIKGLKLTVEEA VRMGIVGPEFKDKLLSAERAVTGYK DPYSGKLISLFQAMKKGLILKDHGIR LLEAQIATGGIIDPEESHRLPVDVAY QRGLFDEEMNEILTDPSDDTKGFFDP NTEENLTYLQLMERCVTDPQTGLRL LPLKEKKRERKTSSKSSVRKRRVVIV DPETGKEMSVYEAYRKGLIDHQTYL ELSEQECEWEEITISSSDGVVKSMIID RRSGRQYDIDEAIAKSLIDRSALDQY RAGTLSITEFADMLSGNAGGFRSRSS SVGSSSSYPISPAVSRTQLASWSDPTE ETGPVAGILDTETLEKVSITEAMHRN LVDNITGQRLLEAQACTGGIIDPNTG ERFPVTEAVNKGLVDKIMVDRINLA QKAFCGFEDPRTKTKMSAAQALKK GWLYYEAGQRFLEVQYLTGGLIEPD TPGRVPLDEALQRGTVDARTAQKLR DVSAYSKYLTCPKTKLKISYKDALD RSMVEEGTGLRLLEAATQSSKGYYS PYSVSGSGSTTGSRSGSRTGSRAGSR RGSFDATGSGFSMTFSSSSYSSSGYG RRYASGPTSSLGGPESTAA SEQ ID Q63258-2 Integrin alpha-7 MARIPRCDFLGLPGICYLLSFLLAGLL NO: 183 (UniProtKB) Isoform Alpha- LPRASAFNLDVMGAIRKEGEPGSLFG 7X1A/Rattus FSVALHRQLQPRPQSWLLVGAPQAL norvegicus ALPGQQANRTGGLFACPLSLEETDC YRVDIDRGANVQKESKENQWLGVS VRSQGAGGKVVTCAHRYESRQRVD QVLETRDVIGRCFVLSQDLAIRDELD GGEWKFCEGRPQGHEQFGFCQQGT AATFSPDSHYLIFGAPGTYNWKGLLF VTNIDSSDPDQLVYKTLDPADRLTGP AGDLTLNSYLGFSIDSGKGLMRSEEL SFVAGAPRANHKGAVVILRKDSASR LIPEVVLSGERLTSGFGYSLAVTDLN SDGWADLIVGAPYFFERQEELGGAV YVYMNQGGHWADISPLRLCGSPDS MFGISLAVLGDLNQDGFPDIAVGAPF DGDGKVFIYHGSSLGVVTKPSQVLE GEAVGIKSFGYSLSGGLDVDGNHYP DLLVGSLADTAALFRARPVLHVSQEI FIDPRAIDLEQPNCADGRLVCVHVKV CFSYVAVPSSYSPIVVLDYVLDGDTD RRLRGQAPRVTFPGRGPDDLKHQSS GTVSLKHQHDRVCGDTVFQLQENV KDKLRAIVVTLSYGLQTPRLRRQAP DQGLPLVAPILNAHQPSTQRTEIHFL KQGCGDDKICQSNLQLAQAQFCSRIS DTEFQALPMDLDGTALFALSGQPFIG LELTVTNLPSDPARPQADGDDAHEA QLLATLPASLRYSGVRTLDSVEKPLC LSNENASHVECELGNPMKRGTQVTF YLILSTSGITIETTELKVELLLATISEQ DLHPVSVRAHVFIELPLSISGVATPQQ LFFSGKVKGESAMRSERDVGSKVKY EVTVSNQGQSLNTLGSAFLNIMWPH EIANGKWLLYPMRVELEGGQGPEKK GICSPRPNILHLDVDSRDRRRRELGQ PEPQEPPEKVEPSTSWWPVSSAEKRN VTLDCAQGTAKCVVFSCPLYSFDRA AVLHVWGRLWNSTFLEEYMSVKSL EVIVRANITVKSSIKNLLLRDASTVIP VMVYLDPVAVVAEGVPWWVILLAV LAGLLVLALLVLLLWKCGFFRRNSP SSSFPANYHRAHLAVQPSAMEAGGP GTVGWDSSSGRSTLRPLYPSTTQ SEQ ID A0A140T8D2 Integrin MNLQLIFWIGLISSVCCVFGQADENR NO: 184 (UniProtKB) beta/Bos taurus CLKANAKSCGECIQAGPNCGWCTNS TFLQEGMPTSARCDDLEALKKKGCH PNDIENPRGSKDIKKNKNVTNRSKGT AEKLQPEDITQIQPQQLVLQLRSGEP QTFTLKFKRAEDYPIDLYYLMDLSYS MKDDLENVKSLGTDLMNEMRRITSD FRIGFGSFVEKTVMPYISTTPAKLRNP CTNEQNCTSPFSYKNVLSLTDKGEVF NELVGKQRISGNLDSPEGGFDAIMQ VAVCGSLIGWRNVTRLLVFSTDAGF HFAGDGKLGGIVLPNDGQCHLENDV YTMSHYYDYPSIAHLVQKLSENNIQT IFAVTEEFQPVYKELKNLIPKSAVGT LSANSSNVIQLIIDAYNSLSSEVILENS KLPEGVTINYKSYCKNGVNGTGENG RKCSNISIGDEVQFEISITANKCPNKN SETIKIKPLGFTEEVEIILQFICECECQ GEGIPGSPKCHDGNGTFECGACRCN EGRVGRHCECSTDEVNSEDMDAYC RKENSSEICSNNGECVCGQCVCRKR DNTNEIYSGKFCECDNFNCDRSNGLI CGGNGVCKCRVCECNPNYTGSACD CSLDTTSCMAVNGQICNGRGVCECG ACKCTDPKFQGPTCEMCQTCLGVCA EHKECVQCRAFNKGEKKDTCAQECS HFNITKVENRDKLPQPGQVDPLSHC KEKDVDDCWFYFTYSVNGNNEATV HVVETPECPTGPDIIPIVAGVVAGIVL IGLALLLIWKLLMIIHDRREFAKFEKE KMNAKWDTQENPIYKSPINNFKNPN YGRKAGL SEQ ID P07228 Integrin beta- MAETNLTLLTWAGILCCLIWSGSAQ NO: 185 (UniProtKB) 1/Gallus gallus QGGSDCIKANAKSCGECIQAGPNCG WCKKTDFLQEGEPTSARCDDLAALK SKGCPEQDIENPRGSKRVLEDREVTN RKIGAAEKLKPEAITQIQPQKLVLQL RVGEPQTFSLKFKRAEDYPIDLYYLM DLSYSMKDDLENVKSLGTALMREM EKITSDFRIGFGSFVEKTVMPYISTTP AKLRNPCTGDQNCTSPFSYKNVLSLT SEGNKFNELVGKQHISGNLDSPEGGF DAIMQVAVCGDQIGWRNVTRLLVFS TDAGFHFAGDGKLGGIVLPNDGKCH LENNMYTMSHYYDYPSIAHLVQKLS ENNIQTIFAVTEEFQAVYKELKNLIPK SAVGTLSSNSSNVIQLIIDAYNSLSSE VILENSKLPKEVTISYKSYCKNGVND TQEDGRKCSNISIGDEVRFEINVTAN ECPKKGQNETIKIKPLGFTEEVEIHLQ FICDCLCQSEGEPNSPACHDGNGTFE CGACRCNEGRIGRLCECSTDEVNSED MDAYCRRENSTEICSNNGECICGQC VCKKRENTNEVYSGKYCECDNFNC DRSNGLICGGNGICKCRVCECFPNFT GSACDCSLDTTPCMAGNGQICNGRG TCECGTCNCTDPKFQGPTCEMCQTC LGVCAEHKDCVQCRAFEKGEKKETC SQECMHFNMTRVESRGKLPQPVHPD PLSHCKEKDVGDCWFYFTYSVNSNG EASVHVVETPECPSGPDIIPIVAGVVA GIVLIGLALLLIWKLLMIIHDRREFAK FEKEKMNAKWDTGENPIYKSAVTTV VNPKYEGK SEQ ID Q9GLP0 Integrin beta- MNLQLIFWIGLISSVCYVFGQADENR NO: 186 (UniProtKB) 1/Sus scrofa CLKANAKSCGECIQAGPNCGWCTNS TFLQEGMPTSARCDDLEALRKKGCH PDDIENPRGSKNIKKNKNVTNRSKGT AEKLQPEDITQIQPQQLVLQLRSGEP QTFTLKFKRAEDYPIDLYYLMDLSYS MKDDLENVKSLGTDLMNEMRRITSD FRIGFGSFVEKTVMPYISTTPAKLRNP CTSEQNCTSPFSYKNVLSLTDKGEVF NELVGKQRISGNLDSPEGGFDAIMQ VAVCGSLIGWRNVTRLLVFSTDAGF HFAGDGKLGGIVLPNDGHCHLENDV YTMSHYYDYPSIAHLVQKLSENNIQT IFAVTEEFQPVYKELKNLIPKSAVGT LSANSSNVIQLIIDAYNSLSSEVILENS KLPEGVTINYKSYCKNGVNGTGENG RKCSNISIGDEVQFEISITANKCPNKN SETIKIKPLGFTEEVEIILQFICECECQS EGIPSSPKCHDGNGTFECGACRCNEG RVGRHCECSTDEVNSEDMDAYCRK ENSSEICTNNGECVCGQCVCRKRDN TNEIYSGKFCECDNFNCDRSNGLICG GNGVCKCRVCECNPNYTGSACDCSL DTTSCMAVNGQICNGRGVCECGVC KCTDPKFQGPTCEMCQTCLGVCAEH KECVQCRAFNKGEKKDTCAQECSHF NITKVENRDKLPQPGQVDPLSHCKE KDVDDCWFYFTYSVNGNNEATVHV VETPECPTGPDIIPIVAGVVAGIVLIGL ALLLIWKLLMIIHDRREFAKFEKEKM NAKWDTGENPIYKSAVTTVVNPKYE GK SEQ ID F1MX12 Ankyrin repeat WQKHLAGRGWGPWHIKPPGPAEAG NO: 187 (UniProtKB) domain 2/Bos CDGTMADSEEVQRATALIEERLAQE taurus EENEKLRGTTHQKLPMEMLVLEDEK HHRPESPSLQKVKGQERVRKTSLDL RREIIDVGGIQNLIQLRKKRKQKKRE ALAASQEPPPEPEEITGPVDEETFLKA AVEGKMKVIEKFLADGGSPDTCDQF RRTALHRASLEGHMEILEKLLESGAT VDFQDRLDCTAMHWACRGGHLEVV RLLQSRGADTNVRDKLLSTPLHVAV RTGQVEIVEHFLSLGLDINAKDREGD SALHDAVRLNRYKIIKLLLLHGADM MSKNLAGKTPTDLVQLWQADTRHA LENPEPGSEQNGLEGSTESGRETPQP VAAE SEQ ID F1NG08 Ankyrin MAAPAPPAPGGGTSPPAGPPRLRQSD NO: 188 (UniProtKB) 2/Gallus gallus SNASFLRAARAGNLDKVVEYLKSGI DINTCNQNGLNALHLAAKEGHVGLV QELLERGSAVDSATKKGNTALHIASL AGQAEVVKVLVKEGANINAQSQNG FTPLYMAAQENHIEVVKYLLENGAN QSTATEDGFTPLAVALQQGHNQAVA ILLENDTKGKVRLPALHIAARKDDTK SAALLLQNDHNADVQSKMMVNRTT ESGFTPLHIAAHYGNVNVATLLLNR GAAVDFTARNGITPLHVASKRGNTN MVKLLLDRGGQIDAKTRDGLTPLHC AARSGHDQVVELLLERGAPLLARTK NGLSPLHMAAQGDHVECVKHLLQH KAPVDDVTLDYLTALHVAAHCGHY RVTKLLLDKRANPNARALNGFTPLHI ACKKNRIKVMELLVKYGASIQAITES GLTPIHVAAFMGHLNIVLLLLQNGAS PDVTNIRGETALHMAARAGQVEVVR CLLRNGALVDARAREEQTPLHIASRL GKTEIVQLLLQHMAHPDAATTNGYT PLHISAREGQVDVASVLLEAGASHS MSTKKGFTPLHVAAKYGSLEVAKLL LQRRASPDSAGKNGLTPLHVAAHYD NQKVALLLLEKGASPHATAKNGYTP LHIAAKKNQMQIATTLLNYGAETNIL TKQGVTPLHLASQGGHTDMVTLLLE KGSNIHVATKTGLTSLHLAAQEDKV NVAEILTKHGANQDAQTKLGYTPLI VACHYGNIKMVNFLLKQGANVNAK TKNGYTPLHQAAQQGHTHIINVLLQ HGAKPNAITTNGNTALAIARRLGYIS VVDTLKVVTEEITTTTTTVTEKHKLN VPETMTEVLDVSDEEAFKHSDDERF SDGEVYNGTGVISRNSWSDDTMTGD GGEYLRPEDLKELGDDSLPSSQFLDG MNYLRYSLEGGRSDSLRSFSSDRSHT LSHASYLRDSAMIDDTVVIPSQQVTT LAKEAERNSYRLSWGPENLDNVALS SSPIHSGCSSPCLDHDNSSFLVSFMVD ARGGAMRGCRHNGLRIIIPPRKCTAP TRVTCRLVKRHRLATMPPMVEGEGL ASRLIEVGPSGAQFLGPVIVEIPHFAA LRGKERELVILRSENGDSWKEHFCE YTEDELNEILNGMDEVLDTPEELEKK RICRIITRDFPQYFAVVSRIKQDSNLIG PEGGVLSSTVVPQVQAVFPEGALTK RIRVGLQAQPMHTELIKKILGNKATF SPIVTLEPRRRKFHKPITMTIPVPKAS SDGIMNGYGGDTPTLRLLCSITGGTT PAQWEDITGTTPLTFVNECVSFTTNV SARFWLIDCRQTQESVTFASQVYREII CVPYMAKFVVFAKSHDPIEARLRCF CMTDDKVDKTLEQQENFAEVARSR DVEVLEGKPIYVDCFGNLVPLTKSG QHHIFSFFAFKENRLPLFVKVRDTTQ EPCGRLSFMKEPKSTRGLVHQAICNL NITLPVYTKESESDQEQEEEVDMTSE KNQQDDRERTEERLAHIADHLGFSW TELARELDFTEEQIHQIRIENPNSLQD QSHALLKYWLERDGKHATDTSLTQC LTKINRMDIVHLMETSGIDSMQVHG TRTYTEIEQTIGLDHSEGFSVLQEELY SSRHKPDERHRISKDGDPTEHPPIVSE EDVSVSYSPFQDSTPRSEAELSMAEL LRQTHKEQVEAEFSGKPQDVIETTSS QHEYFVTTPGTEQRAASDTSARFSAT KEEREKTSPQSPSSAQRGGSPIIQEPE ELHLHQDDPSPRRTSLVIVESIDEQPE KLGSGYEEESLEKELAEELGELENSS DEDEMVTTRVVRRRVIIQADSMPEM PPETVTEEQYTDEHGHTVVKKVTRK IIRRYVSPDGTEKEDIIMQGTPQKPVT VEEGDGYSKVVKRVVLKSDSEQSEV TLSEPGVLPSASNFQSEPVEGRKVSK VIKTTVVQGERMEKHLGDASLATDL PSAKEDFEEALSYTGNQIKIQLPALV EKEIMKEDGSIIKRTTLSKASTQKRT VMKDRYGKHVHIEELDDTPEALPQD DLQHDLQQLLRHFCKEDWKQEAK SEQ ID A0A287AUI5 Ankyrin 2/Sus EGQSQDKGSKSGSSIQSLFFFSQSDSN NO: 189 (UniProtKB) scrofa ASFLRAARAGNLDKVVEYLKGGIDI NTCNQNGLNALHLAAKEGHVGLVQ ELLGRGSSVDSATKKGNTALHIASLA GQAEVVKVLVKEGANINAQSQNGFT PLYMAAQENHIDVVKYLLENGANQS TATEDGFTPLAVALQQGHNQAVAIL LENDTKGKVRLPALHIAARKDDTKS AALLLQNDHNADVQSKMMVNRTTE SGFTPLHIAAHYGNVNVATLLLNRG AAVDFTARNGITPLHVASKRGNTNM VKLLLDRGGQIDAKTRDGLTPLHCA ARSGHDQVVELLLERGAPLLARTKN GLSPLHMAAQGDHVECVKHLLQHK APVDDVTLDYLTALHVAAHCGHYR VTKLLLDKRANPNARALNGFTPLHI ACKKNRIKVMELLVKYGASIQAITES GLTPIHVAAFMGHLNIVLLLLQNGAS PDVTNIRGETALHMAARAGQVEVVR CLLRNGALVDARAREEQTPLHIASRL GKTEIVQLLLQHMAHPDAATTNGYT PLHISAREGQVDVASVLLEAGAAHS LATKKGFTPLHVAAKYGSLDVAKLL LQRRAAADSAGKNGLTPLHVAAHY DNQKVALLLLEKGASPHATAKNGYT PLHIAAKKNQMQIASTLLNYGAETNI VTKQGVTPLHLASQEGHTDMVTLLL DKGANIHMSTKSGLTSLHLAAQEDK VNVADILTKHGADQDAHTKLGYTPL IVACHYGNVKMVNFLLKQGANVNA KTKNGYTPLHQAAQQGHTHIINVLL QHGAKPNATTANGNTALAIAKRLGY ISVVDTLKVVTEEVTTTTTTITEKHK LNVPETMTEVLDVSDEEGDDTMTGD GGEYLRPEDLKELGDDSLPSSQFLDG MNYLRYSLEGGRSDSLRSFSSDRSHT LSHASYLRDSAMIDDTVVIPSHQVSA LAKEAERNSYRLSWGTENLDNVALS SSPIHSGFLVSFMVDARGGAMRGCR HNGLRIIIPPRKCTAPTRVTCRLVKRH RLATMPPMVEGEGLASRLIEVGPSG AQFLGKLHLPTAPPPLNEGESLVSRIL QLGPPGTKFLGPVIVEIPHFAALRGK ERELVVLRSENGDSWKEHYCEYTED ELNEILNGMDEVLDSPEDLEKKRICR IITRDFPQYFAVVSRIKQDSNLIGPEG GVQAVFPEGALTKRIRVGLQAQPMH SELVKKILGNKATFSPIVTLEPRRRKF HKPITMTIPVPKASSDVMLNGFGGD APTLRLLCSITGGTTPAQWEDITGTTP LTFVNECVSFTTNVSARFWLIDCRQI QESVTFASQVYREIICVPYMAKFVVF AKSHDPIEARLRCFCMTDDKVDKTL EQQENFAEVARSRDVEVLEGKPIYV DCFGNLVPLTKSGQHHIFSFFAFKEN RLPLFVKVRDSTQEPCGRLSFMKEPK STRGLVHQAICNLNITLPIYTKESESD QEQEEEVIVRHYDETESTETSVLKSH LVNEVPVLASPDLLSEVSEMKQDLIK MTAILTTDVSDRAGSLKVKELVKAA EEEPGEPFEIVERVKEDLEKVNEILRS GTCAGDEGSEPRSQPEREVVEEEWVI VSDEEIEEAKRKAPLEITEYPCVEVRL DKDTKVKVEKDSLGLVNYLTEDLNS YVPPHGEPLQMEREKQEALGPGRSS ESEGKDAPSEETQSTQKQPKPSLGIK KPVRRKLKDKQKQKEDSSQASADKS ELKKGSSEESLDEDTGLAPEPLPAVK ATSPLIEETPIGSIKDKVKALQKRVED EQKGRSKLPVRVKGKEDVPKKITHR TQLAASPSLKSERHALASKPERHSSL SSPAKTERHPPVSPSSKTEKHSPVSPS AKTERHSPVSSSSKTEKHSPVSPSTKP DRHSPVSSATKTERHPPVSPSGKTDK RPPVSPSGRTEKHPPVSPGRTEKRLP VSPAGRMERHSPMSTSGKTEKHLPV SPSGKTDKQPPVSPTSKTERIEETMSV RELMKAFQSGQDPSKHKTGLFEHKS AKQKQPQEKGKVRGEKEKGLTVTQ KETQKTETQTIKRSQRFLVTGPQNPE EQPVVKREEGAGERGKALNHKTPEP VQSAPEEESHKVESPKEKLADEQGD MDLQISPDRKTSTDFSDVIKQELEDN DKYQEFCHNQVLTSPFNTTFPLDYM KEEFLPALSLQSGALDGSSESLKHEG AAGSPCGSLMEGTPQISSEESYKHEG LAETPETSPESLSFSPKKTEEQTEETK ETTKVESPPEIHSEKEDPSTKDVTDSS AGQGAVVTGGTEPSAECLLKEATLE PSKDTCPQPEDEGLDSQGESLAKETP KGLTEGVPHGEDPLTHVSSAHEKQT DTEAQKSTASKPSDETAASPLPDAVV KTSPGTESKPQGVIRSPQGLELALPSR DSEVFSPGADESFAVSHKDSLEASPV LEDNSSHKTPDSLEPSPLKESPCRDSL ESSPVEPKMKAGILPSQFPLPSAVAK TELITEVASMRSRLLRDPDGSAEDDS LEQTSLMESSGKSPLSPDTPSSEEISY EVTPKTTDAGTPKPAVIPECAEEDDL ENGEKKRFTPEEEMFKMVTKIKISKQ KRDYKKEPKQEDSSSSSDADVECSA DLDEPKCMESVEDKSNAPVVVTAES RKASSSSESEPELTQLKKGADSGLLP EPVIRVQPPSPLPSSIDSNSSPEEVQFQ PIVSKQYTFKMNEDTQEDSGKSEEEE GCESHLPEDSHTVSTSGPEMSYDDV NRDADQPKICGSYGCEAESPSSSAIP VTLGLHSSTGEDVHEQLVIHKEPLAL QDTGEKDTEGEELDVSRVEAPQVGY PTESSSSSSSLPHCPASEGKELDEDVV STSSATKMEVTKADQAFESLPDDYS TQDLPNTTQTDSSSLDVPVSDPTETD DISDPQVTSPYENVPSQSFFSSEESKT QTDTRHTSFHSSEVCSVTTTSSVEEV VVTSSSGRTVSSQESNLEDQNLECKQ ESPLCEVQPDGAPSSLEPAAPTTSTV VGEQISKVIITKTDVDSDSWSEIREDD EAFEARVKEEEQKIFGLMVDRQSQG TTPDTTPARTPTEEGTPTSEQNPFLFQ EGKLFEMTRSGAIDMTKRSYGDESF HFFQIGQESREETLTEDMKEGGTGTE LPQLETSAESLAPLESKETVNDEADL VPDDLSEEVEEVPTSDGQLNSQMGIS ASTETSMKEAASAGAEDLPCIQMSD TPSLSLVKQAALQLDFSTVTRSVYSD RDDESPDSSPEEQKSVIEIPTAPMENV PSTESRSKIPVRTMPTSTPAFPSMECE SAPSEGFLPSLDEESQEDETKPKSKIP VKAPVPRVEQQLLHLDSSLQETVAP QGQDVTSRAPDSRSKSESDASPLDA KITCPEKTGSYTETDLESSEGTEELEL ESEDETTRPKIFASRLPVKSKSTTSSC RGATSPTKESKEHFFDLYRNSIEFFEE ISDEASKLVDRLTQSEREQELVSDDE SSSALEVSVIENLPPVETEQSIPEDIFD TRPIWDESIETLIERIPDENGHDHAED QQDEQERIEERLAYIADHLGFSWTEL ARELDFTEEQIHQIRIENPNSLQDQSH ALLKYWLERDGKHATDTNLIECLTK INRMDIVHLMETITEPLQERISHSYAE IEQTITLDHSEGFSVLQEELCTAQNK QKEEQAASKEGESYDHPPIVSEEDIS VGYSTFQDSIPKTEGDGSVTELLPKT HEEQVQQDFSGKMQDLPEESSLEQQ eyfvttpgteasetpkataapgspsk TPEEIITPPEEERPYLQTPTASEQGDSP IVQEPEEPPEHREASLPQKTSLVIVES ADDQPQTFERLDEDAAFQKGDDMP DIPPETVTEEEYVDEHGHTVVKKVT RKIIRRYVSSDGTEKEEMTMQGAPQ DPISIEEGDGYSKVIKRVVLKSDTERS EVRADFVERCK SEQ ID P49024 Paxillin/Gallus MDDLDALLADLESTTSHISKRPVFLT NO: 190 (UniProtKB) gallus EETPYSYPTGNHTYQEIAVPPPVPPPP SSEALNGTVIDPLDQWQPSVSRYGH QQPQSQSPIYSSSAKSSSASVPRDGLS SPSPRASEEEHVYSFPNKQKSAEPSPT MTSTSLGSNLSELDRLLLELNAVQH NPPSGFSADEVSRSPSLPNVTGPHYVI PESSSSAGGKAAPPTKEKPKRNGGR GIEDVRPSVESLLDELESSVPSPVPAI TVSQGEVSSPQRVNASQQQTRISASS ATRELDELMASLSDFKFMAQGKAG GSSSPPSTTPKPGSQLDTMLGSLQSD LNKLGVATVAKGVCGACKKPIAGQ VVTAMGKTWHPEHFVCTHCQEEIGS RNFFERDGQPYCEKDYHNLFSPRCY YCNGPILDKVVTALDRTWHPEHFFC AQCGVFFGPEGFHEKDGKAYCRKD YFDMFAPKCGGCARAILENYISALNT LWHPECFVCRECFTPFINGSFFEHDG QPYCEVHYHERRGSLCSGCQKPITGR CITAMGKKFHPEHFVCAFCLKQLNK GTFKEQNDKPYCQNCFLKLFC SEQ ID F1MFD1 Paxillin OS = Bos ALLADLESTTSHISKRPVFLSEETPYS NO: 191 (UniProtKB) taurus YPTGNHTYQEIAVPPVPPPPSSEALN GSVLDPLDPWPPSTSRFTHQQPQSSS PVYGSSAKTSSASNPQDGGGPPCPRA GEEDHVYSFPNKQKSAEPSPTVMSSS LGSNLSELDRLLLELNAVQHNPPGFP ADEANSSPPLPGPLSTHYGVPENNSL LGGKAGALTKEKPKRNGGRGLEDLR PSVENLLDELESSVPSPVPTITVNQGE MSSPQRVTSSQQQTRISASSATRELD ELMASLSDLSKIQDLEQRADGELCW AAGWPLNGRQSGPEGQDMGGFMAQ GKTGSSSPPGGPPKPGSQLDSMLGSL QSDLNKLGVATVAKGVCGACKKPIA GQVVTAMGKTWHPEHFVCTHCQEEI GSRNFFERDGQPYCEKDYHNLFSPR CYYCNGPILDKVVTALDRTWHPEHF FCAQCGAFFGPEGFHEKDGKAYCRK DYFDMFAPKCGGCARAILENYISAL NTLWHPECFVCRECFTPFVHGSFFEH EGQPYCEAHYHERRGSLCSGCQKPIT GRCITAMAKKFHPEHFVCAFCLKQL NKGTFKEQNDKPYCQNCFLKLFC SEQ ID A0A287BTJ4 Paxillin/Sus DALLADLESTTSHISKRPVFLSEETPY NO: 192 (UniProtKB) scrofa SYPTGNHTYQEIAVPPPVPPPPSSEAL NGSVLDPIDQWQPSTSRFIHQQPQAP SPVYGSSAKTSSSSNPQDGIGLPCPRA SEEEHVYSFPNKQKSAEPSPTVMSSS LGSNLSELDRLLLELNAVQHNAPGFP TDEANSSPPLPGALSPHYGVLEHNSS LGGKAGPVTKEKPKRNGGRGLEDV RPSVESLLDELESSVPSPVPAITLNQG EMNSPQRVTSSQQQTRISASSATREL DELMASLSDFKFMAQGKTGSSSPPG GPPKPGSQLDSMLGSLQSDLNKLGV ATVAKGVCGACKKPIAGQVVTAMG KTWHPEHFVCTHCQEEIGSRNFFERD GQPYCEKDYHNLFSPRCYYCNGPIL DKVVTALDRTWHPEHFFCAQCGAFF GPEGFHEKDGKAYCRKDYFDMFAP KCGGCARAILENYISALNTLWHPECF VCRECFTPFVNGSFFEHDGQPYCEVH YHERRGSLCSGCQKPITGRCITAMAK KFHPEHFVCAFCLKQLNKGTFKEQN DKPYCQNCFLKLFC SEQ ID L8IF19 Telethonin/Bos MATSELSCLVSEENCERREAFWAEW NO: 193 (UniProtKB) mutus KDLTLSTRPEEGCSLHEEDTQRHETY HRQGQCQAL VQRSPWLVMRMGILGRGLQEYQLP YQRVLPLPIFTPAKVGTKEEREETPIQ LQELLALET ALGGQCVDRQDVAEITKQLPPVVPV SKPGTLRRSLSRSMSQEAQRG SEQ ID P81947 Tubulin alpha- MRECISIHVGQAGVQIGNACWELYC NO: 194 (UniProtKB) 1B chain/Bos LEHGIQPDGQMPSDKTIGGGDDSFNT taurus FFSETGAGKHVPRAVFVDLEPTVIDE VRTGTYRQLFHPEQLITGKEDAANN YARGHYTIGKEIIDLVLDRIRKLADQ CTGLQGFLVFHSFGGGTGSGFTSLLM ERLSVDYGKKSKLEFSIYPAPQVSTA VVEPYNSILTTHTTLEHSDCAFMVDN EAIYDICRRNLDIERPTYTNLNRLISQI VSSITASLRFDGALNVDLTEFQTNLV PYPRIHFPLATYAPVISAEKAYHEQLS VAEITNACFEPANQMVKCDPRHGKY MACCLLYRGDVVPKDVNAAIATIKT KRSIQFVDWCPTGFKVGINYQPPTVV PGGDLAKVQRAVCMLSNTTAIAEA WARLDHKFDLMYAKRAFVHWYVG EGMEEGEFSE AREDMAALEKDYEEVGVDSVEGEG EEEGEEY SEQ ID O57613 Paranemin/ MLSMEGFVGARALGEESLQMWDLN NO: 195 (UniProtKB) Gallus gallus KRLEAYLARVKFLEEENEGLRAEIQS TKENPAGDPRRARYEEELRSLRDAL HRAFTEKCAAELARDNLYEEVQHVR SRCQKEQAAREEAKRQLSSSKKELE EERRAQIWLKERAVQLEKEVEALLE VHEEEKAGLDQELASFSQSLEGFRCA PVAFQPVEVEDYSKRLSEIWRGAVE TYKAEVSQLERALGQAKENLWQVA EDNQQSQLQLRHLEKELVGLKVRKE MLEESLGQQWQEQHGEAEKFQLAIE ALEQEKQSLQVQIAQVLEDRQQLMH LKMSLSLEVATYRTLLEAESTRLQM PPGEFKLANSLRDVKLEASSSKHRAS LAAFPRPEGVAQLCRTPGDALKVLT PKSKSSSALEFQKISSVLQAPRSWEP AAPSPTVPVVSPEPGSGGAESPVHEC GAGKESPMLSPLSPEQLVNHALQDA LKEMQDDAEAKEVPTLSATQSTRDG DLEATMEEEEAAGTQGVGAEGETVS PPGLCFCSNEPTLLSATQSDVESQEE MWEEERSKEEMLNPLSSMESQEPGG EPWGGVTRRSRLQVGKEDMEATSTE ALHISEKKEQREIWSPSREDEECEFPD EEREMQEEGSLQMEIEAACAVPVGS HPVLPTGIHLQEDFLEREQESEHQET SLGELGAAAGEEREQEVCQELKASSI EEAMPAAEGSSGSGEGTTGRESTGR ARDDEGEEEDKGREALGEDDLQAGE ALGAKELGKESMGLEEAEGMWEES VDLQEEHRDLQEGHGDLQVEHEDL WEEHGHIQEEHGDTQEEYGDTQEEH GDLQVEGGDLQEEHGDTQEEHGDL QEEHGDTQEEHGDLQVEHEDLQVEH GDLWEEHRDVQEEHGDTQEEYGDT QEEHGDLQVEGGDLQEEHGDTQEEH EDLQEEHGDTQEEHGDLQVEHGDLQ EEHRDLQEGHGDLKEEHGDLQVEHE DLQVEHGDLQEEHGDTQEEHGDLQE EHGDLQVEHEDLQVEHGDLQVEHG DLQEEHGDTQEEHRDLQEVHGDQQ EEHRDLQEGHGDLQEEHGDLQVEH GDLQEEYGDTQEEHRDLQEVHGDQ QEEHRNLQEGHGDLQEGHGDLQEE HGDLWKEHGVLKEEHGDLQEGHGD LQEESGDPQEEPGEPWVQHGEQGSA GDGLEQDMVLQPGEGAWGREDNDI SQKQQAQDWEGTAEDEEETGVNTIT SQEPTQVDDNPHAEAAENEERDVTS PTAMEETQEGEDEGDAGSEVQSQQQ PQDTPGQEAEPAPGQKEVRYGDTGE APGDPQEPAEALEVEDEELSSEPIELE RGSPDTAVMQQDLGNGAESDEPME EDFQSEDAQLEEPKACRMELEDTLL NSTPLCAYSGEMLESDPNPPSSGGDG EAAPEMAQEEEGDLRGSDEAAVHAE PESCEELSPAPECTEEEEGYFIVSAPS QEGSSMEEAENSEEFEEIKVEAAEDR KDELTAPGVASLVPEDEGHSEPFVGE AEDVKMPLGEFEMPKEEDEEDAGGF AAELEEGLTVPVAEGLSEGHTDKTT LGDEGLGEEDVQDGDNPPATETSDT DPSNTDPPPGTMLEHGAGMEAAEYL PDVPTQLPVDIMKDSDILEIVEQALEF NQELVLGAKLAKDGQEEDGDAQPP QEEEGGSSPTSSCDEQPTVQEAVAEP ERTKNGEQNGLHRQASLEDLAEFTE EGLNGITHPGEAPAAHTLPLPSKHSG AEPVPELSPLQTTSCARSRSPGPLGRS DAVGPHSPATIGRLP SEQ ID E7EYD0 Myomesin 1a MSESLKLQKEQEERDLETRYESSYH NO: 196 (UniProtKB) (skelemin)/ QSSLSSVSRQSYTAYVSSHGHKISGK Danio rerio KKEKKLTPLSKKEKRSYLAVDKEDE VIGYVVPVFRSSHLATQDLMETQEE VKEEGVGYVVMRNLFARESGFKMR TVKKTRETSMRESAERMALSQKLHE KEQFQKKMNPDSLTHPPEFIVKPRGQ TVWEGKSVTLHCTVAGWPKPRVAW YKNNVLIDAKARPEKYFTESQYNMH SLEIKNCTFSDTAEYRISALNVKGESS AFAPVIIKRFKEEEELVERPHGYSPEY GVTFNTTIIDKFDVSFGREGETMSLG CTVIVYPTVKRYQPEIVWYRNGVAL SSSKWVHMHWSGEKATLTLVHLNK EDEGLYTLRVNTKSGFDTHSAYVFV RDADVEEEGVPVAPLDVRCHDANK DYVVVTWKQPAVEGSSAITGYFIDR LEVGNHHWTQCNDTPVKYARFPVT GLIEGRSYMFRVRALNNAGVSRPSR VSDPVVAMDPSDRARLRAGPSAPWT GVIKFTEEDPTVGVIPGPPTDLAVTE ATKSYVVLSWKPPVQRGHEGVMYY VEKCLVGTDAWQRVNTGIPVKSPRF ALFDLAEDKSYTFRVRSCNSAGVGE PSEETGATTVGDRLDLPSAPGPVIPIR NTDSSVVVCWGASKEVKDLVGYYIE VTVDGSGVWAPCNNKPVKGTRFVC HGLNATDKCTFRVKAVNAAGYSGSS AESEACLVKASIAVPSPPTGVTTLER LRDYMVIGWQAPAKTGGADIRGYY LDYRTVKDGVTSKWHELNLKAVTSs PYKVTDLKENEFYQFQVRAFNQAGI SEASIPTAPLECKEWTVAVPGPPHGL RVQEVRKDSMVVLWEPPTFNGRSPV TGYYVDFKEENGRWRCVQERSTKH TYMKVSGLQEGISYRLRIHAKNLAG VGVPSKATDAILAETRPGTNEIVVDV DDNGVISLIFECSDLKEDSQFVWSKN YEAFTDSSRLTIQTTGGKSRAIFNDPS LDDLGIYSCVVTNTDGVSASYTLTEE GLKRLLDISHDHQFPIIPFKSEMAIEL QEKGRVRFWAEVGKFTSNLQVEYVF NDNVIHEGKKYTMNFNKSTGIIEMF MDLLEVTDEGTFTFNLVDGKATGRT SLVLIGEEFAELQKKSEFERAEWVRR QGPHFVEYLSFEVTPECDVHLKCKV GNIKPATEIAWFKDGIEIEEDDEDAK KIGKSDEVLTFDIGKLVIKSEKAERK KKPATEESPSKPKISKKDAGVYEVKL KDERGKDKTLLNLTDAGYQAVLNE VFRVIANSSTELKVMSTEHGIILYSFV VHYLEDLRVGWLHKESKISHSDRVQ CGVTGEQLWLKINEPTEKDKGKYAI DIFDGKGSVKRVLDLSGQVWEEAFE EFKRLKAAAIAERNRARVVGGLPDV VTIQEGKSLNLTGNVWGDPAPEVSW IKNEKPLVCDEHHTLKYEHSKFASITI AAVTTTDSGKYALLVKNKYGTEAA EFTVSVYIPEDEAEKKE

7.1. Table 8

TABLE 8 Sequences of codon-optimized genes SEQ ID Protein Donor NO name animal Host DNA sequence SEQ ID Cofilin 2 Chicken S. cerevisiae ATGGCATCAGGCGTCACAGTGAACGATG NO: 197 AAGTTATCAAGGTTTTCAACGATATGAA AGTTCGTAAGTCTAGCACCCCAGAGGAA ATCAAAAAGAGAAAAAAAGCTGTCTTGT TTTGTTTATCCGATGACAAAAAGCAGAT TATTGTAGAGGAAGCTACTAGAATCCTT GTGGGTGATATAGGTGACACTGTAGAGG ATCCTTACACTGCCTTCGTCAAGTTGTTA CCATTAAATGATTGCAGATATGCTCTCTA CGACGCTACATACGAAACCAAGGAATCT AAAAAAGAGGACTTGGTTTTCATCTTTT GGGCCCCTGAATCCGCGCCACTGAAGAG TAAGATGATATACGCATCTTCAAAGGAT GCAATTAAAAAAAAGTTCACAGGTATTA AGCATGAATGGCAAGTTAACGGGCTTGA TGATATTAAAGATAGATCTACATTGGGT GAAAAGCTAGGCGGAAATGTTGTGGTTT CATTGGAAGGAAAGCCACTATAA SEQ ID Profilin Chicken S. cerevisiae ATGGCTGGCTGGCAATCTTATGTGGATA NO: 198 ACTTAATGTGTGATGGATGTTGTCAAGA GGCTGCAATCGTGGGTTACTGCGACGCA AAATACGTTTGGGCAGCAACAGCTGGGG GCATATTCCAATCAATTACACCAGTTGA AATTGATATGATCGTTGGTAAAGATAGA GAGGGATTTTTCACTAATGGTCTAACTTT AGGTGCCAAAAAGTGCAGTGTTATCAGA GACTCACTGTACGTAGACGGGGATTGCA CCATGGATATTCGTACAAAGTCTCAGGG TGGAGAACCTACATACAACGTCGCGGTC GGCAGAGCCGGGAGAGTTTTGGTTTTCG TAATGGGCAAGGAAGGTGTCCATGGTGG TGGACTTAACAAAAAGGCCTACTCTATG GCTAAGTACTTGAGAGATTCCGGTTTTTA A SEQ ID Coronin Chicken S. cerevisiae ATGTCTCGTAGAGTTGTTAGACAATCCA NO: 199 AGTTCCGTCACGTGTTCGGCCAACCAGT TAAGGCAGATCAGATGTACGAAGATATC AGAGTTTCAAAGGTTACCTGGGACTCAT CTTTTTGCGCTGTTAACCCAAAGTTCGTA GCAATAATTGTGGAAGCTGGCGGTGGGG GAGCATTTATGGTTTTACCACTAGCCAA GACTGGTAGAGTCGACAAAAATCACCCT TTAGTCACTGGACATACAGCACCTGTAT TAGATATTGACTGGTGTCCACATAACGA CAATGTTATTGCAAGTGCATCTGAGGAT ACAACTGTCATGGTATGGCAAATCCCAG ACTACGTTCCAGTAAGATCAATCACAGA ACCAGTTGTCACGCTCGAGGGTCACTCT AAGAGAGTTGGCATTATCTGTTGGCATC CTACAGCCAGAAATGTGTTGTTGTCTGC CGGTTGCGATAACTTGGTAATTCTTTGGA ACGTCGGTACAGGCGAAATGTTGCTGGC GCTTGAAGATATGCACACTGACCTCATT TACAACGTCGGATGGAACAGAAACGGGT CGTTATTAGTCACCACATGTAAAGATAA AAAGGTAAGGGTTATCGACCCTAGAAAG CAAACAGTTGTTGCGGAAATCACAAAGC CACATGATGGTGCTAGACCAATTAGAGC TATATTCATGGCCGATGGTAAGATTTTCA CAACCGGATTCTCAAAAATGTCCGAGAG ACAACTTGGGTTGTGGGATCTTAAAAAC TTCGAGGAACCAATTGCTCTGCAGGAAA TGGATACTAGTAATGGTGTTTTGTTACCA TTTTACGACCCAGACACAAACATCGTTT ACCTCTGCGGCAAGGGTGATAGTAGCAT CAGATATTTTGAGATAACAGATGAAGCT CCTTACGTCCATTACTTGAATACTTACTC CTCAAAGGAACCACAGAGAGGTATGGG ATTCATGCCAAAGCGAGGACTAGATGTT TCTAAGTGTGAAATCGCTAGATTTTTCAA GTTACATGAGAGAAAATGCGAACCTATT GTGATGACAGTGCCTAGAAAATCTGATT TGTTCCAAGATGATCTATATCCAGATACT CCTGGCCCAGAACCAGCCCTTGAAGCTG ATGAATGGTTATCTGGTAAAGATGCAGA GCCAATACTAATTTCTCTTAGAGATGGG TACGTCCCAGTGAAAAACAGAGAGTTGA AAGTTGTTAAAAAAAATATTTTGGATAG CAAGCCTCCTCCAGGTCCTCGTAGATCTC ACTCCACATCAAACACCGATATATCAAC ACCAGCTTTGGATGAAGTTTTAGAGGAA ATCCGGGTGTTGAAGGAAACTGTACAAG CACAAGAGAAGAGAATCTCAGCACTGG AACATAAGCTATGTCAATTTACTAATGG TACCGACTAA SEQ SEQ Myozenin Turkey S. cerevisiae ATGCCTTTAGCCGGAACCCCAGCACCAT ID NO: 1 TGAAGAGAAAAAAGCCAACAAAACTTA 200 TTGGTAAGCTGACACACGAAGTTAT GCCACAGGAAGTTACCAAGTTGAATCTA GGTAAAAAGATTTCTATCCCTAGAGATG TCATGTTGGAAGAGTTATCGTTAT TGACGAACAAAGGTTCCAAAATGTTCAA GTTGAGACAATTAAGAGTCGAGAAATTC ATTTACGAAAACAATCCAGACGCA TTCTCCGATAACAGTGTTGATCATTTTCA ACGTTTTATCCCATCTGGTGGACATTATG GTGAAGATGCCCATGGGTACGG TCATGGTCGTATGGTTGGGGGCGTTACA GCCGGGCAACATGGTTCATCAAAGCAAC ATTACAGTACCGTGCCTCCTCGAC CTGGTTCTAAGGGTGGTCCAGGTAACTC TGAGGGTGAACATGCTGAAAAGTCAGCT GGGTCTGCTGGAGAGGGCGGCCAC GGTACAGAAAAGGATGGTAAGAGTGGT GGCAAAAAGCCTCTACTTAAGACTTACA TCAGCCCATGGGAGAGAGCGATGGG AATCTCACCAGAGGATAAGAGCCAGTTA ACTATTGATCTTCTATCATATTCACCAAA GGCAGACTTCCCACACTACAAAT CTTTTAACAGAACAGCAATGCCATACGG CGGATACGAAAAAGCTGCTAAGAGAAT GACATTTAAGGTACCTCAATTCGAT ATCTGTCCACTGTTGCCAGAATCCATAGT ACTCTACAACCAAAATTTCAGAAACAGA CCATCATTCAATAGAACTCCTAT ACCTTGGATGCCATCTGGCGAATCTTCC GAATACCACACTGACATTAACGTGCCAA GATCTGGAGAAACAGAGGAATTGT AA SEQ ID Troponin Pig S. cerevisiae ATGACTGATCAACAAGCTGAAGCAAGAT NO: 201 C, CTTACCTTAGTGAAGAGATGATAGCAGA skeletal GTTTAAGGCAGCGTTCGATATGTTCGAT muscle GCCGACGGTGGTGGCGATATCTCTGTGA AGGAACTCGGTACAGTTATGAGAATGCT GGGGCAAACACCAACCAAGGAAGAGTT GGATGCAATCATCGAAGAGGTCGACGAA GATGGGTCAGGTACAATTGATTTTGAAG AGTTTTTGGTTATGATGGTAAGACAGAT GAAAGAGGATGCTAAGGGTAAGTCAGA AGAGGAATTAGCTGAATGTTTTAGAATT TTCGATAGAAATGCTGATGGATACATTG ACGCTGAGGAACTAGCCGAAATTTTCCG TGCCTCTGGAGAACATGTCACTGATGAG GAATTGGAATCCTTAATGAAAGATGGCG ACAAAAACAACGAGGGTAGAATCGACTT CGACGAATTCCTTAAGATGATGGAAGGC GTTCAATAA SEQ ID Cofilin 2 Chicken K. phaffii ATGGCTTCTGGTGTGACTGTTAACGACG NO: 202 AAGTCATCAAGGTATTCAATGATATGAA AGTTAGAAAATCATCCACTCCAGAGGAA ATCAAAAAGAGAAAAAAAGCCGTTCTAT TTTGCCTGTCGGACGACAAAAAGCAGAT CATCGTTGAGGAAGCCACACGTATTTTG GTCGGTGACATTGGTGACACAGTCGAAG ATCCTTATACTGCTTTTGTTAAGCTGTTG CCCTTAAATGATTGTAGGTACGCTCTGTA CGACGCAACTTACGAAACCAAAGAGTCC AAAAAAGAGGATTTGGTGTTCATCTTCT GGGCACCTGAAAGTGCTCCACTTAAGAG CAAGATGATTTATGCATCCTCTAAAGAT GCTATTAAAAAAAAGTTTACAGGTATAA AGCATGAGTGGCAAGTGAACGGATTGGA TGACATTAAAGATAGATCTACGTTGGGC GAAAAGCTTGGTGGAAATGTTGTAGTGT CATTAGAGGGAAAGCCACTCTAA

8. EQUIVALENTS

While various specific embodiments have been illustrated and described, the above specification is not restrictive. It will be appreciated that various changes can be made without departing from the spirit and scope of the invention(s). Many variations will become apparent to those skilled in the art upon review of this specification.

9. REFERENCES CITED

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10. INCORPORATION BY REFERENCE

All publications, patents, patent applications and other documents cited in this application are hereby incorporated by reference in their entireties for all purposes to the same extent as if each individual publication, patent, patent application or other document were individually indicated to be incorporated by reference for all purposes. 

1.-176. (canceled)
 177. A pet food or animal feed composition comprising a yeast cell or filamentous fungal cell that expresses at least one recombinant animal protein.
 178. The composition of claim 177, wherein the composition is substantially free of antibiotics, animal growth hormones, and animal meat.
 179. The composition of claim 177, wherein the recombinant animal protein has a maximum amino acid sequence identity of 70% to any protein naturally encoded in the genome of the yeast cell or filamentous fungal cell that expresses the recombinant animal protein.
 180. The composition of claim 177, wherein the recombinant protein is profilin, cofilin, coronin, myozenin, troponin, radixin, myotubularin, gamma-sarcoglycan, alpha-actinin, caveolin, desmin, tropomyosin, titin, connectin, transgelin, smoothelin, actin or myosin.
 181. The composition of claim 177, wherein the recombinant animal protein is a protein of a chicken, a pig, a turkey, a pheasant, a quail, a horse, cattle, a fish, a shrimp, a prawn, a crab, a sheep, a deer, a duck, a rabbit, an elk, a moose, a kangaroo, an alligator, a wild boar, a goat, a bison, a buffalo, a bear, or an elephant.
 182. The composition of claim 177, wherein the recombinant animal protein is a poultry protein, even-toed ungulate protein, or fish protein.
 183. The composition of claim 177, wherein the recombinant animal protein is a cytoskeletal protein.
 184. The composition of claim 177, wherein the recombinant animal protein is a muscle protein.
 185. The composition of claim 177, wherein the recombinant animal protein is a skeletal muscle protein.
 186. The composition of claim 177, wherein the composition comprises a yeast cell or a filamentous fungal cell that expresses at least two different recombinant animal proteins.
 187. The composition of claim 177, wherein the composition comprises at least two different yeast or filamentous fungal cells that each express at least one different recombinant animal protein.
 188. The composition of claim 177, wherein the composition comprises about 1% to 30% of the recombinant protein based on the dry weight of the composition.
 189. The composition of claim 177, wherein the composition comprises about 5% to 40% of the recombinant protein based on the dry weight of the composition.
 190. The composition of claim 177, wherein the composition further comprises a fat, a carbohydrate, a non-recombinant protein, a fiber, a nutritional supplement, a palatability agent, or any combination thereof.
 191. The composition of claim 177, wherein the composition comprises 5-50% protein, 0-50% fat, 0-75% carbohydrate, 0-40% dietary fiber, and 0-15% of other nutrients.
 192. The composition of claim 177, wherein the composition is nutritionally balanced for a companion animal, optionally wherein the composition is a kibble.
 193. A method for producing a pet food or animal feed composition according to claim 1, the method comprising culturing yeast or filamentous fungal cells that express an animal protein and processing the cells to provide the pet food or animal feed composition.
 194. The method of claim 193, wherein processing the cells comprises combining the cells with a fat, a carbohydrate, a non recombinant protein, a fiber, a nutritional supplement, a palatability agent, or any combination thereof; or dehydrating, baking, and/or extruding the yeast or filamentous fungal cells, optionally combined with a fat, a carbohydrate, a non recombinant protein, a fiber, a nutritional supplement, a palatability agent, or any combination thereof.
 195. The method of claim 194, wherein the method comprises lysing the yeast or filamentous fungal cells.
 196. The method of claim 193, wherein the method comprises isolating, purifying, and/or concentrating, the animal protein. 